Click here to switch to the map view.

The map label for this gene is pheT

Identifier: 39996620

GI number: 39996620

Start: 1666433

End: 1668838

Strand: Direct

Name: pheT

Synonym: GSU1520

Alternate gene names: 39996620

Gene position: 1666433-1668838 (Clockwise)

Preceding gene: 39996619

Following gene: 39996621

Centisome position: 43.69

GC content: 60.39

Gene sequence:

>2406_bases
ATGATCGTTACGTACAACTGGCTCAAGGAATTTGTCGAATGTGATCTCTCTACTCAGGAGCTTGGCGACCTGCTTACCAT
GCTCGGCCTCGAAGTGGAGGGGGTACGCGAGGTCGGTGGCGGCCTCGACCAGGTAGTGGTCGCCGTGGTGGAGGAGCGCC
GGAAGCATCCCAATGCGGACAAGCTTTCGCTCTGCAAGGTCAACAACGGCCGGGAGATCCTGGATATCGTCTGTGGAGCC
CAGAACTTCACTGCCGGCGACAAGGTCGCCCTGGCCCAGATCGGGGCCGTGCTGCCTGGTGACTTCAAGATCAAGCGCTC
CAAGATACGCGGCGAGGAGTCGTGCGGCATGCTCTGCTCCGAGCGCGAACTGGGGCTTTCCGCTGAATCGGAAGGGATCA
TGATCCTTCCGTCCGACCTGCCGCTCGGAGTTCCCCTGTTCGACGCCCTTGGCCTCAAGGACACGATCTTCGAGATCGGG
CTCACTCCCAACCGTGCCGATTGTCTCAGTGTTATCGGGGTGGCACGGGAGATTGCGGCAAAGCTCGGGAAGCGGATTAC
CTATCCGGGACACGCGGTTGTCGAGTCCGGTGAGCCGGTGACGCAGAAGGCAACGGTCATTGTGGAGGATCCCGAACTCT
GCCCGCGCTATACTGCCCGCTTTATCAGCGGATGCTCCATCGGTCCCTCGCCGGCATGGCTGGTTCGGCGCCTTGAGGCG
GTCGGAATGCGCTCCATCAACAACGTGGTCGACGTGACCAACTACGTCCTGATGGAGTATGGGCATCCTTTGCACGCCTT
TGATGCCGACCTGCTTGAGAACAGCACGATCGTTGTCCGTCGGGCAACGGACGGTGAGGTGTTTACCACCCTTGATGGTC
AGCAGCGCACCCTCACCGCCGGCGACCTTACTATCCGTGACGGAGCGAGGAGTGTTGCCCTCGCTGGTATCATGGGGGGA
GAGAATTCGGAAATCCGTGATACTACGACGAATATCCTGCTGGAGAGCGCTTACTTCAATCCCTCCGCCATCCGTCGTAC
CGCCAAGCGCCTGGGGCTCCACACCGAGTCGTCCCATCGTTTTGAGCGGGGGGCCGACGTGGCCATTGTGACCAGGGCCT
TGGACCGGGCTGCCGCGCTTCTGGCCGAACTGGCCGGCGGGACTGTTGCCGCGGGGATTATCGACGTTTATCCCACGCCT
GTTTCCCATCGGACGATCCGGTTCAGGGTGGACCGCTGTAATGCCCTTCTCGGGGTCGAGCTCTCCGCCAACGAAATGAA
GGCACTGTTTCATCACCTGGAATTTACCACAGTCACGGTTGAGCCCGGCATAATCGATGTGACGGTCCCGACATTCAGGG
TGGACCTGGAGCGCGAGATCGACCTGGTGGAGGAGGTTGCGCGGCTAAACGGCTATGACCGGATTGAAACGACCATGCCG
CGAGCCCGTGTCTTTTCCGATCGCCCCACAAAGCACCAGCGCATGGAGAGGCGGTGTCGCGATCTGATGGTGGGGCAGGG
GTTCAATGAAGTCATAACCTTCAGCTTCATGGCGCCGGGCGCCCTTGACCGCATGATGCTCGGCCCTGAGGATGGCCGGC
GTAGCGTGGTGGCGCTCAGGAATCCACTGGTGGACGAGCAGGCGGTCATGCGGACGACGCTGCTTCCCGGGCTCTTGGAG
GCCGCTTCCCGCAACCTGAATTACCGCAGTCTCGACCTGCGTCTCTTTGAGCTGCGGCGCGTGTATCTGCGGGTGGAAGG
GGAGCAGCTCCCCAATGAGCCACTGGTCCTTGCCGGGCTCATGACCGGCAGACGCTACCCTGAAGGGTGGAACCAGGAAA
AGCATCCTCTTGATTTCTATGATGTGAAGGGCGTTGTGGAGGCGGTTCTCGACGCATTTTCGGTCAGCGGCGCGAGCTAT
TCGTCTGACGACACCGATGTCTTTTACCATCCCGGCAAATCGTGCACGGTCAAGTGCGGCGATTTGATCCTGGGCTCTCT
CGGCGAACTCCATCCCGATGTGCAGGACAACTTCGGGATCGATCAGCCGGTATTCTACTTCGAGCTTAATTTCGAGCGGC
TCCTGTCCGCAGCGCGCGCCGCCTCGGCCGTTGTCCCGCCGTCACGCTTCCCGGACACGTTCAGGGATATTGCCATACTT
GTGGCCGATGAGACGCCGGCTGCGGACATTGTCCGGTGCATCGACGGGCTGCGTATCCGGGAAATCGAAAGTGCTGCAGT
GTTTGACCTCTACAAAGGTGTCCACGTTCCCGAAGGGAAGAAGAGCATCGCGGTGCGGGTTCGCTATCGTTCCACAGAGA
AAACCCTGAGCGATGACGAGGTTTCACCGCTGCACCAGAAGGTCGTTGACAGCCTTGTCGCGAAGCTCGGAGCGACGATT
CGTTAA

Upstream 100 bases:

>100_bases
AATGATGTGCGGTTCCTGCAGCAATTTAGATAATCTGAATAGTTGGGCTCGTATTCTTCTGGTGAAACTTTAACTACTGC
ATCTCGGATAGAGTGAGAAT

Downstream 100 bases:

>100_bases
AAACTGGTTGATTCTGCCGAATCGCTGTGATATAAAACCAGAGCTTCAGTTGGCCGGATAGTGGCTAGATTATGCAATAA
TTTTGAGGGAGTATATGACG

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS

Number of amino acids: Translated: 801; Mature: 801

Protein sequence:

>801_residues
MIVTYNWLKEFVECDLSTQELGDLLTMLGLEVEGVREVGGGLDQVVVAVVEERRKHPNADKLSLCKVNNGREILDIVCGA
QNFTAGDKVALAQIGAVLPGDFKIKRSKIRGEESCGMLCSERELGLSAESEGIMILPSDLPLGVPLFDALGLKDTIFEIG
LTPNRADCLSVIGVAREIAAKLGKRITYPGHAVVESGEPVTQKATVIVEDPELCPRYTARFISGCSIGPSPAWLVRRLEA
VGMRSINNVVDVTNYVLMEYGHPLHAFDADLLENSTIVVRRATDGEVFTTLDGQQRTLTAGDLTIRDGARSVALAGIMGG
ENSEIRDTTTNILLESAYFNPSAIRRTAKRLGLHTESSHRFERGADVAIVTRALDRAAALLAELAGGTVAAGIIDVYPTP
VSHRTIRFRVDRCNALLGVELSANEMKALFHHLEFTTVTVEPGIIDVTVPTFRVDLEREIDLVEEVARLNGYDRIETTMP
RARVFSDRPTKHQRMERRCRDLMVGQGFNEVITFSFMAPGALDRMMLGPEDGRRSVVALRNPLVDEQAVMRTTLLPGLLE
AASRNLNYRSLDLRLFELRRVYLRVEGEQLPNEPLVLAGLMTGRRYPEGWNQEKHPLDFYDVKGVVEAVLDAFSVSGASY
SSDDTDVFYHPGKSCTVKCGDLILGSLGELHPDVQDNFGIDQPVFYFELNFERLLSAARAASAVVPPSRFPDTFRDIAIL
VADETPAADIVRCIDGLRIREIESAAVFDLYKGVHVPEGKKSIAVRVRYRSTEKTLSDDEVSPLHQKVVDSLVAKLGATI
R

Sequences:

>Translated_801_residues
MIVTYNWLKEFVECDLSTQELGDLLTMLGLEVEGVREVGGGLDQVVVAVVEERRKHPNADKLSLCKVNNGREILDIVCGA
QNFTAGDKVALAQIGAVLPGDFKIKRSKIRGEESCGMLCSERELGLSAESEGIMILPSDLPLGVPLFDALGLKDTIFEIG
LTPNRADCLSVIGVAREIAAKLGKRITYPGHAVVESGEPVTQKATVIVEDPELCPRYTARFISGCSIGPSPAWLVRRLEA
VGMRSINNVVDVTNYVLMEYGHPLHAFDADLLENSTIVVRRATDGEVFTTLDGQQRTLTAGDLTIRDGARSVALAGIMGG
ENSEIRDTTTNILLESAYFNPSAIRRTAKRLGLHTESSHRFERGADVAIVTRALDRAAALLAELAGGTVAAGIIDVYPTP
VSHRTIRFRVDRCNALLGVELSANEMKALFHHLEFTTVTVEPGIIDVTVPTFRVDLEREIDLVEEVARLNGYDRIETTMP
RARVFSDRPTKHQRMERRCRDLMVGQGFNEVITFSFMAPGALDRMMLGPEDGRRSVVALRNPLVDEQAVMRTTLLPGLLE
AASRNLNYRSLDLRLFELRRVYLRVEGEQLPNEPLVLAGLMTGRRYPEGWNQEKHPLDFYDVKGVVEAVLDAFSVSGASY
SSDDTDVFYHPGKSCTVKCGDLILGSLGELHPDVQDNFGIDQPVFYFELNFERLLSAARAASAVVPPSRFPDTFRDIAIL
VADETPAADIVRCIDGLRIREIESAAVFDLYKGVHVPEGKKSIAVRVRYRSTEKTLSDDEVSPLHQKVVDSLVAKLGATI
R
>Mature_801_residues
MIVTYNWLKEFVECDLSTQELGDLLTMLGLEVEGVREVGGGLDQVVVAVVEERRKHPNADKLSLCKVNNGREILDIVCGA
QNFTAGDKVALAQIGAVLPGDFKIKRSKIRGEESCGMLCSERELGLSAESEGIMILPSDLPLGVPLFDALGLKDTIFEIG
LTPNRADCLSVIGVAREIAAKLGKRITYPGHAVVESGEPVTQKATVIVEDPELCPRYTARFISGCSIGPSPAWLVRRLEA
VGMRSINNVVDVTNYVLMEYGHPLHAFDADLLENSTIVVRRATDGEVFTTLDGQQRTLTAGDLTIRDGARSVALAGIMGG
ENSEIRDTTTNILLESAYFNPSAIRRTAKRLGLHTESSHRFERGADVAIVTRALDRAAALLAELAGGTVAAGIIDVYPTP
VSHRTIRFRVDRCNALLGVELSANEMKALFHHLEFTTVTVEPGIIDVTVPTFRVDLEREIDLVEEVARLNGYDRIETTMP
RARVFSDRPTKHQRMERRCRDLMVGQGFNEVITFSFMAPGALDRMMLGPEDGRRSVVALRNPLVDEQAVMRTTLLPGLLE
AASRNLNYRSLDLRLFELRRVYLRVEGEQLPNEPLVLAGLMTGRRYPEGWNQEKHPLDFYDVKGVVEAVLDAFSVSGASY
SSDDTDVFYHPGKSCTVKCGDLILGSLGELHPDVQDNFGIDQPVFYFELNFERLLSAARAASAVVPPSRFPDTFRDIAIL
VADETPAADIVRCIDGLRIREIESAAVFDLYKGVHVPEGKKSIAVRVRYRSTEKTLSDDEVSPLHQKVVDSLVAKLGATI
R

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI124028525, Length=398, Percent_Identity=23.6180904522613, Blast_Score=83, Evalue=9e-16,
Organism=Escherichia coli, GI1788006, Length=802, Percent_Identity=39.1521197007481, Blast_Score=515, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI17533403, Length=314, Percent_Identity=25.4777070063694, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21355211, Length=398, Percent_Identity=25.8793969849246, Blast_Score=86, Evalue=8e-17,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFB_GEOSL (Q74CZ9)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_952571.1
- ProteinModelPortal:   Q74CZ9
- SMR:   Q74CZ9
- GeneID:   2687692
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU1520
- NMPDR:   fig|243231.1.peg.1509
- TIGR:   GSU1520
- HOGENOM:   HBG399638
- OMA:   ADKLRVC
- ProtClustDB:   PRK00629
- BioCyc:   GSUL243231:GSU_1520-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00283
- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547
- Gene3D:   G3DSA:3.50.40.10
- Gene3D:   G3DSA:3.30.56.20
- Gene3D:   G3DSA:3.30.70.380
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00873
- SMART:   SM00874
- SMART:   SM00896
- TIGRFAMs:   TIGR00472

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind; SSF56037 B3_4; SSF54991 Fdx_AntiC_bd; SSF50249 Nucleic_acid_OB; SSF46955 Putativ_DNA_bind

EC number: =6.1.1.20

Molecular weight: Translated: 88127; Mature: 88127

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS51483 B5; PS51447 FDX_ACB; PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVTYNWLKEFVECDLSTQELGDLLTMLGLEVEGVREVGGGLDQVVVAVVEERRKHPNAD
CEEEHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCC
KLSLCKVNNGREILDIVCGAQNFTAGDKVALAQIGAVLPGDFKIKRSKIRGEESCGMLCS
CEEEEEECCCCEEHHHHCCCCCCCCCCHHHHHHHCCCCCCCCEEEHHHCCCCHHHCCEEC
ERELGLSAESEGIMILPSDLPLGVPLFDALGLKDTIFEIGLTPNRADCLSVIGVAREIAA
CCCCCCCCCCCCEEEEECCCCCCCCHHHHCCCHHHHHEECCCCCHHHHHHHHHHHHHHHH
KLGKRITYPGHAVVESGEPVTQKATVIVEDPELCPRYTARFISGCSIGPSPAWLVRRLEA
HHCCEECCCCCEEEECCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHH
VGMRSINNVVDVTNYVLMEYGHPLHAFDADLLENSTIVVRRATDGEVFTTLDGQQRTLTA
HHHHHHHHHHHHHHHHHHHCCCCCHHHCHHHHCCCEEEEEECCCCCEEEEECCCCEEEEE
GDLTIRDGARSVALAGIMGGENSEIRDTTTNILLESAYFNPSAIRRTAKRLGLHTESSHR
CCEEEECCCCEEEEEEEECCCCCCCHHHHHHHEEEECCCCHHHHHHHHHHHCCCCCCCCC
FERGADVAIVTRALDRAAALLAELAGGTVAAGIIDVYPTPVSHRTIRFRVDRCNALLGVE
HHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEEEEECCCEEEEEE
LSANEMKALFHHLEFTTVTVEPGIIDVTVPTFRVDLEREIDLVEEVARLNGYDRIETTMP
ECHHHHHHHHHHHEEEEEEECCCEEEEECCEEEECHHHHHHHHHHHHHCCCCCHHHHCCC
RARVFSDRPTKHQRMERRCRDLMVGQGFNEVITFSFMAPGALDRMMLGPEDGRRSVVALR
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHEEEEEECCCHHHHHEECCCCCCCCCHHHH
NPLVDEQAVMRTTLLPGLLEAASRNLNYRSLDLRLFELRRVYLRVEGEQLPNEPLVLAGL
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHEEHEEECCCCCCCCCEEEEEE
MTGRRYPEGWNQEKHPLDFYDVKGVVEAVLDAFSVSGASYSSDDTDVFYHPGKSCTVKCG
HHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEEHH
DLILGSLGELHPDVQDNFGIDQPVFYFELNFERLLSAARAASAVVPPSRFPDTFRDIAIL
HHHHHHHHHCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHCCCCCCCCCCCHHCEEEE
VADETPAADIVRCIDGLRIREIESAAVFDLYKGVHVPEGKKSIAVRVRYRSTEKTLSDDE
EECCCCHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCC
VSPLHQKVVDSLVAKLGATIR
CCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIVTYNWLKEFVECDLSTQELGDLLTMLGLEVEGVREVGGGLDQVVVAVVEERRKHPNAD
CEEEHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCC
KLSLCKVNNGREILDIVCGAQNFTAGDKVALAQIGAVLPGDFKIKRSKIRGEESCGMLCS
CEEEEEECCCCEEHHHHCCCCCCCCCCHHHHHHHCCCCCCCCEEEHHHCCCCHHHCCEEC
ERELGLSAESEGIMILPSDLPLGVPLFDALGLKDTIFEIGLTPNRADCLSVIGVAREIAA
CCCCCCCCCCCCEEEEECCCCCCCCHHHHCCCHHHHHEECCCCCHHHHHHHHHHHHHHHH
KLGKRITYPGHAVVESGEPVTQKATVIVEDPELCPRYTARFISGCSIGPSPAWLVRRLEA
HHCCEECCCCCEEEECCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHH
VGMRSINNVVDVTNYVLMEYGHPLHAFDADLLENSTIVVRRATDGEVFTTLDGQQRTLTA
HHHHHHHHHHHHHHHHHHHCCCCCHHHCHHHHCCCEEEEEECCCCCEEEEECCCCEEEEE
GDLTIRDGARSVALAGIMGGENSEIRDTTTNILLESAYFNPSAIRRTAKRLGLHTESSHR
CCEEEECCCCEEEEEEEECCCCCCCHHHHHHHEEEECCCCHHHHHHHHHHHCCCCCCCCC
FERGADVAIVTRALDRAAALLAELAGGTVAAGIIDVYPTPVSHRTIRFRVDRCNALLGVE
HHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEEEEECCCEEEEEE
LSANEMKALFHHLEFTTVTVEPGIIDVTVPTFRVDLEREIDLVEEVARLNGYDRIETTMP
ECHHHHHHHHHHHEEEEEEECCCEEEEECCEEEECHHHHHHHHHHHHHCCCCCHHHHCCC
RARVFSDRPTKHQRMERRCRDLMVGQGFNEVITFSFMAPGALDRMMLGPEDGRRSVVALR
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHEEEEEECCCHHHHHEECCCCCCCCCHHHH
NPLVDEQAVMRTTLLPGLLEAASRNLNYRSLDLRLFELRRVYLRVEGEQLPNEPLVLAGL
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHEEHEEECCCCCCCCCEEEEEE
MTGRRYPEGWNQEKHPLDFYDVKGVVEAVLDAFSVSGASYSSDDTDVFYHPGKSCTVKCG
HHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEEHH
DLILGSLGELHPDVQDNFGIDQPVFYFELNFERLLSAARAASAVVPPSRFPDTFRDIAIL
HHHHHHHHHCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHCCCCCCCCCCCHHCEEEE
VADETPAADIVRCIDGLRIREIESAAVFDLYKGVHVPEGKKSIAVRVRYRSTEKTLSDDE
EECCCCHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCC
VSPLHQKVVDSLVAKLGATIR
CCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA