Click here to switch to the map view.

The map label for this gene is bioB

Identifier: 39996684

GI number: 39996684

Start: 1736968

End: 1737957

Strand: Reverse

Name: bioB

Synonym: GSU1584

Alternate gene names: 39996684

Gene position: 1737957-1736968 (Counterclockwise)

Preceding gene: 39996713

Following gene: 39996683

Centisome position: 45.57

GC content: 60.51

Gene sequence:

>990_bases
ATGATCAACTACCCTGAATCACTAGCAAATCGCATCATCAAAGGAAGCACTCTCGACAAAGATGAAGCCAACGAGCTACT
CCTGCTGGAAGGCACCGATGCCAACGCCCTCTTCCTGGCGGCCAGTCGCGTCCGCGATCATTTCCTCGGTACCGGCGTGG
ACCTGTGCTCCATCATTAACGCCAAATCGGGCCGCTGCCCGGAAAACTGCGCGTTTTGCGCCCAATCGGCTCACCACGCC
ACCAACGCGCCCGTGTATCCTCTCGTCGACGAAGAACAGATCACCGCCTGCGCCAGAGAAGCAGCGGGGGCCGGATCCCA
TTGCTTCGGCATCGTAACCAGCGGCTCTGCCATCTCGCGCGGCGAAGAGCTGGACCGAATCTGCCGGGCCCTGCGGCGCA
TCCGCCGGGAGACGGCCATAGAGCCGTCCTGCTCCCTCGGTGTCATCGACTACGAAACCGCCCTGGCGCTTCGCGAGGCC
GGAGCCGTAACCTATCATCACAATCTTGAAACAGCCCGCAGCTTCTTCCCGAACGTCTGCACCACCCATGATTACGAAGA
GGATGTGGAAACAGTACGAGTCGCGAAGCGTGCAGGACTGAAGGTATGCTGCGGCGGCATTTTCGGTCTGGGAGAAACCC
CGGAACAACGGGTCGAAATGGCCCTCACCCTGCGTGAACTCGACGTGGATTCGATCCCTCTCAACTTCCTGAATCCCATC
GAAGGGACGCCTCTGGCCGGTGCGGACCGAATTACCCCGCTCGAATGCCTCAAGACCATCGCCGTGTACCGTCTCATCCT
TCCGGACCGGAAAATCGCCGTTTGCGGAGGCCGTGAGCGCAACCTGAGGGACCTGCAGTCGTGGATGTTTTTTGCCGGGG
CCAGCGGCACCATGATCGGCAACTATCTTACCACCACCGGTCGTCCGCCCGAGCAGGACTGGCAGATGCTGGCCGACCTG
GGGCTTACTGTGAGGCAATGCAATGGCTGA

Upstream 100 bases:

>100_bases
CAACCCATATTCTGTTGGCCATTGTCAACCTATCACTACACACAAGTTGACAATGGCCAACCGCCTGTGGTAATCCAAGG
ATGCGGAGGAACCACACACA

Downstream 100 bases:

>100_bases
GAAAGGAATTTTCATTACCGGAACTGATACCGGAGTGGGCAAAACAATCGTAGCGGCGACCATCGCCCGCCTGCTGCGAG
ACCGGGGCGTAAATGTGGGC

Product: biotin synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MINYPESLANRIIKGSTLDKDEANELLLLEGTDANALFLAASRVRDHFLGTGVDLCSIINAKSGRCPENCAFCAQSAHHA
TNAPVYPLVDEEQITACAREAAGAGSHCFGIVTSGSAISRGEELDRICRALRRIRRETAIEPSCSLGVIDYETALALREA
GAVTYHHNLETARSFFPNVCTTHDYEEDVETVRVAKRAGLKVCCGGIFGLGETPEQRVEMALTLRELDVDSIPLNFLNPI
EGTPLAGADRITPLECLKTIAVYRLILPDRKIAVCGGRERNLRDLQSWMFFAGASGTMIGNYLTTTGRPPEQDWQMLADL
GLTVRQCNG

Sequences:

