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The map label for this gene is barA [H]

Identifier: 39995824

GI number: 39995824

Start: 767717

End: 769486

Strand: Direct

Name: barA [H]

Synonym: GSU0718

Alternate gene names: 39995824

Gene position: 767717-769486 (Clockwise)

Preceding gene: 39995823

Following gene: 39995827

Centisome position: 20.13

GC content: 64.8

Gene sequence:

>1770_bases
ATGACCCAGTCCGGCCCAGAAGACGGCTCCGGCAATAGCCGGATCGGGGCAGCGGAAATGCCGGAAGCGGATACCGAGAA
GCAGCAGCCCGCGTCTGATGCTCCGAGTCAGGAGCTGCGGGATATCCGGGCGCAACTGGAGCTCTCCCTCGAACGTTACG
CCAACCTCTACCACTTCGCCCCCGTCGGCTATGCCACCATCGACGCCGTCGGGGTGATCGCCGACGCCAACCGGACCCTG
GCGGGGATGATCGGGGCCGATTGCCGGGAGATGGTGGGGCTCCTCTTCAGCGACTTTGTGCACAAGGATGACGTCACGTC
CTTCGTATCCCATCTGGCCCGCTGTACCGGACAGCGGGAGGGGGTCACCGGCGAGTTCCGCCTCAGCGGCAGGAACGGCA
TATCCGTGGACGTTCAGCTGTCGAGCATGCCTCTCACGCCCCCCGGCGGCCGGGTCTCGTGCCGCACGGCAATAACCGAC
ATCACCATGATTAAGCGCAAGGAGCGGGAGCTGAACGAGAGCCGCGAGGAACTGTGCTGGGCCAAGGAGGCGGCCGAGGA
TGCCAGCCGTGCCAAGAGCGAGTTCCTGGCCAACATGAGCCACGAGATCCGCACCCCCATCAACGGTGTGCTCGGCATGC
TCCAGCTGGTTCTCGACACGGAGCTGGGGGACGAGCAGCGTCACTGTCTGGAAATGGCCAAGCGCTCCGCCGACGCCCTC
CTGCGCCTGGTGAACGACATCCTCGACTTCTCCCGCATCGAGGCGCGGAAGATGGTCTTCGAAAGCCGTCCCTTCGACCT
GACGGCATGCGTTAACAGCGCCGTGGAAATCCTGGCCTTCGAGGCGCGGCGGAAGGGGCTTGCCGTCATGGTCCGCATGG
GGCCCGACGTCCCCGCGGCAGTCGTGGGGGATGAGGCCCGGTTGAGACAGGTGCTGGTGAACCTGATCGGGAACGCCGTC
AAGTTCACCGACAGAGGGAAGGTGGAGGTAACGGCGGCTCGGGCCGAGAGGCAGTCCGATCCCCTTCGCACCGCGATCCG
GATATCGGTACGCGACACCGGTATCGGCATTGCTCCGCAGGAGCGGGAGCGTCTTTTCAGGGTCTTCAGCCAGGTAGACG
GGGCCTGTAACCGCCGGTTCGGCGGCACGGGCCTCGGTCTGGCGCTGACCCGCGACATCGTGGAAGCCATGGGCGGGGCC
ATCGATGTCGAGAGCCAGTCGGGCCAGGGGAGCACCTTTACCATCACGATCCCGCTCCTGTTGGCAGTGACTGCGTCCTC
GCCCTTTCTCCCCGCCGATACGCTTCGCATAGCCGAGGCTTCGGCCGGAGGGGGCACCGGTGCGCCGGTCACCCCCCCAA
TTTCTATTCAATACGAGGAGGCACGGAAAGTGAAGATTCTTCTTGCCGAGGACGACCCCATTACCCGCAAGTTCATGGAG
ATCATGCTGGAGCGTCGCAATTTCGAATTCGAGTCCGCGAGCGACGGCCATGAAGCGGTTGAAAAATGGGAGGCGGGGGA
GTTCGACGTGGTGCTCATGGATGTGCAGATGCCCCGTCTCGACGGCTACGAGGCCACCGGCCTGATCCGCGAGAGGGAGC
GCGACCGGGGGCGGCGCACTCCCATCATCGCCCTTACCGCCCATGCCATGCGCAAGGACGAGGACCGGTGCCTCGACGCC
GGCATGGACGGCTATCTCTCAAAGCCCATCGATTTCACGAAGCTCATGGAGTGCATCGGCGAAATCCTGGCCCGGCGGGG
ACGGGGGTAA

