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The map label for this gene is zraS [H]

Identifier: 39996044

GI number: 39996044

Start: 1011471

End: 1012217

Strand: Direct

Name: zraS [H]

Synonym: GSU0941

Alternate gene names: 39996044

Gene position: 1011471-1012217 (Clockwise)

Preceding gene: 39996043

Following gene: 39996045

Centisome position: 26.52

GC content: 61.31

Gene sequence:

>747_bases
ATGGCAAATGCGCTTGCGAAGTACATAGCAGAAAAGGACCCTGTCGAGGGCGAGCTGCTGGAGCTTCTTGCATTCAACGA
GAAGATGGCCGAGCTCGGCCGGATGGCGGCCGGTGTGGTGCATGAGCTGAACACGCCTCTGTCGGTCATCGTTTCGGCCA
CCCAGATGATCCTCCGCGAAGAGGGCCTGCCCGATTTCGTACGCGAGATGGTGGAGCGGGTCAACCAGGAAGCCCACCGG
CTGTCTGACATGACCAAGGGGGTCCTTTCCTTTACCCGCCGGGACGTAAGCGACCACCAGGAAGCGGACGTGAACCAGGT
GCTCCGGGAGGCCATGACCCTGTTGCGTTACGAGGCCCATAAACGCTCTATCGCCGTCATCGAAGATCTGGATTACCGGC
TCCCCTTCATTGCCGCCGACGCCAACCGGGCGAAGCAGGTTATCATAAACCTGGTCATGAACGCCCTCCAGGCCATGGGA
GAGGGCGGAAAGCTCTTCATTCGCACCAGCCAGAACGGGGAGGGGGGCGTAACGGTGCAGGTGACGGATACGGGTCCCGG
CATCCCGGCTGAATTGCTGGGGCATATCTTCACCCCCTTTTTCACCACCAAAGAGCCGGGAGAGGGCACCGGGCTGGGCC
TGTTCATTACCCGCAAGCTCATGGAGTCGGTGGGCGGGGCCATCGCGGTGGAAAGCACGGTGGGGGAGGGGACATCCTTT
ACGCTCACCTTTGTCGCAGCCGAGTGA

Upstream 100 bases:

>100_bases
GTAAAGGCCTGAATATATAACATCTTCGTTCTGGCATGCCTCGTGCCTAACGGATGGTGGGGCTCTCCGCTTCGGCCCCC
AACCGGATCCCGGGTGACCC

Downstream 100 bases:

>100_bases
ATTTTTAGGCACTTCCCGATTCACAGCCGCCCAACTACGGTTCGGGCGGCTTTTTTTGTCGCGTGAAACGCGGCTGTTTG
CTATAACCCCTCAAGCACCA

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MANALAKYIAEKDPVEGELLELLAFNEKMAELGRMAAGVVHELNTPLSVIVSATQMILREEGLPDFVREMVERVNQEAHR
LSDMTKGVLSFTRRDVSDHQEADVNQVLREAMTLLRYEAHKRSIAVIEDLDYRLPFIAADANRAKQVIINLVMNALQAMG
EGGKLFIRTSQNGEGGVTVQVTDTGPGIPAELLGHIFTPFFTTKEPGEGTGLGLFITRKLMESVGGAIAVESTVGEGTSF
TLTFVAAE

Sequences:

>Translated_248_residues
MANALAKYIAEKDPVEGELLELLAFNEKMAELGRMAAGVVHELNTPLSVIVSATQMILREEGLPDFVREMVERVNQEAHR
LSDMTKGVLSFTRRDVSDHQEADVNQVLREAMTLLRYEAHKRSIAVIEDLDYRLPFIAADANRAKQVIINLVMNALQAMG
EGGKLFIRTSQNGEGGVTVQVTDTGPGIPAELLGHIFTPFFTTKEPGEGTGLGLFITRKLMESVGGAIAVESTVGEGTSF
TLTFVAAE
>Mature_247_residues
ANALAKYIAEKDPVEGELLELLAFNEKMAELGRMAAGVVHELNTPLSVIVSATQMILREEGLPDFVREMVERVNQEAHRL
SDMTKGVLSFTRRDVSDHQEADVNQVLREAMTLLRYEAHKRSIAVIEDLDYRLPFIAADANRAKQVIINLVMNALQAMGE
GGKLFIRTSQNGEGGVTVQVTDTGPGIPAELLGHIFTPFFTTKEPGEGTGLGLFITRKLMESVGGAIAVESTVGEGTSFT
LTFVAAE

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=219, Percent_Identity=36.5296803652968, Blast_Score=137, Evalue=9e-34,
Organism=Escherichia coli, GI1788549, Length=222, Percent_Identity=34.2342342342342, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1790300, Length=223, Percent_Identity=32.7354260089686, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI48994928, Length=222, Percent_Identity=28.8288288288288, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789149, Length=218, Percent_Identity=29.8165137614679, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI145693157, Length=226, Percent_Identity=29.2035398230088, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87081816, Length=213, Percent_Identity=28.6384976525822, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786600, Length=226, Percent_Identity=29.646017699115, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790346, Length=217, Percent_Identity=25.3456221198157, Blast_Score=67, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 26989; Mature: 26858

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANALAKYIAEKDPVEGELLELLAFNEKMAELGRMAAGVVHELNTPLSVIVSATQMILRE
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
EGLPDFVREMVERVNQEAHRLSDMTKGVLSFTRRDVSDHQEADVNQVLREAMTLLRYEAH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHH
KRSIAVIEDLDYRLPFIAADANRAKQVIINLVMNALQAMGEGGKLFIRTSQNGEGGVTVQ
HHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEE
VTDTGPGIPAELLGHIFTPFFTTKEPGEGTGLGLFITRKLMESVGGAIAVESTVGEGTSF
EECCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCEE
TLTFVAAE
EEEEEECC
>Mature Secondary Structure 
ANALAKYIAEKDPVEGELLELLAFNEKMAELGRMAAGVVHELNTPLSVIVSATQMILRE
CHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
EGLPDFVREMVERVNQEAHRLSDMTKGVLSFTRRDVSDHQEADVNQVLREAMTLLRYEAH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHH
KRSIAVIEDLDYRLPFIAADANRAKQVIINLVMNALQAMGEGGKLFIRTSQNGEGGVTVQ
HHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEE
VTDTGPGIPAELLGHIFTPFFTTKEPGEGTGLGLFITRKLMESVGGAIAVESTVGEGTSF
EECCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCEE
TLTFVAAE
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]