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The map label for this gene is merA-1 [H]

Identifier: 39996417

GI number: 39996417

Start: 1440620

End: 1442137

Strand: Reverse

Name: merA-1 [H]

Synonym: GSU1315

Alternate gene names: 39996417

Gene position: 1442137-1440620 (Counterclockwise)

Preceding gene: 39996418

Following gene: 39996416

Centisome position: 37.81

GC content: 70.82

Gene sequence:

>1518_bases
GTGTCAGACAATTTCGCTTCCCCGCACGACCGGGACCTTGACGAGCGGGTGCGCCCGCCCGGCTGGATCAACCCGTCTCC
GGCTCCGCGCTATGACCTGGTGGTCGTTGGTGCCGGCACGGCCGGACTCGTCTGCGCGGCAGGCGCAGCAGGGCTCGGTG
CGCGTGTCGCGCTGGTGGAGCGCCACCGGCTGGGGGGCGACTGTCTCAACTACGGCTGCGTCCCATCCAAGGCCCTCATC
CGCGCCGCGCGGGCCGCTCACGATGCCGGGAACGGCGCCCCCTTCGGCGTGACGGGGTGCCACGGGACCGGCGTCGACGG
TGCGGCCGTCATGGAGCGGATGCGCCGCCTGCGGGCGGAAATCGGCCGTCACGACGCGGCAGTGCGCTTTCGTGACCTGG
GGGTCCACGTCTTCTTCGGCCAGGGTAGCTTCATCAGCCGGAACGCCCTGGAGGTGGACGGACGGCGCCTGAATTTCGTT
CATGCCGCCGTCTGCACCGGTGCCCGGGCCGCAGCCCCTCCGGTCCCGGGGCTGGCGGAAGCGGGGTACCTCACCAACGA
GACGATTTTTTCCCTCGCAACGCTTCCGGCGCGACTGGCCGTCATCGGCGGCGGCCCCATCGGCTGCGAGCTGGCCCAGG
CCGCGGCACGGCTCGGCAGCAGCGTGACGGTGATCGAGGCCGCCCCGGAGATCCTGCCGCGGGAGGACACCGACGCCGCG
GCCCTGGTGCGGCACGCCCTGGAGCGGGACAGGGTATCGTTCCTGACGGCGGCAGCTGTCGTCGGGGTGGAACGGCGGAG
CGGAGCCCGGACGCTCATCGTCCGGCAGGGGGATCAATCCCATGAGGTGACGGCTGACGAGATCCTGGTGGGGGCCGGGC
GAACGCCGAACATCGAAGGGCTGGGACTGGAGCGGGCGGGCATTGTCGCCGATCCGCTCCGAGGGGTCAGGGTCAACGAC
CGGCTCCGGACCGACAATCCGCGAGTCTACGCCGCCGGCGACATCTGCTCCCCCTACCGATTCACCCACGCCGCAGACGC
CATGGCGCGCATCGTCGTCGCCAACGCCCTCTTCGGCGCCCGGCAGCGGTTCTCTACCCAGATCATTCCCTGGTGCACCT
ACACCGACCCGGAGGTTGCCCACGTGGGCCTCTACGAACGGGAGGCCGGGGAGCGCGGTCTTGCCGTGGACACCCTGACC
GTCCCCCTGACCGAGGTCGACCGGGCTCTGCTTGACGGGGAAGACGAGGGTTTCGCCCGGGTGCACCTGAAGCGGGGCAC
CGACCGGATCGTCGGCGCCACCATCGTGGCCCGCCACGCCGGCGAGATGCTGAACGAGCTGACCCTGGCCATGTCCGCCG
GATTGGGCCTTTCCGCCATCGGCCGCAGCATCCACCCCTACCCCACTCAGGCCGAAGCGATCAAGAAGCTGGCCGATGCC
TGGAACCGGACCCGCCTCACCCCCGGGGTGAAGCGGCTGATGGGGATCATGCTGACCCTGCGCCGGCTGTGGCGCTAG

Upstream 100 bases:

>100_bases
CCCCGCCCGTTGCATCACCGCTGCCATCCAGTACAATCCTTTATAACCCCGACGCCCCTGTGATATTCCCTCTCCCCCGC
CGTATCCGCGGAGGAACGCC

Downstream 100 bases:

>100_bases
CCATGAACTACGTGCGAATCATGGAAAATATGAATATATTCCTATAGCCGATTGCCTCCGCTCCCGGCCTGGTATATAAC
CATCGTTCATGAACGCACAA

Product: mercuric reductase

Products: NA

Alternate protein names: Hg(II) reductase [H]

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MSDNFASPHDRDLDERVRPPGWINPSPAPRYDLVVVGAGTAGLVCAAGAAGLGARVALVERHRLGGDCLNYGCVPSKALI
RAARAAHDAGNGAPFGVTGCHGTGVDGAAVMERMRRLRAEIGRHDAAVRFRDLGVHVFFGQGSFISRNALEVDGRRLNFV
HAAVCTGARAAAPPVPGLAEAGYLTNETIFSLATLPARLAVIGGGPIGCELAQAAARLGSSVTVIEAAPEILPREDTDAA
ALVRHALERDRVSFLTAAAVVGVERRSGARTLIVRQGDQSHEVTADEILVGAGRTPNIEGLGLERAGIVADPLRGVRVND
RLRTDNPRVYAAGDICSPYRFTHAADAMARIVVANALFGARQRFSTQIIPWCTYTDPEVAHVGLYEREAGERGLAVDTLT
VPLTEVDRALLDGEDEGFARVHLKRGTDRIVGATIVARHAGEMLNELTLAMSAGLGLSAIGRSIHPYPTQAEAIKKLADA
WNRTRLTPGVKRLMGIMLTLRRLWR

Sequences:

>Translated_505_residues
MSDNFASPHDRDLDERVRPPGWINPSPAPRYDLVVVGAGTAGLVCAAGAAGLGARVALVERHRLGGDCLNYGCVPSKALI
RAARAAHDAGNGAPFGVTGCHGTGVDGAAVMERMRRLRAEIGRHDAAVRFRDLGVHVFFGQGSFISRNALEVDGRRLNFV
HAAVCTGARAAAPPVPGLAEAGYLTNETIFSLATLPARLAVIGGGPIGCELAQAAARLGSSVTVIEAAPEILPREDTDAA
ALVRHALERDRVSFLTAAAVVGVERRSGARTLIVRQGDQSHEVTADEILVGAGRTPNIEGLGLERAGIVADPLRGVRVND
RLRTDNPRVYAAGDICSPYRFTHAADAMARIVVANALFGARQRFSTQIIPWCTYTDPEVAHVGLYEREAGERGLAVDTLT
VPLTEVDRALLDGEDEGFARVHLKRGTDRIVGATIVARHAGEMLNELTLAMSAGLGLSAIGRSIHPYPTQAEAIKKLADA
WNRTRLTPGVKRLMGIMLTLRRLWR
>Mature_504_residues
SDNFASPHDRDLDERVRPPGWINPSPAPRYDLVVVGAGTAGLVCAAGAAGLGARVALVERHRLGGDCLNYGCVPSKALIR
AARAAHDAGNGAPFGVTGCHGTGVDGAAVMERMRRLRAEIGRHDAAVRFRDLGVHVFFGQGSFISRNALEVDGRRLNFVH
AAVCTGARAAAPPVPGLAEAGYLTNETIFSLATLPARLAVIGGGPIGCELAQAAARLGSSVTVIEAAPEILPREDTDAAA
LVRHALERDRVSFLTAAAVVGVERRSGARTLIVRQGDQSHEVTADEILVGAGRTPNIEGLGLERAGIVADPLRGVRVNDR
LRTDNPRVYAAGDICSPYRFTHAADAMARIVVANALFGARQRFSTQIIPWCTYTDPEVAHVGLYEREAGERGLAVDTLTV
PLTEVDRALLDGEDEGFARVHLKRGTDRIVGATIVARHAGEMLNELTLAMSAGLGLSAIGRSIHPYPTQAEAIKKLADAW
NRTRLTPGVKRLMGIMLTLRRLWR

Specific function: Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0) [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=459, Percent_Identity=30.2832244008715, Blast_Score=186, Evalue=6e-47,
Organism=Homo sapiens, GI50301238, Length=464, Percent_Identity=27.5862068965517, Blast_Score=138, Evalue=9e-33,
Organism=Homo sapiens, GI22035672, Length=499, Percent_Identity=26.4529058116232, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI291045266, Length=484, Percent_Identity=26.4462809917355, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI33519430, Length=500, Percent_Identity=24.8, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI33519428, Length=500, Percent_Identity=24.8, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI33519426, Length=500, Percent_Identity=24.8, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI148277065, Length=500, Percent_Identity=24.8, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI148277071, Length=500, Percent_Identity=24.8, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI291045268, Length=475, Percent_Identity=24.2105263157895, Blast_Score=83, Evalue=6e-16,
Organism=Escherichia coli, GI1786307, Length=455, Percent_Identity=30.7692307692308, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI87081717, Length=466, Percent_Identity=29.8283261802575, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI87082354, Length=459, Percent_Identity=24.400871459695, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1789915, Length=438, Percent_Identity=26.027397260274, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI32565766, Length=473, Percent_Identity=29.8097251585624, Blast_Score=186, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17557007, Length=475, Percent_Identity=25.6842105263158, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71983419, Length=431, Percent_Identity=23.6658932714617, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71983429, Length=431, Percent_Identity=23.6658932714617, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71982272, Length=488, Percent_Identity=23.9754098360656, Blast_Score=88, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6321091, Length=473, Percent_Identity=30.2325581395349, Blast_Score=177, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6325166, Length=467, Percent_Identity=24.6252676659529, Blast_Score=123, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6325240, Length=482, Percent_Identity=24.0663900414938, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21358499, Length=457, Percent_Identity=31.2910284463895, Blast_Score=194, Evalue=9e-50,
Organism=Drosophila melanogaster, GI24640551, Length=481, Percent_Identity=26.8191268191268, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24640549, Length=482, Percent_Identity=26.9709543568465, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24640553, Length=492, Percent_Identity=27.0325203252033, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI17737741, Length=488, Percent_Identity=26.844262295082, Blast_Score=122, Evalue=5e-28,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000815
- InterPro:   IPR021179
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00403 HMA; PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.16.1.1 [H]

Molecular weight: Translated: 53715; Mature: 53584

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNFASPHDRDLDERVRPPGWINPSPAPRYDLVVVGAGTAGLVCAAGAAGLGARVALVE
CCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEEEEECCCHHHHHHCCCCCCCCEEEEHH
RHRLGGDCLNYGCVPSKALIRAARAAHDAGNGAPFGVTGCHGTGVDGAAVMERMRRLRAE
HHHCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
IGRHDAAVRFRDLGVHVFFGQGSFISRNALEVDGRRLNFVHAAVCTGARAAAPPVPGLAE
HCCCHHEEEEEECCEEEEECCCCEEECCCEEECCCEEEEEHHHHHCCCCCCCCCCCCCHH
AGYLTNETIFSLATLPARLAVIGGGPIGCELAQAAARLGSSVTVIEAAPEILPREDTDAA
CCCCCCHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCHHHCCCCCCCHH
ALVRHALERDRVSFLTAAAVVGVERRSGARTLIVRQGDQSHEVTADEILVGAGRTPNIEG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHEEEECCCCCCCCC
LGLERAGIVADPLRGVRVNDRLRTDNPRVYAAGDICSPYRFTHAADAMARIVVANALFGA
CCCCCCCCEECCCCCCEECCCEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
RQRFSTQIIPWCTYTDPEVAHVGLYEREAGERGLAVDTLTVPLTEVDRALLDGEDEGFAR
HHHHCCEEEEEEECCCCCCEEECCHHHCCCCCCCEEEEEECCHHHHHHHHHCCCCCCEEE
VHLKRGTDRIVGATIVARHAGEMLNELTLAMSAGLGLSAIGRSIHPYPTQAEAIKKLADA
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHH
WNRTRLTPGVKRLMGIMLTLRRLWR
HHCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDNFASPHDRDLDERVRPPGWINPSPAPRYDLVVVGAGTAGLVCAAGAAGLGARVALVE
CCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEEEEECCCHHHHHHCCCCCCCCEEEEHH
RHRLGGDCLNYGCVPSKALIRAARAAHDAGNGAPFGVTGCHGTGVDGAAVMERMRRLRAE
HHHCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
IGRHDAAVRFRDLGVHVFFGQGSFISRNALEVDGRRLNFVHAAVCTGARAAAPPVPGLAE
HCCCHHEEEEEECCEEEEECCCCEEECCCEEECCCEEEEEHHHHHCCCCCCCCCCCCCHH
AGYLTNETIFSLATLPARLAVIGGGPIGCELAQAAARLGSSVTVIEAAPEILPREDTDAA
CCCCCCHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCHHHCCCCCCCHH
ALVRHALERDRVSFLTAAAVVGVERRSGARTLIVRQGDQSHEVTADEILVGAGRTPNIEG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHEEEECCCCCCCCC
LGLERAGIVADPLRGVRVNDRLRTDNPRVYAAGDICSPYRFTHAADAMARIVVANALFGA
CCCCCCCCEECCCCCCEECCCEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
RQRFSTQIIPWCTYTDPEVAHVGLYEREAGERGLAVDTLTVPLTEVDRALLDGEDEGFAR
HHHHCCEEEEEEECCCCCCEEECCHHHCCCCCCCEEEEEECCHHHHHHHHHCCCCCCEEE
VHLKRGTDRIVGATIVARHAGEMLNELTLAMSAGLGLSAIGRSIHPYPTQAEAIKKLADA
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHH
WNRTRLTPGVKRLMGIMLTLRRLWR
HHCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3037534 [H]