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The map label for this gene is hyfB [H]

Identifier: 39995840

GI number: 39995840

Start: 780533

End: 782539

Strand: Reverse

Name: hyfB [H]

Synonym: GSU0734

Alternate gene names: 39995840

Gene position: 782539-780533 (Counterclockwise)

Preceding gene: 39995841

Following gene: 39995838

Centisome position: 20.52

GC content: 69.56

Gene sequence:

>2007_bases
ATGACTGATGGAGGGGGAATGGTCTCGCCCGGTATGCTCCTGCTCTGCGCCTCGGCCCTTGCCGCCCTGTCGGGGGTGCC
GCTGCTCGTCAGAAGCCTTTCCCCTGCCGCCGGCCAGAAGGCGGCCACAGTGCTCATGCTCCTGGCCACCGTGATCGGCC
TGGGCAGCGCCGTCGCCACCCTCGTCACCGGCCGGACCGAAACCTTTCTCCTCTCCTGGGGACTCCCCTTCGGTCCCGCC
GAAATGGCCGTGGACCCCCTCTCCGCCTTTTTTGCCCTGCCGATCCTCCTGGTGGCCGCCTGCTGCGCGGTCTATGCCCT
CGGCTACTGGCCGGCCCGGGACAATCCCCGCACGGTGCGCAGCCTGACCTTCTTCTTCGGCCTCCTCGTAGCAGCCCTGA
TCGGGGTGATCGTAGCGCGCAACGGGGTCCTCTTCCTCGTGGCGTGGGAGATCATGGCCGTTTCCGCCTGGTTCGTCCTC
ACCGCCGACCACGCGAAACCCGAGGTCCGGGATGCCGGCACCCTCTACCTGATCACCACCCACATCGGCACCCTGGCCCT
GTTCGCCCTGTTTTCGCTCCTCAAGGGGACGGCGGGATCGTACCTCTTCCCGGCCCCGGCATCGCTGGCGGCCGGCGGAG
GGATCGGCGCCGGCATCTTCCTGACCGCACTCCTGGGCTTCGGCATCAAGGCTGGGCTCATGCCGCTCCACGTCTGGCTC
CCGTCGGCCCACGCCAACGCCCCGAGCCACATCTCGGCCATCATGTCCGGGGTAATCCTGAAGCTCGGCATCTACGGCAT
CGTCCGGACGGTTTCCTTCTTTGCCCAGATTCCGCTCTGGTGGGGGATCGTCCTGCTGGTGGCGGGAATGGCGTCGGGGA
TCGCCGGGGTCGCCTTCGCTCTGGGTCAGCATGACCTCAAGCGGTTGCTGGCCTACCACAGCATCGAGAACATCGGGATC
ATCGCCATGGGGATCGGCGTGGCCCTGGTGGGCGCGGCCACGGGGGTTCCCTCCCTGGCACTCCTCGGCATGGCAGGGGC
ACTGCTCCACGTACTGAACCACGCCACCTTCAAGGCGCTCCTTTTCCTCGGCGCCGGCTCGGTGATCCATGCCGTCGGCA
CCCGGAAAATCGACCGGATGGGGGGCATCGCCCGCGTCATGCCCCGGACCGCCCTTCTCTTCCTGACCGGGGCCGTGGCC
ATCTGCGGGCTGCCGCCCCTGAACGGCTTCGTGAGCGAGTTCCTCATCTACCTGGGGGTCTTCCGGGAGGCGGTCCACGG
CACCGGCGCGGCCGCGGCCGTCACATCACTGGCCGGGCCGGCCCTTGCCCTGGTGGGGGGACTGGCGGTTGCCTGCTTCG
TCAAGGTCTTCGGCGTGGTTTTCCTGGGGCTCCCCCGGAGCGAAGAGGCGGCCCGCGCCCACGAGGCGGGGCGCCCCATG
CTCGCGCCCATGGCCGTGCTGGGCGCGGTCTGCGTCGTGGTCGGCCTGGCACCGGCGGCGGTGGCGCCGCTGCTTGACGC
CGCGGTGCGCTCCTGGCGGCCGGAACTGGCTCTCACGGCCCCGGCCCTGGCCGATCTGGCGCCCCTGGGATGGCTCATGG
CCGTTGCCGCCGGCACCCTTGCCACGGTGGCCGTTCTCGCTCTCCTCTACGCCCGGCGGTTGGCCGCCGTGCCCCGGGGA
GCCTCGCCCACCTGGGGCTGCGGCTATCTGGCCCCCACCCCCCGGATGCAGTACACCGCGTCATCCTTCGCCGAGATGCT
GGTCCGGCTCTTCGGCGGCATCCTGCGCCCCCACGGCACCCAGCCCGGCATCCGCTCCTGTGCGCCGGCGCCGTCGGAGC
TCCATACCCATGTCCCCGAGACGGTGCTCGAACACCTCTACCTCCCGTTCATCACGTGGGCCAATGGTAAGCTCGCCCCG
CTCCGGAGGCTCCAGCACGGCCAGTTGCACCTCTACATCCTCTACACCCTGATCACTCTGGTCACGCTCCTGCTGTTCGG
CATGTGA

Upstream 100 bases:

>100_bases
CGCGACCCGCGGCTGCGGGAGGCCCTGAAACGACCCTGCGACCCGGCGGGCATCCTGGCCGCCGTGGCCGAAATCGAACG
ATCTCTGGGAGGATGAGCCG

Downstream 100 bases:

>100_bases
CCGCCCGGCTACCCCCGTTCGACCCGCCGGAGCACCCCCAGGTAGTCGAGCACCGCGGCCGAGCCTCGGGCCACGCAGGA
AAAGGGATCGCTCACCCGCG

Product: NAD-dependent dehydrogenase subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 668; Mature: 667

Protein sequence:

>668_residues
MTDGGGMVSPGMLLLCASALAALSGVPLLVRSLSPAAGQKAATVLMLLATVIGLGSAVATLVTGRTETFLLSWGLPFGPA
EMAVDPLSAFFALPILLVAACCAVYALGYWPARDNPRTVRSLTFFFGLLVAALIGVIVARNGVLFLVAWEIMAVSAWFVL
TADHAKPEVRDAGTLYLITTHIGTLALFALFSLLKGTAGSYLFPAPASLAAGGGIGAGIFLTALLGFGIKAGLMPLHVWL
PSAHANAPSHISAIMSGVILKLGIYGIVRTVSFFAQIPLWWGIVLLVAGMASGIAGVAFALGQHDLKRLLAYHSIENIGI
IAMGIGVALVGAATGVPSLALLGMAGALLHVLNHATFKALLFLGAGSVIHAVGTRKIDRMGGIARVMPRTALLFLTGAVA
ICGLPPLNGFVSEFLIYLGVFREAVHGTGAAAAVTSLAGPALALVGGLAVACFVKVFGVVFLGLPRSEEAARAHEAGRPM
LAPMAVLGAVCVVVGLAPAAVAPLLDAAVRSWRPELALTAPALADLAPLGWLMAVAAGTLATVAVLALLYARRLAAVPRG
ASPTWGCGYLAPTPRMQYTASSFAEMLVRLFGGILRPHGTQPGIRSCAPAPSELHTHVPETVLEHLYLPFITWANGKLAP
LRRLQHGQLHLYILYTLITLVTLLLFGM

Sequences:

>Translated_668_residues
MTDGGGMVSPGMLLLCASALAALSGVPLLVRSLSPAAGQKAATVLMLLATVIGLGSAVATLVTGRTETFLLSWGLPFGPA
EMAVDPLSAFFALPILLVAACCAVYALGYWPARDNPRTVRSLTFFFGLLVAALIGVIVARNGVLFLVAWEIMAVSAWFVL
TADHAKPEVRDAGTLYLITTHIGTLALFALFSLLKGTAGSYLFPAPASLAAGGGIGAGIFLTALLGFGIKAGLMPLHVWL
PSAHANAPSHISAIMSGVILKLGIYGIVRTVSFFAQIPLWWGIVLLVAGMASGIAGVAFALGQHDLKRLLAYHSIENIGI
IAMGIGVALVGAATGVPSLALLGMAGALLHVLNHATFKALLFLGAGSVIHAVGTRKIDRMGGIARVMPRTALLFLTGAVA
ICGLPPLNGFVSEFLIYLGVFREAVHGTGAAAAVTSLAGPALALVGGLAVACFVKVFGVVFLGLPRSEEAARAHEAGRPM
LAPMAVLGAVCVVVGLAPAAVAPLLDAAVRSWRPELALTAPALADLAPLGWLMAVAAGTLATVAVLALLYARRLAAVPRG
ASPTWGCGYLAPTPRMQYTASSFAEMLVRLFGGILRPHGTQPGIRSCAPAPSELHTHVPETVLEHLYLPFITWANGKLAP
LRRLQHGQLHLYILYTLITLVTLLLFGM
>Mature_667_residues
TDGGGMVSPGMLLLCASALAALSGVPLLVRSLSPAAGQKAATVLMLLATVIGLGSAVATLVTGRTETFLLSWGLPFGPAE
MAVDPLSAFFALPILLVAACCAVYALGYWPARDNPRTVRSLTFFFGLLVAALIGVIVARNGVLFLVAWEIMAVSAWFVLT
ADHAKPEVRDAGTLYLITTHIGTLALFALFSLLKGTAGSYLFPAPASLAAGGGIGAGIFLTALLGFGIKAGLMPLHVWLP
SAHANAPSHISAIMSGVILKLGIYGIVRTVSFFAQIPLWWGIVLLVAGMASGIAGVAFALGQHDLKRLLAYHSIENIGII
AMGIGVALVGAATGVPSLALLGMAGALLHVLNHATFKALLFLGAGSVIHAVGTRKIDRMGGIARVMPRTALLFLTGAVAI
CGLPPLNGFVSEFLIYLGVFREAVHGTGAAAAVTSLAGPALALVGGLAVACFVKVFGVVFLGLPRSEEAARAHEAGRPML
APMAVLGAVCVVVGLAPAAVAPLLDAAVRSWRPELALTAPALADLAPLGWLMAVAAGTLATVAVLALLYARRLAAVPRGA
SPTWGCGYLAPTPRMQYTASSFAEMLVRLFGGILRPHGTQPGIRSCAPAPSELHTHVPETVLEHLYLPFITWANGKLAPL
RRLQHGQLHLYILYTLITLVTLLLFGM

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=389, Percent_Identity=23.3933161953728, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI251831116, Length=296, Percent_Identity=26.3513513513513, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1788827, Length=609, Percent_Identity=35.632183908046, Blast_Score=332, Evalue=7e-92,
Organism=Escherichia coli, GI2367154, Length=516, Percent_Identity=35.8527131782946, Blast_Score=243, Evalue=4e-65,
Organism=Escherichia coli, GI1788831, Length=354, Percent_Identity=32.4858757062147, Blast_Score=152, Evalue=9e-38,
Organism=Escherichia coli, GI1788829, Length=427, Percent_Identity=27.1662763466042, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1788613, Length=328, Percent_Identity=29.2682926829268, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1788614, Length=313, Percent_Identity=31.3099041533546, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 69052; Mature: 68921

