Click here to switch to the map view.

The map label for this gene is glnG [H]

Identifier: 39995895

GI number: 39995895

Start: 848545

End: 848922

Strand: Reverse

Name: glnG [H]

Synonym: GSU0789

Alternate gene names: 39995895

Gene position: 848922-848545 (Counterclockwise)

Preceding gene: 39995896

Following gene: 39995882

Centisome position: 22.26

GC content: 63.49

Gene sequence:

>378_bases
ATGTTCGGTGGTTCCGTGCTGATCTGTGACGATGAAGAAGGGATGCTGCGTTACCTCAAAAAGATGATCGAGGCGGCGGG
GCTGAGGGTTGAAACCTTCGGCAACGGAACGAGCCTGCTGGCGAGGATCGAGGGGGGAGACCCCGGAGACGCGAGTCTGC
TGTTGCAGGACATGCGGCTCCCTGATGCCGACGGGTTGGAGATTCTGCATCGGGTGAAGGAACTGCGCCCTGCCCTCCCC
GTGGTGATGATGACCGCCTACGCCTGCGACGAGGTGGTCCGCGCGGCCTTTGACGGCGGGGCCAGCGATTTCCTGCCCAA
GCCCTTCACCCGCGAGGCGGTGCTCGGCGTTATCAGGACGACGACGGCGGAACACTAG

Upstream 100 bases:

>100_bases
ATTGAGCTTAACCACAGTCGAACCATTGGTTTCCTGAACGGGGTGCGACATTTTGTCCCAATACAGAGCAGAAATGATCA
CACAAGTCATGGAGGACGTC

Downstream 100 bases:

>100_bases
CACAACGGGCAGGACCATAACAAAAGAGAGGAGCCTCGCGGCCCCTCTCTTTTGTTATGACATCAACGGGCGGACGACGT
CGCATGCGGCAGGTTGCCGT

Product: response regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 125; Mature: 125

Protein sequence:

>125_residues
MFGGSVLICDDEEGMLRYLKKMIEAAGLRVETFGNGTSLLARIEGGDPGDASLLLQDMRLPDADGLEILHRVKELRPALP
VVMMTAYACDEVVRAAFDGGASDFLPKPFTREAVLGVIRTTTAEH

Sequences:

>Translated_125_residues
MFGGSVLICDDEEGMLRYLKKMIEAAGLRVETFGNGTSLLARIEGGDPGDASLLLQDMRLPDADGLEILHRVKELRPALP
VVMMTAYACDEVVRAAFDGGASDFLPKPFTREAVLGVIRTTTAEH
>Mature_125_residues
MFGGSVLICDDEEGMLRYLKKMIEAAGLRVETFGNGTSLLARIEGGDPGDASLLLQDMRLPDADGLEILHRVKELRPALP
VVMMTAYACDEVVRAAFDGGASDFLPKPFTREAVLGVIRTTTAEH

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=109, Percent_Identity=37.6146788990826, Blast_Score=80, Evalue=4e-17,
Organism=Escherichia coli, GI1790860, Length=117, Percent_Identity=33.3333333333333, Blast_Score=61, Evalue=2e-11,
Organism=Escherichia coli, GI1786911, Length=108, Percent_Identity=36.1111111111111, Blast_Score=60, Evalue=3e-11,
Organism=Escherichia coli, GI1788191, Length=107, Percent_Identity=34.5794392523364, Blast_Score=59, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 13524; Mature: 13524

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGGSVLICDDEEGMLRYLKKMIEAAGLRVETFGNGTSLLARIEGGDPGDASLLLQDMRL
CCCCCEEEEECCHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHCC
PDADGLEILHRVKELRPALPVVMMTAYACDEVVRAAFDGGASDFLPKPFTREAVLGVIRT
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
TTAEH
HCCCC
>Mature Secondary Structure
MFGGSVLICDDEEGMLRYLKKMIEAAGLRVETFGNGTSLLARIEGGDPGDASLLLQDMRL
CCCCCEEEEECCHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHCC
PDADGLEILHRVKELRPALPVVMMTAYACDEVVRAAFDGGASDFLPKPFTREAVLGVIRT
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
TTAEH
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]