Click here to switch to the map view.

The map label for this gene is nasD [H]

Identifier: 39996339

GI number: 39996339

Start: 1339247

End: 1340557

Strand: Reverse

Name: nasD [H]

Synonym: GSU1237

Alternate gene names: 39996339

Gene position: 1340557-1339247 (Counterclockwise)

Preceding gene: 39996340

Following gene: 39996338

Centisome position: 35.15

GC content: 62.78

Gene sequence:

>1311_bases
ATGAACTACGTGATCATCGGCAACTCGGTGGCTGCCGTAGGAGCCATCCGGGGCATCAGGAGCATCGACCAACAGGGGAA
TATAACCGTCATCTCCCGCGAGCGCCACAACGCCTATGGCCGCCCACTCATCTCCTACCTCCTGGGAGGGCTTGTCTCGG
AAAAGCGGATGGCGTACCTCCCCGAGGACTTTTACGAAAAGAACCGCGTCAACCTGCTGCTCAACTCGGAAGTGACGGGT
ATCGACACCGCCGCCCGCCAAGTGCGGATCGCCGGCGGGGACACCATCGGCTATGACCGGCTCCTGGTCGCCACCGGCGG
CGACCCCTTCATCCCCCCCATCGAGGGAATGGCCGACAAGGACCGGATCTTCACCTTCACCACCTGGGACGACGCGGCCA
AGCTCAAGGGAATAGCGTCCGACATCGGCCGTGTGGTGGTTATCGGAGGCGGCCTCATCGGCCTCAAGGCCGCCGAAGGG
CTCCACCTGATCGGCAAGCAGATTACCATCGTGGAACTGGCCGACCGGATTCTCTCCGCCGCCTTCGACCGCCCTGCCGG
GCGCGTGGTAGCCAAGAAGATGAAGGCCAACGGCATCGACGTCATCACTGAAGATACGGTCGTCCGGATCGAGGGGGAAG
GGGCCGAGATTACCGGCGTAACGCTCCGCTCCGGCGATTTTATCCCCTGCGATACGATTATCGTGGCCATCGGGGTGCGC
CCCGCCTGCGGCTTCCTCAAGGGAAGCGGGGTTGAGGTGAACCGCGGCATCGTGGTCGACGACCGGATGGAGACTTCGGT
CGAAGGCATTTATGCCGCGGGCGACGTGGCTGAAGCCAAGGACTTCTTCTCCGGCGTCAAGAACCCCATGCCCATCTGGC
CCGATGCGTACATCCAGGGCGACATCGCCGGGGTGAGCATGGCCGGCGGCACCAAGGCCTACGAAGGGGGCCTGGCCATG
AACTCCATCGAGCTCTTCAAGGTATCGACCATTTCCATGGGGATCACCAATCCGGCGGAACCCAAAGAGTACGAAATCCT
CACCTACCAGGACCTGGAAAACTACCAGTACCGCAAGATCGTCATCAAGGACGGGCTGCTGGCGGGTGCGGTCCTCGTGG
GTGCAGTGGACCGGGCCGGAATTTTCTCGGGACTCATCCGGGAGAAGATCGCCGTTGTCCCCTTCAAGGACCAGTTGCTC
GCGCCTGACTTCGGCTTTGCCAACCTGCCGAAGGATATCCGGACCGCCCTTTTCGCACCGGCCGGCAAGGTGACGGAAAC
CGGCAGACCGGCCGTGGCGGTGGGGCACTGA

Upstream 100 bases:

>100_bases
AGAAGGCGAACAAGTGCGACCTCTGCCCGGACCGCATGTCTCCGGCCTGCGTCGACGCCTGCCCCAATCGGGCGCTTGTC
TACAAGGAGGGGAGCCAGAA

Downstream 100 bases:

>100_bases
AGGACCGGCAGCTCACACGATTCGACGACCAGACCGAGGTTCGAACAATGATACAGACAACGCGGAAACCGACGCATAAC
TTCCAGAAGGACATCTCCAA

Product: pyridine nucleotide-disulphide oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MNYVIIGNSVAAVGAIRGIRSIDQQGNITVISRERHNAYGRPLISYLLGGLVSEKRMAYLPEDFYEKNRVNLLLNSEVTG
IDTAARQVRIAGGDTIGYDRLLVATGGDPFIPPIEGMADKDRIFTFTTWDDAAKLKGIASDIGRVVVIGGGLIGLKAAEG
LHLIGKQITIVELADRILSAAFDRPAGRVVAKKMKANGIDVITEDTVVRIEGEGAEITGVTLRSGDFIPCDTIIVAIGVR
PACGFLKGSGVEVNRGIVVDDRMETSVEGIYAAGDVAEAKDFFSGVKNPMPIWPDAYIQGDIAGVSMAGGTKAYEGGLAM
NSIELFKVSTISMGITNPAEPKEYEILTYQDLENYQYRKIVIKDGLLAGAVLVGAVDRAGIFSGLIREKIAVVPFKDQLL
APDFGFANLPKDIRTALFAPAGKVTETGRPAVAVGH

Sequences:

>Translated_436_residues
MNYVIIGNSVAAVGAIRGIRSIDQQGNITVISRERHNAYGRPLISYLLGGLVSEKRMAYLPEDFYEKNRVNLLLNSEVTG
IDTAARQVRIAGGDTIGYDRLLVATGGDPFIPPIEGMADKDRIFTFTTWDDAAKLKGIASDIGRVVVIGGGLIGLKAAEG
LHLIGKQITIVELADRILSAAFDRPAGRVVAKKMKANGIDVITEDTVVRIEGEGAEITGVTLRSGDFIPCDTIIVAIGVR
PACGFLKGSGVEVNRGIVVDDRMETSVEGIYAAGDVAEAKDFFSGVKNPMPIWPDAYIQGDIAGVSMAGGTKAYEGGLAM
NSIELFKVSTISMGITNPAEPKEYEILTYQDLENYQYRKIVIKDGLLAGAVLVGAVDRAGIFSGLIREKIAVVPFKDQLL
APDFGFANLPKDIRTALFAPAGKVTETGRPAVAVGH
>Mature_436_residues
MNYVIIGNSVAAVGAIRGIRSIDQQGNITVISRERHNAYGRPLISYLLGGLVSEKRMAYLPEDFYEKNRVNLLLNSEVTG
IDTAARQVRIAGGDTIGYDRLLVATGGDPFIPPIEGMADKDRIFTFTTWDDAAKLKGIASDIGRVVVIGGGLIGLKAAEG
LHLIGKQITIVELADRILSAAFDRPAGRVVAKKMKANGIDVITEDTVVRIEGEGAEITGVTLRSGDFIPCDTIIVAIGVR
PACGFLKGSGVEVNRGIVVDDRMETSVEGIYAAGDVAEAKDFFSGVKNPMPIWPDAYIQGDIAGVSMAGGTKAYEGGLAM
NSIELFKVSTISMGITNPAEPKEYEILTYQDLENYQYRKIVIKDGLLAGAVLVGAVDRAGIFSGLIREKIAVVPFKDQLL
APDFGFANLPKDIRTALFAPAGKVTETGRPAVAVGH

Specific function: Required for nitrite assimilation [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=310, Percent_Identity=28.0645161290323, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI21389617, Length=310, Percent_Identity=28.0645161290323, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI226437568, Length=310, Percent_Identity=28.0645161290323, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI51036596, Length=193, Percent_Identity=28.4974093264249, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI4757732, Length=346, Percent_Identity=23.6994219653179, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI22202629, Length=346, Percent_Identity=23.6994219653179, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI1789065, Length=276, Percent_Identity=28.6231884057971, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789765, Length=408, Percent_Identity=28.1862745098039, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1788892, Length=262, Percent_Identity=27.0992366412214, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI87081763, Length=201, Percent_Identity=31.3432835820896, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786307, Length=271, Percent_Identity=30.6273062730627, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=293, Percent_Identity=29.0102389078498, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI32564386, Length=346, Percent_Identity=26.3005780346821, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24639257, Length=320, Percent_Identity=28.4375, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI281359715, Length=320, Percent_Identity=28.4375, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI281359713, Length=320, Percent_Identity=28.4375, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24639250, Length=320, Percent_Identity=28.4375, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI18543267, Length=320, Percent_Identity=28.4375, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24639252, Length=320, Percent_Identity=28.4375, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24585130, Length=320, Percent_Identity=24.375, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI28573993, Length=350, Percent_Identity=24.8571428571429, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24581020, Length=350, Percent_Identity=24.8571428571429, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24585384, Length=206, Percent_Identity=31.0679611650485, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 46457; Mature: 46457

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYVIIGNSVAAVGAIRGIRSIDQQGNITVISRERHNAYGRPLISYLLGGLVSEKRMAYL
CCEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHCC
PEDFYEKNRVNLLLNSEVTGIDTAARQVRIAGGDTIGYDRLLVATGGDPFIPPIEGMADK
CHHHHCCCCEEEEECCCCCCCCCCHHEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCC
DRIFTFTTWDDAAKLKGIASDIGRVVVIGGGLIGLKAAEGLHLIGKQITIVELADRILSA
CEEEEEEECCCHHHHHHHHHCCCEEEEEECCEEEEECCCCEEECCCEEHHHHHHHHHHHH
AFDRPAGRVVAKKMKANGIDVITEDTVVRIEGEGAEITGVTLRSGDFIPCDTIIVAIGVR
HHCCCCHHHHHHHHCCCCCEEEECCEEEEEECCCCEEEEEEEECCCCCCHHHEEEEECCC
PACGFLKGSGVEVNRGIVVDDRMETSVEGIYAAGDVAEAKDFFSGVKNPMPIWPDAYIQG
CCCCCCCCCCEEECCCEEEECCHHHCCCCEEECCCHHHHHHHHHHCCCCCCCCCCCEEEC
DIAGVSMAGGTKAYEGGLAMNSIELFKVSTISMGITNPAEPKEYEILTYQDLENYQYRKI
CCCEEEECCCCCEECCCEEECCEEEEEEEEEEECCCCCCCCCCEEEEEEECCCCCEEEEE
VIKDGLLAGAVLVGAVDRAGIFSGLIREKIAVVPFKDQLLAPDFGFANLPKDIRTALFAP
EEECCCHHHHHHHHCCHHHHHHHHHHHHHEEEECCCHHHCCCCCCCCCCCHHHHHHHCCC
AGKVTETGRPAVAVGH
CCCCCCCCCCEEEECC
>Mature Secondary Structure
MNYVIIGNSVAAVGAIRGIRSIDQQGNITVISRERHNAYGRPLISYLLGGLVSEKRMAYL
CCEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHCC
PEDFYEKNRVNLLLNSEVTGIDTAARQVRIAGGDTIGYDRLLVATGGDPFIPPIEGMADK
CHHHHCCCCEEEEECCCCCCCCCCHHEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCC
DRIFTFTTWDDAAKLKGIASDIGRVVVIGGGLIGLKAAEGLHLIGKQITIVELADRILSA
CEEEEEEECCCHHHHHHHHHCCCEEEEEECCEEEEECCCCEEECCCEEHHHHHHHHHHHH
AFDRPAGRVVAKKMKANGIDVITEDTVVRIEGEGAEITGVTLRSGDFIPCDTIIVAIGVR
HHCCCCHHHHHHHHCCCCCEEEECCEEEEEECCCCEEEEEEEECCCCCCHHHEEEEECCC
PACGFLKGSGVEVNRGIVVDDRMETSVEGIYAAGDVAEAKDFFSGVKNPMPIWPDAYIQG
CCCCCCCCCCEEECCCEEEECCHHHCCCCEEECCCHHHHHHHHHHCCCCCCCCCCCEEEC
DIAGVSMAGGTKAYEGGLAMNSIELFKVSTISMGITNPAEPKEYEILTYQDLENYQYRKI
CCCEEEECCCCCEECCCEEECCEEEEEEEEEEECCCCCCCCCCEEEEEEECCCCCEEEEE
VIKDGLLAGAVLVGAVDRAGIFSGLIREKIAVVPFKDQLLAPDFGFANLPKDIRTALFAP
EEECCCHHHHHHHHCCHHHHHHHHHHHHHEEEECCCHHHCCCCCCCCCCCHHHHHHHCCC
AGKVTETGRPAVAVGH
CCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]