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The map label for this gene is ispG

Identifier: 39996559

GI number: 39996559

Start: 1598892

End: 1599953

Strand: Direct

Name: ispG

Synonym: GSU1459

Alternate gene names: 39996559

Gene position: 1598892-1599953 (Clockwise)

Preceding gene: 39996558

Following gene: 39996560

Centisome position: 41.92

GC content: 61.49

Gene sequence:

>1062_bases
ATGAAAGCCAAGACGAGACAGATCCGAGTGGGGAACGTACCGGTGGGCGGCGATGCGCCCTGCTCGGTGCAATCCATGTG
CAATACCGATACGCGGGACGCGGGCGCCACCCTTGATCAGATCAACGCCCTGGCGGCTGCGGGCTGTGAAATCGTCCGCT
GCGCCGTCCCCGACATGGCCGCTGCCGAGGCCTTGGGCGCCATCAAGCGCCAAAGCCCCATCCCGGTTATTGCCGACATC
CACTTTGACTACAAACTTGCCCTGAAGGTACTGGAGGGGGGAATTGACGGCCTGCGTCTCAATCCCGGCAATATCGGCGA
ACGGTGGAAGGTGGAAGAGGTTGTAGCAGCGGCCCGGGAACGGCTGGTACCCATCCGCATCGGCGTCAATGCCGGCTCTC
TAGAAAAGGAGCTTCTTCAGAAATACGGCCACCCGACCGCCGAGGCCATGGTCGAGTCGGCCCTTGGCCATGTGCGTATT
CTCGAAGAACTCGGCTACGATCAGATCAAGATATCTCTCAAGGCATCGGACGTACCCAAGACGGTGGCGGCTTACCGGCT
TCTGGCCCAACGGATCGACTATCCGCTTCACATCGGCATTACCGAAGCGGGCACTATGTTCTCCGGAACCATCAAGTCAG
CCGTGGGGCTTGGCATCCTGCTTGCCGACGGCATCGGCGATACGCTCCGGGTATCCCTCACGGGTGATCCGGTGGACGAG
GTGCGGGTCGGCTTCGAGATCCTCAAGGCGCTTAATCTCAGACAAAAAGGGATTAATCTTGTCTCCTGCCCCACCTGCGG
CCGTTGCCAGATCAACCTCATCGGGGTGGCCGAAGAGGTTGAGAAGCGTCTCGCCGGCATCGACGCCCATCTCACCGTGG
CGGTCATGGGATGCGTCGTGAACGGACCCGGCGAGGCCCGCGAGGCCGACGTGGGGATTGCCGGCGGACGGGGCGAGGGG
CTCCTGTTCCGCAACGGGGAAATCGTCCGCAAGGTGCCGGAGGCCGACATGGCCGATGCGCTGATTGCGGAAGTCGAAAA
GATACTCGCTGAAAAACACTAA

Upstream 100 bases:

>100_bases
TATTCCTTCTGATCAGCCGGCTGAACATGCCCCGTTCGGACCTCCGGACGGGGCACGATTTCTTGACTTTTCGCCGAATG
TCCGGCTACTATGCCCCGTT

Downstream 100 bases:

>100_bases
TTTCCGAGGTTAGTCGATGCGTTACACCAACTACTTCATCCCCACCCTCAAGGAAACCCCGTCCGACGCCGAGGTGGTCT
CTCACCAGCTCATGCTCCGG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MKAKTRQIRVGNVPVGGDAPCSVQSMCNTDTRDAGATLDQINALAAAGCEIVRCAVPDMAAAEALGAIKRQSPIPVIADI
HFDYKLALKVLEGGIDGLRLNPGNIGERWKVEEVVAAARERLVPIRIGVNAGSLEKELLQKYGHPTAEAMVESALGHVRI
LEELGYDQIKISLKASDVPKTVAAYRLLAQRIDYPLHIGITEAGTMFSGTIKSAVGLGILLADGIGDTLRVSLTGDPVDE
VRVGFEILKALNLRQKGINLVSCPTCGRCQINLIGVAEEVEKRLAGIDAHLTVAVMGCVVNGPGEAREADVGIAGGRGEG
LLFRNGEIVRKVPEADMADALIAEVEKILAEKH

Sequences:

>Translated_353_residues
MKAKTRQIRVGNVPVGGDAPCSVQSMCNTDTRDAGATLDQINALAAAGCEIVRCAVPDMAAAEALGAIKRQSPIPVIADI
HFDYKLALKVLEGGIDGLRLNPGNIGERWKVEEVVAAARERLVPIRIGVNAGSLEKELLQKYGHPTAEAMVESALGHVRI
LEELGYDQIKISLKASDVPKTVAAYRLLAQRIDYPLHIGITEAGTMFSGTIKSAVGLGILLADGIGDTLRVSLTGDPVDE
VRVGFEILKALNLRQKGINLVSCPTCGRCQINLIGVAEEVEKRLAGIDAHLTVAVMGCVVNGPGEAREADVGIAGGRGEG
LLFRNGEIVRKVPEADMADALIAEVEKILAEKH
>Mature_353_residues
MKAKTRQIRVGNVPVGGDAPCSVQSMCNTDTRDAGATLDQINALAAAGCEIVRCAVPDMAAAEALGAIKRQSPIPVIADI
HFDYKLALKVLEGGIDGLRLNPGNIGERWKVEEVVAAARERLVPIRIGVNAGSLEKELLQKYGHPTAEAMVESALGHVRI
LEELGYDQIKISLKASDVPKTVAAYRLLAQRIDYPLHIGITEAGTMFSGTIKSAVGLGILLADGIGDTLRVSLTGDPVDE
VRVGFEILKALNLRQKGINLVSCPTCGRCQINLIGVAEEVEKRLAGIDAHLTVAVMGCVVNGPGEAREADVGIAGGRGEG
LLFRNGEIVRKVPEADMADALIAEVEKILAEKH

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=344, Percent_Identity=53.7790697674419, Blast_Score=380, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_GEOSL (Q74D60)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_952510.1
- ProteinModelPortal:   Q74D60
- GeneID:   2686219
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU1459
- NMPDR:   fig|243231.1.peg.1448
- TIGR:   GSU1459
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- ProtClustDB:   PRK00366
- BioCyc:   GSUL243231:GSU_1459-MONOMER
- BRENDA:   1.17.4.3
- HAMAP:   MF_00159
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51717 DHP_synth_like

EC number: =1.17.7.1

Molecular weight: Translated: 37370; Mature: 37370

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAKTRQIRVGNVPVGGDAPCSVQSMCNTDTRDAGATLDQINALAAAGCEIVRCAVPDMA
CCCCCCEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCHH
AAEALGAIKRQSPIPVIADIHFDYKLALKVLEGGIDGLRLNPGNIGERWKVEEVVAAARE
HHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHH
RLVPIRIGVNAGSLEKELLQKYGHPTAEAMVESALGHVRILEELGYDQIKISLKASDVPK
CEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCH
TVAAYRLLAQRIDYPLHIGITEAGTMFSGTIKSAVGLGILLADGIGDTLRVSLTGDPVDE
HHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCEEEEECCCCCEEEEEECCCCHHH
VRVGFEILKALNLRQKGINLVSCPTCGRCQINLIGVAEEVEKRLAGIDAHLTVAVMGCVV
HHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEEECHHHHHHHHHCCCHHHHHHHEEHHC
NGPGEAREADVGIAGGRGEGLLFRNGEIVRKVPEADMADALIAEVEKILAEKH
CCCCCCCCCCCEEECCCCCEEEEECCHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKAKTRQIRVGNVPVGGDAPCSVQSMCNTDTRDAGATLDQINALAAAGCEIVRCAVPDMA
CCCCCCEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCHH
AAEALGAIKRQSPIPVIADIHFDYKLALKVLEGGIDGLRLNPGNIGERWKVEEVVAAARE
HHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHH
RLVPIRIGVNAGSLEKELLQKYGHPTAEAMVESALGHVRILEELGYDQIKISLKASDVPK
CEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCH
TVAAYRLLAQRIDYPLHIGITEAGTMFSGTIKSAVGLGILLADGIGDTLRVSLTGDPVDE
HHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCEEEEECCCCCEEEEEECCCCHHH
VRVGFEILKALNLRQKGINLVSCPTCGRCQINLIGVAEEVEKRLAGIDAHLTVAVMGCVV
HHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEEECHHHHHHHHHCCCHHHHHHHEEHHC
NGPGEAREADVGIAGGRGEGLLFRNGEIVRKVPEADMADALIAEVEKILAEKH
CCCCCCCCCCCEEECCCCCEEEEECCHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA