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The map label for this gene is cysP [H]

Identifier: 39996448

GI number: 39996448

Start: 1473101

End: 1474114

Strand: Direct

Name: cysP [H]

Synonym: GSU1346

Alternate gene names: 39996448

Gene position: 1473101-1474114 (Clockwise)

Preceding gene: 39996447

Following gene: 39996449

Centisome position: 38.62

GC content: 65.78

Gene sequence:

>1014_bases
ATGAAACCGATCAAGACCATCGCAGCCGCAGCCGCAGCATTCCTCGCCCTTGCCGCACCCCTGGGGGCCCGCGCCGAGGT
GAGCCTCCTCAACGTCTCCTACGACCCGACCCGGGAGCTGTACCAGGACTACAACGCCGCCTTTGCCGCCCACTGGAAGG
GGAAGGGCGGGGGGCCGGTCACGGTGAAACAGTCCCACGGCGGTTCGGGCAAACAGGCCCGGGCGGTCATCGACGGCCTG
GAGGCCGATGTGGTTACCCTGGCACTGGCCTATGACATCGACGAAATCGCGGGAAAGGCGAAGCTGATCCCTGCCAACTG
GCAGAAACGGCTGCCCCACAACAGCTCCCCCTACACCTCAACCATCGTGTTCCTGGTCCGCAAGGGGAACCCCAGGAACA
TCCGCGACTGGAACGACCTGGTCCGCCCCGACGTCAAGGTGATCACCCCCAATCCCAAAACCTCCGGCGGGGCCCGCTGG
AACTACCTGGCCGCCTGGGGCTACGCCCTGAAGCAGAAGGGGGGGAGCGAGGCAACGGCCCGCGACTTCGTGACCAGGCT
CTACAAGAACGTGCCGGTGCTCGATTCCGGTGCCCGGGGCGCCACCACCACCTTTGTCCAGCGCGGCCAGGGCGACGTGC
TCCTGGCGTGGGAGAACGAGGCCTTCCTGGCCGTGAACGAACTGGGCAAGGACAAATTCCAAATTGTGGTGCCGTCGGTC
AGCATCCTGGCCGAGCCGCCGGTGACGGTGGTGGACAAGGTGGTCGACCGGAAAGGGACCCGCGCGGTGGCCGAAGCGTA
CCTGAAGTACCTCTACACCCCGGCCGGCCAGGAGATCGCGGCCAAGCACTACTACCGTCCCATCGACAAGAACGTGGCGG
CCAAGTATGCCCCGGTCTTCCCGAAGGTGAAGCTCTTCACCATCGACGACACCTTCGGCGGCTGGCAGGCGGCCCAGAAG
AAGCACTTCGCCGACGGCGGGGTGTTCGACCGGATCTACGGCCCGGGCAAGTAA

Upstream 100 bases:

>100_bases
AAGAAGGACGGAGTGCTTTTTTACACGATCTGAACGGGGCGTTTTTTTATACTTTATTAGACTACTAAGTCGATAGACTA
AAAACCAAAAGGAGCCGACC

Downstream 100 bases:

>100_bases
CGTGATGCAGGGGCTGCGCGATGGCGTAAAATCCGCAGCCCCTGCATTGGGATTATGCAAAGGACCAACCACCATGGCGA
CAAGACCAATCACGCGAACC

Product: sulfate ABC transporter periplasmic sulfate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MKPIKTIAAAAAAFLALAAPLGARAEVSLLNVSYDPTRELYQDYNAAFAAHWKGKGGGPVTVKQSHGGSGKQARAVIDGL
EADVVTLALAYDIDEIAGKAKLIPANWQKRLPHNSSPYTSTIVFLVRKGNPRNIRDWNDLVRPDVKVITPNPKTSGGARW
NYLAAWGYALKQKGGSEATARDFVTRLYKNVPVLDSGARGATTTFVQRGQGDVLLAWENEAFLAVNELGKDKFQIVVPSV
SILAEPPVTVVDKVVDRKGTRAVAEAYLKYLYTPAGQEIAAKHYYRPIDKNVAAKYAPVFPKVKLFTIDDTFGGWQAAQK
KHFADGGVFDRIYGPGK

Sequences:

>Translated_337_residues
MKPIKTIAAAAAAFLALAAPLGARAEVSLLNVSYDPTRELYQDYNAAFAAHWKGKGGGPVTVKQSHGGSGKQARAVIDGL
EADVVTLALAYDIDEIAGKAKLIPANWQKRLPHNSSPYTSTIVFLVRKGNPRNIRDWNDLVRPDVKVITPNPKTSGGARW
NYLAAWGYALKQKGGSEATARDFVTRLYKNVPVLDSGARGATTTFVQRGQGDVLLAWENEAFLAVNELGKDKFQIVVPSV
SILAEPPVTVVDKVVDRKGTRAVAEAYLKYLYTPAGQEIAAKHYYRPIDKNVAAKYAPVFPKVKLFTIDDTFGGWQAAQK
KHFADGGVFDRIYGPGK
>Mature_337_residues
MKPIKTIAAAAAAFLALAAPLGARAEVSLLNVSYDPTRELYQDYNAAFAAHWKGKGGGPVTVKQSHGGSGKQARAVIDGL
EADVVTLALAYDIDEIAGKAKLIPANWQKRLPHNSSPYTSTIVFLVRKGNPRNIRDWNDLVRPDVKVITPNPKTSGGARW
NYLAAWGYALKQKGGSEATARDFVTRLYKNVPVLDSGARGATTTFVQRGQGDVLLAWENEAFLAVNELGKDKFQIVVPSV
SILAEPPVTVVDKVVDRKGTRAVAEAYLKYLYTPAGQEIAAKHYYRPIDKNVAAKYAPVFPKVKLFTIDDTFGGWQAAQK
KHFADGGVFDRIYGPGK

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=319, Percent_Identity=70.2194357366771, Blast_Score=465, Evalue=1e-132,
Organism=Escherichia coli, GI1788765, Length=312, Percent_Identity=50.6410256410256, Blast_Score=318, Evalue=4e-88,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36613; Mature: 36613

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIKTIAAAAAAFLALAAPLGARAEVSLLNVSYDPTRELYQDYNAAFAAHWKGKGGGPV
CCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHCCEEEEEECCCCCCEE
TVKQSHGGSGKQARAVIDGLEADVVTLALAYDIDEIAGKAKLIPANWQKRLPHNSSPYTS
EEECCCCCCCHHHHHHHHCCCHHHEEEEHHHCHHHHCCCEEEECCCHHHHCCCCCCCCCE
TIVFLVRKGNPRNIRDWNDLVRPDVKVITPNPKTSGGARWNYLAAWGYALKQKGGSEATA
EEEEEEECCCCCCCCCHHHHCCCCCEEECCCCCCCCCCCEEHHHHHHHHHHHCCCCCHHH
RDFVTRLYKNVPVLDSGARGATTTFVQRGQGDVLLAWENEAFLAVNELGKDKFQIVVPSV
HHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCEEEEECCCEEEEEECCCCCEEEEEECCH
SILAEPPVTVVDKVVDRKGTRAVAEAYLKYLYTPAGQEIAAKHYYRPIDKNVAAKYAPVF
HEECCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHCCCCC
PKVKLFTIDDTFGGWQAAQKKHFADGGVFDRIYGPGK
CCEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKPIKTIAAAAAAFLALAAPLGARAEVSLLNVSYDPTRELYQDYNAAFAAHWKGKGGGPV
CCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHCCEEEEEECCCCCCEE
TVKQSHGGSGKQARAVIDGLEADVVTLALAYDIDEIAGKAKLIPANWQKRLPHNSSPYTS
EEECCCCCCCHHHHHHHHCCCHHHEEEEHHHCHHHHCCCEEEECCCHHHHCCCCCCCCCE
TIVFLVRKGNPRNIRDWNDLVRPDVKVITPNPKTSGGARWNYLAAWGYALKQKGGSEATA
EEEEEEECCCCCCCCCHHHHCCCCCEEECCCCCCCCCCCEEHHHHHHHHHHHCCCCCHHH
RDFVTRLYKNVPVLDSGARGATTTFVQRGQGDVLLAWENEAFLAVNELGKDKFQIVVPSV
HHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCEEEEECCCEEEEEECCCCCEEEEEECCH
SILAEPPVTVVDKVVDRKGTRAVAEAYLKYLYTPAGQEIAAKHYYRPIDKNVAAKYAPVF
HEECCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHCCCCC
PKVKLFTIDDTFGGWQAAQKKHFADGGVFDRIYGPGK
CCEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]