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The map label for this gene is ptsN [H]

Identifier: 39995841

GI number: 39995841

Start: 782544

End: 783221

Strand: Reverse

Name: ptsN [H]

Synonym: GSU0735

Alternate gene names: 39995841

Gene position: 783221-782544 (Counterclockwise)

Preceding gene: 39995842

Following gene: 39995840

Centisome position: 20.53

GC content: 68.88

Gene sequence:

>678_bases
ATGTTGTTGAACGCCGCGGAAGCGGCCCGGCTACTTTCAACCGACGAAAAGACCGTCAAGCGCTGGATCAGGCGGGATGG
GCTCCCGGCCACGCCGGTGGACGGCGAGCCGCGGATCAACCGCATCGACCTGCTGGAGTGGGCCACGGAGCGGGGGATCA
AGGTCCTGCCCGAGGCCTATGCCGGCGCCGGAGACGAGCCGGGGCTCTTCCCGTCCCTCTCGTCGGCCCTGCAGGCGGGG
GGCATCCACTGCAACGTGCCGGGGGACGACAAGCTGACCGTCCTGCGCAATGTGGTGGACCTCCTCCCCCTCCCGCAGCA
GGTGGACCCCGAATTCGTACTCCAGGTGCTCCTGGCCCGGGAAGCCCTGGGCACCACCGCCATCGGCGACGGCATCGCCA
TCCCCCACATGCGCAACCCGATCCTGCTCCACGTGGCCGGATCGAGCATCACCCTCTGCCACTTGGCTACGCCCGTGGAC
TTCGGCGCCCTGGACGGCAAGCCGGTGCAGATCCTCTTCACCCTCACCAGTCCCACCGTGAAGGCCCACCTTCACCTGCT
GTCGAGGCTGGCCCATGCCCTGCGCGACCCGCGGCTGCGGGAGGCCCTGAAACGACCCTGCGACCCGGCGGGCATCCTGG
CCGCCGTGGCCGAAATCGAACGATCTCTGGGAGGATGA

Upstream 100 bases:

>100_bases
GAGGGCGGGCTGGTGACGCGTGGAGATGTTCGGAGCGGGAGCCCCGCCCTCCGGAGGACCGGCGCCGAACTAGCGAACTG
AACGCCCACGAGGTGAATCC

Downstream 100 bases:

>100_bases
GCCGATGACTGATGGAGGGGGAATGGTCTCGCCCGGTATGCTCCTGCTCTGCGCCTCGGCCCTTGCCGCCCTGTCGGGGG
TGCCGCTGCTCGTCAGAAGC

Product: PTS system fructose subfamily IIA subunit

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MLLNAAEAARLLSTDEKTVKRWIRRDGLPATPVDGEPRINRIDLLEWATERGIKVLPEAYAGAGDEPGLFPSLSSALQAG
GIHCNVPGDDKLTVLRNVVDLLPLPQQVDPEFVLQVLLAREALGTTAIGDGIAIPHMRNPILLHVAGSSITLCHLATPVD
FGALDGKPVQILFTLTSPTVKAHLHLLSRLAHALRDPRLREALKRPCDPAGILAAVAEIERSLGG

Sequences:

>Translated_225_residues
MLLNAAEAARLLSTDEKTVKRWIRRDGLPATPVDGEPRINRIDLLEWATERGIKVLPEAYAGAGDEPGLFPSLSSALQAG
GIHCNVPGDDKLTVLRNVVDLLPLPQQVDPEFVLQVLLAREALGTTAIGDGIAIPHMRNPILLHVAGSSITLCHLATPVD
FGALDGKPVQILFTLTSPTVKAHLHLLSRLAHALRDPRLREALKRPCDPAGILAAVAEIERSLGG
>Mature_225_residues
MLLNAAEAARLLSTDEKTVKRWIRRDGLPATPVDGEPRINRIDLLEWATERGIKVLPEAYAGAGDEPGLFPSLSSALQAG
GIHCNVPGDDKLTVLRNVVDLLPLPQQVDPEFVLQVLLAREALGTTAIGDGIAIPHMRNPILLHVAGSSITLCHLATPVD
FGALDGKPVQILFTLTSPTVKAHLHLLSRLAHALRDPRLREALKRPCDPAGILAAVAEIERSLGG

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=138, Percent_Identity=36.231884057971, Blast_Score=68, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 24094; Mature: 24094

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLNAAEAARLLSTDEKTVKRWIRRDGLPATPVDGEPRINRIDLLEWATERGIKVLPEAY
CCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCEECCHHH
AGAGDEPGLFPSLSSALQAGGIHCNVPGDDKLTVLRNVVDLLPLPQQVDPEFVLQVLLAR
CCCCCCCCCCHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
EALGTTAIGDGIAIPHMRNPILLHVAGSSITLCHLATPVDFGALDGKPVQILFTLTSPTV
HHHCCCCCCCCEECCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCHH
KAHLHLLSRLAHALRDPRLREALKRPCDPAGILAAVAEIERSLGG
HHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLLNAAEAARLLSTDEKTVKRWIRRDGLPATPVDGEPRINRIDLLEWATERGIKVLPEAY
CCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCEECCHHH
AGAGDEPGLFPSLSSALQAGGIHCNVPGDDKLTVLRNVVDLLPLPQQVDPEFVLQVLLAR
CCCCCCCCCCHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
EALGTTAIGDGIAIPHMRNPILLHVAGSSITLCHLATPVDFGALDGKPVQILFTLTSPTV
HHHCCCCCCCCEECCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCHH
KAHLHLLSRLAHALRDPRLREALKRPCDPAGILAAVAEIERSLGG
HHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]