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The map label for this gene is rpoN [H]

Identifier: 39996985

GI number: 39996985

Start: 2065151

End: 2066596

Strand: Reverse

Name: rpoN [H]

Synonym: GSU1887

Alternate gene names: 39996985

Gene position: 2066596-2065151 (Counterclockwise)

Preceding gene: 39996986

Following gene: 39996984

Centisome position: 54.18

GC content: 54.98

Gene sequence:

>1446_bases
ATGGCCATTGAAATGCGCCAACAGATGAAGATGACCCAGCAGCTGGTGATGACGCCCCAGTTGCAGCAGGCCATCAAACT
TCTCCAGCTGTCACGGCTGGAGCTTCAGGATCTGGTCCGTCAGGAGATGGAAGAGAATCCGCTTCTGGAAGAGTCTCAGG
AGGCTGAAGAGGTCAAGGAACAGGATCTGGTCGAACTTTCCGAAAAGGAGGATGCTCCCGCTCCGGCTGAACAGGATTTT
CGCGAGGTCACCACGGGGGAAGAGACCCGGGAGTCGGACTGGGACAGCTACCTTGAGGGTTACAATTACAGTTCCGGCGA
ACAGTACTACGATGATGAAGATCGTCCATCCTACGAAAACATTCTGACACGCAAAGGGACCCTTGCCGATCATCTCATGT
GGCAGCTCAATATGACCAAGTTGAGCGATGAAGAGGCACGGGCCAGCGCGGAGATTATCGGCAACATAGATGAAGACGGC
TACCTGCGCGCTACGGTTGAGGAAGTCGCCCTGGCATGCTCCGTAAGCGAATCCGTGGCGGAATCGGCTTTGAAGAAGAT
CCAGGAGTTTGACCCCATGGGGGTGGGGGCTCGTAGCCTGAGGGAATGTCTCCTGATCCAGGTGGAGCAGCTCGGCATGG
CGGGGAGCGTTGTGGACGGCATCCTCCGCAATCACCTCCACGACTTGGAGACCCGCAAGTACAAACAAATTGCCAAGTCT
CTCGGGGTCGACGTGGACAGCATCCTGATGGCCGCCAAGATTATCGCCGGCCTGGACCCGAAACCGGGCCGAGTCTATGG
CTCCGAAGATGTCCACTACATTTCGGCCGATATATTTGTCTATAAAATCGCTGACGACTATGTCGTCGTGCTTAATGACG
AAGGCCTTCCCAATCTGAGAATCAGCCCCTTTTATGCCGGCGAGATCAAAAACGGTGCCGCCGTCGACGCCAAGGCCGAG
GAGTACATCAACGAAAAGTCCCGCTCCGCCATGTGGCTCATCAAGAGCATCCACCAGCGGCAGAGAACCATCTACAAGGT
TGCGAAGAGCATTGTGAAGTTCCAGCGCGAGTTTCTCGACCGCGGGATAGAACACCTTCGCCCCCTGGTGCTCAGGGACG
TAGCCGAGGATATCGGCATGCACGAGTCGACCATCAGCCGCGTCACCACCAACAAGTACATGCAAACCCCCCAGGGGCTC
TTCGAGATGAAGTACTTTTTCAATAGCGGCATTTCAACTACCGAAGGAGATTTCATCGCTTCAGAGAGCGTCAAGAACAA
GATCAAGGAAATCGTTGATTCCGAGGATCCCCGAAAGCCGTACAGCGACCAGCGTATCGCCGAATTGCTTTCGGCGCACA
CCATCAACATCGCCCGACGAACCGTAACGAAATACCGCGAGATGTTGAAAATCGGCTCGTCATCCGAGCGCAAACGTCAT
TTTTGA

Upstream 100 bases:

>100_bases
ATTCAGACTCTAGCGGCGGCCGGATCCCGGTACTCAACCCCTTGCCGACGGGTTGCCAGCCGGTTCTGCAGCCGGATCTT
CCTTCAAAGGTGTGTTATGT

Downstream 100 bases:

>100_bases
CCAGCTGACATTTTGATTTATTCTTTCGATGTTCCGGGTTGAAGGATCGTCATTTCGTGCTTTGATTACAGTGAAGACCA
CGAAAGACGGTTGGTCCCGG

Product: RNA polymerase sigma-54 factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MAIEMRQQMKMTQQLVMTPQLQQAIKLLQLSRLELQDLVRQEMEENPLLEESQEAEEVKEQDLVELSEKEDAPAPAEQDF
REVTTGEETRESDWDSYLEGYNYSSGEQYYDDEDRPSYENILTRKGTLADHLMWQLNMTKLSDEEARASAEIIGNIDEDG
YLRATVEEVALACSVSESVAESALKKIQEFDPMGVGARSLRECLLIQVEQLGMAGSVVDGILRNHLHDLETRKYKQIAKS
LGVDVDSILMAAKIIAGLDPKPGRVYGSEDVHYISADIFVYKIADDYVVVLNDEGLPNLRISPFYAGEIKNGAAVDAKAE
EYINEKSRSAMWLIKSIHQRQRTIYKVAKSIVKFQREFLDRGIEHLRPLVLRDVAEDIGMHESTISRVTTNKYMQTPQGL
FEMKYFFNSGISTTEGDFIASESVKNKIKEIVDSEDPRKPYSDQRIAELLSAHTINIARRTVTKYREMLKIGSSSERKRH
F

Sequences:

>Translated_481_residues
MAIEMRQQMKMTQQLVMTPQLQQAIKLLQLSRLELQDLVRQEMEENPLLEESQEAEEVKEQDLVELSEKEDAPAPAEQDF
REVTTGEETRESDWDSYLEGYNYSSGEQYYDDEDRPSYENILTRKGTLADHLMWQLNMTKLSDEEARASAEIIGNIDEDG
YLRATVEEVALACSVSESVAESALKKIQEFDPMGVGARSLRECLLIQVEQLGMAGSVVDGILRNHLHDLETRKYKQIAKS
LGVDVDSILMAAKIIAGLDPKPGRVYGSEDVHYISADIFVYKIADDYVVVLNDEGLPNLRISPFYAGEIKNGAAVDAKAE
EYINEKSRSAMWLIKSIHQRQRTIYKVAKSIVKFQREFLDRGIEHLRPLVLRDVAEDIGMHESTISRVTTNKYMQTPQGL
FEMKYFFNSGISTTEGDFIASESVKNKIKEIVDSEDPRKPYSDQRIAELLSAHTINIARRTVTKYREMLKIGSSSERKRH
F
>Mature_480_residues
AIEMRQQMKMTQQLVMTPQLQQAIKLLQLSRLELQDLVRQEMEENPLLEESQEAEEVKEQDLVELSEKEDAPAPAEQDFR
EVTTGEETRESDWDSYLEGYNYSSGEQYYDDEDRPSYENILTRKGTLADHLMWQLNMTKLSDEEARASAEIIGNIDEDGY
LRATVEEVALACSVSESVAESALKKIQEFDPMGVGARSLRECLLIQVEQLGMAGSVVDGILRNHLHDLETRKYKQIAKSL
GVDVDSILMAAKIIAGLDPKPGRVYGSEDVHYISADIFVYKIADDYVVVLNDEGLPNLRISPFYAGEIKNGAAVDAKAEE
YINEKSRSAMWLIKSIHQRQRTIYKVAKSIVKFQREFLDRGIEHLRPLVLRDVAEDIGMHESTISRVTTNKYMQTPQGLF
EMKYFFNSGISTTEGDFIASESVKNKIKEIVDSEDPRKPYSDQRIAELLSAHTINIARRTVTKYREMLKIGSSSERKRHF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=479, Percent_Identity=37.7870563674322, Blast_Score=322, Evalue=3e-89,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 54842; Mature: 54710

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIEMRQQMKMTQQLVMTPQLQQAIKLLQLSRLELQDLVRQEMEENPLLEESQEAEEVKE
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
QDLVELSEKEDAPAPAEQDFREVTTGEETRESDWDSYLEGYNYSSGEQYYDDEDRPSYEN
HHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
ILTRKGTLADHLMWQLNMTKLSDEEARASAEIIGNIDEDGYLRATVEEVALACSVSESVA
HHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
ESALKKIQEFDPMGVGARSLRECLLIQVEQLGMAGSVVDGILRNHLHDLETRKYKQIAKS
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGVDVDSILMAAKIIAGLDPKPGRVYGSEDVHYISADIFVYKIADDYVVVLNDEGLPNLR
HCCCHHHHHHHHHHHHCCCCCCCCEECCCCCEEEEEEEEEEEECCCEEEEECCCCCCCCE
ISPFYAGEIKNGAAVDAKAEEYINEKSRSAMWLIKSIHQRQRTIYKVAKSIVKFQREFLD
ECCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGIEHLRPLVLRDVAEDIGMHESTISRVTTNKYMQTPQGLFEMKYFFNSGISTTEGDFIA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCHHH
SESVKNKIKEIVDSEDPRKPYSDQRIAELLSAHTINIARRTVTKYREMLKIGSSSERKRH
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCC
F
C
>Mature Secondary Structure 
AIEMRQQMKMTQQLVMTPQLQQAIKLLQLSRLELQDLVRQEMEENPLLEESQEAEEVKE
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
QDLVELSEKEDAPAPAEQDFREVTTGEETRESDWDSYLEGYNYSSGEQYYDDEDRPSYEN
HHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
ILTRKGTLADHLMWQLNMTKLSDEEARASAEIIGNIDEDGYLRATVEEVALACSVSESVA
HHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
ESALKKIQEFDPMGVGARSLRECLLIQVEQLGMAGSVVDGILRNHLHDLETRKYKQIAKS
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGVDVDSILMAAKIIAGLDPKPGRVYGSEDVHYISADIFVYKIADDYVVVLNDEGLPNLR
HCCCHHHHHHHHHHHHCCCCCCCCEECCCCCEEEEEEEEEEEECCCEEEEECCCCCCCCE
ISPFYAGEIKNGAAVDAKAEEYINEKSRSAMWLIKSIHQRQRTIYKVAKSIVKFQREFLD
ECCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGIEHLRPLVLRDVAEDIGMHESTISRVTTNKYMQTPQGLFEMKYFFNSGISTTEGDFIA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCHHH
SESVKNKIKEIVDSEDPRKPYSDQRIAELLSAHTINIARRTVTKYREMLKIGSSSERKRH
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCC
F
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113171; 10984043 [H]