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The map label for this gene is pyrB

Identifier: 39996373

GI number: 39996373

Start: 1385636

End: 1386571

Strand: Direct

Name: pyrB

Synonym: GSU1271

Alternate gene names: 39996373

Gene position: 1385636-1386571 (Clockwise)

Preceding gene: 39996372

Following gene: 39996374

Centisome position: 36.33

GC content: 63.46

Gene sequence:

>936_bases
ATGGGGTTCAAGCATAAGGATATCATCGGGTTGCAGGATCTGACCCGGGAGGAAATTCAGCTCCTCCTCGACACGGCCGA
CAACATGAAGGAGATCAACTCCCGCGACATCAAGAAGGTGCCGACCCTGCGCGGCAAGACCGTGGTGAACGTCTTCTACG
AGGCATCCACCCGGACCCGGACCTCCTTCGAGATTGCGGCCAAGCGCCTTTCCGCCGATACGATCAACATCTCGGCCTCC
ACCAGTTCCGTGACCAAGGGGGAGACCCTTTCCGATACGGCCCGAAACATCCTGGCCATGAAGCCCGACATCATCGTCAT
GCGCCACGCCGCTTCGGGCGCTCACCACTATCTGGCCCAGCGGGTCTCCTGCTCGGTGATCAATGCCGGCGACGGCGCCC
ATGAGCACCCCTCCCAGGGGCTTCTGGACATGCTGACCATGCGCCAGAAGTTCGGAACGATCGAAGGGCTCACGGTGGCC
ATCGTGGGTGACATCACCCACAGCCGCGTGGCCCGCTCCGACATCTTCGGCCTCACTAAAATGGGGGCGCATGTCCGTCT
CGCCGGCCCACCGACCATGATGCCCCCGGGCATCGAGCGCCTCGGCAACGTGACCGTCTGCCGCGACATGCGTGAGGCCA
TCGAGGGGGTCGATGTGGTGATGATGCTCCGCATCCAGCTTGAGCGCCAGGGCAAGACCCTGTTGCCGACCCTGCGTGAG
TATGCCCGCTACTACGGCCTCAATCCCCAGAACCTGAAGTTGGCCAAGCCCGGCGCCATGGTCATGCACCCGGGCCCCAT
CAACCGGGGGGTGGAGCTTTCCTCCTACGTGGCGGACAGCGACCAGTCCGCGATTCTCACCCAGGTCGAGAACGGCGTGG
CCGTGAGGATGGCGATGTTGTACCACGTGAGCGGCGGTGAGCTGGCCACCGAGTGA

Upstream 100 bases:

>100_bases
CCAACCGGCCCACTGATGTTGTGCTGGAGAAGTGAACAGGGACGAGACGCAGCGAACAACTACCATTACCTGCCCGCGAC
GCGGGCAAACGGGGGGAACG

Downstream 100 bases:

>100_bases
GCCGGCTGTTCGGGATACTTTAACCAACCGAGATTCGTGAGGGGTGATGATATGAACCTGCTGATAAAAGGTGGGAGGGT
GATTGACCCGTCCCAGGGAA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MGFKHKDIIGLQDLTREEIQLLLDTADNMKEINSRDIKKVPTLRGKTVVNVFYEASTRTRTSFEIAAKRLSADTINISAS
TSSVTKGETLSDTARNILAMKPDIIVMRHAASGAHHYLAQRVSCSVINAGDGAHEHPSQGLLDMLTMRQKFGTIEGLTVA
IVGDITHSRVARSDIFGLTKMGAHVRLAGPPTMMPPGIERLGNVTVCRDMREAIEGVDVVMMLRIQLERQGKTLLPTLRE
YARYYGLNPQNLKLAKPGAMVMHPGPINRGVELSSYVADSDQSAILTQVENGVAVRMAMLYHVSGGELATE

Sequences:

>Translated_311_residues
MGFKHKDIIGLQDLTREEIQLLLDTADNMKEINSRDIKKVPTLRGKTVVNVFYEASTRTRTSFEIAAKRLSADTINISAS
TSSVTKGETLSDTARNILAMKPDIIVMRHAASGAHHYLAQRVSCSVINAGDGAHEHPSQGLLDMLTMRQKFGTIEGLTVA
IVGDITHSRVARSDIFGLTKMGAHVRLAGPPTMMPPGIERLGNVTVCRDMREAIEGVDVVMMLRIQLERQGKTLLPTLRE
YARYYGLNPQNLKLAKPGAMVMHPGPINRGVELSSYVADSDQSAILTQVENGVAVRMAMLYHVSGGELATE
>Mature_310_residues
GFKHKDIIGLQDLTREEIQLLLDTADNMKEINSRDIKKVPTLRGKTVVNVFYEASTRTRTSFEIAAKRLSADTINISAST
SSVTKGETLSDTARNILAMKPDIIVMRHAASGAHHYLAQRVSCSVINAGDGAHEHPSQGLLDMLTMRQKFGTIEGLTVAI
VGDITHSRVARSDIFGLTKMGAHVRLAGPPTMMPPGIERLGNVTVCRDMREAIEGVDVVMMLRIQLERQGKTLLPTLREY
ARYYGLNPQNLKLAKPGAMVMHPGPINRGVELSSYVADSDQSAILTQVENGVAVRMAMLYHVSGGELATE

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=309, Percent_Identity=35.9223300970874, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI38788445, Length=311, Percent_Identity=24.7588424437299, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI2367364, Length=310, Percent_Identity=34.8387096774194, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1790703, Length=325, Percent_Identity=26.7692307692308, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1786469, Length=332, Percent_Identity=26.2048192771084, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI193204318, Length=322, Percent_Identity=34.472049689441, Blast_Score=138, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6322331, Length=316, Percent_Identity=32.2784810126582, Blast_Score=153, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24642586, Length=314, Percent_Identity=33.1210191082803, Blast_Score=161, Evalue=5e-40,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_GEOSL (Q74DP5)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_952324.1
- ProteinModelPortal:   Q74DP5
- SMR:   Q74DP5
- GeneID:   2685916
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU1271
- NMPDR:   fig|243231.1.peg.1262
- TIGR:   GSU1271
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   GSUL243231:GSU_1271-MONOMER
- BRENDA:   2.1.3.2
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 33992; Mature: 33861

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFKHKDIIGLQDLTREEIQLLLDTADNMKEINSRDIKKVPTLRGKTVVNVFYEASTRTR
CCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHCCCCHHHCCCCCCCEEEEEEEECCCCCC
TSFEIAAKRLSADTINISASTSSVTKGETLSDTARNILAMKPDIIVMRHAASGAHHYLAQ
HHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHEEECCCEEEEECCCCCHHHHHHH
RVSCSVINAGDGAHEHPSQGLLDMLTMRQKFGTIEGLTVAIVGDITHSRVARSDIFGLTK
HHHEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHH
MGAHVRLAGPPTMMPPGIERLGNVTVCRDMREAIEGVDVVMMLRIQLERQGKTLLPTLRE
CCCEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHEEHHHHHHHHCCCCHHHHHHHH
YARYYGLNPQNLKLAKPGAMVMHPGPINRGVELSSYVADSDQSAILTQVENGVAVRMAML
HHHHHCCCCCCEEEECCCEEEECCCCCCCCCCHHHHHCCCCCHHHHHHHCCCCEEEEEEE
YHVSGGELATE
EEECCCCCCCC
>Mature Secondary Structure 
GFKHKDIIGLQDLTREEIQLLLDTADNMKEINSRDIKKVPTLRGKTVVNVFYEASTRTR
CCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHCCCCHHHCCCCCCCEEEEEEEECCCCCC
TSFEIAAKRLSADTINISASTSSVTKGETLSDTARNILAMKPDIIVMRHAASGAHHYLAQ
HHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHEEECCCEEEEECCCCCHHHHHHH
RVSCSVINAGDGAHEHPSQGLLDMLTMRQKFGTIEGLTVAIVGDITHSRVARSDIFGLTK
HHHEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHH
MGAHVRLAGPPTMMPPGIERLGNVTVCRDMREAIEGVDVVMMLRIQLERQGKTLLPTLRE
CCCEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHEEHHHHHHHHCCCCHHHHHHHH
YARYYGLNPQNLKLAKPGAMVMHPGPINRGVELSSYVADSDQSAILTQVENGVAVRMAML
HHHHHCCCCCCEEEECCCEEEECCCCCCCCCCHHHHHCCCCCHHHHHHHCCCCEEEEEEE
YHVSGGELATE
EEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA