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The map label for this gene is pyrG

Identifier: 39996993

GI number: 39996993

Start: 2072273

End: 2073883

Strand: Reverse

Name: pyrG

Synonym: GSU1895

Alternate gene names: 39996993

Gene position: 2073883-2072273 (Counterclockwise)

Preceding gene: 39996994

Following gene: 39996992

Centisome position: 54.37

GC content: 59.59

Gene sequence:

>1611_bases
ATGAAAACCAAGTTCATCTTCGTAACCGGTGGCGTTGTGTCCTCCATCGGCAAAGGGCTTGCCTCGGCGTCGCTCGGGGC
GCTGCTGGAGTCCCGCGGCCTGCGGGTAACCATGCAGAAGCTGGACCCTTACATCAACGTTGACCCCGGCACCATGTCGC
CGTTCCAGCACGGCGAGGTTTTTGTCACCGACGATGGCGCCGAGACCGACCTGGACCTGGGGCACTACGAACGGTACACC
TCTGCCCGGCTCTCCAAACGGAGCAATTTCACCACGGGCCAAGTCTATTTTTCGGTCATCGAGAAGGAACGGCGCGGCGA
TTATCTGGGGGGGACCGTGCAGGTTATTCCCCATATCACCGACGAGATCAAGCACAAGATCCTGGAGAACGCCAAAGGGG
CCGATGTGGCCATCGTGGAGGTCGGGGGAACGGTCGGCGACATCGAATCGCTCCCGTTCCTGGAGGCGATCCGGCAGTTC
AAGGCGGACCGGGGGGCGGGGAACGTTCTTTACATTCACGTGACGCTCGTGCCCCACATCAAGACGGCCGGTGAGTTGAA
GACCAAGCCGACGCAGCACTCCGTCAAGGAGTTGCGCGAGATCGGTATCCAGCCCGACATTCTCATCTGCCGTTGCGAGA
TGGAGCTGCCCCGGGACATGAAGGCCAAGATCGCCCTGTTCTGCAACGTGGAAGAGAAGGCGGTCATCACCTCTACCGAT
GCGGAACATATCTATGCGGTACCCCTGGCGCTTCACAAGGAAGGTCTCGACGAGCAGGTAGTAGAAAAGCTCAACATCTG
GACCAAGGCGCCTGATCTCAGTCCCTGGCACAGTGTTGTGGAAAAACTTCGCTCGCCCCTGCGGGGAGAGGTCCGCATTG
CCATCGTCGGCAAGTACGTTAATCTCACCGAGTCATACAAGTCCCTGTCCGAAGCCCTTACCCACGGCGGGATCGCCAAC
GACTGCCGGGTGGTTCTCACCTATCTGGACTCCGAGCGGATCGAAAGCGAGGGGATCGGCAGCTCCTTCGATGACATCGA
TGCCATCCTGGTGCCGGGAGGGTTCGGCGAGCGGGGAACCGAGGGCAAGATCAAGGCCATCGAGTACGCCCGCACCCAAA
AGATTCCTTTCTTCGGCATCTGTCTCGGCATGCAGATGGCGGTGGTGGAGTATGCCCGCAACGTTTGTGGGCTCGAAGAT
GCCTGTTCCAGCGAGTTCCGTCCCGATTGCGCCAACCCGGTCATCAGTCTGATGGAGGAACAACGCGACATCGACCGGCT
GGGCGGCACCATGCGGCTCGGTGCCTATCCCTGCAGCCTTACCAAGGGAACTTTTGCCCAAAAAGCGTACGGGTCCCTGG
AAATATCCGAACGTCACCGTCACCGCTACGAGTACAATAACGCCTTCCGTGAGACCCTTGTGGCCAATGGCCTCGTCGTG
TCGGGGCTCTACAAGGAAGGGGATCTGGTGGAGATCGTGGAGGTCGCCGACCACCCTTGGTTCCTCGGCTGCCAGTTCCA
CCCGGAATTCAAGTCAAAGCCGCTTAACCCCCACCCTCTGTTCAGGGCATTTATCGCAGCCGCCCTTGATCGGAAAGACA
AAAGGCGATAG

Upstream 100 bases:

>100_bases
CAGGGTGGTTGAAACAGCGCACGAGTCGATCGGCGTCGACACCCCCAACGACCTGGAAAAGGTTCTCGAAAAGTTGAAAT
AATCTGACTCAGGAGTGTGC

Downstream 100 bases:

>100_bases
GCTGTCAGCCTATCCCCACAGATAAGAGGTTTGCATGACCAGGGAAATTACCATAGGTTCCGTAAAGATTGGTGGCGACC
GACCGCTGGTGCTCATCGCC

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MKTKFIFVTGGVVSSIGKGLASASLGALLESRGLRVTMQKLDPYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERYT
SARLSKRSNFTTGQVYFSVIEKERRGDYLGGTVQVIPHITDEIKHKILENAKGADVAIVEVGGTVGDIESLPFLEAIRQF
KADRGAGNVLYIHVTLVPHIKTAGELKTKPTQHSVKELREIGIQPDILICRCEMELPRDMKAKIALFCNVEEKAVITSTD
AEHIYAVPLALHKEGLDEQVVEKLNIWTKAPDLSPWHSVVEKLRSPLRGEVRIAIVGKYVNLTESYKSLSEALTHGGIAN
DCRVVLTYLDSERIESEGIGSSFDDIDAILVPGGFGERGTEGKIKAIEYARTQKIPFFGICLGMQMAVVEYARNVCGLED
ACSSEFRPDCANPVISLMEEQRDIDRLGGTMRLGAYPCSLTKGTFAQKAYGSLEISERHRHRYEYNNAFRETLVANGLVV
SGLYKEGDLVEIVEVADHPWFLGCQFHPEFKSKPLNPHPLFRAFIAAALDRKDKRR

Sequences:

>Translated_536_residues
MKTKFIFVTGGVVSSIGKGLASASLGALLESRGLRVTMQKLDPYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERYT
SARLSKRSNFTTGQVYFSVIEKERRGDYLGGTVQVIPHITDEIKHKILENAKGADVAIVEVGGTVGDIESLPFLEAIRQF
KADRGAGNVLYIHVTLVPHIKTAGELKTKPTQHSVKELREIGIQPDILICRCEMELPRDMKAKIALFCNVEEKAVITSTD
AEHIYAVPLALHKEGLDEQVVEKLNIWTKAPDLSPWHSVVEKLRSPLRGEVRIAIVGKYVNLTESYKSLSEALTHGGIAN
DCRVVLTYLDSERIESEGIGSSFDDIDAILVPGGFGERGTEGKIKAIEYARTQKIPFFGICLGMQMAVVEYARNVCGLED
ACSSEFRPDCANPVISLMEEQRDIDRLGGTMRLGAYPCSLTKGTFAQKAYGSLEISERHRHRYEYNNAFRETLVANGLVV
SGLYKEGDLVEIVEVADHPWFLGCQFHPEFKSKPLNPHPLFRAFIAAALDRKDKRR
>Mature_536_residues
MKTKFIFVTGGVVSSIGKGLASASLGALLESRGLRVTMQKLDPYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERYT
SARLSKRSNFTTGQVYFSVIEKERRGDYLGGTVQVIPHITDEIKHKILENAKGADVAIVEVGGTVGDIESLPFLEAIRQF
KADRGAGNVLYIHVTLVPHIKTAGELKTKPTQHSVKELREIGIQPDILICRCEMELPRDMKAKIALFCNVEEKAVITSTD
AEHIYAVPLALHKEGLDEQVVEKLNIWTKAPDLSPWHSVVEKLRSPLRGEVRIAIVGKYVNLTESYKSLSEALTHGGIAN
DCRVVLTYLDSERIESEGIGSSFDDIDAILVPGGFGERGTEGKIKAIEYARTQKIPFFGICLGMQMAVVEYARNVCGLED
ACSSEFRPDCANPVISLMEEQRDIDRLGGTMRLGAYPCSLTKGTFAQKAYGSLEISERHRHRYEYNNAFRETLVANGLVV
SGLYKEGDLVEIVEVADHPWFLGCQFHPEFKSKPLNPHPLFRAFIAAALDRKDKRR

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=553, Percent_Identity=46.2929475587703, Blast_Score=488, Evalue=1e-138,
Organism=Homo sapiens, GI28559085, Length=550, Percent_Identity=45.0909090909091, Blast_Score=475, Evalue=1e-134,
Organism=Homo sapiens, GI28559083, Length=550, Percent_Identity=45.0909090909091, Blast_Score=475, Evalue=1e-134,
Organism=Homo sapiens, GI221316689, Length=550, Percent_Identity=45.0909090909091, Blast_Score=475, Evalue=1e-134,
Organism=Escherichia coli, GI1789142, Length=538, Percent_Identity=55.5762081784387, Blast_Score=603, Evalue=1e-173,
Organism=Caenorhabditis elegans, GI25148299, Length=603, Percent_Identity=38.4742951907131, Blast_Score=415, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6319432, Length=563, Percent_Identity=42.8063943161634, Blast_Score=439, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6322563, Length=563, Percent_Identity=42.6287744227353, Blast_Score=432, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24664469, Length=554, Percent_Identity=44.2238267148014, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI21357815, Length=499, Percent_Identity=42.4849699398798, Blast_Score=389, Evalue=1e-108,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_GEOSL (Q74BY3)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_952944.1
- HSSP:   P17812
- ProteinModelPortal:   Q74BY3
- SMR:   Q74BY3
- GeneID:   2686232
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU1895
- NMPDR:   fig|243231.1.peg.1882
- TIGR:   GSU1895
- HOGENOM:   HBG597806
- OMA:   RVTMQKL
- ProtClustDB:   PRK05380
- BioCyc:   GSUL243231:GSU_1895-MONOMER
- BRENDA:   6.3.4.2
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 59430; Mature: 59430

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 381-381 ACT_SITE 507-507 ACT_SITE 509-509

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKFIFVTGGVVSSIGKGLASASLGALLESRGLRVTMQKLDPYINVDPGTMSPFQHGEV
CCEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHCCCEEECCCCCCCCCCCCEE
FVTDDGAETDLDLGHYERYTSARLSKRSNFTTGQVYFSVIEKERRGDYLGGTVQVIPHIT
EEECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECHHH
DEIKHKILENAKGADVAIVEVGGTVGDIESLPFLEAIRQFKADRGAGNVLYIHVTLVPHI
HHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEECCC
KTAGELKTKPTQHSVKELREIGIQPDILICRCEMELPRDMKAKIALFCNVEEKAVITSTD
CCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCEEEEECC
AEHIYAVPLALHKEGLDEQVVEKLNIWTKAPDLSPWHSVVEKLRSPLRGEVRIAIVGKYV
CCEEEEEEHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEEH
NLTESYKSLSEALTHGGIANDCRVVLTYLDSERIESEGIGSSFDDIDAILVPGGFGERGT
HHHHHHHHHHHHHHHCCCCCCCEEEEEHHCHHHHHHCCCCCCCCCCCEEEECCCCCCCCC
EGKIKAIEYARTQKIPFFGICLGMQMAVVEYARNVCGLEDACSSEFRPDCANPVISLMEE
CCCEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHH
QRDIDRLGGTMRLGAYPCSLTKGTFAQKAYGSLEISERHRHRYEYNNAFRETLVANGLVV
HHHHHHCCCEEEECCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEE
SGLYKEGDLVEIVEVADHPWFLGCQFHPEFKSKPLNPHPLFRAFIAAALDRKDKRR
EEECCCCCEEEEEECCCCCEEEEEEECCHHCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKTKFIFVTGGVVSSIGKGLASASLGALLESRGLRVTMQKLDPYINVDPGTMSPFQHGEV
CCEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHCCCEEECCCCCCCCCCCCEE
FVTDDGAETDLDLGHYERYTSARLSKRSNFTTGQVYFSVIEKERRGDYLGGTVQVIPHIT
EEECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECHHH
DEIKHKILENAKGADVAIVEVGGTVGDIESLPFLEAIRQFKADRGAGNVLYIHVTLVPHI
HHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEECCC
KTAGELKTKPTQHSVKELREIGIQPDILICRCEMELPRDMKAKIALFCNVEEKAVITSTD
CCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCEEEEECC
AEHIYAVPLALHKEGLDEQVVEKLNIWTKAPDLSPWHSVVEKLRSPLRGEVRIAIVGKYV
CCEEEEEEHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEEH
NLTESYKSLSEALTHGGIANDCRVVLTYLDSERIESEGIGSSFDDIDAILVPGGFGERGT
HHHHHHHHHHHHHHHCCCCCCCEEEEEHHCHHHHHHCCCCCCCCCCCEEEECCCCCCCCC
EGKIKAIEYARTQKIPFFGICLGMQMAVVEYARNVCGLEDACSSEFRPDCANPVISLMEE
CCCEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHH
QRDIDRLGGTMRLGAYPCSLTKGTFAQKAYGSLEISERHRHRYEYNNAFRETLVANGLVV
HHHHHHCCCEEEECCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEE
SGLYKEGDLVEIVEVADHPWFLGCQFHPEFKSKPLNPHPLFRAFIAAALDRKDKRR
EEECCCCCEEEEEECCCCCEEEEEEECCHHCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA