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The map label for this gene is trmJ [H]

Identifier: 39996575

GI number: 39996575

Start: 1616582

End: 1617349

Strand: Direct

Name: trmJ [H]

Synonym: GSU1475

Alternate gene names: 39996575

Gene position: 1616582-1617349 (Clockwise)

Preceding gene: 39996574

Following gene: 39996576

Centisome position: 42.38

GC content: 59.9

Gene sequence:

>768_bases
ATGTCGGATCATGAAGTGACCGGAGGCGTTTCCCTGAAGGAGCGGGTCGCCATAGTACTGGTGGAGCCGCAGAGCCCCGG
CAATGTGGGGATGGTGTGTCGCGCCATGAAGAACATGGGGCTTGGCGAACTGCGAATCGTAAAGGGGTGCCGTCTCGATC
ACCCGGAAGCACTCAAGTTTGCCGTTTCCGCCCGTGATCTTCTTGAGAGGGCCAGAGTATTCGAGTCCCTTGAAGATGCC
CTGTCTGATACGGAACTGTCCGTGGCCACGACCCGTCGCCACGGCAAATACCGCCAGGAGATCTTTTCACCGAAGGAGAT
CGTGTCGAAGTTTGCCGCCAATCTTGCAGGCAACCGCGCCGCCCTGGTCTTCGGCCGCGAAGATAACGGTTTGACCACCG
ATGAGCTTTCCCTGTGCCGCTGGCATGCCACGATCCCCTCGTCGGAGGAGTACGGTTCTTTGAATCTGGCCCAGGCAGTG
ATGGTGTTCTGTTACGAGCTGTTCACCTCCCTCGGCAGCCGGACAGTGGGCGGCGAAGATCGTCCCCTTGCGGGATCAGC
CGAAACGGAGCCCCTCTTCGAGCATATGGAGCGCACCCTGATGCGTATCGGGTATCTCAATCCCCAGAATCCCGCCCATC
TGATGCGTTCGCTGCGGCGCATCTTTGCACGCTCCGAGTTGGACAGCCGCGAGGTGGCCATCCTGCGCGGGATGATGACC
CAGATCGATTGGGCCGCCGACCAGTTCAAGGGAAAGAAGGGAGCATGA

Upstream 100 bases:

>100_bases
GTTTCACTTTATCCATGCCCGCATGAGAAGGAGACCCGCCCGTGAACGGCTCAGGCGAATGAGGCAACGGGAGTTCGATT
GATGGGAGGAATGAAGAACG

Downstream 100 bases:

>100_bases
CCATCACGACCCTGCTCGGTCTTCTGGCGGGCACCCTTACGAGTGTGGCCGCCATCCCCCAGGTGGTCAGGGCCTTCAGA
ACCAAACGGGTACGCGACAT

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MSDHEVTGGVSLKERVAIVLVEPQSPGNVGMVCRAMKNMGLGELRIVKGCRLDHPEALKFAVSARDLLERARVFESLEDA
LSDTELSVATTRRHGKYRQEIFSPKEIVSKFAANLAGNRAALVFGREDNGLTTDELSLCRWHATIPSSEEYGSLNLAQAV
MVFCYELFTSLGSRTVGGEDRPLAGSAETEPLFEHMERTLMRIGYLNPQNPAHLMRSLRRIFARSELDSREVAILRGMMT
QIDWAADQFKGKKGA

Sequences:

>Translated_255_residues
MSDHEVTGGVSLKERVAIVLVEPQSPGNVGMVCRAMKNMGLGELRIVKGCRLDHPEALKFAVSARDLLERARVFESLEDA
LSDTELSVATTRRHGKYRQEIFSPKEIVSKFAANLAGNRAALVFGREDNGLTTDELSLCRWHATIPSSEEYGSLNLAQAV
MVFCYELFTSLGSRTVGGEDRPLAGSAETEPLFEHMERTLMRIGYLNPQNPAHLMRSLRRIFARSELDSREVAILRGMMT
QIDWAADQFKGKKGA
>Mature_254_residues
SDHEVTGGVSLKERVAIVLVEPQSPGNVGMVCRAMKNMGLGELRIVKGCRLDHPEALKFAVSARDLLERARVFESLEDAL
SDTELSVATTRRHGKYRQEIFSPKEIVSKFAANLAGNRAALVFGREDNGLTTDELSLCRWHATIPSSEEYGSLNLAQAVM
VFCYELFTSLGSRTVGGEDRPLAGSAETEPLFEHMERTLMRIGYLNPQNPAHLMRSLRRIFARSELDSREVAILRGMMTQ
IDWAADQFKGKKGA

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=246, Percent_Identity=36.9918699186992, Blast_Score=153, Evalue=8e-39,
Organism=Escherichia coli, GI1790865, Length=156, Percent_Identity=40.3846153846154, Blast_Score=113, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28315; Mature: 28184

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDHEVTGGVSLKERVAIVLVEPQSPGNVGMVCRAMKNMGLGELRIVKGCRLDHPEALKF
CCCCCCCCCCCHHHCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHH
AVSARDLLERARVFESLEDALSDTELSVATTRRHGKYRQEIFSPKEIVSKFAANLAGNRA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCE
ALVFGREDNGLTTDELSLCRWHATIPSSEEYGSLNLAQAVMVFCYELFTSLGSRTVGGED
EEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
RPLAGSAETEPLFEHMERTLMRIGYLNPQNPAHLMRSLRRIFARSELDSREVAILRGMMT
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QIDWAADQFKGKKGA
HHHHHHHHCCCCCCC
>Mature Secondary Structure 
SDHEVTGGVSLKERVAIVLVEPQSPGNVGMVCRAMKNMGLGELRIVKGCRLDHPEALKF
CCCCCCCCCCHHHCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHH
AVSARDLLERARVFESLEDALSDTELSVATTRRHGKYRQEIFSPKEIVSKFAANLAGNRA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCE
ALVFGREDNGLTTDELSLCRWHATIPSSEEYGSLNLAQAVMVFCYELFTSLGSRTVGGED
EEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
RPLAGSAETEPLFEHMERTLMRIGYLNPQNPAHLMRSLRRIFARSELDSREVAILRGMMT
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QIDWAADQFKGKKGA
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA