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The map label for this gene is hybC [H]

Identifier: 39995891

GI number: 39995891

Start: 845690

End: 847372

Strand: Direct

Name: hybC [H]

Synonym: GSU0785

Alternate gene names: 39995891

Gene position: 845690-847372 (Clockwise)

Preceding gene: 39995890

Following gene: 39995892

Centisome position: 22.17

GC content: 63.7

Gene sequence:

>1683_bases
ATGTCTAAACGTATCACCATAGACCCCATCACCCGGATCGAGGGTCACCTGAGAATCGATGTGGAAGTGAATGGCGGCCA
GGTTGCCAAGGCCTGGTCCTCGGCCCAGATGTGGCGGGGCATCGAGACCATCCTCAAGGGGCGCGATCCCCAGGACGCCT
GGTCCTACGCCCAGCGTTTCTGCGGCGTCTGCACTACGGTGCACGCCATCTCGTCCATCCGTTCCGTGGAGAACGCCCTG
AACGTGGAAGTTCCCCTCAACGCCCAGTACATCCGCAATATCATGATCGCCCAGCACTCGGTGCAGGATCACATCGTCCA
CTTCTACCACCTGTCCGCCCTGGACTGGGTCGACATCGTGTCCGCCCTGAAGGCCGACCCGAAGAAGGCCTCCTCCATCG
CCCAGAGCCTGTCCGACTGGCCCGGCAACAGCGAGAAGGAGTTCAAGGCGGTCCAGGACAAGCTCAAGGCGTTCGTGGCC
AGCGGCCAGCTCGGCATCTTCGCCTCCGGCTACTGGGGCCATCCGGCCATGAAGCTGCCGCCCGAGGTGAACCTGATCGC
CGTGGCCCACTACCTCAAGGCTCTCGACTACCAGCGCCGGGCTTCACAGGCTGTGGCCATTCTCGGCGGCAAGAACCCCC
ACGTTCAGAACCTGGTGGTGGGCGGGGTAGCCACCGCTGTGAACATGGAGAACATCGCCACCCTCAACATGGAGCGGATC
GCCTTCCTCCGCACCCTTATGGAAGAGACCCGCGAGTTCGTCCAGAAGGTCTACTACCCGGACCTGGTGGCCATCGCTTC
CTTCTACAAGGAATGGTTCAAGTACGGCGCCGGCGTCACCAACTACCTGGCGGTCCCCGAGTTCGCCGAAGACACCCGCA
ACACCAAGTTCGGCCTCCCCGGCGGCACCATCTACGGCGGCGATCTGGGTACCTTCAAGGCGATCACCACCCACCAGGAT
GCGGCGCTCATTCAGGGGGTCACCGAAGGGGTGGCTCACGCCTGGTACGAAGGCTCCGATTCGCTCCACCCCTGGGAAGG
GGAGACCAAGCCCCAGTACACCGACTTCCAGGAGAACGGCAAGTACACCTGGTGCAAGTCGCCGCGCTACAACGGCAAGC
CCATGCAGGTTGGACCGCCCGCCCAGGTCATGGCCGCCTACGCCACCGGCCATCCCAAGGTCAAGAAGCTGGTGGACGAC
GCCGCAGCCAAGCTCGGCATCGGTCCCAAGGAGCTCCACTCCACCATGGGCCGGCTCTTCTGCCGGGGCGTGCGCGCCCA
CGTCATGGCCGACTACTCCCTGGAGTACCTGGACAAGCTCGTGGCCAACATCGGTAAGGGTGACTCCACCTACGCCAACC
ACACGGAAATCCCCGACGGCGAGTACAAGGGGGTCGGCTTCCACGAGGCGCCCCGTGGTGCCCTGTCCCACTGGATAGTC
ATCGAGAAGAAGAAGATCAAGAACTACCAGGCCGTGGTTCCGTCCACCTGGAACGCCTCGCCGCGGGACGAGAACGGCGT
TGCCGGTCCCTACGAAGCCTGCCTCGTGGGCAACCCCGTGGCTCAGCCGGACAAGCCGCTGGAAGTGCTGCGCACCATCC
ACTCCTTCGACCCCTGCATTGCGTGCGCCGTCCACACCATCGACCCGGCCGGCAAGGAGATCACCAAGGTCAAGGTCCTG
TAA

Upstream 100 bases:

>100_bases
TACGCAGGAAAATAATGGGCGGTGGCCGGCGCCCGGGGAGCATCCCCGGCCGCCGGCCGACCCGCGGTCGAACGCATACA
ATTCATCTCTGGAGGAATAC

Downstream 100 bases:

>100_bases
CGGAAACCCGGGGGGGGCTTCAGCCCCCCTCTATGGAGTAGCGAATGAGAACCCTAATCTTCGGCGCCGGCAACCTGATC
CTCACCGACGAGGGCTTCGG

Product: nickel-dependent hydrogenase large subunit

Products: NA

Alternate protein names: HYD2; Membrane-bound hydrogenase 2 large subunit; NiFe hydrogenase [H]

Number of amino acids: Translated: 560; Mature: 559

Protein sequence:

>560_residues
MSKRITIDPITRIEGHLRIDVEVNGGQVAKAWSSAQMWRGIETILKGRDPQDAWSYAQRFCGVCTTVHAISSIRSVENAL
NVEVPLNAQYIRNIMIAQHSVQDHIVHFYHLSALDWVDIVSALKADPKKASSIAQSLSDWPGNSEKEFKAVQDKLKAFVA
SGQLGIFASGYWGHPAMKLPPEVNLIAVAHYLKALDYQRRASQAVAILGGKNPHVQNLVVGGVATAVNMENIATLNMERI
AFLRTLMEETREFVQKVYYPDLVAIASFYKEWFKYGAGVTNYLAVPEFAEDTRNTKFGLPGGTIYGGDLGTFKAITTHQD
AALIQGVTEGVAHAWYEGSDSLHPWEGETKPQYTDFQENGKYTWCKSPRYNGKPMQVGPPAQVMAAYATGHPKVKKLVDD
AAAKLGIGPKELHSTMGRLFCRGVRAHVMADYSLEYLDKLVANIGKGDSTYANHTEIPDGEYKGVGFHEAPRGALSHWIV
IEKKKIKNYQAVVPSTWNASPRDENGVAGPYEACLVGNPVAQPDKPLEVLRTIHSFDPCIACAVHTIDPAGKEITKVKVL

Sequences:

>Translated_560_residues
MSKRITIDPITRIEGHLRIDVEVNGGQVAKAWSSAQMWRGIETILKGRDPQDAWSYAQRFCGVCTTVHAISSIRSVENAL
NVEVPLNAQYIRNIMIAQHSVQDHIVHFYHLSALDWVDIVSALKADPKKASSIAQSLSDWPGNSEKEFKAVQDKLKAFVA
SGQLGIFASGYWGHPAMKLPPEVNLIAVAHYLKALDYQRRASQAVAILGGKNPHVQNLVVGGVATAVNMENIATLNMERI
AFLRTLMEETREFVQKVYYPDLVAIASFYKEWFKYGAGVTNYLAVPEFAEDTRNTKFGLPGGTIYGGDLGTFKAITTHQD
AALIQGVTEGVAHAWYEGSDSLHPWEGETKPQYTDFQENGKYTWCKSPRYNGKPMQVGPPAQVMAAYATGHPKVKKLVDD
AAAKLGIGPKELHSTMGRLFCRGVRAHVMADYSLEYLDKLVANIGKGDSTYANHTEIPDGEYKGVGFHEAPRGALSHWIV
IEKKKIKNYQAVVPSTWNASPRDENGVAGPYEACLVGNPVAQPDKPLEVLRTIHSFDPCIACAVHTIDPAGKEITKVKVL
>Mature_559_residues
SKRITIDPITRIEGHLRIDVEVNGGQVAKAWSSAQMWRGIETILKGRDPQDAWSYAQRFCGVCTTVHAISSIRSVENALN
VEVPLNAQYIRNIMIAQHSVQDHIVHFYHLSALDWVDIVSALKADPKKASSIAQSLSDWPGNSEKEFKAVQDKLKAFVAS
GQLGIFASGYWGHPAMKLPPEVNLIAVAHYLKALDYQRRASQAVAILGGKNPHVQNLVVGGVATAVNMENIATLNMERIA
FLRTLMEETREFVQKVYYPDLVAIASFYKEWFKYGAGVTNYLAVPEFAEDTRNTKFGLPGGTIYGGDLGTFKAITTHQDA
ALIQGVTEGVAHAWYEGSDSLHPWEGETKPQYTDFQENGKYTWCKSPRYNGKPMQVGPPAQVMAAYATGHPKVKKLVDDA
AAKLGIGPKELHSTMGRLFCRGVRAHVMADYSLEYLDKLVANIGKGDSTYANHTEIPDGEYKGVGFHEAPRGALSHWIVI
EKKKIKNYQAVVPSTWNASPRDENGVAGPYEACLVGNPVAQPDKPLEVLRTIHSFDPCIACAVHTIDPAGKEITKVKVL

Specific function: This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=567, Percent_Identity=55.5555555555556, Blast_Score=668, Evalue=0.0,
Organism=Escherichia coli, GI1787207, Length=585, Percent_Identity=43.5897435897436, Blast_Score=507, Evalue=1e-145,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 61654; Mature: 61523

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRITIDPITRIEGHLRIDVEVNGGQVAKAWSSAQMWRGIETILKGRDPQDAWSYAQRF
CCCCEECCCHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
CGVCTTVHAISSIRSVENALNVEVPLNAQYIRNIMIAQHSVQDHIVHFYHLSALDWVDIV
HHHHHHHHHHHHHHHHHHHHCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SALKADPKKASSIAQSLSDWPGNSEKEFKAVQDKLKAFVASGQLGIFASGYWGHPAMKLP
HHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
PEVNLIAVAHYLKALDYQRRASQAVAILGGKNPHVQNLVVGGVATAVNMENIATLNMERI
CCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHEEEHHHHHHCCHHHHHHCCHHHH
AFLRTLMEETREFVQKVYYPDLVAIASFYKEWFKYGAGVTNYLAVPEFAEDTRNTKFGLP
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHCCCCCCCCC
GGTIYGGDLGTFKAITTHQDAALIQGVTEGVAHAWYEGSDSLHPWEGETKPQYTDFQENG
CCCEECCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCC
KYTWCKSPRYNGKPMQVGPPAQVMAAYATGHPKVKKLVDDAAAKLGIGPKELHSTMGRLF
CEEEECCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
CRGVRAHVMADYSLEYLDKLVANIGKGDSTYANHTEIPDGEYKGVGFHEAPRGALSHWIV
HHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEEE
IEKKKIKNYQAVVPSTWNASPRDENGVAGPYEACLVGNPVAQPDKPLEVLRTIHSFDPCI
EEHHHHCCCEEECCCCCCCCCCCCCCCCCCHHHHEECCCCCCCCCHHHHHHHHHCCCHHH
ACAVHTIDPAGKEITKVKVL
HHHHHCCCCCCCCCEEEECC
>Mature Secondary Structure 
SKRITIDPITRIEGHLRIDVEVNGGQVAKAWSSAQMWRGIETILKGRDPQDAWSYAQRF
CCCEECCCHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
CGVCTTVHAISSIRSVENALNVEVPLNAQYIRNIMIAQHSVQDHIVHFYHLSALDWVDIV
HHHHHHHHHHHHHHHHHHHHCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SALKADPKKASSIAQSLSDWPGNSEKEFKAVQDKLKAFVASGQLGIFASGYWGHPAMKLP
HHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
PEVNLIAVAHYLKALDYQRRASQAVAILGGKNPHVQNLVVGGVATAVNMENIATLNMERI
CCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHEEEHHHHHHCCHHHHHHCCHHHH
AFLRTLMEETREFVQKVYYPDLVAIASFYKEWFKYGAGVTNYLAVPEFAEDTRNTKFGLP
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHCCCCCCCCC
GGTIYGGDLGTFKAITTHQDAALIQGVTEGVAHAWYEGSDSLHPWEGETKPQYTDFQENG
CCCEECCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCC
KYTWCKSPRYNGKPMQVGPPAQVMAAYATGHPKVKKLVDDAAAKLGIGPKELHSTMGRLF
CEEEECCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
CRGVRAHVMADYSLEYLDKLVANIGKGDSTYANHTEIPDGEYKGVGFHEAPRGALSHWIV
HHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEEE
IEKKKIKNYQAVVPSTWNASPRDENGVAGPYEACLVGNPVAQPDKPLEVLRTIHSFDPCI
EEHHHHCCCEEECCCCCCCCCCCCCCCCCCHHHHEECCCCCCCCCHHHHHHHHHCCCHHH
ACAVHTIDPAGKEITKVKVL
HHHHHCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]