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The map label for this gene is metK [H]

Identifier: 308513310

GI number: 308513310

Start: 2058779

End: 2059948

Strand: Reverse

Name: metK [H]

Synonym: NA

Alternate gene names: 308513310

Gene position: 2059948-2058779 (Counterclockwise)

Preceding gene: 39996979

Following gene: 39996975

Centisome position: 54.01

GC content: 59.74

Gene sequence:

>1170_bases
ATGGAGATGAAGGATTATATTTTCACGTCCGAATCGGTATCCGAAGGTCACCCCGACAAGGTTGCCGACCAGGTTTCGGA
TGCCATTCTCGATGCGATACTTACTCAGGACAAAAGGGCGAGGGTTGCCTGTGAAACGCTTGTTACCACCGGCATGGCGG
TAATCGCCGGCGAAATCACCACCACAGCGGTGGTCGACTACCCGAAGGTCGTGCGCGAGACCATCAAGGAGATCGGCTAC
AACGATTCGGCCATGGGCTTCGACTGGGAAACCTGCGCGGTCCTTGTCTCCATTGACAAGCAATCCCCCGACATCTCCCA
GGGCGTCACCGAGGGGGAAGGGATGTTCAAGGAGCAGGGCGCGGGCGACCAGGGGCTTATGTTCGGCTATGCCTGCAATG
AAACTCCCGAGCTTATGCCGATGACCATCATGTACTCGCACCGGCTGACCCAGAAGCTGGCCGAAGTCCGCAAAAACGGA
ACCCTCGATTTCCTTCGCCCCGACTCCAAATCGCAGGTTTCCATCCAGTATGTCGACGACAGGCCGGTCCGGGTCGACAC
GGTGGTCATCTCCTCCCAGCACACGCCGGAAGTCTCCTATGAAACCATCAAGGAAGGGATCATCGAGGAGGTCGTCAAGA
AGATCATCCCCGTGGAGCTCATGGACGACAAGACCCGCTTCCTCATCAACCCCACCGGCCGGTTCGTGATCGGTGGTCCC
ATGGGCGACTGCGGCCTCACGGGTCGCAAGATCATCGTCGACAGCTACGGCGGTCACGGTGCCCACGGCGGCGGCGCCTT
CTCGGGTAAGGATCCTTCCAAAGTCGACCGGTCAGCTGCCTACATGGGGCGTTACGTGGCCAAGAACCTGGTTGCGGCAG
GGCTGTGCGAGCGCTGCGAAGTTCAGGTCGCCTATGCCATCGGCGTGGCCGAGCCCGTGTCGGTCATGGTCGACACTGCG
GGTACCGGCAAAATTCCGTCCGCACGAATCGCGGAGATCATCCGTGAGGTTTTTGATCTGCGTCCCCGCGCCATCATCGA
GCAGCTCGACCTGCTTCGCCCGATTTACCGCAAGACCGCGGCCTACGGCCATTTCGGCCGCGAACTCCCCGAATTCACCT
GGGAGCGGACCGACAAGATCGATATCATCCGCCAGAAGGCTGGAATCTAA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:
NA

Sequences:

>Translated_389_residues
MEMKDYIFTSESVSEGHPDKVADQVSDAILDAILTQDKRARVACETLVTTGMAVIAGEITTTAVVDYPKVVRETIKEIGY
NDSAMGFDWETCAVLVSIDKQSPDISQGVTEGEGMFKEQGAGDQGLMFGYACNETPELMPMTIMYSHRLTQKLAEVRKNG
TLDFLRPDSKSQVSIQYVDDRPVRVDTVVISSQHTPEVSYETIKEGIIEEVVKKIIPVELMDDKTRFLINPTGRFVIGGP
MGDCGLTGRKIIVDSYGGHGAHGGGAFSGKDPSKVDRSAAYMGRYVAKNLVAAGLCERCEVQVAYAIGVAEPVSVMVDTA
GTGKIPSARIAEIIREVFDLRPRAIIEQLDLLRPIYRKTAAYGHFGRELPEFTWERTDKIDIIRQKAGI
>Mature_389_residues
MEMKDYIFTSESVSEGHPDKVADQVSDAILDAILTQDKRARVACETLVTTGMAVIAGEITTTAVVDYPKVVRETIKEIGY
NDSAMGFDWETCAVLVSIDKQSPDISQGVTEGEGMFKEQGAGDQGLMFGYACNETPELMPMTIMYSHRLTQKLAEVRKNG
TLDFLRPDSKSQVSIQYVDDRPVRVDTVVISSQHTPEVSYETIKEGIIEEVVKKIIPVELMDDKTRFLINPTGRFVIGGP
MGDCGLTGRKIIVDSYGGHGAHGGGAFSGKDPSKVDRSAAYMGRYVAKNLVAAGLCERCEVQVAYAIGVAEPVSVMVDTA
GTGKIPSARIAEIIREVFDLRPRAIIEQLDLLRPIYRKTAAYGHFGRELPEFTWERTDKIDIIRQKAGI

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=384, Percent_Identity=57.5520833333333, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI4557737, Length=379, Percent_Identity=56.2005277044855, Blast_Score=426, Evalue=1e-119,
Organism=Escherichia coli, GI1789311, Length=387, Percent_Identity=64.5994832041344, Blast_Score=531, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI17538494, Length=386, Percent_Identity=57.2538860103627, Blast_Score=439, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17538498, Length=386, Percent_Identity=57.5129533678757, Blast_Score=439, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI25145633, Length=386, Percent_Identity=57.5129533678757, Blast_Score=437, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17551082, Length=384, Percent_Identity=55.9895833333333, Blast_Score=425, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI32565905, Length=386, Percent_Identity=51.0362694300518, Blast_Score=371, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI32565911, Length=335, Percent_Identity=56.4179104477612, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI25145635, Length=335, Percent_Identity=56.4179104477612, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI32565903, Length=308, Percent_Identity=55.5194805194805, Blast_Score=332, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI32565909, Length=87, Percent_Identity=60.9195402298851, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32565907, Length=44, Percent_Identity=65.9090909090909, Blast_Score=68, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6320710, Length=383, Percent_Identity=56.9190600522193, Blast_Score=445, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6323209, Length=375, Percent_Identity=57.6, Blast_Score=443, Evalue=1e-125,
Organism=Drosophila melanogaster, GI45552159, Length=381, Percent_Identity=56.4304461942257, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580529, Length=381, Percent_Identity=56.4304461942257, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580537, Length=381, Percent_Identity=56.4304461942257, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580533, Length=381, Percent_Identity=56.4304461942257, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580535, Length=381, Percent_Identity=56.4304461942257, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580531, Length=381, Percent_Identity=56.4304461942257, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580527, Length=384, Percent_Identity=55.9895833333333, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580525, Length=384, Percent_Identity=55.9895833333333, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580541, Length=368, Percent_Identity=55.4347826086957, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580539, Length=122, Percent_Identity=59.8360655737705, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42697; Mature: 42697

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMKDYIFTSESVSEGHPDKVADQVSDAILDAILTQDKRARVACETLVTTGMAVIAGEIT
CCCCHHEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHCCCHH
TTAVVDYPKVVRETIKEIGYNDSAMGFDWETCAVLVSIDKQSPDISQGVTEGEGMFKEQG
HHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHEEEEEEECCCCCCHHHCCCCCCCCHHHCC
AGDQGLMFGYACNETPELMPMTIMYSHRLTQKLAEVRKNGTLDFLRPDSKSQVSIQYVDD
CCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEECC
RPVRVDTVVISSQHTPEVSYETIKEGIIEEVVKKIIPVELMDDKTRFLINPTGRFVIGGP
CCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCEEEECCCCCEEECCC
MGDCGLTGRKIIVDSYGGHGAHGGGAFSGKDPSKVDRSAAYMGRYVAKNLVAAGLCERCE
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VQVAYAIGVAEPVSVMVDTAGTGKIPSARIAEIIREVFDLRPRAIIEQLDLLRPIYRKTA
EEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AYGHFGRELPEFTWERTDKIDIIRQKAGI
HHHHHHHHHHHHHHCCCCHHHHHHHHCCC
>Mature Secondary Structure
MEMKDYIFTSESVSEGHPDKVADQVSDAILDAILTQDKRARVACETLVTTGMAVIAGEIT
CCCCHHEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHCCCHH
TTAVVDYPKVVRETIKEIGYNDSAMGFDWETCAVLVSIDKQSPDISQGVTEGEGMFKEQG
HHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHEEEEEEECCCCCCHHHCCCCCCCCHHHCC
AGDQGLMFGYACNETPELMPMTIMYSHRLTQKLAEVRKNGTLDFLRPDSKSQVSIQYVDD
CCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEECC
RPVRVDTVVISSQHTPEVSYETIKEGIIEEVVKKIIPVELMDDKTRFLINPTGRFVIGGP
CCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCEEEECCCCCEEECCC
MGDCGLTGRKIIVDSYGGHGAHGGGAFSGKDPSKVDRSAAYMGRYVAKNLVAAGLCERCE
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VQVAYAIGVAEPVSVMVDTAGTGKIPSARIAEIIREVFDLRPRAIIEQLDLLRPIYRKTA
EEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AYGHFGRELPEFTWERTDKIDIIRQKAGI
HHHHHHHHHHHHHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA