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The map label for this gene is truB

Identifier: 39996691

GI number: 39996691

Start: 1744474

End: 1745388

Strand: Direct

Name: truB

Synonym: GSU1591

Alternate gene names: 39996691

Gene position: 1744474-1745388 (Clockwise)

Preceding gene: 39996690

Following gene: 39996692

Centisome position: 45.74

GC content: 58.47

Gene sequence:

>915_bases
ATGGACGGCTTTATCGTCATCGACAAACCGATCGGTCTCACCTCCCATGATGTGGTTGCGAGAGTCAGGCAAACCCTTCG
GCAGAAGAAGGCCGGTCACACCGGCACGCTTGATCCATTCGCCACCGGCGTTCTACCGGTTGCTGTCGGCGAGGGGACAA
AAGCGATTCCCTATCTGGATGAATCAACCAAAGTATACCGTGCCACGCTGATCCTTGGCGCTGCCACTGATACCCAGGAC
CACACCGGTCAGGTTGTTCATGCCGGCGACTGGCGGCACCTGGAGCCTCAGTCAGTTCGTGATGTCGTTTCCTCGTTCAC
GGGCAAACTCAGCCAGCTGCCCCCCATGTTTTCGGCCCTCAAGCGTGATGGCGTCCCCCTTTACAAACTCGCCCGCACTG
GCAGGGAAGTCGAGCGCGAACGCCGTGAGATAGAGGTTTTCTCATTGGTTGTCGACTCGATTGATCTGCCGTTGGTGACG
TTTACCCTGTCCTGCTCGCGCGGGACCTATGTCCGGACACTGGCCCACGACATGGGTGAGCGTCTTGGCTGCGGTGCTCA
CCTGACGGAGTTGCGCCGGTTGAGCAGCGGCCCCTTCAATCTGGACCGGGCTATATCCCTTGAACGGCTTAAAGAGCTGG
CAGAAGCCGGCAACCTTGCCGATGTCCTTGTGTCGCCATCCGACGCCCTGGGTCATCTTCATGCATTGCCGCTCACCGCC
CAGGGAGCTGAACGTGTCAGGCGCGGCATGCCCCCTTGCCGTGAGGATGTTGAGCATGGGGCGGGTACGGGAATACCGGC
GGGGACACGTCTGCGTTTGCTGAGAGACGGAATTGTTGTTGCTGTGGCGGATTCTCTTGCCGGTCTTTGGTCCAGTGATA
CCAAAAACCTAAGACTTTTAAGGGTGTTTAACTAG

Upstream 100 bases:

>100_bases
GCGGCGGGCACCACAATGCGGCCGGTTGTACCCTGACCGGTGATTTGGCCGAAGTCAGAAAGCAGGTTTTCGACCATCTG
GAGACTGCTCTCTGATTTTC

Downstream 100 bases:

>100_bases
GTTTTCCCCTTTACACGGCGCACCCTTTGTGGTACAAACACACTTTGCTGATCGTTCTGGCGCAGCAGTGCGCCGCCATG
CATCACATACTCATAATCCC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MDGFIVIDKPIGLTSHDVVARVRQTLRQKKAGHTGTLDPFATGVLPVAVGEGTKAIPYLDESTKVYRATLILGAATDTQD
HTGQVVHAGDWRHLEPQSVRDVVSSFTGKLSQLPPMFSALKRDGVPLYKLARTGREVERERREIEVFSLVVDSIDLPLVT
FTLSCSRGTYVRTLAHDMGERLGCGAHLTELRRLSSGPFNLDRAISLERLKELAEAGNLADVLVSPSDALGHLHALPLTA
QGAERVRRGMPPCREDVEHGAGTGIPAGTRLRLLRDGIVVAVADSLAGLWSSDTKNLRLLRVFN

Sequences:

>Translated_304_residues
MDGFIVIDKPIGLTSHDVVARVRQTLRQKKAGHTGTLDPFATGVLPVAVGEGTKAIPYLDESTKVYRATLILGAATDTQD
HTGQVVHAGDWRHLEPQSVRDVVSSFTGKLSQLPPMFSALKRDGVPLYKLARTGREVERERREIEVFSLVVDSIDLPLVT
FTLSCSRGTYVRTLAHDMGERLGCGAHLTELRRLSSGPFNLDRAISLERLKELAEAGNLADVLVSPSDALGHLHALPLTA
QGAERVRRGMPPCREDVEHGAGTGIPAGTRLRLLRDGIVVAVADSLAGLWSSDTKNLRLLRVFN
>Mature_304_residues
MDGFIVIDKPIGLTSHDVVARVRQTLRQKKAGHTGTLDPFATGVLPVAVGEGTKAIPYLDESTKVYRATLILGAATDTQD
HTGQVVHAGDWRHLEPQSVRDVVSSFTGKLSQLPPMFSALKRDGVPLYKLARTGREVERERREIEVFSLVVDSIDLPLVT
FTLSCSRGTYVRTLAHDMGERLGCGAHLTELRRLSSGPFNLDRAISLERLKELAEAGNLADVLVSPSDALGHLHALPLTA
QGAERVRRGMPPCREDVEHGAGTGIPAGTRLRLLRDGIVVAVADSLAGLWSSDTKNLRLLRVFN

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=220, Percent_Identity=35, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI215599015, Length=195, Percent_Identity=34.3589743589744, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI4503337, Length=195, Percent_Identity=34.3589743589744, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI2367200, Length=251, Percent_Identity=39.8406374501992, Blast_Score=182, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17553978, Length=195, Percent_Identity=30.7692307692308, Blast_Score=84, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6324037, Length=242, Percent_Identity=29.3388429752066, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323204, Length=198, Percent_Identity=33.8383838383838, Blast_Score=93, Evalue=6e-20,
Organism=Drosophila melanogaster, GI281364189, Length=195, Percent_Identity=30.7692307692308, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI281364187, Length=195, Percent_Identity=30.7692307692308, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI281364185, Length=195, Percent_Identity=30.7692307692308, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI281364183, Length=195, Percent_Identity=30.7692307692308, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI62471759, Length=195, Percent_Identity=30.7692307692308, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI17975520, Length=195, Percent_Identity=30.7692307692308, Blast_Score=88, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_GEOSL (P60344)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_952642.1
- ProteinModelPortal:   P60344
- SMR:   P60344
- GeneID:   2687358
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU1591
- NMPDR:   fig|243231.1.peg.1580
- TIGR:   GSU1591
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- ProtClustDB:   CLSK2522239
- BioCyc:   GSUL243231:GSU_1591-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 33017; Mature: 33017

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: ACT_SITE 38-38

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGFIVIDKPIGLTSHDVVARVRQTLRQKKAGHTGTLDPFATGVLPVAVGEGTKAIPYLD
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCEEEEECCCCCCCCCCC
ESTKVYRATLILGAATDTQDHTGQVVHAGDWRHLEPQSVRDVVSSFTGKLSQLPPMFSAL
CCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
KRDGVPLYKLARTGREVERERREIEVFSLVVDSIDLPLVTFTLSCSRGTYVRTLAHDMGE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHH
RLGCGAHLTELRRLSSGPFNLDRAISLERLKELAEAGNLADVLVSPSDALGHLHALPLTA
HCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHEECCCCC
QGAERVRRGMPPCREDVEHGAGTGIPAGTRLRLLRDGIVVAVADSLAGLWSSDTKNLRLL
CHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHCCEEEEEHHHHHHHCCCCCCCEEEE
RVFN
EECC
>Mature Secondary Structure
MDGFIVIDKPIGLTSHDVVARVRQTLRQKKAGHTGTLDPFATGVLPVAVGEGTKAIPYLD
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCEEEEECCCCCCCCCCC
ESTKVYRATLILGAATDTQDHTGQVVHAGDWRHLEPQSVRDVVSSFTGKLSQLPPMFSAL
CCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
KRDGVPLYKLARTGREVERERREIEVFSLVVDSIDLPLVTFTLSCSRGTYVRTLAHDMGE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHH
RLGCGAHLTELRRLSSGPFNLDRAISLERLKELAEAGNLADVLVSPSDALGHLHALPLTA
HCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHEECCCCC
QGAERVRRGMPPCREDVEHGAGTGIPAGTRLRLLRDGIVVAVADSLAGLWSSDTKNLRLL
CHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHCCEEEEEHHHHHHHCCCCCCCEEEE
RVFN
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA