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The map label for this gene is mdh [H]

Identifier: 39996566

GI number: 39996566

Start: 1608804

End: 1609757

Strand: Direct

Name: mdh [H]

Synonym: GSU1466

Alternate gene names: 39996566

Gene position: 1608804-1609757 (Clockwise)

Preceding gene: 39996565

Following gene: 39996567

Centisome position: 42.18

GC content: 58.07

Gene sequence:

>954_bases
ATGGCACGTAAGAAGATTGCTCTCATTGGTGGTGGCCAGATCGGTGGTGTGCTTGCTCAACTCTGCGCTCTCCGTGAACT
TGGCGACGTCGTCCTGTTCGATATCGTAGAAGGCCTTCCCCAGGGGAAATGCCTGGATATCGCCGAAGCTTCTCCTGTTG
ACGGCTTTGACGTCTGCCTGAAGGGTACCAACAGCTACGAGGATATCGCTGGCGCCGACGTTGTCATCGTGACTGCGGGC
CTTCCCCGTAAGCCCGGCATGAGCCGTGACGACCTCATCGAGGTCAACTCCAAGATCATGACGTCGGTTGCCGAGGGGAT
CAAGCAGTACGCACCCAATTCCTTCGTGATTGTAATTTCCAACCCTCTGGACGCCATGGTTACCCTCTGCCAGAAGGTCA
CCGGTTTCCCCTACAACCGCGTCATCGGTCAGGCCGGAGTTCTCGACTCCGCCCGTTTTGCCACCTTCATCGCCTGGGAA
CTTGGCGTGTCCGTAAAGGACGTCACCGCCATGACTCTTGGCGGCCACGGCGACGACATGGTGCCGCTGGTTCGCTACGC
CAGCGTCAAAGGTATCCCGGTCATGGAACTCCTTGAGCGCAAGTACGGCAGTAAGGAGAAGGCGAAGGAAGTCATGGACG
CCATGGTCAACCGTACCCGTCTGGCGGGCGGCGAAGTCGTTGCCCTCCTCAAGACCGGTTCCGCCTTCTACAGCCCTGCG
TCCAGCGCCATTGCCATGGCCGAGTCTATCCTCAAGGATCAGAAGCGGGTTCTTCCGACCTGTGCCTACCTCCAGGGAGA
GTTCGGTGTCAACGGCTACTATGTGGGCGTACCCTGCGTGCTCGGCGAGAAGGGTATCGAGAATATCATTGAGTTCTCTC
TCGACGCAGAAGAACAAGCGATGATGGACAAGTCGGTCGCAGCGGTAAAGAGCCTCGTCGACAGCCTCAAGTAA

Upstream 100 bases:

>100_bases
GTTGCCAAGGCAATGCGCCCGAGCGCCGCTCTCAACGCGATTATCGACGCTATCTAGTGACGTAGGAAGTACACATATCT
GCTAAAGGAGGAAGATCAGT

Downstream 100 bases:

>100_bases
GGTGTTGTTACCCATGACGCAGCAAGGCAAAGAAGGGCCCTGTGCCCTTCTTTTGCCTTATGGGGGGAGTTGACGTAGTC
CGGTCGGTTCCCAATTGAGA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MARKKIALIGGGQIGGVLAQLCALRELGDVVLFDIVEGLPQGKCLDIAEASPVDGFDVCLKGTNSYEDIAGADVVIVTAG
LPRKPGMSRDDLIEVNSKIMTSVAEGIKQYAPNSFVIVISNPLDAMVTLCQKVTGFPYNRVIGQAGVLDSARFATFIAWE
LGVSVKDVTAMTLGGHGDDMVPLVRYASVKGIPVMELLERKYGSKEKAKEVMDAMVNRTRLAGGEVVALLKTGSAFYSPA
SSAIAMAESILKDQKRVLPTCAYLQGEFGVNGYYVGVPCVLGEKGIENIIEFSLDAEEQAMMDKSVAAVKSLVDSLK

Sequences:

>Translated_317_residues
MARKKIALIGGGQIGGVLAQLCALRELGDVVLFDIVEGLPQGKCLDIAEASPVDGFDVCLKGTNSYEDIAGADVVIVTAG
LPRKPGMSRDDLIEVNSKIMTSVAEGIKQYAPNSFVIVISNPLDAMVTLCQKVTGFPYNRVIGQAGVLDSARFATFIAWE
LGVSVKDVTAMTLGGHGDDMVPLVRYASVKGIPVMELLERKYGSKEKAKEVMDAMVNRTRLAGGEVVALLKTGSAFYSPA
SSAIAMAESILKDQKRVLPTCAYLQGEFGVNGYYVGVPCVLGEKGIENIIEFSLDAEEQAMMDKSVAAVKSLVDSLK
>Mature_316_residues
ARKKIALIGGGQIGGVLAQLCALRELGDVVLFDIVEGLPQGKCLDIAEASPVDGFDVCLKGTNSYEDIAGADVVIVTAGL
PRKPGMSRDDLIEVNSKIMTSVAEGIKQYAPNSFVIVISNPLDAMVTLCQKVTGFPYNRVIGQAGVLDSARFATFIAWEL
GVSVKDVTAMTLGGHGDDMVPLVRYASVKGIPVMELLERKYGSKEKAKEVMDAMVNRTRLAGGEVVALLKTGSAFYSPAS
SAIAMAESILKDQKRVLPTCAYLQGEFGVNGYYVGVPCVLGEKGIENIIEFSLDAEEQAMMDKSVAAVKSLVDSLK

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=319, Percent_Identity=32.6018808777429, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI221136809, Length=319, Percent_Identity=32.6018808777429, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI15082234, Length=319, Percent_Identity=30.4075235109718, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI5031857, Length=308, Percent_Identity=31.8181818181818, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI260099723, Length=308, Percent_Identity=31.8181818181818, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI9257228, Length=306, Percent_Identity=31.0457516339869, Blast_Score=148, Evalue=6e-36,
Organism=Homo sapiens, GI4504973, Length=306, Percent_Identity=31.0457516339869, Blast_Score=148, Evalue=6e-36,
Organism=Homo sapiens, GI291575128, Length=326, Percent_Identity=29.7546012269939, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI4557032, Length=326, Percent_Identity=29.7546012269939, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI260099727, Length=220, Percent_Identity=32.2727272727273, Blast_Score=111, Evalue=8e-25,
Organism=Homo sapiens, GI260099725, Length=212, Percent_Identity=32.0754716981132, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI207028494, Length=192, Percent_Identity=33.8541666666667, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI21735621, Length=254, Percent_Identity=27.5590551181102, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI103472011, Length=317, Percent_Identity=23.3438485804416, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1789632, Length=327, Percent_Identity=29.0519877675841, Blast_Score=95, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17535107, Length=289, Percent_Identity=24.5674740484429, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17554310, Length=313, Percent_Identity=28.7539936102236, Blast_Score=98, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6322765, Length=304, Percent_Identity=28.2894736842105, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320125, Length=257, Percent_Identity=29.1828793774319, Blast_Score=83, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324446, Length=201, Percent_Identity=29.8507462686567, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17136226, Length=316, Percent_Identity=27.2151898734177, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24647881, Length=312, Percent_Identity=28.8461538461538, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI45550422, Length=305, Percent_Identity=27.5409836065574, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24663599, Length=242, Percent_Identity=29.7520661157025, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24663595, Length=241, Percent_Identity=26.5560165975104, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 33677; Mature: 33545

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKKIALIGGGQIGGVLAQLCALRELGDVVLFDIVEGLPQGKCLDIAEASPVDGFDVCL
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHEE
KGTNSYEDIAGADVVIVTAGLPRKPGMSRDDLIEVNSKIMTSVAEGIKQYAPNSFVIVIS
ECCCCCHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
NPLDAMVTLCQKVTGFPYNRVIGQAGVLDSARFATFIAWELGVSVKDVTAMTLGGHGDDM
CCHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHEEEEEECCCCCCH
VPLVRYASVKGIPVMELLERKYGSKEKAKEVMDAMVNRTRLAGGEVVALLKTGSAFYSPA
HHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHCCH
SSAIAMAESILKDQKRVLPTCAYLQGEFGVNGYYVGVPCVLGEKGIENIIEFSLDAEEQA
HHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCEEEECCEEECHHHHHHHHHHCCCCHHHH
MMDKSVAAVKSLVDSLK
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARKKIALIGGGQIGGVLAQLCALRELGDVVLFDIVEGLPQGKCLDIAEASPVDGFDVCL
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHEE
KGTNSYEDIAGADVVIVTAGLPRKPGMSRDDLIEVNSKIMTSVAEGIKQYAPNSFVIVIS
ECCCCCHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
NPLDAMVTLCQKVTGFPYNRVIGQAGVLDSARFATFIAWELGVSVKDVTAMTLGGHGDDM
CCHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHEEEEEECCCCCCH
VPLVRYASVKGIPVMELLERKYGSKEKAKEVMDAMVNRTRLAGGEVVALLKTGSAFYSPA
HHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHCCH
SSAIAMAESILKDQKRVLPTCAYLQGEFGVNGYYVGVPCVLGEKGIENIIEFSLDAEEQA
HHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCEEEECCEEECHHHHHHHHHHCCCCHHHH
MMDKSVAAVKSLVDSLK
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA