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The map label for this gene is hybA [H]

Identifier: 39995889

GI number: 39995889

Start: 843357

End: 844274

Strand: Direct

Name: hybA [H]

Synonym: GSU0783

Alternate gene names: 39995889

Gene position: 843357-844274 (Clockwise)

Preceding gene: 39995888

Following gene: 39995890

Centisome position: 22.11

GC content: 63.51

Gene sequence:

>918_bases
ATGAAAAGCACGACTAGACGCGATTTCCTCAAGATGATGGGGATGACCGGCGCCGCCTGCCTGGCGTGCGCCGCGCCCCT
GTCGGCCTCCACGGGCCCGGCCGGCGAGAGCGAAACCGCCATCGCCATGCTTTACGACGCCACCAAGTGCGTGGGGTGCA
AGGCGTGCATGGCAGCCTGCAAGCGGGTCAACATGGAGCAGAACAATCTCTCCTACGAGTACCTGCCCACCGACGGGGAC
AAACTCTGGGACGCCCCCAAGGACCTGTCCGGCGACACCCGCACGGTCATCAAGCTCTACAAGGAGTCCGACACCCGCTT
CTCCTACGTGAAGCACTCCTGTATGCACTGCACGAAGCCCGGCTGCGTGTCCGCCTGCCCGGTCAAGGCCATGACCAAGG
ACCCGGTCACCGGCGTGGTGGCCTACAACAAGAACGCCTGCATCGGCTGCCGCTACTGCCAGGTGGCCTGTCCCTACAAC
ATCCCCCGCTTCCAGTGGGACAAGGCGCTGCCCCAGATCGTCAAGTGCGATTTCTGCAAGGACACGAACCTGAAGGCCAA
GGGGATGCCCGCCTGCAGCGAGACCTGCCCCGCCGGCGCCATTGCCTTCGGCAAGCGCACGGATCTTCTGGCCGAGGCCC
ACAACCGGATCAAGGAGAACCCGGACCGCTACATTCCAAAGGTCTACGGCGAGAAGGAAGTTGGCGGCGCCAACCACCTG
TATCTGGCCGCCTTCCCGTTCCAGAAGCTGGGCCTGCCCGAACTGAAGGAGGAGTCGCCCGCCGCCTTCTCCGAGCATAT
CCAGCACACCATCTACAAGGGGTTCATCGCCCCGGTTGCCCTCTACGGGACCCTGGCGGCCATTGCCCTCAGGAACAAGA
AGAAGCAGGAAGATCTCGGACACGGGGAGGACGATTAA

Upstream 100 bases:

>100_bases
GTGAGCCCGGCGGCCACCGGTCTCGTTGGCCTGGCCGGCGGCGTAGCCCTGGGTGCCGGCGCCATGCTGGCCAAGAAGCT
GCCCAGCGGGGAGGAAGGTC

Downstream 100 bases:

>100_bases
GATGGGATCGCATCACGATGAATATCAGGTTCAGCCCGGCAAGATCTTAACCCGGCCCTTCTTCATCCTCCTGTTCTTCG
TCCTGGTAGGGCTGGCGCTC

Product: hydrogenase 2 protein HybA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MKSTTRRDFLKMMGMTGAACLACAAPLSASTGPAGESETAIAMLYDATKCVGCKACMAACKRVNMEQNNLSYEYLPTDGD
KLWDAPKDLSGDTRTVIKLYKESDTRFSYVKHSCMHCTKPGCVSACPVKAMTKDPVTGVVAYNKNACIGCRYCQVACPYN
IPRFQWDKALPQIVKCDFCKDTNLKAKGMPACSETCPAGAIAFGKRTDLLAEAHNRIKENPDRYIPKVYGEKEVGGANHL
YLAAFPFQKLGLPELKEESPAAFSEHIQHTIYKGFIAPVALYGTLAAIALRNKKKQEDLGHGEDD

Sequences:

>Translated_305_residues
MKSTTRRDFLKMMGMTGAACLACAAPLSASTGPAGESETAIAMLYDATKCVGCKACMAACKRVNMEQNNLSYEYLPTDGD
KLWDAPKDLSGDTRTVIKLYKESDTRFSYVKHSCMHCTKPGCVSACPVKAMTKDPVTGVVAYNKNACIGCRYCQVACPYN
IPRFQWDKALPQIVKCDFCKDTNLKAKGMPACSETCPAGAIAFGKRTDLLAEAHNRIKENPDRYIPKVYGEKEVGGANHL
YLAAFPFQKLGLPELKEESPAAFSEHIQHTIYKGFIAPVALYGTLAAIALRNKKKQEDLGHGEDD
>Mature_305_residues
MKSTTRRDFLKMMGMTGAACLACAAPLSASTGPAGESETAIAMLYDATKCVGCKACMAACKRVNMEQNNLSYEYLPTDGD
KLWDAPKDLSGDTRTVIKLYKESDTRFSYVKHSCMHCTKPGCVSACPVKAMTKDPVTGVVAYNKNACIGCRYCQVACPYN
IPRFQWDKALPQIVKCDFCKDTNLKAKGMPACSETCPAGAIAFGKRTDLLAEAHNRIKENPDRYIPKVYGEKEVGGANHL
YLAAFPFQKLGLPELKEESPAAFSEHIQHTIYKGFIAPVALYGTLAAIALRNKKKQEDLGHGEDD

Specific function: Participates in the periplasmic electron-transferring activity of hydrogenase 2 during its catalytic turnover [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789370, Length=338, Percent_Identity=36.9822485207101, Blast_Score=227, Evalue=9e-61,
Organism=Escherichia coli, GI1787749, Length=254, Percent_Identity=33.8582677165354, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1790326, Length=264, Percent_Identity=32.5757575757576, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI2367345, Length=225, Percent_Identity=31.5555555555556, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI226510944, Length=208, Percent_Identity=31.25, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1787872, Length=171, Percent_Identity=32.1637426900585, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787122, Length=179, Percent_Identity=32.4022346368715, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87082180, Length=156, Percent_Identity=30.7692307692308, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87082179, Length=188, Percent_Identity=29.2553191489362, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787478, Length=106, Percent_Identity=33.9622641509434, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI2367245, Length=179, Percent_Identity=25.6983240223464, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 33338; Mature: 33338

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
9.2 %Cys+Met (Translated Protein)
5.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
9.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSTTRRDFLKMMGMTGAACLACAAPLSASTGPAGESETAIAMLYDATKCVGCKACMAAC
CCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHH
KRVNMEQNNLSYEYLPTDGDKLWDAPKDLSGDTRTVIKLYKESDTRFSYVKHSCMHCTKP
HHCCCCCCCCEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCC
GCVSACPVKAMTKDPVTGVVAYNKNACIGCRYCQVACPYNIPRFQWDKALPQIVKCDFCK
CCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCC
DTNLKAKGMPACSETCPAGAIAFGKRTDLLAEAHNRIKENPDRYIPKVYGEKEVGGANHL
CCCCCCCCCCCCHHCCCCCCEECCCHHHHHHHHHHHHHCCCHHHCHHHCCCCCCCCCCEE
YLAAFPFQKLGLPELKEESPAAFSEHIQHTIYKGFIAPVALYGTLAAIALRNKKKQEDLG
EEEECCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
HGEDD
CCCCC
>Mature Secondary Structure
MKSTTRRDFLKMMGMTGAACLACAAPLSASTGPAGESETAIAMLYDATKCVGCKACMAAC
CCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHH
KRVNMEQNNLSYEYLPTDGDKLWDAPKDLSGDTRTVIKLYKESDTRFSYVKHSCMHCTKP
HHCCCCCCCCEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCC
GCVSACPVKAMTKDPVTGVVAYNKNACIGCRYCQVACPYNIPRFQWDKALPQIVKCDFCK
CCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCC
DTNLKAKGMPACSETCPAGAIAFGKRTDLLAEAHNRIKENPDRYIPKVYGEKEVGGANHL
CCCCCCCCCCCCHHCCCCCCEECCCHHHHHHHHHHHHHCCCHHHCHHHCCCCCCCCCCEE
YLAAFPFQKLGLPELKEESPAAFSEHIQHTIYKGFIAPVALYGTLAAIALRNKKKQEDLG
EEEECCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
HGEDD
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]