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The map label for this gene is dsbC [H]

Identifier: 39995956

GI number: 39995956

Start: 910016

End: 910822

Strand: Direct

Name: dsbC [H]

Synonym: GSU0850

Alternate gene names: 39995956

Gene position: 910016-910822 (Clockwise)

Preceding gene: 39995955

Following gene: 39995957

Centisome position: 23.86

GC content: 59.85

Gene sequence:

>807_bases
ATGACGAAACGACTGATCAACTCCTGTCTGGTCGGGGCGCTGGTTGCCGTACTGGCAGCAGGCTCAGCCCTCGCTTCCCC
GAAGGAGGGGGCCAAAGAGGAGAGCCCCGAAGCGACGATCAAGGCGCTCTTCCCAACGCTGCCTCTGACAAGCGTTAAGA
AAACCCCAATCGACGGCCTCTTCGAGGTGATTACCGACGGCAACGTCATCTACATCTATCCGAAGACCGGCAACGTCATC
ATCGGCAATCTCTTCACCAAGGAGGGCAAGAACCTGACGGCAGAGGCGAGCAACCGCCTGGTAGCGGATCGCTACAAGCT
CATCACCGCCGCCGACAAGGAGAAAGCGGTGAAAGTCGGCAACGGCAAGTACGAGGTCATCGAGATCACCGACCCCGATT
GCCCCTATTGCCGCAAGATGCACGAGTACTGGGGCCGCAGGCCCGACGTGACCCGTTACGTCTTCTTCCTTCCCCTTGCC
ATGCATCCCGATGCCGAGAAGAAGATCCGCTACATCCTGTCCGCCGAGAACAAGGAACTGGCCTTCTGGGAAGTCTATTC
GGGCGAGTTGGACAACAAGCGTGAGATCCTTGACGCCCCCCGGGACGACAAGGGGCTTCTCGCCGCCCACAAGGCGATCG
TGGCCAAGCTGGGCGTGAACTCTACTCCCACCTTCTGGGTGCAGGGAACCTATGTGAACGGCGCCAATACCCAGCTCATC
GAGAAGGTGATCGGCACGTGCAAGACCGCCGAGGGCATGGCTCCCGGTGCTCCTGTCAAGTGCGAGGACGAGGGGCAGAA
GAAATAG

Upstream 100 bases:

>100_bases
TTTAGTCGCGACGGGGGTTTTAGGATATGTAACTTGATGAAACCTATATTAAATGGTATCACTGAACCCATTAAGCGAAA
CGTTTTACCACGGAGGTATC

Downstream 100 bases:

>100_bases
CGGAGGCCTCGCACTTCCGTGATCCCGCTCAAGGACTACAACCCGACGCGACGCTTGCCGGTGGTGAGCGTCGCGCTCAT
CATTCTCAATTGTGCCGCGT

Product: hypothetical protein

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MTKRLINSCLVGALVAVLAAGSALASPKEGAKEESPEATIKALFPTLPLTSVKKTPIDGLFEVITDGNVIYIYPKTGNVI
IGNLFTKEGKNLTAEASNRLVADRYKLITAADKEKAVKVGNGKYEVIEITDPDCPYCRKMHEYWGRRPDVTRYVFFLPLA
MHPDAEKKIRYILSAENKELAFWEVYSGELDNKREILDAPRDDKGLLAAHKAIVAKLGVNSTPTFWVQGTYVNGANTQLI
EKVIGTCKTAEGMAPGAPVKCEDEGQKK

Sequences:

>Translated_268_residues
MTKRLINSCLVGALVAVLAAGSALASPKEGAKEESPEATIKALFPTLPLTSVKKTPIDGLFEVITDGNVIYIYPKTGNVI
IGNLFTKEGKNLTAEASNRLVADRYKLITAADKEKAVKVGNGKYEVIEITDPDCPYCRKMHEYWGRRPDVTRYVFFLPLA
MHPDAEKKIRYILSAENKELAFWEVYSGELDNKREILDAPRDDKGLLAAHKAIVAKLGVNSTPTFWVQGTYVNGANTQLI
EKVIGTCKTAEGMAPGAPVKCEDEGQKK
>Mature_267_residues
TKRLINSCLVGALVAVLAAGSALASPKEGAKEESPEATIKALFPTLPLTSVKKTPIDGLFEVITDGNVIYIYPKTGNVII
GNLFTKEGKNLTAEASNRLVADRYKLITAADKEKAVKVGNGKYEVIEITDPDCPYCRKMHEYWGRRPDVTRYVFFLPLAM
HPDAEKKIRYILSAENKELAFWEVYSGELDNKREILDAPRDDKGLLAAHKAIVAKLGVNSTPTFWVQGTYVNGANTQLIE
KVIGTCKTAEGMAPGAPVKCEDEGQKK

Specific function: Required for disulfide bond formation in some periplasmic proteins. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbC is reoxidized by a yet uncharacterized protein. Also acts as a disulfide isomerase [H]

COG id: COG1651

COG function: function code O; Protein-disulfide isomerase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1789260, Length=227, Percent_Identity=27.7533039647577, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018950
- InterPro:   IPR009094
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF10411 DsbC_N [H]

EC number: NA

Molecular weight: Translated: 29375; Mature: 29244

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRLINSCLVGALVAVLAAGSALASPKEGAKEESPEATIKALFPTLPLTSVKKTPIDGL
CCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHH
FEVITDGNVIYIYPKTGNVIIGNLFTKEGKNLTAEASNRLVADRYKLITAADKEKAVKVG
HHHHCCCCEEEEEECCCCEEEEEEEECCCCCEEHHHHCCEEHHHHHEEEECCCCCEEEEC
NGKYEVIEITDPDCPYCRKMHEYWGRRPDVTRYVFFLPLAMHPDAEKKIRYILSAENKEL
CCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEEEEEECCCCHHHHHEEEEECCCCCE
AFWEVYSGELDNKREILDAPRDDKGLLAAHKAIVAKLGVNSTPTFWVQGTYVNGANTQLI
EEEEEECCCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCHHHH
EKVIGTCKTAEGMAPGAPVKCEDEGQKK
HHHHHHHCCCCCCCCCCCEEECCCCCCC
>Mature Secondary Structure 
TKRLINSCLVGALVAVLAAGSALASPKEGAKEESPEATIKALFPTLPLTSVKKTPIDGL
CHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHH
FEVITDGNVIYIYPKTGNVIIGNLFTKEGKNLTAEASNRLVADRYKLITAADKEKAVKVG
HHHHCCCCEEEEEECCCCEEEEEEEECCCCCEEHHHHCCEEHHHHHEEEECCCCCEEEEC
NGKYEVIEITDPDCPYCRKMHEYWGRRPDVTRYVFFLPLAMHPDAEKKIRYILSAENKEL
CCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEEEEEECCCCHHHHHEEEEECCCCCE
AFWEVYSGELDNKREILDAPRDDKGLLAAHKAIVAKLGVNSTPTFWVQGTYVNGANTQLI
EEEEEECCCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCHHHH
EKVIGTCKTAEGMAPGAPVKCEDEGQKK
HHHHHHHCCCCCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]