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The map label for this gene is sigA [H]

Identifier: 39996625

GI number: 39996625

Start: 1671264

End: 1672250

Strand: Direct

Name: sigA [H]

Synonym: GSU1525

Alternate gene names: 39996625

Gene position: 1671264-1672250 (Clockwise)

Preceding gene: 39996624

Following gene: 39996626

Centisome position: 43.82

GC content: 57.35

Gene sequence:

>987_bases
ATGAGCGACATGAACGAACTGGATGAGAAAGAGCTGGATGCTCCGGATGAGCCCGGCGAGGACTTCATTGAGCCCGAGCC
GACGACCTTTGAGCTGGAGGCGGCCGAGGAAGAGGAAGAGCCCGAGGAGCCCGCTGAGGTCGGCAAGAACATAGAGCAGG
AGCACTTCGACGATGCAATCAAGCTCTATTTGCGCGAGATTCAGCGGACGCGGCTGCTGACCGCCGATGAGGAAAAAGAG
CTGGCCGGCCGTATCGCCAAGGGTGACAAGGCTGCCCGGGATAAGATGATCGAGTCGAATCTGCGGCTCGTGGTCAAGAT
TGCCAAGCGGTACATCAATCGGGGGCTCCCTTTTCTCGATCTGATCGAGGAGGGCAACATGGGGCTCATCAAGGCGGTGG
AGCGCTTCAAGCTCTCGAAGGAGTGCCGTTTCTCCACCTACGCCACCTGGTGGATCCGCCAATCCATCGAGCGCGCCCTC
GTGAATCAGTCGCGGACGATCAGACTGCCGGTCCATGTCTCCGACGACATTAACAAGATGCTTAAGATCACGCGTGAACT
GGTGCAGAGACTCAACCGCGAGCCGAGCGTGAAAGAAGTCGCCACCGAGATGAAGGTGAATTCCGCCTACGTCCGGCGGC
TGATGGTCCTCCTCAAAAAGACCTACTCCATCGAGCGCCCCATGGGGGAGAACAGCGATTATTTTCTTATCGACACCATT
GAGGATACGTCCACCGTCTCGCCGGCCGTGCTGCTTGAGGATCTCAACAAATACGAAATCGTGTCCAAGTGGTTTGCGTC
CCTGTCTGAGAACGAGCAGACGATCCTCACGCTCCGCTTTGGCCTGGAGGACAAGGAGCCCCAGACCCTCGACACCATCG
GTCGCAGCTTCGGCGTCACCCGTGAGCGTATCCGGCAGATCGAGGCCAAGTCTCTGGAAAAATTGAGAAAGTATATTGAG
GAAAGCGATGGCAACGCCATCGATTGA

Upstream 100 bases:

>100_bases
GGGAGAACATCACGGGATGCAGGTTCGTCAAACTGGTGGGCAAATTCGGTTGGAGCAGCGATGACTAAGCGCCGCCGGGC
GCTTTGCGACGGAGATCTCC

Downstream 100 bases:

>100_bases
GCCGGCTTGATGCCATCACGGAGGGGGAAGAAGCATGGATGAGCTGAAAAACATAATCCGCGACGTACCCGATTTCCCCA
AAAAGGGGATCATCTTCAAG

Product: RNA polymerase sigma-70 factor family

Products: NA

Alternate protein names: Sigma-A [H]

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MSDMNELDEKELDAPDEPGEDFIEPEPTTFELEAAEEEEEPEEPAEVGKNIEQEHFDDAIKLYLREIQRTRLLTADEEKE
LAGRIAKGDKAARDKMIESNLRLVVKIAKRYINRGLPFLDLIEEGNMGLIKAVERFKLSKECRFSTYATWWIRQSIERAL
VNQSRTIRLPVHVSDDINKMLKITRELVQRLNREPSVKEVATEMKVNSAYVRRLMVLLKKTYSIERPMGENSDYFLIDTI
EDTSTVSPAVLLEDLNKYEIVSKWFASLSENEQTILTLRFGLEDKEPQTLDTIGRSFGVTRERIRQIEAKSLEKLRKYIE
ESDGNAID

Sequences:

>Translated_328_residues
MSDMNELDEKELDAPDEPGEDFIEPEPTTFELEAAEEEEEPEEPAEVGKNIEQEHFDDAIKLYLREIQRTRLLTADEEKE
LAGRIAKGDKAARDKMIESNLRLVVKIAKRYINRGLPFLDLIEEGNMGLIKAVERFKLSKECRFSTYATWWIRQSIERAL
VNQSRTIRLPVHVSDDINKMLKITRELVQRLNREPSVKEVATEMKVNSAYVRRLMVLLKKTYSIERPMGENSDYFLIDTI
EDTSTVSPAVLLEDLNKYEIVSKWFASLSENEQTILTLRFGLEDKEPQTLDTIGRSFGVTRERIRQIEAKSLEKLRKYIE
ESDGNAID
>Mature_327_residues
SDMNELDEKELDAPDEPGEDFIEPEPTTFELEAAEEEEEPEEPAEVGKNIEQEHFDDAIKLYLREIQRTRLLTADEEKEL
AGRIAKGDKAARDKMIESNLRLVVKIAKRYINRGLPFLDLIEEGNMGLIKAVERFKLSKECRFSTYATWWIRQSIERALV
NQSRTIRLPVHVSDDINKMLKITRELVQRLNREPSVKEVATEMKVNSAYVRRLMVLLKKTYSIERPMGENSDYFLIDTIE
DTSTVSPAVLLEDLNKYEIVSKWFASLSENEQTILTLRFGLEDKEPQTLDTIGRSFGVTRERIRQIEAKSLEKLRKYIEE
SDGNAID

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=323, Percent_Identity=43.343653250774, Blast_Score=248, Evalue=3e-67,
Organism=Escherichia coli, GI1789448, Length=258, Percent_Identity=44.1860465116279, Blast_Score=218, Evalue=3e-58,
Organism=Escherichia coli, GI1789871, Length=276, Percent_Identity=31.5217391304348, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001909
- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 38020; Mature: 37888

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDMNELDEKELDAPDEPGEDFIEPEPTTFELEAAEEEEEPEEPAEVGKNIEQEHFDDAI
CCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHCCCCCHHHHHHHH
KLYLREIQRTRLLTADEEKELAGRIAKGDKAARDKMIESNLRLVVKIAKRYINRGLPFLD
HHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LIEEGNMGLIKAVERFKLSKECRFSTYATWWIRQSIERALVNQSRTIRLPVHVSDDINKM
HHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHH
LKITRELVQRLNREPSVKEVATEMKVNSAYVRRLMVLLKKTYSIERPMGENSDYFLIDTI
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEC
EDTSTVSPAVLLEDLNKYEIVSKWFASLSENEQTILTLRFGLEDKEPQTLDTIGRSFGVT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHCHH
RERIRQIEAKSLEKLRKYIEESDGNAID
HHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SDMNELDEKELDAPDEPGEDFIEPEPTTFELEAAEEEEEPEEPAEVGKNIEQEHFDDAI
CCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHCCCCCHHHHHHHH
KLYLREIQRTRLLTADEEKELAGRIAKGDKAARDKMIESNLRLVVKIAKRYINRGLPFLD
HHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LIEEGNMGLIKAVERFKLSKECRFSTYATWWIRQSIERALVNQSRTIRLPVHVSDDINKM
HHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHH
LKITRELVQRLNREPSVKEVATEMKVNSAYVRRLMVLLKKTYSIERPMGENSDYFLIDTI
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEC
EDTSTVSPAVLLEDLNKYEIVSKWFASLSENEQTILTLRFGLEDKEPQTLDTIGRSFGVT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHCHH
RERIRQIEAKSLEKLRKYIEESDGNAID
HHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA