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The map label for this gene is cysT [H]

Identifier: 39996449

GI number: 39996449

Start: 1474119

End: 1475021

Strand: Direct

Name: cysT [H]

Synonym: GSU1347

Alternate gene names: 39996449

Gene position: 1474119-1475021 (Clockwise)

Preceding gene: 39996448

Following gene: 39996450

Centisome position: 38.65

GC content: 67.33

Gene sequence:

>903_bases
ATGCAGGGGCTGCGCGATGGCGTAAAATCCGCAGCCCCTGCATTGGGATTATGCAAAGGACCAACCACCATGGCGACAAG
ACCAATCACGCGAACCACCGTCATTCCCGGCTTCGGCCCGGCCCTGGGCTACACGGTCTTCTACCTCTCCCTGGTCGTGC
TCCTGCCGCTCTCGGCCCTGGCGGTGAAAACGGCCAGCCTCACCTGGGGGGAATTCCTCGCCACCGTGGCAGCCCCCCGG
GTGCTGGCCTCCTACCGGGTCACCTTCGGCGCGGCCCTGGCCGCTGCCGGGGTCAACGCGGTCTTCGGTCTCTTGGTGGC
CTGGGTGCTCGTGCGCTACCGCGTGCCGGGCAAGCGGCTGATCGACGCCCTGGTCGATCTCCCCTTCGCGCTCCCCACGG
CCGTGGCCGGCATCACCCTGTCGTCGGTCTATGCCGCCAACGGCTGGATCGGCCGGTTCCTGGAGCCCCGGGGGATCGAG
GTCGCCTTCACCCCGCTGGGGATCTTCGTGGCCATGACCTTCATCGGTCTCCCCTTTGTGGTGCGGACGGTGCAGCCGGT
TCTGGAAGAGCTGGAAGAGGAGATCGAGGAGGCCGCCGCCTGCCTGGGCGCTACCCGCTGGCAGACCTTCCGCAGGGTCC
TGTTCCCGGAGCTGTTCCCGGCGGCGCTGACCGGCTTTGCCCTGGCCTTTGCCCGGGGCGTGGGCGAGTACGGCTCCATC
ATCTTCATCGCCGGCAACATGCCGATGGTGTCCGAGATCACCCCGCTCCTGATCATCACCAAGCTGGAGCAGTACGACTA
TGCCGGCGCCACCGCCATCGCCACGGTGATGCTCCTGTTCTCGTTCCTGATGCTCCTGGCCGTGAACCTGCTCCAGAAGT
GGAGCCGGCGCCACGCCGCCTGA

Upstream 100 bases:

>100_bases
ACCATCGACGACACCTTCGGCGGCTGGCAGGCGGCCCAGAAGAAGCACTTCGCCGACGGCGGGGTGTTCGACCGGATCTA
CGGCCCGGGCAAGTAACGTG

Downstream 100 bases:

>100_bases
CCCGGAGGACCCGACCATGCCATCATCCATCCGCACCCGTACCGAACCGGCCCCGGTCCGCTGGGCGCTCACCGCCGGCG
CGCTCCTGTTCCTCGGCCTC

Product: sulfate ABC transporter permease

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MQGLRDGVKSAAPALGLCKGPTTMATRPITRTTVIPGFGPALGYTVFYLSLVVLLPLSALAVKTASLTWGEFLATVAAPR
VLASYRVTFGAALAAAGVNAVFGLLVAWVLVRYRVPGKRLIDALVDLPFALPTAVAGITLSSVYAANGWIGRFLEPRGIE
VAFTPLGIFVAMTFIGLPFVVRTVQPVLEELEEEIEEAAACLGATRWQTFRRVLFPELFPAALTGFALAFARGVGEYGSI
IFIAGNMPMVSEITPLLIITKLEQYDYAGATAIATVMLLFSFLMLLAVNLLQKWSRRHAA

Sequences:

>Translated_300_residues
MQGLRDGVKSAAPALGLCKGPTTMATRPITRTTVIPGFGPALGYTVFYLSLVVLLPLSALAVKTASLTWGEFLATVAAPR
VLASYRVTFGAALAAAGVNAVFGLLVAWVLVRYRVPGKRLIDALVDLPFALPTAVAGITLSSVYAANGWIGRFLEPRGIE
VAFTPLGIFVAMTFIGLPFVVRTVQPVLEELEEEIEEAAACLGATRWQTFRRVLFPELFPAALTGFALAFARGVGEYGSI
IFIAGNMPMVSEITPLLIITKLEQYDYAGATAIATVMLLFSFLMLLAVNLLQKWSRRHAA
>Mature_300_residues
MQGLRDGVKSAAPALGLCKGPTTMATRPITRTTVIPGFGPALGYTVFYLSLVVLLPLSALAVKTASLTWGEFLATVAAPR
VLASYRVTFGAALAAAGVNAVFGLLVAWVLVRYRVPGKRLIDALVDLPFALPTAVAGITLSSVYAANGWIGRFLEPRGIE
VAFTPLGIFVAMTFIGLPFVVRTVQPVLEELEEEIEEAAACLGATRWQTFRRVLFPELFPAALTGFALAFARGVGEYGSI
IFIAGNMPMVSEITPLLIITKLEQYDYAGATAIATVMLLFSFLMLLAVNLLQKWSRRHAA

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=270, Percent_Identity=51.4814814814815, Blast_Score=251, Evalue=6e-68,
Organism=Escherichia coli, GI87082099, Length=243, Percent_Identity=32.9218106995885, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1786980, Length=200, Percent_Identity=33.5, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786254, Length=194, Percent_Identity=30.4123711340206, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787368, Length=202, Percent_Identity=29.2079207920792, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32125; Mature: 32125

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGLRDGVKSAAPALGLCKGPTTMATRPITRTTVIPGFGPALGYTVFYLSLVVLLPLSAL
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHH
AVKTASLTWGEFLATVAAPRVLASYRVTFGAALAAAGVNAVFGLLVAWVLVRYRVPGKRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
IDALVDLPFALPTAVAGITLSSVYAANGWIGRFLEPRGIEVAFTPLGIFVAMTFIGLPFV
HHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHH
VRTVQPVLEELEEEIEEAAACLGATRWQTFRRVLFPELFPAALTGFALAFARGVGEYGSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
IFIAGNMPMVSEITPLLIITKLEQYDYAGATAIATVMLLFSFLMLLAVNLLQKWSRRHAA
EEEECCCCCHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQGLRDGVKSAAPALGLCKGPTTMATRPITRTTVIPGFGPALGYTVFYLSLVVLLPLSAL
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHH
AVKTASLTWGEFLATVAAPRVLASYRVTFGAALAAAGVNAVFGLLVAWVLVRYRVPGKRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
IDALVDLPFALPTAVAGITLSSVYAANGWIGRFLEPRGIEVAFTPLGIFVAMTFIGLPFV
HHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHH
VRTVQPVLEELEEEIEEAAACLGATRWQTFRRVLFPELFPAALTGFALAFARGVGEYGSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
IFIAGNMPMVSEITPLLIITKLEQYDYAGATAIATVMLLFSFLMLLAVNLLQKWSRRHAA
EEEECCCCCHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1708375 [H]