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The map label for this gene is hydN [H]

Identifier: 39996340

GI number: 39996340

Start: 1340554

End: 1341003

Strand: Reverse

Name: hydN [H]

Synonym: GSU1238

Alternate gene names: 39996340

Gene position: 1341003-1340554 (Counterclockwise)

Preceding gene: 39996341

Following gene: 39996339

Centisome position: 35.16

GC content: 62.67

Gene sequence:

>450_bases
ATGAAACGTATCTACATGATTGAAGACGCCTGCATCGGCTGTCACCTGTGTGAGGTCGGCTGCATCACCGAACATTCGGC
ATCCAAGGATCCCGTGCGAGCCTACCTGCACGAGCCCGAACGCCCCATTGCCCGCTGCACCGTGGAGGAGATCGACGGCG
GCATCGTCTCCTTCTCCACCACCTGCCGCCATTGCGACGAGCCCGACTGTCTGCGGGCCTGCATCTCCGGTGCCATCCAG
AAGAACGACCAGGGCGTGGTCCGGATCGACACCGAGCAGTGCGTGGGCTGCTGGTCCTGCGTCATGGCCTGCCCCTACGG
TGCCATCCAGCGGAACCTGAAGAAAAAGAAGGCGAACAAGTGCGACCTCTGCCCGGACCGCATGTCTCCGGCCTGCGTCG
ACGCCTGCCCCAATCGGGCGCTTGTCTACAAGGAGGGGAGCCAGAAATGA

Upstream 100 bases:

>100_bases
CTGGAATCCCTCCGTGGCAACCGCTACAAGCTGCGGGCCGTGGGCCTGGGCGAAAAAGACATGAACATTCTCGGCGTCAT
GCCGGCTGGAGAATAGCCCC

Downstream 100 bases:

>100_bases
ACTACGTGATCATCGGCAACTCGGTGGCTGCCGTAGGAGCCATCCGGGGCATCAGGAGCATCGACCAACAGGGGAATATA
ACCGTCATCTCCCGCGAGCG

Product: iron-sulfur cluster-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MKRIYMIEDACIGCHLCEVGCITEHSASKDPVRAYLHEPERPIARCTVEEIDGGIVSFSTTCRHCDEPDCLRACISGAIQ
KNDQGVVRIDTEQCVGCWSCVMACPYGAIQRNLKKKKANKCDLCPDRMSPACVDACPNRALVYKEGSQK

Sequences:

>Translated_149_residues
MKRIYMIEDACIGCHLCEVGCITEHSASKDPVRAYLHEPERPIARCTVEEIDGGIVSFSTTCRHCDEPDCLRACISGAIQ
KNDQGVVRIDTEQCVGCWSCVMACPYGAIQRNLKKKKANKCDLCPDRMSPACVDACPNRALVYKEGSQK
>Mature_149_residues
MKRIYMIEDACIGCHLCEVGCITEHSASKDPVRAYLHEPERPIARCTVEEIDGGIVSFSTTCRHCDEPDCLRACISGAIQ
KNDQGVVRIDTEQCVGCWSCVMACPYGAIQRNLKKKKANKCDLCPDRMSPACVDACPNRALVYKEGSQK

Specific function: Electron transport from formate to hydrogen [H]

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789067, Length=156, Percent_Identity=39.7435897435897, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1787122, Length=150, Percent_Identity=34.6666666666667, Blast_Score=87, Evalue=6e-19,
Organism=Escherichia coli, GI1787872, Length=150, Percent_Identity=34.6666666666667, Blast_Score=87, Evalue=6e-19,
Organism=Escherichia coli, GI1788811, Length=150, Percent_Identity=36.6666666666667, Blast_Score=86, Evalue=8e-19,
Organism=Escherichia coli, GI226510944, Length=149, Percent_Identity=33.5570469798658, Blast_Score=83, Evalue=8e-18,
Organism=Escherichia coli, GI87082179, Length=147, Percent_Identity=36.734693877551, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI2367245, Length=142, Percent_Identity=36.6197183098592, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI87082180, Length=144, Percent_Identity=33.3333333333333, Blast_Score=76, Evalue=8e-16,
Organism=Escherichia coli, GI1787749, Length=155, Percent_Identity=34.1935483870968, Blast_Score=76, Evalue=9e-16,
Organism=Escherichia coli, GI1790326, Length=149, Percent_Identity=32.2147651006711, Blast_Score=71, Evalue=3e-14,
Organism=Escherichia coli, GI2367345, Length=147, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=6e-13,
Organism=Escherichia coli, GI1787963, Length=136, Percent_Identity=30.8823529411765, Blast_Score=66, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 16474; Mature: 16474

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

11.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
14.1 %Cys+Met (Translated Protein)
11.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
14.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIYMIEDACIGCHLCEVGCITEHSASKDPVRAYLHEPERPIARCTVEEIDGGIVSFST
CCEEEEECCCCHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHCCCEEEECH
TCRHCDEPDCLRACISGAIQKNDQGVVRIDTEQCVGCWSCVMACPYGAIQRNLKKKKANK
HHCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
CDLCPDRMSPACVDACPNRALVYKEGSQK
CCCCCCCCCCHHHHHCCCCEEEECCCCCC
>Mature Secondary Structure
MKRIYMIEDACIGCHLCEVGCITEHSASKDPVRAYLHEPERPIARCTVEEIDGGIVSFST
CCEEEEECCCCHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHCCCEEEECH
TCRHCDEPDCLRACISGAIQKNDQGVVRIDTEQCVGCWSCVMACPYGAIQRNLKKKKANK
HHCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
CDLCPDRMSPACVDACPNRALVYKEGSQK
CCCCCCCCCCHHHHHCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]