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The map label for this gene is aspS

Identifier: 39996563

GI number: 39996563

Start: 1603748

End: 1605532

Strand: Direct

Name: aspS

Synonym: GSU1463

Alternate gene names: 39996563

Gene position: 1603748-1605532 (Clockwise)

Preceding gene: 39996562

Following gene: 39996564

Centisome position: 42.05

GC content: 56.36

Gene sequence:

>1785_bases
ATGACCGATATGCTTGGCGACTGGAAACGATCTCACCTCTGCGGAACCTTGACCAAAGCCGATGTGGGCAAGCAGGTGAC
GCTCATGGGCTGGGTGATGCGCCGTCGTGACCACGGCGGACTCATTTTTATCGATCTTCGCGACCGGGAAGGGCTTGCCC
AGATTGTTTTCGATCCCGCCAAGGCGCCAGAGGCCCATCGGGAGGCCGAGGCCGTCCGCAACGAATACGTCGTGGCCATT
AAGGGTGAGGTTGTTCCGCGCCCCGAGGGGACCGTCAATCCGAACATGAAAACGGGAGAAGTGGAGATTCTCGTCACCCA
GTGCAAGTTGTTGAACCGTTCCAAGGCGCTTCCCTTTACGCTTGATGATTATGTCGATGTGGCCGAGAACCTGCGCCTCA
AGCATCGTTATCTCGATCTGCGCCGTACGCCTCTTCAACAGAACCTGATTCTTCGTTCCCGGGTTAGCCAGGTTACCCGC
CAGTATCTGACCGAAAACGGTTTTCTGGAGATTGAAACTCCGTTCCTCACCAAGTCGACCCCCGAAGGCGCCAGGGACTT
CCTCGTGCCGTCCCGGATCAATCAAGGCAACTTTTACGCTCTCCCTCAATCGCCCCAGATATTTAAGCAGATTCTCATGA
TTTCGGGTTTTGATCGCTACTTCCAGGTCGTTCGCTGCTTCCGGGACGAAGACCTGCGGGCCGATCGCCAGCCGGAGTTT
ACGCAAATCGACTGTGAGCTTTCCTTTGTTGATCGCGATGACGTTATTGCCGTCATGGAAGGCTTGATCGCGCGAATTTT
CAAGGAGGCAAAGGAGATTGACGTTCAGCTTCCGATCCCGCGCATGACGTATGCCGAGGCGATCCGCCGCTACGGCGTCG
ATAACCCCGATGTCCGTTTTGGCCTTGAATTGGTGGAGCTGACAGACATCGTCAAAGGATCCGGCTTCAAGGTTTTCGCC
GATGTGGCCGCCGGCGGGGGTATCATCAAGGGGCTCAACGCCAAGGGCTGCGCACGCTTCTCCCGCAAGGAGATCGACGA
CCTTACCGAATTCGTCAAGATTTATGGAGCCAAGGGGCTCGCGTATGTCAAGATCGAGGGAGGAGAATGGCACTCTCCCA
TAGCCAAGTTCTTTACCGCCCAAGAGATTGCCGATATGAATAGGGCTTTCGGAGCCGAGGAAGGCGACCTTCTCCTTTTT
GTGGCCGACAAGCCGAAGGTCGTCAATGACTCGCTGGGCAAGCTCCGCAATCAACTGGCCCAGATTCTCGGACTCGTCGA
CAAGGGGACCTTCAAATTTGTCTGGATCACTGACTTCCCGTTGCTGGAGTGGGACGAGGAGGAGAAGCGTTGGGCCGCTG
TCCATCACCCCTTCACCGCACCCATGGACGAAGATCTCGACAAGGTCGAGTCAGACCCCGGGGCCTGCCGTGCCAAGGCC
TACGACCTGGTTCTCAATGGTAACGAGATCGGTGGCGGTAGTATCAGGATTCATCAACAGCACATCCAGTCACTGATGTT
CAGGATGCTCGGCCTGTCCGAGGAAGAGGCCCGCGCCAAATTCGGATTCCTTCTGGATGCTCTGGAATTCGGCACACCTC
CGCACGGAGGCATTGCCTTCGGCATGGATCGACTCATAATGCTTCTTACTGGCAGTGACTCCATCCGAGACGTTATTGCG
TTCCCCAAAACGCAGAAGGGTGCTTGTCTCATGTCCGACGCCCCGTCGCCGGTTGATTCTAAGCAGCTCAGGGAGTTGGC
CATCAAGGTGACGGTCAAGCAGTGA

Upstream 100 bases:

>100_bases
TTTCCCTTGTGCGTCGGCAGTCTATTATTCCTTGAAGTAGATTTGCGCTTTCAGTATACTTGACCGGTTTTTTAAGTGAC
GAGACAGGAGGTTTTTACCG

Downstream 100 bases:

>100_bases
TCGGGGATTCCCTATGAAGGTCCGTGCCGGCATTTCCGCACTTGTGACCATTGCGGTTCTTGCGGGGTGCGCCTCACACC
TGCCGGTCTACCGGATTGAT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 594; Mature: 593

Protein sequence:

>594_residues
MTDMLGDWKRSHLCGTLTKADVGKQVTLMGWVMRRRDHGGLIFIDLRDREGLAQIVFDPAKAPEAHREAEAVRNEYVVAI
KGEVVPRPEGTVNPNMKTGEVEILVTQCKLLNRSKALPFTLDDYVDVAENLRLKHRYLDLRRTPLQQNLILRSRVSQVTR
QYLTENGFLEIETPFLTKSTPEGARDFLVPSRINQGNFYALPQSPQIFKQILMISGFDRYFQVVRCFRDEDLRADRQPEF
TQIDCELSFVDRDDVIAVMEGLIARIFKEAKEIDVQLPIPRMTYAEAIRRYGVDNPDVRFGLELVELTDIVKGSGFKVFA
DVAAGGGIIKGLNAKGCARFSRKEIDDLTEFVKIYGAKGLAYVKIEGGEWHSPIAKFFTAQEIADMNRAFGAEEGDLLLF
VADKPKVVNDSLGKLRNQLAQILGLVDKGTFKFVWITDFPLLEWDEEEKRWAAVHHPFTAPMDEDLDKVESDPGACRAKA
YDLVLNGNEIGGGSIRIHQQHIQSLMFRMLGLSEEEARAKFGFLLDALEFGTPPHGGIAFGMDRLIMLLTGSDSIRDVIA
FPKTQKGACLMSDAPSPVDSKQLRELAIKVTVKQ

Sequences:

>Translated_594_residues
MTDMLGDWKRSHLCGTLTKADVGKQVTLMGWVMRRRDHGGLIFIDLRDREGLAQIVFDPAKAPEAHREAEAVRNEYVVAI
KGEVVPRPEGTVNPNMKTGEVEILVTQCKLLNRSKALPFTLDDYVDVAENLRLKHRYLDLRRTPLQQNLILRSRVSQVTR
QYLTENGFLEIETPFLTKSTPEGARDFLVPSRINQGNFYALPQSPQIFKQILMISGFDRYFQVVRCFRDEDLRADRQPEF
TQIDCELSFVDRDDVIAVMEGLIARIFKEAKEIDVQLPIPRMTYAEAIRRYGVDNPDVRFGLELVELTDIVKGSGFKVFA
DVAAGGGIIKGLNAKGCARFSRKEIDDLTEFVKIYGAKGLAYVKIEGGEWHSPIAKFFTAQEIADMNRAFGAEEGDLLLF
VADKPKVVNDSLGKLRNQLAQILGLVDKGTFKFVWITDFPLLEWDEEEKRWAAVHHPFTAPMDEDLDKVESDPGACRAKA
YDLVLNGNEIGGGSIRIHQQHIQSLMFRMLGLSEEEARAKFGFLLDALEFGTPPHGGIAFGMDRLIMLLTGSDSIRDVIA
FPKTQKGACLMSDAPSPVDSKQLRELAIKVTVKQ
>Mature_593_residues
TDMLGDWKRSHLCGTLTKADVGKQVTLMGWVMRRRDHGGLIFIDLRDREGLAQIVFDPAKAPEAHREAEAVRNEYVVAIK
GEVVPRPEGTVNPNMKTGEVEILVTQCKLLNRSKALPFTLDDYVDVAENLRLKHRYLDLRRTPLQQNLILRSRVSQVTRQ
YLTENGFLEIETPFLTKSTPEGARDFLVPSRINQGNFYALPQSPQIFKQILMISGFDRYFQVVRCFRDEDLRADRQPEFT
QIDCELSFVDRDDVIAVMEGLIARIFKEAKEIDVQLPIPRMTYAEAIRRYGVDNPDVRFGLELVELTDIVKGSGFKVFAD
VAAGGGIIKGLNAKGCARFSRKEIDDLTEFVKIYGAKGLAYVKIEGGEWHSPIAKFFTAQEIADMNRAFGAEEGDLLLFV
ADKPKVVNDSLGKLRNQLAQILGLVDKGTFKFVWITDFPLLEWDEEEKRWAAVHHPFTAPMDEDLDKVESDPGACRAKAY
DLVLNGNEIGGGSIRIHQQHIQSLMFRMLGLSEEEARAKFGFLLDALEFGTPPHGGIAFGMDRLIMLLTGSDSIRDVIAF
PKTQKGACLMSDAPSPVDSKQLRELAIKVTVKQ

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=594, Percent_Identity=43.2659932659933, Blast_Score=462, Evalue=1e-130,
Organism=Homo sapiens, GI5031815, Length=281, Percent_Identity=28.4697508896797, Blast_Score=89, Evalue=2e-17,
Organism=Homo sapiens, GI194272210, Length=273, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI45439306, Length=323, Percent_Identity=26.9349845201238, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1788173, Length=590, Percent_Identity=51.5254237288136, Blast_Score=605, Evalue=1e-174,
Organism=Escherichia coli, GI1790571, Length=310, Percent_Identity=26.1290322580645, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789256, Length=318, Percent_Identity=27.6729559748428, Blast_Score=99, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI32566633, Length=598, Percent_Identity=36.1204013377926, Blast_Score=337, Evalue=8e-93,
Organism=Caenorhabditis elegans, GI71994340, Length=143, Percent_Identity=32.1678321678322, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17535927, Length=143, Percent_Identity=32.1678321678322, Blast_Score=86, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17535925, Length=143, Percent_Identity=32.1678321678322, Blast_Score=85, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6325153, Length=631, Percent_Identity=32.3296354992076, Blast_Score=285, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6321807, Length=270, Percent_Identity=27.037037037037, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323011, Length=307, Percent_Identity=27.0358306188925, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324256, Length=173, Percent_Identity=26.0115606936416, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320242, Length=173, Percent_Identity=26.5895953757225, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24584738, Length=602, Percent_Identity=40.3654485049834, Blast_Score=397, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24640849, Length=251, Percent_Identity=27.0916334661355, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24640851, Length=251, Percent_Identity=27.0916334661355, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI17136276, Length=278, Percent_Identity=28.0575539568345, Blast_Score=80, Evalue=5e-15,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_GEOSL (Q74D56)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_952514.1
- ProteinModelPortal:   Q74D56
- SMR:   Q74D56
- GeneID:   2685860
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU1463
- NMPDR:   fig|243231.1.peg.1452
- TIGR:   GSU1463
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- BioCyc:   GSUL243231:GSU_1463-MONOMER
- BRENDA:   6.1.1.12
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66826; Mature: 66695

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDMLGDWKRSHLCGTLTKADVGKQVTLMGWVMRRRDHGGLIFIDLRDREGLAQIVFDPA
CCCCCCCHHHHHHHCCCHHHCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHEECCC
KAPEAHREAEAVRNEYVVAIKGEVVPRPEGTVNPNMKTGEVEILVTQCKLLNRSKALPFT
CCCHHHHHHHHHCCCEEEEEECCEECCCCCCCCCCCCCCCEEEEEEEHHHHCCCCCCCEE
LDDYVDVAENLRLKHRYLDLRRTPLQQNLILRSRVSQVTRQYLTENGFLEIETPFLTKST
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
PEGARDFLVPSRINQGNFYALPQSPQIFKQILMISGFDRYFQVVRCFRDEDLRADRQPEF
CCCHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCC
TQIDCELSFVDRDDVIAVMEGLIARIFKEAKEIDVQLPIPRMTYAEAIRRYGVDNPDVRF
EEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCEEECCCCCHHHHHHHHHCCCCCCCHHH
GLELVELTDIVKGSGFKVFADVAAGGGIIKGLNAKGCARFSRKEIDDLTEFVKIYGAKGL
HHHHHHHHHHHCCCCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCE
AYVKIEGGEWHSPIAKFFTAQEIADMNRAFGAEEGDLLLFVADKPKVVNDSLGKLRNQLA
EEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
QILGLVDKGTFKFVWITDFPLLEWDEEEKRWAAVHHPFTAPMDEDLDKVESDPGACRAKA
HHHHHHCCCCEEEEEEECCCCCCCCCHHHHEEHHCCCCCCCCHHHHHHHCCCCCCCEEEE
YDLVLNGNEIGGGSIRIHQQHIQSLMFRMLGLSEEEARAKFGFLLDALEFGTPPHGGIAF
EEEEEECCEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHH
GMDRLIMLLTGSDSIRDVIAFPKTQKGACLMSDAPSPVDSKQLRELAIKVTVKQ
CCCEEEEEEECCCHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHEEEEEEECC
>Mature Secondary Structure 
TDMLGDWKRSHLCGTLTKADVGKQVTLMGWVMRRRDHGGLIFIDLRDREGLAQIVFDPA
CCCCCCHHHHHHHCCCHHHCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHEECCC
KAPEAHREAEAVRNEYVVAIKGEVVPRPEGTVNPNMKTGEVEILVTQCKLLNRSKALPFT
CCCHHHHHHHHHCCCEEEEEECCEECCCCCCCCCCCCCCCEEEEEEEHHHHCCCCCCCEE
LDDYVDVAENLRLKHRYLDLRRTPLQQNLILRSRVSQVTRQYLTENGFLEIETPFLTKST
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
PEGARDFLVPSRINQGNFYALPQSPQIFKQILMISGFDRYFQVVRCFRDEDLRADRQPEF
CCCHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCC
TQIDCELSFVDRDDVIAVMEGLIARIFKEAKEIDVQLPIPRMTYAEAIRRYGVDNPDVRF
EEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCEEECCCCCHHHHHHHHHCCCCCCCHHH
GLELVELTDIVKGSGFKVFADVAAGGGIIKGLNAKGCARFSRKEIDDLTEFVKIYGAKGL
HHHHHHHHHHHCCCCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCE
AYVKIEGGEWHSPIAKFFTAQEIADMNRAFGAEEGDLLLFVADKPKVVNDSLGKLRNQLA
EEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
QILGLVDKGTFKFVWITDFPLLEWDEEEKRWAAVHHPFTAPMDEDLDKVESDPGACRAKA
HHHHHHCCCCEEEEEEECCCCCCCCCHHHHEEHHCCCCCCCCHHHHHHHCCCCCCCEEEE
YDLVLNGNEIGGGSIRIHQQHIQSLMFRMLGLSEEEARAKFGFLLDALEFGTPPHGGIAF
EEEEEECCEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHH
GMDRLIMLLTGSDSIRDVIAFPKTQKGACLMSDAPSPVDSKQLRELAIKVTVKQ
CCCEEEEEEECCCHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA