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The map label for this gene is purF [H]

Identifier: 39996736

GI number: 39996736

Start: 1795135

End: 1796538

Strand: Direct

Name: purF [H]

Synonym: GSU1636

Alternate gene names: 39996736

Gene position: 1795135-1796538 (Clockwise)

Preceding gene: 39996735

Following gene: 39996737

Centisome position: 47.07

GC content: 59.54

Gene sequence:

>1404_bases
ATGATGAAGCTCTACAGACCAACGGAAGAGTGCGGCATTTTCGGTATCTACGGCCACCCTGAGGCGGCCAATCTGACGTA
CCTGGGCCTTTACGCCCTTCAGCACCGGGGACAGGAAGCCTGCGGCATCGTCTCCTCGGACGGCCGCAGCCTCTACACCC
ACCGGAGCATGGGGCTTGTGGCGGATGTCTTCGGCAATCAGGAAATCTTCGGCAATCTTCCCGGTGAATCCGCCATCGGG
CACGTGCGCTACTCCACCACCGGCGACTCGGTCCTCAAGAACGTGCAGCCCATCAAGGTCGACTACTCCAGGGGCTCCAT
TGCCATTGCCCACAACGGCAACCTGGTGAACGCCCAGTACATCAAGGACGAGTTGGAGGCCTGGGGTTCCATTTTCCAGA
CCACTATGGACACGGAGGTGATCCTCCACCTGCTGGCCACTTCCAAGCATAGTTCCCTGGAGGACCGGATCATCGATGCC
CTCGGGCGGATCAAGGGGGCCTACTGTCTTCTGTTCCTGACGGAAACCCGCATGGTGGCTGCCCGCGATCCCCACGGCTT
CCGCCCCCTCTGTCTCGGCAAGCTCGGCGATGCCTGGGTGGTTGCGTCCGAGAGCTGTGCCTTGGACCTGATCGAGGCCG
AATTTGTCAGGGAGATCGAGCCGGGCGAGATCGTGGTCATTACCAAGGACGGGCTCACCTCCCATTTTCCGCATAAGAAG
ATCGAACCGGCTCCTTGTATTTTCGAGTTCGTCTACTTCGCTCGCCCAGATTCGTACATCTTCGGCAAGAACGTCTACCA
GGTACGCAAGGACTTCGGGCGCCAACTCTGCCGCGAGCACCGCATCGATGCCGATATCGTCATCCCGGTTCCCGATTCGG
GTGTTCCCGCAGCCCTGGGTTATGCCCAGGAGGCGGGTATTCCCTTTGAGCTCGGGCTTATCCGCAACCATTACGTGGGC
CGTACCTTCATTGAGCCCCAGCAGTCGATCCGGCATTTTGGTGTCAAGATCAAGCTCAATCCGGTGCGGGAGGTGCTGAA
GGGAAAGCGGGTGGTGGTCATCGACGACTCTATCGTCAGAGGCACCACGTCCCGCAAGATCGTGAAAATGGTACGCAACG
CCGGCGCATCCGAGGTTCACGTTCGCATCTCGTCGCCCCCCACGAGCTATCCATGCTACTACGGCATTGACACCCCCACC
CGCAAAGAACTTATCTCCTCGTCCCATACCATCGAGGAAATCCGCAAGTACATAACCGCCGATTCACTGGGCTACCTTTC
GGAGGAAGGGCTGCTCCAGGCCGTGGGCGCCGGCAGCAATCCCTTCTGCAAAGCCTGTTTCTCCGGCGGCTACCCGATCA
CATTCCCGCGCCTCATGGCCGAACCCCAACTGGATCTTTTCTGA

Upstream 100 bases:

>100_bases
CGAAGAGGGAGATGGACTCGTCCTGTTCCGCAACGCGGCCGACTACGCGAAGGCAAACCTGCTGTAGGGGGCGGTTTCCC
GCCCACACCATTTTGGAGAG

Downstream 100 bases:

>100_bases
AAGGAGTGAACCGACCAATGACTGACAAGGACCGACTGAAGCAGATCATCCTGGAGCTGTCCTACGAAAAGCGGAAAGTA
ATCCTCGCTTCGGGCCGCGA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MMKLYRPTEECGIFGIYGHPEAANLTYLGLYALQHRGQEACGIVSSDGRSLYTHRSMGLVADVFGNQEIFGNLPGESAIG
HVRYSTTGDSVLKNVQPIKVDYSRGSIAIAHNGNLVNAQYIKDELEAWGSIFQTTMDTEVILHLLATSKHSSLEDRIIDA
LGRIKGAYCLLFLTETRMVAARDPHGFRPLCLGKLGDAWVVASESCALDLIEAEFVREIEPGEIVVITKDGLTSHFPHKK
IEPAPCIFEFVYFARPDSYIFGKNVYQVRKDFGRQLCREHRIDADIVIPVPDSGVPAALGYAQEAGIPFELGLIRNHYVG
RTFIEPQQSIRHFGVKIKLNPVREVLKGKRVVVIDDSIVRGTTSRKIVKMVRNAGASEVHVRISSPPTSYPCYYGIDTPT
RKELISSSHTIEEIRKYITADSLGYLSEEGLLQAVGAGSNPFCKACFSGGYPITFPRLMAEPQLDLF

Sequences:

>Translated_467_residues
MMKLYRPTEECGIFGIYGHPEAANLTYLGLYALQHRGQEACGIVSSDGRSLYTHRSMGLVADVFGNQEIFGNLPGESAIG
HVRYSTTGDSVLKNVQPIKVDYSRGSIAIAHNGNLVNAQYIKDELEAWGSIFQTTMDTEVILHLLATSKHSSLEDRIIDA
LGRIKGAYCLLFLTETRMVAARDPHGFRPLCLGKLGDAWVVASESCALDLIEAEFVREIEPGEIVVITKDGLTSHFPHKK
IEPAPCIFEFVYFARPDSYIFGKNVYQVRKDFGRQLCREHRIDADIVIPVPDSGVPAALGYAQEAGIPFELGLIRNHYVG
RTFIEPQQSIRHFGVKIKLNPVREVLKGKRVVVIDDSIVRGTTSRKIVKMVRNAGASEVHVRISSPPTSYPCYYGIDTPT
RKELISSSHTIEEIRKYITADSLGYLSEEGLLQAVGAGSNPFCKACFSGGYPITFPRLMAEPQLDLF
>Mature_467_residues
MMKLYRPTEECGIFGIYGHPEAANLTYLGLYALQHRGQEACGIVSSDGRSLYTHRSMGLVADVFGNQEIFGNLPGESAIG
HVRYSTTGDSVLKNVQPIKVDYSRGSIAIAHNGNLVNAQYIKDELEAWGSIFQTTMDTEVILHLLATSKHSSLEDRIIDA
LGRIKGAYCLLFLTETRMVAARDPHGFRPLCLGKLGDAWVVASESCALDLIEAEFVREIEPGEIVVITKDGLTSHFPHKK
IEPAPCIFEFVYFARPDSYIFGKNVYQVRKDFGRQLCREHRIDADIVIPVPDSGVPAALGYAQEAGIPFELGLIRNHYVG
RTFIEPQQSIRHFGVKIKLNPVREVLKGKRVVVIDDSIVRGTTSRKIVKMVRNAGASEVHVRISSPPTSYPCYYGIDTPT
RKELISSSHTIEEIRKYITADSLGYLSEEGLLQAVGAGSNPFCKACFSGGYPITFPRLMAEPQLDLF

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=507, Percent_Identity=40.6311637080868, Blast_Score=354, Evalue=1e-97,
Organism=Escherichia coli, GI1788651, Length=469, Percent_Identity=40.9381663113006, Blast_Score=305, Evalue=6e-84,
Organism=Escherichia coli, GI1790167, Length=225, Percent_Identity=28.4444444444444, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17554892, Length=479, Percent_Identity=41.9624217118998, Blast_Score=338, Evalue=4e-93,
Organism=Saccharomyces cerevisiae, GI6323958, Length=472, Percent_Identity=36.228813559322, Blast_Score=251, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24659598, Length=495, Percent_Identity=42.4242424242424, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI28573187, Length=493, Percent_Identity=43.0020283975659, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24659604, Length=469, Percent_Identity=43.2835820895522, Blast_Score=364, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 51716; Mature: 51716

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKLYRPTEECGIFGIYGHPEAANLTYLGLYALQHRGQEACGIVSSDGRSLYTHRSMGLV
CCCCCCCHHHCCEEEEECCCCCCCEEEHHHHHHHHCCCHHCCEEECCCCEEEEECCCCHH
ADVFGNQEIFGNLPGESAIGHVRYSTTGDSVLKNVQPIKVDYSRGSIAIAHNGNLVNAQY
HHHCCCHHEECCCCCCCCCEEEEECCCHHHHHCCCCCEEEEECCCCEEEEECCCEEEHHH
IKDELEAWGSIFQTTMDTEVILHLLATSKHSSLEDRIIDALGRIKGAYCLLFLTETRMVA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEEEECCEEEE
ARDPHGFRPLCLGKLGDAWVVASESCALDLIEAEFVREIEPGEIVVITKDGLTSHFPHKK
ECCCCCCCCHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCC
IEPAPCIFEFVYFARPDSYIFGKNVYQVRKDFGRQLCREHRIDADIVIPVPDSGVPAALG
CCCCHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHC
YAQEAGIPFELGLIRNHYVGRTFIEPQQSIRHFGVKIKLNPVREVLKGKRVVVIDDSIVR
CHHHCCCCEEEEEHHCCCCCCCCCCHHHHHHHCCEEEEECHHHHHHCCCEEEEECCCHHC
GTTSRKIVKMVRNAGASEVHVRISSPPTSYPCYYGIDTPTRKELISSSHTIEEIRKYITA
CCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCHHHHHHHCCCHHHHHHHHHHH
DSLGYLSEEGLLQAVGAGSNPFCKACFSGGYPITFPRLMAEPQLDLF
CCCCCCCCCCHHHCCCCCCCHHHHHHHCCCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure
MMKLYRPTEECGIFGIYGHPEAANLTYLGLYALQHRGQEACGIVSSDGRSLYTHRSMGLV
CCCCCCCHHHCCEEEEECCCCCCCEEEHHHHHHHHCCCHHCCEEECCCCEEEEECCCCHH
ADVFGNQEIFGNLPGESAIGHVRYSTTGDSVLKNVQPIKVDYSRGSIAIAHNGNLVNAQY
HHHCCCHHEECCCCCCCCCEEEEECCCHHHHHCCCCCEEEEECCCCEEEEECCCEEEHHH
IKDELEAWGSIFQTTMDTEVILHLLATSKHSSLEDRIIDALGRIKGAYCLLFLTETRMVA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEEEECCEEEE
ARDPHGFRPLCLGKLGDAWVVASESCALDLIEAEFVREIEPGEIVVITKDGLTSHFPHKK
ECCCCCCCCHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCC
IEPAPCIFEFVYFARPDSYIFGKNVYQVRKDFGRQLCREHRIDADIVIPVPDSGVPAALG
CCCCHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHC
YAQEAGIPFELGLIRNHYVGRTFIEPQQSIRHFGVKIKLNPVREVLKGKRVVVIDDSIVR
CHHHCCCCEEEEEHHCCCCCCCCCCHHHHHHHCCEEEEECHHHHHHCCCEEEEECCCHHC
GTTSRKIVKMVRNAGASEVHVRISSPPTSYPCYYGIDTPTRKELISSSHTIEEIRKYITA
CCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCHHHHHHHCCCHHHHHHHHHHH
DSLGYLSEEGLLQAVGAGSNPFCKACFSGGYPITFPRLMAEPQLDLF
CCCCCCCCCCHHHCCCCCCCHHHHHHHCCCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]