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The map label for this gene is pheS

Identifier: 39996619

GI number: 39996619

Start: 1665346

End: 1666365

Strand: Direct

Name: pheS

Synonym: GSU1519

Alternate gene names: 39996619

Gene position: 1665346-1666365 (Clockwise)

Preceding gene: 39996618

Following gene: 39996620

Centisome position: 43.66

GC content: 59.22

Gene sequence:

>1020_bases
ATGAAAGAAACGCTTGATCAACTGATGCAATCGGCCCTTGCGGAGATTGCCGGCGCAGCCTCCGAGGACGCCCTGCAGGA
GTTGCGCGTCAGGTACCTGGGGAAAAAGGGTGAACTGACGGCGGTCATGAAGGGGCTTGGTTCGCTCTCGCCCGAAGAGC
GTCCCCGCATGGGCCAAATGGTCAACACGGTCAAGGACCGGCTCGAGACCACGCTGGAGGAAACCCTGCAACGGGTCAGG
GCTGCGGCTAAAAAGGAGCGCCTTGAGCGGGAACGCCTCGACGTGACGCTCCCCGGTCGTACGTCGCCTTTGGGAACCAA
GCACCCCATCTCGCTCGTCATCGAGGAAATTTCGGACATCTTCGCCGGCCTCGGCTTTCAGGTGGCCGAAGGCCCCGAGG
TGGAGCTGGACTTTTACAACTTCGAGGCTCTCAATTTTCCCAAGGATCACCCGGCCCGGGACATGCAGGATACCTTTTTC
GTTGATGACGACATCCTGCTGCGCACCCACACCTCTCCGGTCCAGGTCCGGACCATGCTCAAGCACGCACCTCCGGTCAG
GATCATCTCGCCGGGCACGGTTTACCGGTGCGACTCCGATGCCACCCACTCTCCCATGTTCCATCAGATCGAAGGTTTCA
TGGTGGACAAGGGTGTTACCTTCGGCGATCTGAAGGGGATACTGACCAACTTTGTGAACCAGTTCTTCGGCGCCGGAACA
GGGGTTCGGTTACGTCCCTCATTCTTCCCCTTCACCGAGCCGAGCGCCGAGGTGGACATTGCCTGCGTCATGTGTAAAGG
GCAGGGGTGCCGGGTCTGCAAGCAGAGCGGCTGGCTCGAAATTCTGGGTGCCGGCATGATCGATCCGGAAGTCTATCGTC
ATGTTGGCTATGATCCCGAGTCGATCTCCGGCTTTGCGTTCGGCATGGGGATCGAGCGGGTCGCCATGCTCAAGTACGGC
ATCGGGGATCTGCGCCTGTTCTTCGATAATGATGTGCGGTTCCTGCAGCAATTTAGATAA

Upstream 100 bases:

>100_bases
CAACCCTCGCCAAAGCCCAGTTTTAGTGAAATCCTGAGGAATACAAAAAGAAATGGGATGCTCTGCGTTCCATTTCTTTT
TTTGTAAAGGGATACCGGAC

Downstream 100 bases:

>100_bases
TCTGAATAGTTGGGCTCGTATTCTTCTGGTGAAACTTTAACTACTGCATCTCGGATAGAGTGAGAATATGATCGTTACGT
ACAACTGGCTCAAGGAATTT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MKETLDQLMQSALAEIAGAASEDALQELRVRYLGKKGELTAVMKGLGSLSPEERPRMGQMVNTVKDRLETTLEETLQRVR
AAAKKERLERERLDVTLPGRTSPLGTKHPISLVIEEISDIFAGLGFQVAEGPEVELDFYNFEALNFPKDHPARDMQDTFF
VDDDILLRTHTSPVQVRTMLKHAPPVRIISPGTVYRCDSDATHSPMFHQIEGFMVDKGVTFGDLKGILTNFVNQFFGAGT
GVRLRPSFFPFTEPSAEVDIACVMCKGQGCRVCKQSGWLEILGAGMIDPEVYRHVGYDPESISGFAFGMGIERVAMLKYG
IGDLRLFFDNDVRFLQQFR

Sequences:

>Translated_339_residues
MKETLDQLMQSALAEIAGAASEDALQELRVRYLGKKGELTAVMKGLGSLSPEERPRMGQMVNTVKDRLETTLEETLQRVR
AAAKKERLERERLDVTLPGRTSPLGTKHPISLVIEEISDIFAGLGFQVAEGPEVELDFYNFEALNFPKDHPARDMQDTFF
VDDDILLRTHTSPVQVRTMLKHAPPVRIISPGTVYRCDSDATHSPMFHQIEGFMVDKGVTFGDLKGILTNFVNQFFGAGT
GVRLRPSFFPFTEPSAEVDIACVMCKGQGCRVCKQSGWLEILGAGMIDPEVYRHVGYDPESISGFAFGMGIERVAMLKYG
IGDLRLFFDNDVRFLQQFR
>Mature_339_residues
MKETLDQLMQSALAEIAGAASEDALQELRVRYLGKKGELTAVMKGLGSLSPEERPRMGQMVNTVKDRLETTLEETLQRVR
AAAKKERLERERLDVTLPGRTSPLGTKHPISLVIEEISDIFAGLGFQVAEGPEVELDFYNFEALNFPKDHPARDMQDTFF
VDDDILLRTHTSPVQVRTMLKHAPPVRIISPGTVYRCDSDATHSPMFHQIEGFMVDKGVTFGDLKGILTNFVNQFFGAGT
GVRLRPSFFPFTEPSAEVDIACVMCKGQGCRVCKQSGWLEILGAGMIDPEVYRHVGYDPESISGFAFGMGIERVAMLKYG
IGDLRLFFDNDVRFLQQFR

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=278, Percent_Identity=32.3741007194245, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI5729820, Length=289, Percent_Identity=30.4498269896194, Blast_Score=115, Evalue=5e-26,
Organism=Escherichia coli, GI1788007, Length=335, Percent_Identity=51.044776119403, Blast_Score=346, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI17508957, Length=273, Percent_Identity=32.2344322344322, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI32563657, Length=273, Percent_Identity=32.2344322344322, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI32566635, Length=256, Percent_Identity=32.8125, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6321087, Length=370, Percent_Identity=30, Blast_Score=138, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6325304, Length=97, Percent_Identity=38.1443298969072, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI18858079, Length=268, Percent_Identity=32.089552238806, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17137424, Length=299, Percent_Identity=28.7625418060201, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_GEOSL (Q74D00)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_952570.1
- ProteinModelPortal:   Q74D00
- SMR:   Q74D00
- GeneID:   2687703
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU1519
- NMPDR:   fig|243231.1.peg.1508
- TIGR:   GSU1519
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- BioCyc:   GSUL243231:GSU_1519-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 37889; Mature: 37889

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKETLDQLMQSALAEIAGAASEDALQELRVRYLGKKGELTAVMKGLGSLSPEERPRMGQM
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHHH
VNTVKDRLETTLEETLQRVRAAAKKERLERERLDVTLPGRTSPLGTKHPISLVIEEISDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCHHHHHHHHHHHH
FAGLGFQVAEGPEVELDFYNFEALNFPKDHPARDMQDTFFVDDDILLRTHTSPVQVRTML
HHCCCCEECCCCCEEEEEEECEEECCCCCCCCCCCCCCEEECCCEEEEECCCHHHHHHHH
KHAPPVRIISPGTVYRCDSDATHSPMFHQIEGFMVDKGVTFGDLKGILTNFVNQFFGAGT
HCCCCEEEECCCEEEECCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHCCCC
GVRLRPSFFPFTEPSAEVDIACVMCKGQGCRVCKQSGWLEILGAGMIDPEVYRHVGYDPE
CCEECCCCCCCCCCCCCEEEEEEEECCCCCHHHHHCCCCEEECCCCCCHHHHHHCCCCCC
SISGFAFGMGIERVAMLKYGIGDLRLFFDNDVRFLQQFR
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCC
>Mature Secondary Structure
MKETLDQLMQSALAEIAGAASEDALQELRVRYLGKKGELTAVMKGLGSLSPEERPRMGQM
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHHH
VNTVKDRLETTLEETLQRVRAAAKKERLERERLDVTLPGRTSPLGTKHPISLVIEEISDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCHHHHHHHHHHHH
FAGLGFQVAEGPEVELDFYNFEALNFPKDHPARDMQDTFFVDDDILLRTHTSPVQVRTML
HHCCCCEECCCCCEEEEEEECEEECCCCCCCCCCCCCCEEECCCEEEEECCCHHHHHHHH
KHAPPVRIISPGTVYRCDSDATHSPMFHQIEGFMVDKGVTFGDLKGILTNFVNQFFGAGT
HCCCCEEEECCCEEEECCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHCCCC
GVRLRPSFFPFTEPSAEVDIACVMCKGQGCRVCKQSGWLEILGAGMIDPEVYRHVGYDPE
CCEECCCCCCCCCCCCCEEEEEEEECCCCCHHHHHCCCCEEECCCCCCHHHHHHCCCCCC
SISGFAFGMGIERVAMLKYGIGDLRLFFDNDVRFLQQFR
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA