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The map label for this gene is ligA

Identifier: 39995996

GI number: 39995996

Start: 949753

End: 951765

Strand: Reverse

Name: ligA

Synonym: GSU0890

Alternate gene names: 39995996

Gene position: 951765-949753 (Counterclockwise)

Preceding gene: 308513291

Following gene: 39995995

Centisome position: 24.95

GC content: 67.01

Gene sequence:

>2013_bases
ATGGACAAGCAGACCGCCGCGGACCGCGTTGCCGCACTCCGCACTGAACTTGAGCGCCACAACCGCCTCTACTACGCGGA
AGACCGCCCCGAGATCACCGACGCCGAATACGATCTCCTCTTCCGCGAACTGGTGGACCTGGAAACGCGGTTCCCCGACC
TGGCCGCTCCCGACTCCCCAACGCAACGGGTGGGCGGCGCGCCCCTGGACAAGTTCGAGCAGGTCACCCACCGCATCCCC
ATGCTCTCCCTGGAAAACGCCTTTACGGACGTCGAGATCGCTGACTTCGACGACCGGGTCAAACGCTTCCTGGGGCTCCA
CGGCGACGTGGAAATCGATTACGTCTGCGAACCCAAGATGGATGGGCTTGCCGTGGAACTGGTCTACGAGCGCGGCATCC
TCACCGTCGGCTCCACCCGGGGCGACGGCGTGGTGGGGGAGAACGTCACCCACAACCTGAAGACCGTCCGGGGCATCCCC
CTCCGACTCCGGTGCGAGCAGCCGCCGGAATTGCTGGAGGTGCGGGGCGAGGTTTACCTCCCCCTGGCCGCGTTCCAGCG
GCTGAACGCCCAGCGGGAGGAGGAGGGGGAGCCACCCTTTGCCAACCCCCGTAACGCCGCGGCCGGCTCCATCCGCCAGC
TGGACTCCCGGATCACGGCCCGGCGCCCCCTGGCCATCTTCTGCTACGCGCCGGGCGAGGTCCGGGGCGCCTCCTTCGCG
GCACAGACTGAATTTCTCGACCGGATCGGCGAATGGGGCCTGCCGGTCAATCCCCTGATCCGGCCGGTCAAGGGAGTGGC
GGAGATCCTCGCCTACTACCGGGAGATGACCGAACGCCGCGACTCGCTCCCCTACGAGATCGACGGCGTGGTGGTCAAGG
TGGATTCCTTTGCCCTCCAGCGGGAGCTGGGAGAGAAGAGCCGCTCCCCCCGCTGGGCCGTGGCCGTCAAGTTCCCGCCC
CGGCAGGCGGTGACCGTGGTGGAGGACATCGTCCCCTCAGTGGGGCGCACCGGGGTGATCACCCCCACGGCCAACCTCAG
GCCCGTGGAGGTCTCCGGCGTCACCGTCTCCCGCGCCACCCTCCACAACTGGGAGGAGATGGAGCGCAAGGACATCCGCA
TCGGCGACACCGTGGTGATCGAGCGGGCCGGCGACGTGATCCCGGCCGTGGTCAAGGTCCTGACCGAAAAGCGTTCGGGC
AGCGAACGCCCCCTTCCCATCCCGGCCGTCTGCCCCGAGTGCGGCTCCGAGGTGGTGAAGATCCCCGACGAGGTGGCGGT
CCGCTGCATGGGGCTCTCCTGCCCGGCCCAGATCCGTGAATCCATCATCCACTTCGCCTCCCGCGACGCCATGGACATGG
AGGGGCTGGGCGAGAAATACATCGAACAGCTCCTGCGGCTGGGCCTGGTAAAGAACGTGGCCGACCTCTACACCCTCACC
AAAGACCAGTTCATGCAGTTCGACCGGATGGGGGACAAGCTGGCCGAAAACCTCCTGAACGCCATCGAGGCCAGCAAGCA
ACGGGAGCTCTCCCGCTTCATCTTCGCCCTGGGCATCCGCCACGTGGGGGAACACACGGCAAAACTCCTGGCCGGCGCCT
TCGGCAGCATCGAAAACCTGGAGCGGGCCACCGAGGAGGAACTCCTCTCCATCCGCGAGGTGGGCCCCCAGGTTGCCCGG
AGCATCATCACCTTCTTCCGCAACGAGGGGAACCGGGAGACCATCCGGCGGATGTTCAAGGCCGGGGTCCGGCCCACGGC
GGAGGAGAAAAAGGTGGGGGGCCGCTTCACCGGCAAGACCTTCGTCTTCACCGGGGCGCTCACCCGCTTCAGCCGCCCTG
AGGCCCAGAAGATGGTAGAGAAGGAGGGGGGCCACGCGGCCGGGTCCGTCTCGAAAAAGACCGACTACGTGGTTGCCGGC
GCCGAGGCCGGCAGCAAGCTGGACAAGGCCCGCCAGCTGGGAGTCAGGGTGCTGACCGAGGATGAATTCCTGGCAATGCT
GGAGGAGGGATAG

Upstream 100 bases:

>100_bases
AACCGCGAGGCCCTAATGCCCCGTCCCGGCCGGGAGCGCAAATTGCCATCCGGGTGCCGCTGTGCTATAAGCTCCTCTAT
CCCCACGCCACGGAGCCCCC

Downstream 100 bases:

>100_bases
ATGAAGATCCGCGCCATCGTGACCATCAAGGGGCTGGTGCAGGGGGTGGCCTTCCGGCACCACACGGTGCAGCAGGCCCA
GCGGCTCGGCGTATCCGGCT

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 670; Mature: 670

Protein sequence:

>670_residues
MDKQTAADRVAALRTELERHNRLYYAEDRPEITDAEYDLLFRELVDLETRFPDLAAPDSPTQRVGGAPLDKFEQVTHRIP
MLSLENAFTDVEIADFDDRVKRFLGLHGDVEIDYVCEPKMDGLAVELVYERGILTVGSTRGDGVVGENVTHNLKTVRGIP
LRLRCEQPPELLEVRGEVYLPLAAFQRLNAQREEEGEPPFANPRNAAAGSIRQLDSRITARRPLAIFCYAPGEVRGASFA
AQTEFLDRIGEWGLPVNPLIRPVKGVAEILAYYREMTERRDSLPYEIDGVVVKVDSFALQRELGEKSRSPRWAVAVKFPP
RQAVTVVEDIVPSVGRTGVITPTANLRPVEVSGVTVSRATLHNWEEMERKDIRIGDTVVIERAGDVIPAVVKVLTEKRSG
SERPLPIPAVCPECGSEVVKIPDEVAVRCMGLSCPAQIRESIIHFASRDAMDMEGLGEKYIEQLLRLGLVKNVADLYTLT
KDQFMQFDRMGDKLAENLLNAIEASKQRELSRFIFALGIRHVGEHTAKLLAGAFGSIENLERATEEELLSIREVGPQVAR
SIITFFRNEGNRETIRRMFKAGVRPTAEEKKVGGRFTGKTFVFTGALTRFSRPEAQKMVEKEGGHAAGSVSKKTDYVVAG
AEAGSKLDKARQLGVRVLTEDEFLAMLEEG

Sequences:

>Translated_670_residues
MDKQTAADRVAALRTELERHNRLYYAEDRPEITDAEYDLLFRELVDLETRFPDLAAPDSPTQRVGGAPLDKFEQVTHRIP
MLSLENAFTDVEIADFDDRVKRFLGLHGDVEIDYVCEPKMDGLAVELVYERGILTVGSTRGDGVVGENVTHNLKTVRGIP
LRLRCEQPPELLEVRGEVYLPLAAFQRLNAQREEEGEPPFANPRNAAAGSIRQLDSRITARRPLAIFCYAPGEVRGASFA
AQTEFLDRIGEWGLPVNPLIRPVKGVAEILAYYREMTERRDSLPYEIDGVVVKVDSFALQRELGEKSRSPRWAVAVKFPP
RQAVTVVEDIVPSVGRTGVITPTANLRPVEVSGVTVSRATLHNWEEMERKDIRIGDTVVIERAGDVIPAVVKVLTEKRSG
SERPLPIPAVCPECGSEVVKIPDEVAVRCMGLSCPAQIRESIIHFASRDAMDMEGLGEKYIEQLLRLGLVKNVADLYTLT
KDQFMQFDRMGDKLAENLLNAIEASKQRELSRFIFALGIRHVGEHTAKLLAGAFGSIENLERATEEELLSIREVGPQVAR
SIITFFRNEGNRETIRRMFKAGVRPTAEEKKVGGRFTGKTFVFTGALTRFSRPEAQKMVEKEGGHAAGSVSKKTDYVVAG
AEAGSKLDKARQLGVRVLTEDEFLAMLEEG
>Mature_670_residues
MDKQTAADRVAALRTELERHNRLYYAEDRPEITDAEYDLLFRELVDLETRFPDLAAPDSPTQRVGGAPLDKFEQVTHRIP
MLSLENAFTDVEIADFDDRVKRFLGLHGDVEIDYVCEPKMDGLAVELVYERGILTVGSTRGDGVVGENVTHNLKTVRGIP
LRLRCEQPPELLEVRGEVYLPLAAFQRLNAQREEEGEPPFANPRNAAAGSIRQLDSRITARRPLAIFCYAPGEVRGASFA
AQTEFLDRIGEWGLPVNPLIRPVKGVAEILAYYREMTERRDSLPYEIDGVVVKVDSFALQRELGEKSRSPRWAVAVKFPP
RQAVTVVEDIVPSVGRTGVITPTANLRPVEVSGVTVSRATLHNWEEMERKDIRIGDTVVIERAGDVIPAVVKVLTEKRSG
SERPLPIPAVCPECGSEVVKIPDEVAVRCMGLSCPAQIRESIIHFASRDAMDMEGLGEKYIEQLLRLGLVKNVADLYTLT
KDQFMQFDRMGDKLAENLLNAIEASKQRELSRFIFALGIRHVGEHTAKLLAGAFGSIENLERATEEELLSIREVGPQVAR
SIITFFRNEGNRETIRRMFKAGVRPTAEEKKVGGRFTGKTFVFTGALTRFSRPEAQKMVEKEGGHAAGSVSKKTDYVVAG
AEAGSKLDKARQLGVRVLTEDEFLAMLEEG

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=661, Percent_Identity=48.714069591528, Blast_Score=612, Evalue=1e-176,
Organism=Escherichia coli, GI87082305, Length=574, Percent_Identity=24.5644599303136, Blast_Score=106, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_GEOSL (Q74ER9)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_951947.1
- HSSP:   O87703
- ProteinModelPortal:   Q74ER9
- GeneID:   2687161
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU0890
- NMPDR:   fig|243231.1.peg.885
- TIGR:   GSU0890
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK828084
- BioCyc:   GSUL243231:GSU_0890-MONOMER
- BRENDA:   6.5.1.2
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 74577; Mature: 74577

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 119-119 BINDING 117-117 BINDING 140-140 BINDING 177-177 BINDING 293-293 BINDING 317-317

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKQTAADRVAALRTELERHNRLYYAEDRPEITDAEYDLLFRELVDLETRFPDLAAPDSP
CCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
TQRVGGAPLDKFEQVTHRIPMLSLENAFTDVEIADFDDRVKRFLGLHGDVEIDYVCEPKM
HHHCCCCCHHHHHHHHHHCCCEECCCCCCCCEECCHHHHHHHHHCCCCCEEEEEEECCCC
DGLAVELVYERGILTVGSTRGDGVVGENVTHNLKTVRGIPLRLRCEQPPELLEVRGEVYL
CCEEEEEEECCCEEEECCCCCCCCCCCCCHHHHHHHCCCCEEEECCCCHHHHHHCCCEEE
PLAAFQRLNAQREEEGEPPFANPRNAAAGSIRQLDSRITARRPLAIFCYAPGEVRGASFA
HHHHHHHHCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHH
AQTEFLDRIGEWGLPVNPLIRPVKGVAEILAYYREMTERRDSLPYEIDGVVVKVDSFALQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEEEHHHHHH
RELGEKSRSPRWAVAVKFPPRQAVTVVEDIVPSVGRTGVITPTANLRPVEVSGVTVSRAT
HHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEECCCCCCCEEEECCEEEEHHH
LHNWEEMERKDIRIGDTVVIERAGDVIPAVVKVLTEKRSGSERPLPIPAVCPECGSEVVK
HHCHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCHHHEE
IPDEVAVRCMGLSCPAQIRESIIHFASRDAMDMEGLGEKYIEQLLRLGLVKNVADLYTLT
CCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHH
KDQFMQFDRMGDKLAENLLNAIEASKQRELSRFIFALGIRHVGEHTAKLLAGAFGSIENL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
ERATEEELLSIREVGPQVARSIITFFRNEGNRETIRRMFKAGVRPTAEEKKVGGRFTGKT
HHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHCCCEECCCE
FVFTGALTRFSRPEAQKMVEKEGGHAAGSVSKKTDYVVAGAEAGSKLDKARQLGVRVLTE
EEEEHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHCCCEEECC
DEFLAMLEEG
HHHHHHHCCC
>Mature Secondary Structure
MDKQTAADRVAALRTELERHNRLYYAEDRPEITDAEYDLLFRELVDLETRFPDLAAPDSP
CCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
TQRVGGAPLDKFEQVTHRIPMLSLENAFTDVEIADFDDRVKRFLGLHGDVEIDYVCEPKM
HHHCCCCCHHHHHHHHHHCCCEECCCCCCCCEECCHHHHHHHHHCCCCCEEEEEEECCCC
DGLAVELVYERGILTVGSTRGDGVVGENVTHNLKTVRGIPLRLRCEQPPELLEVRGEVYL
CCEEEEEEECCCEEEECCCCCCCCCCCCCHHHHHHHCCCCEEEECCCCHHHHHHCCCEEE
PLAAFQRLNAQREEEGEPPFANPRNAAAGSIRQLDSRITARRPLAIFCYAPGEVRGASFA
HHHHHHHHCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHH
AQTEFLDRIGEWGLPVNPLIRPVKGVAEILAYYREMTERRDSLPYEIDGVVVKVDSFALQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEEEHHHHHH
RELGEKSRSPRWAVAVKFPPRQAVTVVEDIVPSVGRTGVITPTANLRPVEVSGVTVSRAT
HHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEECCCCCCCEEEECCEEEEHHH
LHNWEEMERKDIRIGDTVVIERAGDVIPAVVKVLTEKRSGSERPLPIPAVCPECGSEVVK
HHCHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCHHHEE
IPDEVAVRCMGLSCPAQIRESIIHFASRDAMDMEGLGEKYIEQLLRLGLVKNVADLYTLT
CCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHH
KDQFMQFDRMGDKLAENLLNAIEASKQRELSRFIFALGIRHVGEHTAKLLAGAFGSIENL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
ERATEEELLSIREVGPQVARSIITFFRNEGNRETIRRMFKAGVRPTAEEKKVGGRFTGKT
HHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHCCCEECCCE
FVFTGALTRFSRPEAQKMVEKEGGHAAGSVSKKTDYVVAGAEAGSKLDKARQLGVRVLTE
EEEEHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHCCCEEECC
DEFLAMLEEG
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA