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The map label for this gene is pyrF

Identifier: 39996561

GI number: 39996561

Start: 1601706

End: 1602425

Strand: Direct

Name: pyrF

Synonym: GSU1461

Alternate gene names: 39996561

Gene position: 1601706-1602425 (Clockwise)

Preceding gene: 39996560

Following gene: 39996562

Centisome position: 41.99

GC content: 63.19

Gene sequence:

>720_bases
ATGACAAGAGATCAAGCGCGGGAGAAGGTTATCTTCGCCCTCGATTCCAGTGAATTCGCCCATGTCCAGTACTGGGCCGA
AACCCTGTCCGACCGGGTGGGCATGTTCAAGGTCGGCAAGCAGTTGTTCACCGCCTGCGGGCCGGCCACGGTCCGGATGA
TCCAGAAATTCGGCGGAGAGGTTTTCCTGGACCTCAAGTTCCACGATATTCCCAACACCGTTGCCATGGCCTCCCTTGAG
GCAGCCCGGCTGGGAGTAAAACTCTTCAACCTCCATGCCCTGGGAGGGTACGAGATGATGGCCCGCACGGTGGAGGCGCT
GGACAAGGAGTTCAAGGGGGGGGAGAGAGCGAAGGTGCTGGCCGTTACCATCCTTACCTCCTCAACCGAAGAGACCCTGC
GCCAGGTGGGGATCGAATCGCCGGTGGAGGAGATGGTCGTGCGGCTCGCCACCCTGGCCAGGAAGGCCGGCATCGACGGC
GTGGTCGCTTCACCGCGGGAGATCCCCCTTATCCGCGAGGCATGCGGTCCCGATTTCCTCATCGTCACGCCGGGGGTACG
CCCGGCCTTCGCCGCTCTCAACGACCAGAAGCGGGTCATGACTCCTGCTGAAGCGGTCAGGGCAGGGGGGGATTACCTCG
TTATCGGCCGCCCGATCGGCGACGCCCCTGACCCGGCCGGCGCAGCCGAAATGATTCTCGACGAGATCATGGCCGGCTGA

Upstream 100 bases:

>100_bases
GCGAGGTGGAACTGCTTCCGCTGGCCGGCGCGGTGGAAAAGGTCCGGAACATCGTGGCCGAAGCCCTCGGCCGGTAACCG
TACAAGGAGATATCACCGTT

Downstream 100 bases:

>100_bases
GGGAAAAAGGAACCACAGCGTGAAAGAAGAAATTCTGTACGGAATCAACAGTGTGATGGAGGCCCTGCGCGGATCGCGGA
GGGCCTTCGAACTTTTCGTT

Product: orotidine 5`-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MTRDQAREKVIFALDSSEFAHVQYWAETLSDRVGMFKVGKQLFTACGPATVRMIQKFGGEVFLDLKFHDIPNTVAMASLE
AARLGVKLFNLHALGGYEMMARTVEALDKEFKGGERAKVLAVTILTSSTEETLRQVGIESPVEEMVVRLATLARKAGIDG
VVASPREIPLIREACGPDFLIVTPGVRPAFAALNDQKRVMTPAEAVRAGGDYLVIGRPIGDAPDPAGAAEMILDEIMAG

Sequences:

>Translated_239_residues
MTRDQAREKVIFALDSSEFAHVQYWAETLSDRVGMFKVGKQLFTACGPATVRMIQKFGGEVFLDLKFHDIPNTVAMASLE
AARLGVKLFNLHALGGYEMMARTVEALDKEFKGGERAKVLAVTILTSSTEETLRQVGIESPVEEMVVRLATLARKAGIDG
VVASPREIPLIREACGPDFLIVTPGVRPAFAALNDQKRVMTPAEAVRAGGDYLVIGRPIGDAPDPAGAAEMILDEIMAG
>Mature_238_residues
TRDQAREKVIFALDSSEFAHVQYWAETLSDRVGMFKVGKQLFTACGPATVRMIQKFGGEVFLDLKFHDIPNTVAMASLEA
ARLGVKLFNLHALGGYEMMARTVEALDKEFKGGERAKVLAVTILTSSTEETLRQVGIESPVEEMVVRLATLARKAGIDGV
VASPREIPLIREACGPDFLIVTPGVRPAFAALNDQKRVMTPAEAVRAGGDYLVIGRPIGDAPDPAGAAEMILDEIMAG

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=231, Percent_Identity=41.991341991342, Blast_Score=161, Evalue=5e-41,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_GEOSL (Q74D58)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_952512.1
- ProteinModelPortal:   Q74D58
- GeneID:   2686214
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU1461
- NMPDR:   fig|243231.1.peg.1450
- TIGR:   GSU1461
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- ProtClustDB:   CLSK828364
- BioCyc:   GSUL243231:GSU_1461-MONOMER
- BRENDA:   4.1.1.23
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 25818; Mature: 25687

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 66-66 BINDING 15-15 BINDING 37-37 BINDING 126-126 BINDING 187-187 BINDING 196-196 BINDING 216-216 BINDING 217-217

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRDQAREKVIFALDSSEFAHVQYWAETLSDRVGMFKVGKQLFTACGPATVRMIQKFGGE
CCCHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCE
VFLDLKFHDIPNTVAMASLEAARLGVKLFNLHALGGYEMMARTVEALDKEFKGGERAKVL
EEEEEEECCCCCHHHHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEE
AVTILTSSTEETLRQVGIESPVEEMVVRLATLARKAGIDGVVASPREIPLIREACGPDFL
EEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCEE
IVTPGVRPAFAALNDQKRVMTPAEAVRAGGDYLVIGRPIGDAPDPAGAAEMILDEIMAG
EECCCCCHHHHHCCCCCHHCCHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TRDQAREKVIFALDSSEFAHVQYWAETLSDRVGMFKVGKQLFTACGPATVRMIQKFGGE
CCHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCE
VFLDLKFHDIPNTVAMASLEAARLGVKLFNLHALGGYEMMARTVEALDKEFKGGERAKVL
EEEEEEECCCCCHHHHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEE
AVTILTSSTEETLRQVGIESPVEEMVVRLATLARKAGIDGVVASPREIPLIREACGPDFL
EEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCEE
IVTPGVRPAFAALNDQKRVMTPAEAVRAGGDYLVIGRPIGDAPDPAGAAEMILDEIMAG
EECCCCCHHHHHCCCCCHHCCHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA