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The map label for this gene is dctD [H]

Identifier: 39996418

GI number: 39996418

Start: 1442251

End: 1443225

Strand: Reverse

Name: dctD [H]

Synonym: GSU1316

Alternate gene names: 39996418

Gene position: 1443225-1442251 (Counterclockwise)

Preceding gene: 39996426

Following gene: 39996417

Centisome position: 37.84

GC content: 60.92

Gene sequence:

>975_bases
ATGGGACACGCCACCGAAATTCAAGTCCTCGTTGTGGACGAATCGGCCCCTTCCCGTGCGTTCATCGCATCGACCCTTGA
AAATGGCGGATATATCGTCACATCGGTCGCCTCCGGCGAGGAGGCCGTCCACGAGGTCAACGCCGCCCCCTTCGACATCC
TGGTGACCGACATCAATCTTCCCGGGATGAGCGGTCTCAATCTCCTCAAACTCCTGAAAGACTCATTCCCCGACCTGGAA
GTGGTGATCGTCACGAGCAACGCCTCCAGCTTCACCGCCATCAAGGCCCTTCGCCTGGGGGCCTACGATTTCATCATCAA
ACCCGTGGACGACCCCGCCATCCTGACCAACATCGTGGGCCGCGCCGTCGAGAAACAGTCCCTCACCCGTGAGAACCGGC
GGCTCATGGACGACATGAAACTGAAGAACCGGGAACTCCATGATGCACTCCACCTGATGAAATCTGCCAACCGCCTCTGC
ACGGCCATCTCGGCCTCTCTGGACGCGGGCGAGATCCTGAAGCGGCTGGTGGAAGGGGCCGTGGAGGAAGTAAAGGCGAA
AAAGGGATACCTTCTGCTCCTGGACCGTGATGGTAGTTCCTTCTCCATGAAAGTCAGCGTGGGTGTGAGCCGCACCCTCG
CCAAGGGATTCAGGCTCCGCCACGACCAAGGGATAGCCGGGCTCGTTGCCACCAACAACAAGCCCCTGCGCATCGACGAG
GAAGTGCCGCCCCCCCTCACCCACCGGATGCTGGAGGAAGACCCCAACGGCGAACTCTTCACAACTCCCGGCATCGTAAC
GGTGCCGCTCCAGTTCAAGGGGCGGGTGGCGGGCGTGGTAACCCTTTCGGGCAGGGCCGACGGCAGGGCTTTCACTGATG
CGGAAATCGAATTCCTCACCACCCTGGCGAACCACTCGGCCATCGCCCTTGACACGGCAGGTGCCTTCTACAAGCTCAGG
AAGGGCACCTCCTGA

Upstream 100 bases:

>100_bases
CGTAAACCAGTCGAATCGTGACCCCTTTCTGATTGATTTTTGTATCATCCTGAACTACCATTTGAAATTCAAGGTACAGT
ACGGGATGAGGAGGTGTACA

Downstream 100 bases:

>100_bases
CCCTTTGCCGCCTCCCCGCCCGTTGCATCACCGCTGCCATCCAGTACAATCCTTTATAACCCCGACGCCCCTGTGATATT
CCCTCTCCCCCGCCGTATCC

Product: response regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MGHATEIQVLVVDESAPSRAFIASTLENGGYIVTSVASGEEAVHEVNAAPFDILVTDINLPGMSGLNLLKLLKDSFPDLE
VVIVTSNASSFTAIKALRLGAYDFIIKPVDDPAILTNIVGRAVEKQSLTRENRRLMDDMKLKNRELHDALHLMKSANRLC
TAISASLDAGEILKRLVEGAVEEVKAKKGYLLLLDRDGSSFSMKVSVGVSRTLAKGFRLRHDQGIAGLVATNNKPLRIDE
EVPPPLTHRMLEEDPNGELFTTPGIVTVPLQFKGRVAGVVTLSGRADGRAFTDAEIEFLTTLANHSAIALDTAGAFYKLR
KGTS

Sequences:

>Translated_324_residues
MGHATEIQVLVVDESAPSRAFIASTLENGGYIVTSVASGEEAVHEVNAAPFDILVTDINLPGMSGLNLLKLLKDSFPDLE
VVIVTSNASSFTAIKALRLGAYDFIIKPVDDPAILTNIVGRAVEKQSLTRENRRLMDDMKLKNRELHDALHLMKSANRLC
TAISASLDAGEILKRLVEGAVEEVKAKKGYLLLLDRDGSSFSMKVSVGVSRTLAKGFRLRHDQGIAGLVATNNKPLRIDE
EVPPPLTHRMLEEDPNGELFTTPGIVTVPLQFKGRVAGVVTLSGRADGRAFTDAEIEFLTTLANHSAIALDTAGAFYKLR
KGTS
>Mature_323_residues
GHATEIQVLVVDESAPSRAFIASTLENGGYIVTSVASGEEAVHEVNAAPFDILVTDINLPGMSGLNLLKLLKDSFPDLEV
VIVTSNASSFTAIKALRLGAYDFIIKPVDDPAILTNIVGRAVEKQSLTRENRRLMDDMKLKNRELHDALHLMKSANRLCT
AISASLDAGEILKRLVEGAVEEVKAKKGYLLLLDRDGSSFSMKVSVGVSRTLAKGFRLRHDQGIAGLVATNNKPLRIDEE
VPPPLTHRMLEEDPNGELFTTPGIVTVPLQFKGRVAGVVTLSGRADGRAFTDAEIEFLTTLANHSAIALDTAGAFYKLRK
GTS

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=126, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790437, Length=104, Percent_Identity=35.5769230769231, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788550, Length=128, Percent_Identity=32.03125, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1789809, Length=116, Percent_Identity=31.8965517241379, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 34959; Mature: 34827

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHATEIQVLVVDESAPSRAFIASTLENGGYIVTSVASGEEAVHEVNAAPFDILVTDINL
CCCCEEEEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCEEEEEEEECC
PGMSGLNLLKLLKDSFPDLEVVIVTSNASSFTAIKALRLGAYDFIIKPVDDPAILTNIVG
CCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHH
RAVEKQSLTRENRRLMDDMKLKNRELHDALHLMKSANRLCTAISASLDAGEILKRLVEGA
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VEEVKAKKGYLLLLDRDGSSFSMKVSVGVSRTLAKGFRLRHDQGIAGLVATNNKPLRIDE
HHHHHCCCCEEEEEECCCCCEEEEEECCHHHHHHHHHEEECCCCEEEEEEECCCCEEECC
EVPPPLTHRMLEEDPNGELFTTPGIVTVPLQFKGRVAGVVTLSGRADGRAFTDAEIEFLT
CCCCHHHHHHHHCCCCCCEEECCCEEEEEEEECCEEEEEEEECCCCCCCEECCCHHHEEE
TLANHSAIALDTAGAFYKLRKGTS
HHHCCCEEEEECCCCEEEECCCCC
>Mature Secondary Structure 
GHATEIQVLVVDESAPSRAFIASTLENGGYIVTSVASGEEAVHEVNAAPFDILVTDINL
CCCEEEEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCEEEEEEEECC
PGMSGLNLLKLLKDSFPDLEVVIVTSNASSFTAIKALRLGAYDFIIKPVDDPAILTNIVG
CCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHH
RAVEKQSLTRENRRLMDDMKLKNRELHDALHLMKSANRLCTAISASLDAGEILKRLVEGA
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VEEVKAKKGYLLLLDRDGSSFSMKVSVGVSRTLAKGFRLRHDQGIAGLVATNNKPLRIDE
HHHHHCCCCEEEEEECCCCCEEEEEECCHHHHHHHHHEEECCCCEEEEEEECCCCEEECC
EVPPPLTHRMLEEDPNGELFTTPGIVTVPLQFKGRVAGVVTLSGRADGRAFTDAEIEFLT
CCCCHHHHHHHHCCCCCCEEECCCEEEEEEEECCEEEEEEEECCCCCCCEECCCHHHEEE
TLANHSAIALDTAGAFYKLRKGTS
HHHCCCEEEEECCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]