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The map label for this gene is amtB [C]

Identifier: 39996043

GI number: 39996043

Start: 1009771

End: 1011240

Strand: Direct

Name: amtB [C]

Synonym: GSU0940

Alternate gene names: 39996043

Gene position: 1009771-1011240 (Clockwise)

Preceding gene: 39996042

Following gene: 39996044

Centisome position: 26.47

GC content: 65.17

Gene sequence:

>1470_bases
ATGAAGTGGAAACGTTCGATCATCACACTTGCCCTGGCCGCCGCAGCCTTCATGGCTCCGGCCGCAGTCCTCGCCGAAGA
GGCCAAGGCCCCCGCCGTGGCGGCAACCCAGGCAGCCGTCACCTCCGCCCAGGCTGCTCCGGCGGCCGTTGCCCCGGCTG
AGGCCGCACCGGCGGCTGCCGAAGCCCCCAAGGTGGCCGAACCGGTCCTCAATACCGGCAATACCGCCTGGATGCTCGTC
TGCGCGGCCCTGGTCCTTCTGATGCTTCCCGGCCTTGCCCTCTTTTACGGCGGCATGGTCCGGAGCAAAAACGTCCTCTC
AACCCTGATGCATTCCTTTGTTGCCATGGGGATCGTGGGGGTCCAGTGGGCCGTCATCGGTTACTCGCTGGCCTTCGGTC
CCGACATGGGCGGCATGGGGCTTGTGGGAGACTTCAGCCGGGCGCTGCTCAACGGAATGATCAGCTTCAAGGACGGCGCG
CCGGTCTACACCCTCTGGCAGAACGTGACCAGTGAACCCAACGCCATCCCCGAGTACGTCTTTGCCATGTTCCAGGGAAT
GTTCGCCATGATCACCGTAGCCCTCATCTCCGGCGCCATGGCCGAGCGGGTTAAGTTCTCCGCCTACTGCGTCTTTGTGC
TCCTTTGGACCACCCTGGTCTACGATCCCCTGGCCCACTGGGTCTGGATGGTGGACGGCTGGCTCTTCAAGAAGGGTGCC
CTGGACTTTGCCGGCGGCACCGTTGTTCACCTCTCCTCCGGTATTTCCGCCCTGGCGTTCCTCGTCTTCCTCGGCAAACG
TCACGGGTTCCCCCATGAGCGTATGGCTCCCCATAACCTCTCCATGACCATGCTCGGCGTGGGGCTCCTCTGGTTCGGCT
GGTTCGGGTTCAACGCGGGTTCGGCGGTAGTCGGCGTGAACAACGTTGATGCGGCCGGCGGGCTTGCGGGCCTCGCCTTC
GCCACCACCACCATCGCCCCGGCAGCCGGCGGCCTGGCCTGGATGATCGCCGAATGGATCCACGCGGGCAGGCCCTCGGC
ACTCGGCTTCGGTTCAGGCGTGGTCGCAGGACTGGTCGGCATTACGCCCGCGGCCGGCTTTGTCCAGCCGGGCGCTGCCA
TCATCATCGGCCTCGGCGCCGGCGTGATCTGCTACCTGGGTGTGCTCATGAAGGCGAAGCTCAAGTATGACGACTCCCTG
GATGCCTTCGGCGTGCACGGCGTGGGCGGCACCTTTGGCGCCCTCGTCACCGGCGTCTTCGCCACGGTGGGGGCCACGGG
GCTCCTCTCCGGCAACGTGTCCCAGTTCATCACCCAGGTGATCGGGGTGGTGTCGGCCGGTGCCTATGCCTTCATCGTGA
CCCTGGTCATCGCCTTCATCCTCGACAAGACCATCGGTCTCCGGGTGGAGAAGGAAGACGAGATCATGGGCCTCGACCAG
ACCCAGCACTCCGAGTCGGCGTACAACTAG

Upstream 100 bases:

>100_bases
ATCCTTTCCCTGGACGAGGCGGTCAGGATCAGGACCGGTGAGAAGGGTAACGAAGCGATCTGATCGCTCGAGATATTACA
GAAACCAACGGAGGTTCATC

Downstream 100 bases:

>100_bases
AAACATTTTATCTCACGTTTCTGATGAGCCGCCCCTTCCAGGGGGCGGCTTTTCTTTTTGTGCTGAAATATTGGGCAGAC
TGGTGATTGAAAAAGCGGCA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MKWKRSIITLALAAAAFMAPAAVLAEEAKAPAVAATQAAVTSAQAAPAAVAPAEAAPAAAEAPKVAEPVLNTGNTAWMLV
CAALVLLMLPGLALFYGGMVRSKNVLSTLMHSFVAMGIVGVQWAVIGYSLAFGPDMGGMGLVGDFSRALLNGMISFKDGA
PVYTLWQNVTSEPNAIPEYVFAMFQGMFAMITVALISGAMAERVKFSAYCVFVLLWTTLVYDPLAHWVWMVDGWLFKKGA
LDFAGGTVVHLSSGISALAFLVFLGKRHGFPHERMAPHNLSMTMLGVGLLWFGWFGFNAGSAVVGVNNVDAAGGLAGLAF
ATTTIAPAAGGLAWMIAEWIHAGRPSALGFGSGVVAGLVGITPAAGFVQPGAAIIIGLGAGVICYLGVLMKAKLKYDDSL
DAFGVHGVGGTFGALVTGVFATVGATGLLSGNVSQFITQVIGVVSAGAYAFIVTLVIAFILDKTIGLRVEKEDEIMGLDQ
TQHSESAYN

Sequences:

>Translated_489_residues
MKWKRSIITLALAAAAFMAPAAVLAEEAKAPAVAATQAAVTSAQAAPAAVAPAEAAPAAAEAPKVAEPVLNTGNTAWMLV
CAALVLLMLPGLALFYGGMVRSKNVLSTLMHSFVAMGIVGVQWAVIGYSLAFGPDMGGMGLVGDFSRALLNGMISFKDGA
PVYTLWQNVTSEPNAIPEYVFAMFQGMFAMITVALISGAMAERVKFSAYCVFVLLWTTLVYDPLAHWVWMVDGWLFKKGA
LDFAGGTVVHLSSGISALAFLVFLGKRHGFPHERMAPHNLSMTMLGVGLLWFGWFGFNAGSAVVGVNNVDAAGGLAGLAF
ATTTIAPAAGGLAWMIAEWIHAGRPSALGFGSGVVAGLVGITPAAGFVQPGAAIIIGLGAGVICYLGVLMKAKLKYDDSL
DAFGVHGVGGTFGALVTGVFATVGATGLLSGNVSQFITQVIGVVSAGAYAFIVTLVIAFILDKTIGLRVEKEDEIMGLDQ
TQHSESAYN
>Mature_489_residues
MKWKRSIITLALAAAAFMAPAAVLAEEAKAPAVAATQAAVTSAQAAPAAVAPAEAAPAAAEAPKVAEPVLNTGNTAWMLV
CAALVLLMLPGLALFYGGMVRSKNVLSTLMHSFVAMGIVGVQWAVIGYSLAFGPDMGGMGLVGDFSRALLNGMISFKDGA
PVYTLWQNVTSEPNAIPEYVFAMFQGMFAMITVALISGAMAERVKFSAYCVFVLLWTTLVYDPLAHWVWMVDGWLFKKGA
LDFAGGTVVHLSSGISALAFLVFLGKRHGFPHERMAPHNLSMTMLGVGLLWFGWFGFNAGSAVVGVNNVDAAGGLAGLAF
ATTTIAPAAGGLAWMIAEWIHAGRPSALGFGSGVVAGLVGITPAAGFVQPGAAIIIGLGAGVICYLGVLMKAKLKYDDSL
DAFGVHGVGGTFGALVTGVFATVGATGLLSGNVSQFITQVIGVVSAGAYAFIVTLVIAFILDKTIGLRVEKEDEIMGLDQ
TQHSESAYN

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=434, Percent_Identity=44.0092165898618, Blast_Score=309, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI17550180, Length=466, Percent_Identity=29.3991416309013, Blast_Score=147, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17550182, Length=459, Percent_Identity=30.0653594771242, Blast_Score=142, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI133931100, Length=469, Percent_Identity=25.1599147121535, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI32564042, Length=444, Percent_Identity=26.1261261261261, Blast_Score=119, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6324187, Length=439, Percent_Identity=37.8132118451025, Blast_Score=265, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6325396, Length=431, Percent_Identity=33.1786542923434, Blast_Score=236, Evalue=5e-63,
Organism=Saccharomyces cerevisiae, GI6321559, Length=426, Percent_Identity=31.2206572769953, Blast_Score=216, Evalue=7e-57,
Organism=Drosophila melanogaster, GI161078314, Length=432, Percent_Identity=30.5555555555556, Blast_Score=147, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 50518; Mature: 50518

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCC
>Mature Secondary Structure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CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]