>Translated_329_residues
MINYPESLANRIIKGSTLDKDEANELLLLEGTDANALFLAASRVRDHFLGTGVDLCSIINAKSGRCPENCAFCAQSAHHA
TNAPVYPLVDEEQITACAREAAGAGSHCFGIVTSGSAISRGEELDRICRALRRIRRETAIEPSCSLGVIDYETALALREA
GAVTYHHNLETARSFFPNVCTTHDYEEDVETVRVAKRAGLKVCCGGIFGLGETPEQRVEMALTLRELDVDSIPLNFLNPI
EGTPLAGADRITPLECLKTIAVYRLILPDRKIAVCGGRERNLRDLQSWMFFAGASGTMIGNYLTTTGRPPEQDWQMLADL
GLTVRQCNG
>Mature_329_residues
MINYPESLANRIIKGSTLDKDEANELLLLEGTDANALFLAASRVRDHFLGTGVDLCSIINAKSGRCPENCAFCAQSAHHA
TNAPVYPLVDEEQITACAREAAGAGSHCFGIVTSGSAISRGEELDRICRALRRIRRETAIEPSCSLGVIDYETALALREA
GAVTYHHNLETARSFFPNVCTTHDYEEDVETVRVAKRAGLKVCCGGIFGLGETPEQRVEMALTLRELDVDSIPLNFLNPI
EGTPLAGADRITPLECLKTIAVYRLILPDRKIAVCGGRERNLRDLQSWMFFAGASGTMIGNYLTTTGRPPEQDWQMLADL
GLTVRQCNG

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=304, Percent_Identity=33.8815789473684, Blast_Score=174, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6321725, Length=282, Percent_Identity=31.5602836879433, Blast_Score=147, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_GEOSL (Q74CT7)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_952635.1
- HSSP:   P12996
- ProteinModelPortal:   Q74CT7
- SMR:   Q74CT7
- GeneID:   2687302
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU1584
- NMPDR:   fig|243231.1.peg.1573
- TIGR:   GSU1584
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- ProtClustDB:   CLSK828421
- BioCyc:   GSUL243231:GSU_1584-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 35846; Mature: 35846

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINYPESLANRIIKGSTLDKDEANELLLLEGTDANALFLAASRVRDHFLGTGVDLCSIIN
CCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEHHHHHHHHHHHCCCHHHHHHHC
AKSGRCPENCAFCAQSAHHATNAPVYPLVDEEQITACAREAAGAGSHCFGIVTSGSAISR
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCHHHC
GEELDRICRALRRIRRETAIEPSCSLGVIDYETALALREAGAVTYHHNLETARSFFPNVC
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHCCCEEEECCHHHHHHHCCCCC
TTHDYEEDVETVRVAKRAGLKVCCGGIFGLGETPEQRVEMALTLRELDVDSIPLNFLNPI
CCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHCCCC
EGTPLAGADRITPLECLKTIAVYRLILPDRKIAVCGGRERNLRDLQSWMFFAGASGTMIG
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCEEC
NYLTTTGRPPEQDWQMLADLGLTVRQCNG
CEEECCCCCCHHHHHHHHHHCCEEEECCC
>Mature Secondary Structure
MINYPESLANRIIKGSTLDKDEANELLLLEGTDANALFLAASRVRDHFLGTGVDLCSIIN
CCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEHHHHHHHHHHHCCCHHHHHHHC
AKSGRCPENCAFCAQSAHHATNAPVYPLVDEEQITACAREAAGAGSHCFGIVTSGSAISR
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCHHHC
GEELDRICRALRRIRRETAIEPSCSLGVIDYETALALREAGAVTYHHNLETARSFFPNVC
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHCCCEEEECCHHHHHHHCCCCC
TTHDYEEDVETVRVAKRAGLKVCCGGIFGLGETPEQRVEMALTLRELDVDSIPLNFLNPI
CCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHCCCC
EGTPLAGADRITPLECLKTIAVYRLILPDRKIAVCGGRERNLRDLQSWMFFAGASGTMIG
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCEEC
NYLTTTGRPPEQDWQMLADLGLTVRQCNG
CEEECCCCCCHHHHHHHHHHCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA