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The map label for this gene is amtB [C]

Identifier: 39996323

GI number: 39996323

Start: 1324618

End: 1325838

Strand: Reverse

Name: amtB [C]

Synonym: GSU1221

Alternate gene names: 39996323

Gene position: 1325838-1324618 (Counterclockwise)

Preceding gene: 39996335

Following gene: 39996322

Centisome position: 34.76

GC content: 60.52

Gene sequence:

>1221_bases
ATGGACTCTCTGTCAATGGTGACAGGTCTCAGGAACAGTGCCGACGTGCTGTTTCTCATGCTGGGTGCGGTCATGGTCTT
TGCCATGCACGCAGGCTTCGCATTCCTGGAAGTGGGCACCGTCCGCAAGAAAAACCAGGTCAATGCCTTTGTAAAGATCC
TCACCGACTGGTCCGTTTCAACCGTGGCCTATTTTCTGGTGGGATTCCCCATTTCCTACGGAATATCCTTTCTCGTACCT
GCCGGCGACCTTCTTGGCACCACCCAGGGATACGACCTAGTCCGCTACTTTTTCCTGCTCTGTTTCGCAGCATGCATCCC
GGCGATCATCTCGGGCGGAATCGCGGAGCGGGCCAAGTTCTGGCCCCAGGTTTTGGCGGGTGCCATCTTTGCCGGCGTCT
CCTACCCCCTGTTCGAATCCCTGATCTGGGGCCAGAACGCCTCGCTGCTCCAAGGCTTTTTCAAAAGCGTGGGCGGAGCT
GAATTCCACGACTTCGCCGGTTCGGTTGTCGTCCACTCCGTCGGAGGCTGGCTGGCTCTGCCGGCCGTGCTGGTACTGGG
TCCCCGCATGGGGCGCTACGTGCGCGGCAAGTCGCATCCCATCCCCATCAGCAACATACCGTTCCTGGCGCTCGGCTCCT
GGATTCTCGCCGTGGGGTGGTTCGGCTTCAATGTCATGAGCGCGGGGCATGTGGAAAAAATCTCGGGGCTGGTGGCGGTC
AACTCGCTCCTGGCAATGGTCGGCGGCGTACTCGCAGCCATTGTTGCCGGGCGGAATGACCCCGGCTTCGTTCACAACGG
CGCCTTGGCCGGCCTCATCGCCATCTGTGCTGGCAGCGACATCATGCACCCCCTTGCAGCCTTCGCCACCGGTGCCGTTG
CCTCCGTCATCTTCGTCTACGGCTTCCAGTACGAGCAGGAACGGCTCAAGATCGACGACGTACTTGGTGTCTGGCCCCTC
CACGGTGTCATCGGCACCTGGGGCGGCATTGCTGCGGGGATATTCGGTCAGCCGCTGTTCGGAGGCATGGGTGGCGTCTC
CTTCGTTTCCCAATTCCTGGGAAGCCTCGCGGCCGTGGTCTTCGCACTTGTTAACGGTTTCCTGGTCTATGGCATTCTCT
CCCGCACCGTGGGCATCCGGCTAACCGATGAACAGGAGTTTGCCGGCGCCGACCTTTCGATCCACCAGATTGGCGCCTAT
CCCGAAGACCATGTGCGCTAA

Upstream 100 bases:

>100_bases
TTTTACTGAAATTTCACAGCATTATCGATTTGGCAGAGAGGTTGCTATCGAGGGCAAAGACCCAACGAAGGGCATGACCA
CATACCAATGGAGGTAATCA

Downstream 100 bases:

>100_bases
CCTCACCTGACATACCGAACAAAAGGCCCTACCGGGGCCTTTTGTTTTACAGCCATCACCCCCCGAATTTTTGACAACAT
TTCTAAAGTATTCTCTTTAC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MDSLSMVTGLRNSADVLFLMLGAVMVFAMHAGFAFLEVGTVRKKNQVNAFVKILTDWSVSTVAYFLVGFPISYGISFLVP
AGDLLGTTQGYDLVRYFFLLCFAACIPAIISGGIAERAKFWPQVLAGAIFAGVSYPLFESLIWGQNASLLQGFFKSVGGA
EFHDFAGSVVVHSVGGWLALPAVLVLGPRMGRYVRGKSHPIPISNIPFLALGSWILAVGWFGFNVMSAGHVEKISGLVAV
NSLLAMVGGVLAAIVAGRNDPGFVHNGALAGLIAICAGSDIMHPLAAFATGAVASVIFVYGFQYEQERLKIDDVLGVWPL
HGVIGTWGGIAAGIFGQPLFGGMGGVSFVSQFLGSLAAVVFALVNGFLVYGILSRTVGIRLTDEQEFAGADLSIHQIGAY
PEDHVR

Sequences:

>Translated_406_residues
MDSLSMVTGLRNSADVLFLMLGAVMVFAMHAGFAFLEVGTVRKKNQVNAFVKILTDWSVSTVAYFLVGFPISYGISFLVP
AGDLLGTTQGYDLVRYFFLLCFAACIPAIISGGIAERAKFWPQVLAGAIFAGVSYPLFESLIWGQNASLLQGFFKSVGGA
EFHDFAGSVVVHSVGGWLALPAVLVLGPRMGRYVRGKSHPIPISNIPFLALGSWILAVGWFGFNVMSAGHVEKISGLVAV
NSLLAMVGGVLAAIVAGRNDPGFVHNGALAGLIAICAGSDIMHPLAAFATGAVASVIFVYGFQYEQERLKIDDVLGVWPL
HGVIGTWGGIAAGIFGQPLFGGMGGVSFVSQFLGSLAAVVFALVNGFLVYGILSRTVGIRLTDEQEFAGADLSIHQIGAY
PEDHVR
>Mature_406_residues
MDSLSMVTGLRNSADVLFLMLGAVMVFAMHAGFAFLEVGTVRKKNQVNAFVKILTDWSVSTVAYFLVGFPISYGISFLVP
AGDLLGTTQGYDLVRYFFLLCFAACIPAIISGGIAERAKFWPQVLAGAIFAGVSYPLFESLIWGQNASLLQGFFKSVGGA
EFHDFAGSVVVHSVGGWLALPAVLVLGPRMGRYVRGKSHPIPISNIPFLALGSWILAVGWFGFNVMSAGHVEKISGLVAV
NSLLAMVGGVLAAIVAGRNDPGFVHNGALAGLIAICAGSDIMHPLAAFATGAVASVIFVYGFQYEQERLKIDDVLGVWPL
HGVIGTWGGIAAGIFGQPLFGGMGGVSFVSQFLGSLAAVVFALVNGFLVYGILSRTVGIRLTDEQEFAGADLSIHQIGAY
PEDHVR

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=430, Percent_Identity=29.0697674418605, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17550180, Length=447, Percent_Identity=29.5302013422819, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17550182, Length=432, Percent_Identity=28.9351851851852, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI32564042, Length=422, Percent_Identity=29.3838862559242, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI133931100, Length=435, Percent_Identity=27.3563218390805, Blast_Score=112, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6321559, Length=439, Percent_Identity=23.6902050113895, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6325396, Length=436, Percent_Identity=23.1651376146789, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI161078314, Length=418, Percent_Identity=29.6650717703349, Blast_Score=137, Evalue=9e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 42844; Mature: 42844

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSLSMVTGLRNSADVLFLMLGAVMVFAMHAGFAFLEVGTVRKKNQVNAFVKILTDWSVS
CCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCHHHEECCCCHHHHHHHHHHHHHHCCCHH
TVAYFLVGFPISYGISFLVPAGDLLGTTQGYDLVRYFFLLCFAACIPAIISGGIAERAKF
HHHHHHHHCCHHHCCCEEECCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
WPQVLAGAIFAGVSYPLFESLIWGQNASLLQGFFKSVGGAEFHDFAGSVVVHSVGGWLAL
HHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHCHHHHHHHCHHHHH
PAVLVLGPRMGRYVRGKSHPIPISNIPFLALGSWILAVGWFGFNVMSAGHVEKISGLVAV
HHHHHHCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
NSLLAMVGGVLAAIVAGRNDPGFVHNGALAGLIAICAGSDIMHPLAAFATGAVASVIFVY
HHHHHHHHHHHHHHHHCCCCCCCEECHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
GFQYEQERLKIDDVLGVWPLHGVIGTWGGIAAGIFGQPLFGGMGGVSFVSQFLGSLAAVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHH
FALVNGFLVYGILSRTVGIRLTDEQEFAGADLSIHQIGAYPEDHVR
HHHHHHHHHHHHHHHHHCCEECCCHHHCCCCCCHHHCCCCCHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHCCEECCCHHHCCCCCCHHHCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]