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The map label for this gene is zraS [H]

Identifier: 39996140

GI number: 39996140

Start: 1124101

End: 1125642

Strand: Direct

Name: zraS [H]

Synonym: GSU1038

Alternate gene names: 39996140

Gene position: 1124101-1125642 (Clockwise)

Preceding gene: 39996139

Following gene: 39996141

Centisome position: 29.47

GC content: 62.71

Gene sequence:

>1542_bases
ATGACCACTCACGTGCGGATGAAACATGTTATGGATGAATGTGCTCTGGAAAATGCACCTCCTCTGATCCTCCTGGTGGA
CGATGATGCGGTGACCCGCAAGATGCTGCGCAACCTCTTCACGCTTTCGGGATACCGCGTGGCCGACGCGGAAGACGGCG
CCCGGGCGGTTGAGATGTTTCGCGAACTTTCACCGGATCTGGTCCTTCTCGATATCATGATGCCGGTCATGGACGGGTAT
GGCGCCTGTGCGGCGATCCGCGGCCTGCCGGGCGGCGAGCATGTCCCGATCATCATCATGACTGCCCTGGACGATGCTAA
CTCAATCGGCCGTGCCTTTGACGCCGGGGCCACCGATTTCATCGAGAAGCCCGTGAACTGGATGCTTCTCAACCACCGCC
TTCCCTATCTGCTGCGGGCGCGGGACGCCTTTGTCTCCCTCCAGCGCAGCGAGGCTACCAGCCGCCTGCTTTCGGGCGAG
CTGATGGCGCTCCTGAACTCAATCAACGACAGCCTTGTCCTGTTCTCCACCGATCTGAAACTGCTCTGGGCCAACCGGAG
CGCCGAGCATCTCTACGGCGATTACGTCGAGCAGCTCGTTGGTCAGGAGTTCACTTCCCTCAAGGGGGCGCGGGGCATCC
CGTCCGATGCCATTGCCGTACGCGCGTCGCTCGGCTCGGGCGAGCCCTGCTACGAGCGCGTATCAGCCGCCGACGGCAGG
ATCTGGGACATGAAGTATTTCCCCGTGCGCGGCGAAGACGGCACCGTGCGCGGCATCATCGAGCTCGCCTCGGACATGAC
GGAGGTGGTCTCACTCCAGGCGGAGGCCCTCCGGTCGGGACAACTGGCAGCCCTGGGCGAACTGGCAGCCGGCGTGGCCC
ACGAGATCAACAACCCCATCAACGGCATCATCAACTATGCGCAACTGCTGGTGAACTGGCTGCCGTCCGCCTGCAAGGAG
CGCGACATCGCCGAACGGATCATCCGGGAGGGAGATCGGGTGGCGGGGATCGTCCGGGGGCTTCTCTTCTTTGCCCGGGA
GGGCATGGGGGCGCGCCTGCCCTGCAATGTCGCCGACGTCCTGACCGACACGCTCACCCTCACCGAGGCCCAGATCCGCA
AGGACGGCATTACTCTCAAGGTCGGGGTGCCGGCTGACCTGGGTCGCGTGAGAGCCAGCCACCAGCAGCTCCAGCAGGTC
TTCCTCAATATTATCAGCAACGCCCGCTATGCGCTCAACGAGAAGTTCCGCGGTTTCCATTCCGCCAAGATCCTGGAGAT
CCGGGGCGAGCGCGTGCTCATCGATGACCGGCCGTACATCCGGATCGGCTTCAACGATACCGGTACCGGTATCCCGGAAG
CCATCAAGGACAAGGTGATGACGCCGTTTTTTTCGACCAAGCCTACCTGCAAGGGGACAGGCCTGGGGCTCAGCATCAGC
CAGAACATCATCCGCGACCATGACGGCAACCTCTCCATCGAGAGCCGCGAGGGGGAATTCACCCTCGTGAGCATCGACCT
TCCCGCCGAGGAGACGCCATGA

Upstream 100 bases:

>100_bases
TTGCGTCGAATCTCCCGATTTGACAAAAAAATGTTTCCGAGCCGTTGGGAGGTTGAAAAAAATCTAAATTCACCGATACT
TATGACCGATACGGTAAAGT

Downstream 100 bases:

>100_bases
CATACAGCATTCTCGTGGTAGACGACGAGGAAAGCCTTCGCTACACCTTCCGCTGCTTCCTGGAGCATCAGGGCTATGAG
GTAGCGATTGCGGCGAACTA

Product: sensory box histidine kinase/response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MTTHVRMKHVMDECALENAPPLILLVDDDAVTRKMLRNLFTLSGYRVADAEDGARAVEMFRELSPDLVLLDIMMPVMDGY
GACAAIRGLPGGEHVPIIIMTALDDANSIGRAFDAGATDFIEKPVNWMLLNHRLPYLLRARDAFVSLQRSEATSRLLSGE
LMALLNSINDSLVLFSTDLKLLWANRSAEHLYGDYVEQLVGQEFTSLKGARGIPSDAIAVRASLGSGEPCYERVSAADGR
IWDMKYFPVRGEDGTVRGIIELASDMTEVVSLQAEALRSGQLAALGELAAGVAHEINNPINGIINYAQLLVNWLPSACKE
RDIAERIIREGDRVAGIVRGLLFFAREGMGARLPCNVADVLTDTLTLTEAQIRKDGITLKVGVPADLGRVRASHQQLQQV
FLNIISNARYALNEKFRGFHSAKILEIRGERVLIDDRPYIRIGFNDTGTGIPEAIKDKVMTPFFSTKPTCKGTGLGLSIS
QNIIRDHDGNLSIESREGEFTLVSIDLPAEETP

Sequences:

>Translated_513_residues
MTTHVRMKHVMDECALENAPPLILLVDDDAVTRKMLRNLFTLSGYRVADAEDGARAVEMFRELSPDLVLLDIMMPVMDGY
GACAAIRGLPGGEHVPIIIMTALDDANSIGRAFDAGATDFIEKPVNWMLLNHRLPYLLRARDAFVSLQRSEATSRLLSGE
LMALLNSINDSLVLFSTDLKLLWANRSAEHLYGDYVEQLVGQEFTSLKGARGIPSDAIAVRASLGSGEPCYERVSAADGR
IWDMKYFPVRGEDGTVRGIIELASDMTEVVSLQAEALRSGQLAALGELAAGVAHEINNPINGIINYAQLLVNWLPSACKE
RDIAERIIREGDRVAGIVRGLLFFAREGMGARLPCNVADVLTDTLTLTEAQIRKDGITLKVGVPADLGRVRASHQQLQQV
FLNIISNARYALNEKFRGFHSAKILEIRGERVLIDDRPYIRIGFNDTGTGIPEAIKDKVMTPFFSTKPTCKGTGLGLSIS
QNIIRDHDGNLSIESREGEFTLVSIDLPAEETP
>Mature_512_residues
TTHVRMKHVMDECALENAPPLILLVDDDAVTRKMLRNLFTLSGYRVADAEDGARAVEMFRELSPDLVLLDIMMPVMDGYG
ACAAIRGLPGGEHVPIIIMTALDDANSIGRAFDAGATDFIEKPVNWMLLNHRLPYLLRARDAFVSLQRSEATSRLLSGEL
MALLNSINDSLVLFSTDLKLLWANRSAEHLYGDYVEQLVGQEFTSLKGARGIPSDAIAVRASLGSGEPCYERVSAADGRI
WDMKYFPVRGEDGTVRGIIELASDMTEVVSLQAEALRSGQLAALGELAAGVAHEINNPINGIINYAQLLVNWLPSACKER
DIAERIIREGDRVAGIVRGLLFFAREGMGARLPCNVADVLTDTLTLTEAQIRKDGITLKVGVPADLGRVRASHQQLQQVF
LNIISNARYALNEKFRGFHSAKILEIRGERVLIDDRPYIRIGFNDTGTGIPEAIKDKVMTPFFSTKPTCKGTGLGLSISQ
NIIRDHDGNLSIESREGEFTLVSIDLPAEETP

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=349, Percent_Identity=30.0859598853868, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1790436, Length=241, Percent_Identity=30.2904564315353, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1788549, Length=263, Percent_Identity=30.4182509505703, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788550, Length=109, Percent_Identity=37.6146788990826, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI48994928, Length=371, Percent_Identity=22.911051212938, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789149, Length=220, Percent_Identity=24.5454545454545, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790437, Length=105, Percent_Identity=38.0952380952381, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787487, Length=103, Percent_Identity=33.9805825242718, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI87082012, Length=174, Percent_Identity=28.1609195402299, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI87081816, Length=242, Percent_Identity=27.6859504132231, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI145693157, Length=133, Percent_Identity=32.3308270676692, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 56272; Mature: 56141

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTHVRMKHVMDECALENAPPLILLVDDDAVTRKMLRNLFTLSGYRVADAEDGARAVEMF
CCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHH
RELSPDLVLLDIMMPVMDGYGACAAIRGLPGGEHVPIIIMTALDDANSIGRAFDAGATDF
HHCCCCEEHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCHHH
IEKPVNWMLLNHRLPYLLRARDAFVSLQRSEATSRLLSGELMALLNSINDSLVLFSTDLK
HHCCHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEE
LLWANRSAEHLYGDYVEQLVGQEFTSLKGARGIPSDAIAVRASLGSGEPCYERVSAADGR
EEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCE
IWDMKYFPVRGEDGTVRGIIELASDMTEVVSLQAEALRSGQLAALGELAAGVAHEINNPI
EEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHH
NGIINYAQLLVNWLPSACKERDIAERIIREGDRVAGIVRGLLFFAREGMGARLPCNVADV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
LTDTLTLTEAQIRKDGITLKVGVPADLGRVRASHQQLQQVFLNIISNARYALNEKFRGFH
HHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
SAKILEIRGERVLIDDRPYIRIGFNDTGTGIPEAIKDKVMTPFFSTKPTCKGTGLGLSIS
CEEEEEECCCEEEECCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHH
QNIIRDHDGNLSIESREGEFTLVSIDLPAEETP
HHHHHCCCCCEEEECCCCCEEEEEEECCCCCCC
>Mature Secondary Structure 
TTHVRMKHVMDECALENAPPLILLVDDDAVTRKMLRNLFTLSGYRVADAEDGARAVEMF
CCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHH
RELSPDLVLLDIMMPVMDGYGACAAIRGLPGGEHVPIIIMTALDDANSIGRAFDAGATDF
HHCCCCEEHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCHHH
IEKPVNWMLLNHRLPYLLRARDAFVSLQRSEATSRLLSGELMALLNSINDSLVLFSTDLK
HHCCHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEE
LLWANRSAEHLYGDYVEQLVGQEFTSLKGARGIPSDAIAVRASLGSGEPCYERVSAADGR
EEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCE
IWDMKYFPVRGEDGTVRGIIELASDMTEVVSLQAEALRSGQLAALGELAAGVAHEINNPI
EEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHH
NGIINYAQLLVNWLPSACKERDIAERIIREGDRVAGIVRGLLFFAREGMGARLPCNVADV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
LTDTLTLTEAQIRKDGITLKVGVPADLGRVRASHQQLQQVFLNIISNARYALNEKFRGFH
HHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
SAKILEIRGERVLIDDRPYIRIGFNDTGTGIPEAIKDKVMTPFFSTKPTCKGTGLGLSIS
CEEEEEECCCEEEECCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHH
QNIIRDHDGNLSIESREGEFTLVSIDLPAEETP
HHHHHCCCCCEEEECCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]