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDGGGMVSPGMLLLCASALAALSGVPLLVRSLSPAAGQKAATVLMLLATVIGLGSAVAT
CCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LVTGRTETFLLSWGLPFGPAEMAVDPLSAFFALPILLVAACCAVYALGYWPARDNPRTVR
HHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
SLTFFFGLLVAALIGVIVARNGVLFLVAWEIMAVSAWFVLTADHAKPEVRDAGTLYLITT
HHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHEEEECCCCCCCCCCCEEEEHHH
HIGTLALFALFSLLKGTAGSYLFPAPASLAAGGGIGAGIFLTALLGFGIKAGLMPLHVWL
HHHHHHHHHHHHHHHCCCCCEECCCCHHHHCCCCCHHHHHHHHHHHCCHHHCCEEEEEEC
PSAHANAPSHISAIMSGVILKLGIYGIVRTVSFFAQIPLWWGIVLLVAGMASGIAGVAFA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGQHDLKRLLAYHSIENIGIIAMGIGVALVGAATGVPSLALLGMAGALLHVLNHATFKAL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LFLGAGSVIHAVGTRKIDRMGGIARVMPRTALLFLTGAVAICGLPPLNGFVSEFLIYLGV
HHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
FREAVHGTGAAAAVTSLAGPALALVGGLAVACFVKVFGVVFLGLPRSEEAARAHEAGRPM
HHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCH
LAPMAVLGAVCVVVGLAPAAVAPLLDAAVRSWRPELALTAPALADLAPLGWLMAVAAGTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHH
ATVAVLALLYARRLAAVPRGASPTWGCGYLAPTPRMQYTASSFAEMLVRLFGGILRPHGT
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
QPGIRSCAPAPSELHTHVPETVLEHLYLPFITWANGKLAPLRRLQHGQLHLYILYTLITL
CCCHHHCCCCCHHHHHCCHHHHHHHHHHHHEEECCCCCHHHHHHCCCCCHHHHHHHHHHH
VTLLLFGM
HHHHHHCC
>Mature Secondary Structure 
TDGGGMVSPGMLLLCASALAALSGVPLLVRSLSPAAGQKAATVLMLLATVIGLGSAVAT
CCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LVTGRTETFLLSWGLPFGPAEMAVDPLSAFFALPILLVAACCAVYALGYWPARDNPRTVR
HHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
SLTFFFGLLVAALIGVIVARNGVLFLVAWEIMAVSAWFVLTADHAKPEVRDAGTLYLITT
HHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHEEEECCCCCCCCCCCEEEEHHH
HIGTLALFALFSLLKGTAGSYLFPAPASLAAGGGIGAGIFLTALLGFGIKAGLMPLHVWL
HHHHHHHHHHHHHHHCCCCCEECCCCHHHHCCCCCHHHHHHHHHHHCCHHHCCEEEEEEC
PSAHANAPSHISAIMSGVILKLGIYGIVRTVSFFAQIPLWWGIVLLVAGMASGIAGVAFA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGQHDLKRLLAYHSIENIGIIAMGIGVALVGAATGVPSLALLGMAGALLHVLNHATFKAL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LFLGAGSVIHAVGTRKIDRMGGIARVMPRTALLFLTGAVAICGLPPLNGFVSEFLIYLGV
HHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
FREAVHGTGAAAAVTSLAGPALALVGGLAVACFVKVFGVVFLGLPRSEEAARAHEAGRPM
HHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCH
LAPMAVLGAVCVVVGLAPAAVAPLLDAAVRSWRPELALTAPALADLAPLGWLMAVAAGTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHH
ATVAVLALLYARRLAAVPRGASPTWGCGYLAPTPRMQYTASSFAEMLVRLFGGILRPHGT
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
QPGIRSCAPAPSELHTHVPETVLEHLYLPFITWANGKLAPLRRLQHGQLHLYILYTLITL
CCCHHHCCCCCHHHHHCCHHHHHHHHHHHHEEECCCCCHHHHHHCCCCCHHHHHHHHHHH
VTLLLFGM
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2016588 [H]