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The map label for this gene is proS

Identifier: 39996560

GI number: 39996560

Start: 1599970

End: 1601682

Strand: Direct

Name: proS

Synonym: GSU1460

Alternate gene names: 39996560

Gene position: 1599970-1601682 (Clockwise)

Preceding gene: 39996559

Following gene: 39996561

Centisome position: 41.95

GC content: 61.76

Gene sequence:

>1713_bases
ATGCGTTACACCAACTACTTCATCCCCACCCTCAAGGAAACCCCGTCCGACGCCGAGGTGGTCTCTCACCAGCTCATGCT
CCGGGCCGGTATGATCCGTAAACTTGCCGCGGGCATCTACAATTATCTGCCGTTGGGACTGCGCTCCATCCGCAAAGTGG
AAACAATCGTGCGCGAGGAGATGGACCGCGCCGGCGCCATCGAGCTGCTGATGCCGAGCGTACAACCGGCCGAGCTCTGG
CAGGAATCCACCCGGTGGGAACAGTACGGCAAGGAACTTTTGCGCTTCAAGGACCGCAAGGATGCGGAATTCTGTCTCGG
ACCCACCCATGAGGAAGTGGTCACCGACATTGTCCGCCGTGAAGTGAAAAGCTACCGGCAGATGCCCTTCAACCTCTACC
AGATCCAGGCCAAGTTCCGGGACGAGATCCGCCCCCGGTTCGGCCTCATGCGGGGGCGCGAGTTCATCATGAAGGACGCC
TATTCCTTCGACGTCGACAGCTCCGCTTCGGATCTTTCCTATGACAAGATGTACCAGGCCTATCGCCGGATTTTCCAGCG
CTGCGGCCTCAAATTCAGGGCAGTGGAGGCAGATACCGGCTCCATCGGCGGTTCGTCGTCCCACGAATTCATGGTGCTTG
CCGATTCGGGCGAGGATGCCATTGTTTCATGCACCCAGTGCGAGTATGCCGCCAACGTGGAAAAGGCCGAGGCCCGACCC
GCCCCTGCCGAGCATGCCGAGCCGCGCCCTCTGGAAAAGGTGGAGACACCGGCCAAGCGGAGTGTCGAAGAGGTAACGGC
CTTCCTCGGCATCCCCTCGTCCGCCCTGGTGAAAACGCTGCTGGTAGTGGCCGACGGTACACCCGTGGCCGCCCTCGTGC
GCGGCGATCACGACCTGAACGAGATCAAGCTCAAACATTTGTTGGGTTGCGAGACCCTGGAGATGGCCAACGAGGAAATG
GTGACCAGGGTAACCGGCGCTCCCGTGGGCTTTGCCGGCCCGGTGGGGCTGAACATCAAGATCGTTGCCGATCTGGCCGT
CCAGGGGATGAAGAACTTCGTTACCGGAGCCAATGCCGGCGATCTGCACCTGAAAAACGTCACCATCGGCCGCGACTTTA
CCCCGACTCAGTATGCCGACATCCGCAACGTGGTCCACGGTGACCCCTGTCCCCGTTGCGAGGCGGGACACCTCGAACTG
TGGCGAGGCATCGAGGTGGGGCACGTCTTCAAGCTGGGCACCAAGTATTCGGAGGCCCTGCGCGCCACCTACCTGGACGC
CGACGGCAAGGAGCAGATCATCTTCATGGGGTGCTACGGTATTGGCATCAGCCGGACCGTGGCCGCCTGCATCGAGCAGA
ACCATGATGCCGACGGCATCATCTTCCCCATTCCCATTGCCCCGTTCCACTGCATCGTCTCGGCGGTCAACACCAAGGAC
GCAGAGGTCATGGCCGCCTGTGAGTCACTCTATCTCGACCTGCGCGCCGCAGGGGTGGAGGTGCTCTTCGACGATCGCGA
CGAGCGCCCAGGCATCAAGTTCAAGGACGCCGATCTCATCGGCATACCCCTCCGTTTGGTGGTCGGCTCGAAGAATCTGG
CCGACGGCAAGGTGGAGCTCAAGAGCCGCAGGACCGGCGAGGTGGAACTGCTTCCGCTGGCCGGCGCGGTGGAAAAGGTC
CGGAACATCGTGGCCGAAGCCCTCGGCCGGTAA

Upstream 100 bases:

>100_bases
GAAATCGTCCGCAAGGTGCCGGAGGCCGACATGGCCGATGCGCTGATTGCGGAAGTCGAAAAGATACTCGCTGAAAAACA
CTAATTTCCGAGGTTAGTCG

Downstream 100 bases:

>100_bases
CCGTACAAGGAGATATCACCGTTATGACAAGAGATCAAGCGCGGGAGAAGGTTATCTTCGCCCTCGATTCCAGTGAATTC
GCCCATGTCCAGTACTGGGC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MRYTNYFIPTLKETPSDAEVVSHQLMLRAGMIRKLAAGIYNYLPLGLRSIRKVETIVREEMDRAGAIELLMPSVQPAELW
QESTRWEQYGKELLRFKDRKDAEFCLGPTHEEVVTDIVRREVKSYRQMPFNLYQIQAKFRDEIRPRFGLMRGREFIMKDA
YSFDVDSSASDLSYDKMYQAYRRIFQRCGLKFRAVEADTGSIGGSSSHEFMVLADSGEDAIVSCTQCEYAANVEKAEARP
APAEHAEPRPLEKVETPAKRSVEEVTAFLGIPSSALVKTLLVVADGTPVAALVRGDHDLNEIKLKHLLGCETLEMANEEM
VTRVTGAPVGFAGPVGLNIKIVADLAVQGMKNFVTGANAGDLHLKNVTIGRDFTPTQYADIRNVVHGDPCPRCEAGHLEL
WRGIEVGHVFKLGTKYSEALRATYLDADGKEQIIFMGCYGIGISRTVAACIEQNHDADGIIFPIPIAPFHCIVSAVNTKD
AEVMAACESLYLDLRAAGVEVLFDDRDERPGIKFKDADLIGIPLRLVVGSKNLADGKVELKSRRTGEVELLPLAGAVEKV
RNIVAEALGR

Sequences:

>Translated_570_residues
MRYTNYFIPTLKETPSDAEVVSHQLMLRAGMIRKLAAGIYNYLPLGLRSIRKVETIVREEMDRAGAIELLMPSVQPAELW
QESTRWEQYGKELLRFKDRKDAEFCLGPTHEEVVTDIVRREVKSYRQMPFNLYQIQAKFRDEIRPRFGLMRGREFIMKDA
YSFDVDSSASDLSYDKMYQAYRRIFQRCGLKFRAVEADTGSIGGSSSHEFMVLADSGEDAIVSCTQCEYAANVEKAEARP
APAEHAEPRPLEKVETPAKRSVEEVTAFLGIPSSALVKTLLVVADGTPVAALVRGDHDLNEIKLKHLLGCETLEMANEEM
VTRVTGAPVGFAGPVGLNIKIVADLAVQGMKNFVTGANAGDLHLKNVTIGRDFTPTQYADIRNVVHGDPCPRCEAGHLEL
WRGIEVGHVFKLGTKYSEALRATYLDADGKEQIIFMGCYGIGISRTVAACIEQNHDADGIIFPIPIAPFHCIVSAVNTKD
AEVMAACESLYLDLRAAGVEVLFDDRDERPGIKFKDADLIGIPLRLVVGSKNLADGKVELKSRRTGEVELLPLAGAVEKV
RNIVAEALGR
>Mature_570_residues
MRYTNYFIPTLKETPSDAEVVSHQLMLRAGMIRKLAAGIYNYLPLGLRSIRKVETIVREEMDRAGAIELLMPSVQPAELW
QESTRWEQYGKELLRFKDRKDAEFCLGPTHEEVVTDIVRREVKSYRQMPFNLYQIQAKFRDEIRPRFGLMRGREFIMKDA
YSFDVDSSASDLSYDKMYQAYRRIFQRCGLKFRAVEADTGSIGGSSSHEFMVLADSGEDAIVSCTQCEYAANVEKAEARP
APAEHAEPRPLEKVETPAKRSVEEVTAFLGIPSSALVKTLLVVADGTPVAALVRGDHDLNEIKLKHLLGCETLEMANEEM
VTRVTGAPVGFAGPVGLNIKIVADLAVQGMKNFVTGANAGDLHLKNVTIGRDFTPTQYADIRNVVHGDPCPRCEAGHLEL
WRGIEVGHVFKLGTKYSEALRATYLDADGKEQIIFMGCYGIGISRTVAACIEQNHDADGIIFPIPIAPFHCIVSAVNTKD
AEVMAACESLYLDLRAAGVEVLFDDRDERPGIKFKDADLIGIPLRLVVGSKNLADGKVELKSRRTGEVELLPLAGAVEKV
RNIVAEALGR

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=226, Percent_Identity=46.4601769911504, Blast_Score=229, Evalue=6e-60,
Organism=Escherichia coli, GI1786392, Length=565, Percent_Identity=54.5132743362832, Blast_Score=605, Evalue=1e-174,
Organism=Caenorhabditis elegans, GI115532348, Length=252, Percent_Identity=37.6984126984127, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI193203271, Length=89, Percent_Identity=37.0786516853933, Blast_Score=76, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320931, Length=558, Percent_Identity=31.0035842293907, Blast_Score=266, Evalue=8e-72,
Organism=Drosophila melanogaster, GI24656200, Length=276, Percent_Identity=35.8695652173913, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24649466, Length=168, Percent_Identity=32.1428571428571, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24649468, Length=168, Percent_Identity=32.1428571428571, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_GEOSL (Q74D59)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_952511.1
- ProteinModelPortal:   Q74D59
- SMR:   Q74D59
- GeneID:   2686217
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU1460
- NMPDR:   fig|243231.1.peg.1449
- TIGR:   GSU1460
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- BioCyc:   GSUL243231:GSU_1460-MONOMER
- BRENDA:   6.1.1.15
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 63154; Mature: 63154

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYTNYFIPTLKETPSDAEVVSHQLMLRAGMIRKLAAGIYNYLPLGLRSIRKVETIVREE
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDRAGAIELLMPSVQPAELWQESTRWEQYGKELLRFKDRKDAEFCLGPTHEEVVTDIVRR
HHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHH
EVKSYRQMPFNLYQIQAKFRDEIRPRFGLMRGREFIMKDAYSFDVDSSASDLSYDKMYQA
HHHHHHCCCCEEEHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
YRRIFQRCGLKFRAVEADTGSIGGSSSHEFMVLADSGEDAIVSCTQCEYAANVEKAEARP
HHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEEECCHHHCCCHHHCCCC
APAEHAEPRPLEKVETPAKRSVEEVTAFLGIPSSALVKTLLVVADGTPVAALVRGDHDLN
CCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHEECCCCEEEEEECCCCHH
EIKLKHLLGCETLEMANEEMVTRVTGAPVGFAGPVGLNIKIVADLAVQGMKNFVTGANAG
HHHHHHHHCCHHHHHCCHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCC
DLHLKNVTIGRDFTPTQYADIRNVVHGDPCPRCEAGHLELWRGIEVGHVFKLGTKYSEAL
CEEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCEEECCCHHHHHH
RATYLDADGKEQIIFMGCYGIGISRTVAACIEQNHDADGIIFPIPIAPFHCIVSAVNTKD
HHHEECCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHCCCCC
AEVMAACESLYLDLRAAGVEVLFDDRDERPGIKFKDADLIGIPLRLVVGSKNLADGKVEL
HHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEECCCCEECCCEEEEECCCCCCCCEEEE
KSRRTGEVELLPLAGAVEKVRNIVAEALGR
CCCCCCCEEEEECHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRYTNYFIPTLKETPSDAEVVSHQLMLRAGMIRKLAAGIYNYLPLGLRSIRKVETIVREE
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDRAGAIELLMPSVQPAELWQESTRWEQYGKELLRFKDRKDAEFCLGPTHEEVVTDIVRR
HHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHH
EVKSYRQMPFNLYQIQAKFRDEIRPRFGLMRGREFIMKDAYSFDVDSSASDLSYDKMYQA
HHHHHHCCCCEEEHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
YRRIFQRCGLKFRAVEADTGSIGGSSSHEFMVLADSGEDAIVSCTQCEYAANVEKAEARP
HHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEEECCHHHCCCHHHCCCC
APAEHAEPRPLEKVETPAKRSVEEVTAFLGIPSSALVKTLLVVADGTPVAALVRGDHDLN
CCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHEECCCCEEEEEECCCCHH
EIKLKHLLGCETLEMANEEMVTRVTGAPVGFAGPVGLNIKIVADLAVQGMKNFVTGANAG
HHHHHHHHCCHHHHHCCHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCC
DLHLKNVTIGRDFTPTQYADIRNVVHGDPCPRCEAGHLELWRGIEVGHVFKLGTKYSEAL
CEEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCEEECCCHHHHHH
RATYLDADGKEQIIFMGCYGIGISRTVAACIEQNHDADGIIFPIPIAPFHCIVSAVNTKD
HHHEECCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHCCCCC
AEVMAACESLYLDLRAAGVEVLFDDRDERPGIKFKDADLIGIPLRLVVGSKNLADGKVEL
HHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEECCCCEECCCEEEEECCCCCCCCEEEE
KSRRTGEVELLPLAGAVEKVRNIVAEALGR
CCCCCCCEEEEECHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA