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The map label for this gene is outE [H]

Identifier: 39996882

GI number: 39996882

Start: 1945917

End: 1947641

Strand: Reverse

Name: outE [H]

Synonym: GSU1783

Alternate gene names: 39996882

Gene position: 1947641-1945917 (Counterclockwise)

Preceding gene: 39996883

Following gene: 39996881

Centisome position: 51.06

GC content: 55.88

Gene sequence:

>1725_bases
ATGGAAAAGCAATCTTTCAAACGAAAAACCATCGGCCAGATCCTTGTTCAACAGGGAAGCCTCAATCCTGATCAGATCCC
CTATCTGGTCGAGAAGAGAAATGCGTCCACAAAGCGTTTCGGGGAGGTCTGCGTTGGGGACGGACTCATTACCGAGGAGA
ACCTGGCACGGGCTCTTGCCGAGCAGTTCGGCCTCGACTATGTTGATGCCCGCGGCGTGCGGCTCAATGAGTCGCTCCTG
GCTACACTGCCACCGGATGCGATCTATCGCTACCAGTTCGTCCCGTTGGAAGAGGAAGACGGGACCCTGGTCGTTCTTAT
TGCCGATCCGACAGATGTTCTGAAGCTGGACGAGTTGGAGCTTCTGCTGGACCGGCCTTTTATCGTCAAACTGGCGACGG
AAACGGCCATTGCCACTATCCTCAAGAAAGGGGAGGCCACCAGTAGGGTCCTCAAGGAAGTTTCCGAGGACTTCATGCTG
CAACTGGTCAAGGAGAATGAAAAAGGCGAAGAGATACTCTCCATGGAGAAGATCTCGGCCGACACGAGCCCCATCATCAA
GCTCGTCAACTCAACCGTCATGGACGCGCTGACCCGTCGGGCAAGCGATATTCACATCGAAACCGCCCTGGAAGGGGTTA
TCATCAAATACCGGATCGACGGGGTGCTATACCGGGCCACCGAACCCCTCGATATTCACTTTCAGGCTCCTATCATTTCT
CGCCTCAAGGTCATGAGTGAGCTCGACATTTCCGAGCGACGCATCCCTCAGGACGGTCGCTTCAAGGTCAGGCTCAATGA
CAAGGCCATTGATTTTCGCGTATCGATCATGCCGAGCGCCTTTGGAGAGGACGCGGTTATCCGTATCCTTGACAAGGAGA
GCATCGCTTCCGACCTGAAGGGGCTTACGCTTGAGACGCTCGGCATGCATCCGCGGGAAATGAAGCGTCTGAGAAGGAAA
ATTCGCGAGCCTTACGGCATGGTCCTCGTAACGGGGCCGACCGGTTCGGGCAAGACGACTACCCTCTACGCCGCTCTCAC
CGAGATTCATACGGGCGAAGAGAAGATCATTACCATTGAAGACCCGGTCGAATACGTGTTGCGCGGTATCGTCCAGATAC
CGGTGAACGAGAAAAAGGGCCTCACCTTTGCCCGCGGTCTCCGGTCGATTCTTCGGCACGACCCGGACAAGATCATGGTC
GGCGAGATCCGGGACCCTGAAACGGCACAAATCGCCGTCCAATCGGCCCTCACCGGGCACTTGGTCTTTACGACGGTTCA
CGCGAACAACGCTTTTGACGTTCTTGGCCGCTTCATTCACATGGGGATCGATCCCTACAACTTCGTTTCGTGTCTCAACT
GTGTCATGGCCCAGCGTCTGGTACGCAAGGCGTGTCCGCACTGCAAATATCCGGTGGAGCATTCGGATTCGGTCCTTATC
GAATCGGGACTTGACCCGGAGGAGTGTCGCGACGTAACGTTCTATGAGAGTCGTGGCTGCGAGGAGTGCAATGGGACGGG
ATATCGCGGACGATCAGCCATCATTGAACTTCTTGATCTGAATGACCAGATGCGGGAGCTGATCATGGCGAAGGCTCCTG
CAGCCCAGCTCAAGGCGGCCGCCCGTGAGTCCGGCACGGTATTCCTGCGCGAATCTGCCATTGAAAAGGTCTTTGCAGGG
GAAACGACCTTGCGTGAAATCAACCGGGTCACCTTCGTGGAGTGA

Upstream 100 bases:

>100_bases
TATTATCGTAACCATGTACCTGCCGGTGTTCAAAATTGCCGGCACTGTAGGATGATTGTCGCGGCGCAGCCCATTCAACG
GCCCGGCTTGGAACGAAGCA

Downstream 100 bases:

>100_bases
GCATGCTTTTCGCCCAGAACGCCATAGGTATGGAAGTGTCGCAGCACGGGGTCCGCTTTGTCGTGCTGGGTGGCGGCAAG
GGTGCGCCGCGTCTCGTGAC

Product: type IV pilus biogenesis protein PilB

Products: NA

Alternate protein names: Pectic enzymes secretion protein outE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MEKQSFKRKTIGQILVQQGSLNPDQIPYLVEKRNASTKRFGEVCVGDGLITEENLARALAEQFGLDYVDARGVRLNESLL
ATLPPDAIYRYQFVPLEEEDGTLVVLIADPTDVLKLDELELLLDRPFIVKLATETAIATILKKGEATSRVLKEVSEDFML
QLVKENEKGEEILSMEKISADTSPIIKLVNSTVMDALTRRASDIHIETALEGVIIKYRIDGVLYRATEPLDIHFQAPIIS
RLKVMSELDISERRIPQDGRFKVRLNDKAIDFRVSIMPSAFGEDAVIRILDKESIASDLKGLTLETLGMHPREMKRLRRK
IREPYGMVLVTGPTGSGKTTTLYAALTEIHTGEEKIITIEDPVEYVLRGIVQIPVNEKKGLTFARGLRSILRHDPDKIMV
GEIRDPETAQIAVQSALTGHLVFTTVHANNAFDVLGRFIHMGIDPYNFVSCLNCVMAQRLVRKACPHCKYPVEHSDSVLI
ESGLDPEECRDVTFYESRGCEECNGTGYRGRSAIIELLDLNDQMRELIMAKAPAAQLKAAARESGTVFLRESAIEKVFAG
ETTLREINRVTFVE

Sequences:

>Translated_574_residues
MEKQSFKRKTIGQILVQQGSLNPDQIPYLVEKRNASTKRFGEVCVGDGLITEENLARALAEQFGLDYVDARGVRLNESLL
ATLPPDAIYRYQFVPLEEEDGTLVVLIADPTDVLKLDELELLLDRPFIVKLATETAIATILKKGEATSRVLKEVSEDFML
QLVKENEKGEEILSMEKISADTSPIIKLVNSTVMDALTRRASDIHIETALEGVIIKYRIDGVLYRATEPLDIHFQAPIIS
RLKVMSELDISERRIPQDGRFKVRLNDKAIDFRVSIMPSAFGEDAVIRILDKESIASDLKGLTLETLGMHPREMKRLRRK
IREPYGMVLVTGPTGSGKTTTLYAALTEIHTGEEKIITIEDPVEYVLRGIVQIPVNEKKGLTFARGLRSILRHDPDKIMV
GEIRDPETAQIAVQSALTGHLVFTTVHANNAFDVLGRFIHMGIDPYNFVSCLNCVMAQRLVRKACPHCKYPVEHSDSVLI
ESGLDPEECRDVTFYESRGCEECNGTGYRGRSAIIELLDLNDQMRELIMAKAPAAQLKAAARESGTVFLRESAIEKVFAG
ETTLREINRVTFVE
>Mature_574_residues
MEKQSFKRKTIGQILVQQGSLNPDQIPYLVEKRNASTKRFGEVCVGDGLITEENLARALAEQFGLDYVDARGVRLNESLL
ATLPPDAIYRYQFVPLEEEDGTLVVLIADPTDVLKLDELELLLDRPFIVKLATETAIATILKKGEATSRVLKEVSEDFML
QLVKENEKGEEILSMEKISADTSPIIKLVNSTVMDALTRRASDIHIETALEGVIIKYRIDGVLYRATEPLDIHFQAPIIS
RLKVMSELDISERRIPQDGRFKVRLNDKAIDFRVSIMPSAFGEDAVIRILDKESIASDLKGLTLETLGMHPREMKRLRRK
IREPYGMVLVTGPTGSGKTTTLYAALTEIHTGEEKIITIEDPVEYVLRGIVQIPVNEKKGLTFARGLRSILRHDPDKIMV
GEIRDPETAQIAVQSALTGHLVFTTVHANNAFDVLGRFIHMGIDPYNFVSCLNCVMAQRLVRKACPHCKYPVEHSDSVLI
ESGLDPEECRDVTFYESRGCEECNGTGYRGRSAIIELLDLNDQMRELIMAKAPAAQLKAAARESGTVFLRESAIEKVFAG
ETTLREINRVTFVE

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pectate lyases and cellulases across the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=426, Percent_Identity=41.5492957746479, Blast_Score=327, Evalue=1e-90,
Organism=Escherichia coli, GI1786296, Length=390, Percent_Identity=41.7948717948718, Blast_Score=292, Evalue=4e-80,
Organism=Escherichia coli, GI87082188, Length=280, Percent_Identity=32.8571428571429, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 64240; Mature: 64240

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00284 SERPIN ; PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKQSFKRKTIGQILVQQGSLNPDQIPYLVEKRNASTKRFGEVCVGDGLITEENLARALA
CCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHH
EQFGLDYVDARGVRLNESLLATLPPDAIYRYQFVPLEEEDGTLVVLIADPTDVLKLDELE
HHHCCCEECCCCCEECCCCEEECCCCHHEEEEEEEEECCCCEEEEEEECCCCHHHHHHHH
LLLDRPFIVKLATETAIATILKKGEATSRVLKEVSEDFMLQLVKENEKGEEILSMEKISA
HHHCCCEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
DTSPIIKLVNSTVMDALTRRASDIHIETALEGVIIKYRIDGVLYRATEPLDIHFQAPIIS
CHHHHHHHHHHHHHHHHHHHHCCEEEEEHHCCEEEEEEECEEEEECCCCEEEEECCHHHH
RLKVMSELDISERRIPQDGRFKVRLNDKAIDFRVSIMPSAFGEDAVIRILDKESIASDLK
HHHHHHHCCCHHHCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCEEEEECHHHHHHHHC
GLTLETLGMHPREMKRLRRKIREPYGMVLVTGPTGSGKTTTLYAALTEIHTGEEKIITIE
CCCHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEHEEEHHHHCCCCCEEEEEC
DPVEYVLRGIVQIPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDPETAQIAVQSALTGH
CHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCE
LVFTTVHANNAFDVLGRFIHMGIDPYNFVSCLNCVMAQRLVRKACPHCKYPVEHSDSVLI
EEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
ESGLDPEECRDVTFYESRGCEECNGTGYRGRSAIIELLDLNDQMRELIMAKAPAAQLKAA
ECCCCHHHHCCCCEECCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCHHHHHHH
ARESGTVFLRESAIEKVFAGETTLREINRVTFVE
HHCCCEEEEEHHHHHHHHCCCHHHHHHCHHCCCC
>Mature Secondary Structure
MEKQSFKRKTIGQILVQQGSLNPDQIPYLVEKRNASTKRFGEVCVGDGLITEENLARALA
CCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHH
EQFGLDYVDARGVRLNESLLATLPPDAIYRYQFVPLEEEDGTLVVLIADPTDVLKLDELE
HHHCCCEECCCCCEECCCCEEECCCCHHEEEEEEEEECCCCEEEEEEECCCCHHHHHHHH
LLLDRPFIVKLATETAIATILKKGEATSRVLKEVSEDFMLQLVKENEKGEEILSMEKISA
HHHCCCEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
DTSPIIKLVNSTVMDALTRRASDIHIETALEGVIIKYRIDGVLYRATEPLDIHFQAPIIS
CHHHHHHHHHHHHHHHHHHHHCCEEEEEHHCCEEEEEEECEEEEECCCCEEEEECCHHHH
RLKVMSELDISERRIPQDGRFKVRLNDKAIDFRVSIMPSAFGEDAVIRILDKESIASDLK
HHHHHHHCCCHHHCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCEEEEECHHHHHHHHC
GLTLETLGMHPREMKRLRRKIREPYGMVLVTGPTGSGKTTTLYAALTEIHTGEEKIITIE
CCCHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEHEEEHHHHCCCCCEEEEEC
DPVEYVLRGIVQIPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDPETAQIAVQSALTGH
CHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCE
LVFTTVHANNAFDVLGRFIHMGIDPYNFVSCLNCVMAQRLVRKACPHCKYPVEHSDSVLI
EEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
ESGLDPEECRDVTFYESRGCEECNGTGYRGRSAIIELLDLNDQMRELIMAKAPAAQLKAA
ECCCCHHHHCCCCEECCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCHHHHHHH
ARESGTVFLRESAIEKVFAGETTLREINRVTFVE
HHCCCEEEEEHHHHHHHHCCCHHHHHHCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1429461 [H]