Click here to switch to the map view.

The map label for this gene is ptsI [H]

Identifier: 39996979

GI number: 39996979

Start: 2060053

End: 2061819

Strand: Reverse

Name: ptsI [H]

Synonym: GSU1881

Alternate gene names: 39996979

Gene position: 2061819-2060053 (Counterclockwise)

Preceding gene: 39996980

Following gene: 308513310

Centisome position: 54.06

GC content: 58.97

Gene sequence:

>1767_bases
ATGGGTTCGGCGAGGAGTGAAACCAGGGTATTCAGCGGCATAGGTGCGTCGCCGGGGATTGCCATCGGCACTGCCCGCGT
CACCGACCGCAGCCGTGTAATGGTGGTCGAAGCCTCAGTGCCACCCGAAGATATCCCGGGTGAGGTGGTTCGATTTACCG
CTGCCCTGTCACGTTCGCGGGACGACCTGCTGGCCCTCAAGGAGTCTATCGCCGCCCGCAGGGGCGCAGAGCACCTCTAT
GTGGTTGACGCCCACCTTCTGATCCTCGACGACTCCATGCTGCGCCAGGGCACGATCGATCTCATCGAGAGGGATGGGGT
CAACGCCGAGGCGGCACTCAAGAAAAATCTCGACAAGTTCAAGGAGTTCTTTGCTGGGATCGATGACGATTACCTGCGGG
AGCGTTTCGGGGATGTGGAAACCGTGGTCGAGAGGATTCTGCGTCACATGGTCGGCAAACGTCATGAGCGTATTGACGCC
ATCGACGAGGGGAAGCGGCTCATTGTCGCCCATGACCTCTCTCCCACCGACATCCTCCAGATGGACAAGGAAAAAGTCGT
CGGTTTTGTTACCGACCTGGGTGGAAAGACCTCTCACACGTCCATATTGGCCCGCGCCTTCGAGATTCCCGCGGTTGTCG
GCCTCGAAACCATCACGGTCGAGGATATCGATGGCCTGCCGATCATCATCGACGGCACCACCGGCACCATTGTCGTCAAT
CCCTCCGAAGAGACCTTCCGCGATTACCTGCGGAGAAAACAACACTACGACTACCTCGAACAGGAACTCCTGAAGCTCAA
GGACCTGCCTGCCGTGACCCTGGACGGTCACAGCATGAGACTCATGGGGAATATCGAGTTTCCCGAGGAAATTCCTTCCC
TCAAGGGCCACGGCGGCCAGGGCGTGGGACTCTACCGCACCGAGATGCTCTACATGAATCGCCCGGGGCTCCCCGACGAA
GAAGAGCAGTACCGGGCCTATGCAACCATCGTAGAGAATGTCGCGCCCGAGCCGGTCACCATAAGGACCCTCGACATCGG
CGGAGATAAGCTGGCCACCGATCTGCACCTGGAAGACGAGATGAACCCTGCCATGGGGTTGCGCGCCATCCGTTTGTCCC
TCCGCAGGCCGGAAGTTTTCAAGTCCCAGCTGAGAGCGATACTTCGGGCCGGTTGCAAGGGGAGTGTGCGACTCTTTTTC
CCCATGATTTCAGGCATCGAAGAGGTGCGGAGCGCCAAGGCAGTTCTCCGTGAATGCATGGAAGAGCTGCGTTCGGCCGG
TATTCCCTTTGACGAGAAGATCGAGACGGGAATAATGATCGAGGTGCCGTCCGCGGTTACCATCGCCGACCTGCTGGCGC
GCGAGGTGGATTTCTTCAGTGTCGGTACCAATGACCTGATCCAGTACTCGCTGGCCATCGATCGGACTAATGAGCACCTG
GCGCATCTGTATCAACCGTTGCACCCCGCCATTCTCCGTTCCCTCAAAACCGTTGCCGATGCGGCCCATGCCGCGGGAAT
CCGTGTCTGCATGTGTGGTGAAATGGCGGGCGAACCACTCTACTTGCCAATCCTGCTTGGTCTCGGGTTTGATGAGCTGT
CGATGACAGCCGTGTCCATTCCCCGGGTCAAGAAGATTCTTCGCCGCGCCAGACGTTCCGACGCCGAGCGGTTGGTTGCC
CGCGCGCTCACCTTTGCCACCGCAGCCGAGGTGGAGTCCTTTGTTAAAAGCGAGATTTCGGCGCGTTTTTCCGAGAGTTT
CGACTGA

Upstream 100 bases:

>100_bases
ATAATGATGCTTGCCGCTGCCAAGGGGACGACCATCCGTGTCCGGGTGGCGGGCGACGATGAGGATGAGGCCATGGCCTC
ACTCGACGAGATTATCACTA

Downstream 100 bases:

>100_bases
TCGCCCCGGTTTTAGCCCTTGACTTTTCTCTCATCCACTCGATACAATCCATAGTCTTTTCCAAGCGCTACCCTTATACA
TCACACGAGGAGGAATGAAC

Product: phosphocarrier protein HPr

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 588; Mature: 587

Protein sequence:

>588_residues
MGSARSETRVFSGIGASPGIAIGTARVTDRSRVMVVEASVPPEDIPGEVVRFTAALSRSRDDLLALKESIAARRGAEHLY
VVDAHLLILDDSMLRQGTIDLIERDGVNAEAALKKNLDKFKEFFAGIDDDYLRERFGDVETVVERILRHMVGKRHERIDA
IDEGKRLIVAHDLSPTDILQMDKEKVVGFVTDLGGKTSHTSILARAFEIPAVVGLETITVEDIDGLPIIIDGTTGTIVVN
PSEETFRDYLRRKQHYDYLEQELLKLKDLPAVTLDGHSMRLMGNIEFPEEIPSLKGHGGQGVGLYRTEMLYMNRPGLPDE
EEQYRAYATIVENVAPEPVTIRTLDIGGDKLATDLHLEDEMNPAMGLRAIRLSLRRPEVFKSQLRAILRAGCKGSVRLFF
PMISGIEEVRSAKAVLRECMEELRSAGIPFDEKIETGIMIEVPSAVTIADLLAREVDFFSVGTNDLIQYSLAIDRTNEHL
AHLYQPLHPAILRSLKTVADAAHAAGIRVCMCGEMAGEPLYLPILLGLGFDELSMTAVSIPRVKKILRRARRSDAERLVA
RALTFATAAEVESFVKSEISARFSESFD

Sequences:

>Translated_588_residues
MGSARSETRVFSGIGASPGIAIGTARVTDRSRVMVVEASVPPEDIPGEVVRFTAALSRSRDDLLALKESIAARRGAEHLY
VVDAHLLILDDSMLRQGTIDLIERDGVNAEAALKKNLDKFKEFFAGIDDDYLRERFGDVETVVERILRHMVGKRHERIDA
IDEGKRLIVAHDLSPTDILQMDKEKVVGFVTDLGGKTSHTSILARAFEIPAVVGLETITVEDIDGLPIIIDGTTGTIVVN
PSEETFRDYLRRKQHYDYLEQELLKLKDLPAVTLDGHSMRLMGNIEFPEEIPSLKGHGGQGVGLYRTEMLYMNRPGLPDE
EEQYRAYATIVENVAPEPVTIRTLDIGGDKLATDLHLEDEMNPAMGLRAIRLSLRRPEVFKSQLRAILRAGCKGSVRLFF
PMISGIEEVRSAKAVLRECMEELRSAGIPFDEKIETGIMIEVPSAVTIADLLAREVDFFSVGTNDLIQYSLAIDRTNEHL
AHLYQPLHPAILRSLKTVADAAHAAGIRVCMCGEMAGEPLYLPILLGLGFDELSMTAVSIPRVKKILRRARRSDAERLVA
RALTFATAAEVESFVKSEISARFSESFD
>Mature_587_residues
GSARSETRVFSGIGASPGIAIGTARVTDRSRVMVVEASVPPEDIPGEVVRFTAALSRSRDDLLALKESIAARRGAEHLYV
VDAHLLILDDSMLRQGTIDLIERDGVNAEAALKKNLDKFKEFFAGIDDDYLRERFGDVETVVERILRHMVGKRHERIDAI
DEGKRLIVAHDLSPTDILQMDKEKVVGFVTDLGGKTSHTSILARAFEIPAVVGLETITVEDIDGLPIIIDGTTGTIVVNP
SEETFRDYLRRKQHYDYLEQELLKLKDLPAVTLDGHSMRLMGNIEFPEEIPSLKGHGGQGVGLYRTEMLYMNRPGLPDEE
EQYRAYATIVENVAPEPVTIRTLDIGGDKLATDLHLEDEMNPAMGLRAIRLSLRRPEVFKSQLRAILRAGCKGSVRLFFP
MISGIEEVRSAKAVLRECMEELRSAGIPFDEKIETGIMIEVPSAVTIADLLAREVDFFSVGTNDLIQYSLAIDRTNEHLA
HLYQPLHPAILRSLKTVADAAHAAGIRVCMCGEMAGEPLYLPILLGLGFDELSMTAVSIPRVKKILRRARRSDAERLVAR
ALTFATAAEVESFVKSEISARFSESFD

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=574, Percent_Identity=41.6376306620209, Blast_Score=446, Evalue=1e-126,
Organism=Escherichia coli, GI48994992, Length=495, Percent_Identity=37.3737373737374, Blast_Score=318, Evalue=7e-88,
Organism=Escherichia coli, GI1789193, Length=586, Percent_Identity=36.0068259385666, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1788726, Length=504, Percent_Identity=35.1190476190476, Blast_Score=304, Evalue=1e-83,
Organism=Escherichia coli, GI1787994, Length=476, Percent_Identity=25.8403361344538, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI226510935, Length=129, Percent_Identity=26.3565891472868, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 65127; Mature: 64996

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2 ; PS00449 ATPASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSARSETRVFSGIGASPGIAIGTARVTDRSRVMVVEASVPPEDIPGEVVRFTAALSRSR
CCCCCCHHHHHHCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCH
DDLLALKESIAARRGAEHLYVVDAHLLILDDSMLRQGTIDLIERDGVNAEAALKKNLDKF
HHHHHHHHHHHHHCCCCEEEEEEEEEEEECCHHHHCCCHHHHHCCCCCHHHHHHHHHHHH
KEFFAGIDDDYLRERFGDVETVVERILRHMVGKRHERIDAIDEGKRLIVAHDLSPTDILQ
HHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHCCHHHHCCHHCCCCEEEEEECCCCHHHHH
MDKEKVVGFVTDLGGKTSHTSILARAFEIPAVVGLETITVEDIDGLPIIIDGTTGTIVVN
HCHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHCEEEEEEECCCCCEEEEECCCCEEEEC
PSEETFRDYLRRKQHYDYLEQELLKLKDLPAVTLDGHSMRLMGNIEFPEEIPSLKGHGGQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCHHHCCCCCCCCCC
GVGLYRTEMLYMNRPGLPDEEEQYRAYATIVENVAPEPVTIRTLDIGGDKLATDLHLEDE
CCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEECCC
MNPAMGLRAIRLSLRRPEVFKSQLRAILRAGCKGSVRLFFPMISGIEEVRSAKAVLRECM
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
EELRSAGIPFDEKIETGIMIEVPSAVTIADLLAREVDFFSVGTNDLIQYSLAIDRTNEHL
HHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCEEECCCHHHEEEEEEECCCHHHH
AHLYQPLHPAILRSLKTVADAAHAAGIRVCMCGEMAGEPLYLPILLGLGFDELSMTAVSI
HHHHHCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEHHHHCCCCHHHHHHHHH
PRVKKILRRARRSDAERLVARALTFATAAEVESFVKSEISARFSESFD
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GSARSETRVFSGIGASPGIAIGTARVTDRSRVMVVEASVPPEDIPGEVVRFTAALSRSR
CCCCCHHHHHHCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCH
DDLLALKESIAARRGAEHLYVVDAHLLILDDSMLRQGTIDLIERDGVNAEAALKKNLDKF
HHHHHHHHHHHHHCCCCEEEEEEEEEEEECCHHHHCCCHHHHHCCCCCHHHHHHHHHHHH
KEFFAGIDDDYLRERFGDVETVVERILRHMVGKRHERIDAIDEGKRLIVAHDLSPTDILQ
HHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHCCHHHHCCHHCCCCEEEEEECCCCHHHHH
MDKEKVVGFVTDLGGKTSHTSILARAFEIPAVVGLETITVEDIDGLPIIIDGTTGTIVVN
HCHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHCEEEEEEECCCCCEEEEECCCCEEEEC
PSEETFRDYLRRKQHYDYLEQELLKLKDLPAVTLDGHSMRLMGNIEFPEEIPSLKGHGGQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCHHHCCCCCCCCCC
GVGLYRTEMLYMNRPGLPDEEEQYRAYATIVENVAPEPVTIRTLDIGGDKLATDLHLEDE
CCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEECCC
MNPAMGLRAIRLSLRRPEVFKSQLRAILRAGCKGSVRLFFPMISGIEEVRSAKAVLRECM
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
EELRSAGIPFDEKIETGIMIEVPSAVTIADLLAREVDFFSVGTNDLIQYSLAIDRTNEHL
HHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCEEECCCHHHEEEEEEECCCHHHH
AHLYQPLHPAILRSLKTVADAAHAAGIRVCMCGEMAGEPLYLPILLGLGFDELSMTAVSI
HHHHHCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEHHHHCCCCHHHHHHHHH
PRVKKILRRARRSDAERLVARALTFATAAEVESFVKSEISARFSESFD
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]