Upstream 100 bases:

>100_bases
CCGGGAGCAGCCTACACACCCCTGAGCCATCCAGTGCACTGAAGCGCGCGGCTGTTTCCACTGACGGCCTCCAGCCAACG
CGCGCTGAGGAGAATTCTTC

Downstream 100 bases:

>100_bases
CACCGTCCCTCAGGCCCGGTCGCGTTCCCTCAGGATGGCCGACACCGCCCGCCAGAGCCGGAAGGCGTCCGTCAACTTCG
GCTTCAGCTCCACGTGCATG

Product: sensory box histidine kinase/response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 589; Mature: 588

Protein sequence:

>589_residues
MTQSGPEDGSGNSRIGAAEMPEADTEKQQPASDAPSQELRDIRAQLELSLERYANLYHFAPVGYATIDAVGVIADANRTL
AGMIGADCREMVGLLFSDFVHKDDVTSFVSHLARCTGQREGVTGEFRLSGRNGISVDVQLSSMPLTPPGGRVSCRTAITD
ITMIKRKERELNESREELCWAKEAAEDASRAKSEFLANMSHEIRTPINGVLGMLQLVLDTELGDEQRHCLEMAKRSADAL
LRLVNDILDFSRIEARKMVFESRPFDLTACVNSAVEILAFEARRKGLAVMVRMGPDVPAAVVGDEARLRQVLVNLIGNAV
KFTDRGKVEVTAARAERQSDPLRTAIRISVRDTGIGIAPQERERLFRVFSQVDGACNRRFGGTGLGLALTRDIVEAMGGA
IDVESQSGQGSTFTITIPLLLAVTASSPFLPADTLRIAEASAGGGTGAPVTPPISIQYEEARKVKILLAEDDPITRKFME
IMLERRNFEFESASDGHEAVEKWEAGEFDVVLMDVQMPRLDGYEATGLIRERERDRGRRTPIIALTAHAMRKDEDRCLDA
GMDGYLSKPIDFTKLMECIGEILARRGRG

Sequences:

>Translated_589_residues
MTQSGPEDGSGNSRIGAAEMPEADTEKQQPASDAPSQELRDIRAQLELSLERYANLYHFAPVGYATIDAVGVIADANRTL
AGMIGADCREMVGLLFSDFVHKDDVTSFVSHLARCTGQREGVTGEFRLSGRNGISVDVQLSSMPLTPPGGRVSCRTAITD
ITMIKRKERELNESREELCWAKEAAEDASRAKSEFLANMSHEIRTPINGVLGMLQLVLDTELGDEQRHCLEMAKRSADAL
LRLVNDILDFSRIEARKMVFESRPFDLTACVNSAVEILAFEARRKGLAVMVRMGPDVPAAVVGDEARLRQVLVNLIGNAV
KFTDRGKVEVTAARAERQSDPLRTAIRISVRDTGIGIAPQERERLFRVFSQVDGACNRRFGGTGLGLALTRDIVEAMGGA
IDVESQSGQGSTFTITIPLLLAVTASSPFLPADTLRIAEASAGGGTGAPVTPPISIQYEEARKVKILLAEDDPITRKFME
IMLERRNFEFESASDGHEAVEKWEAGEFDVVLMDVQMPRLDGYEATGLIRERERDRGRRTPIIALTAHAMRKDEDRCLDA
GMDGYLSKPIDFTKLMECIGEILARRGRG
>Mature_588_residues
TQSGPEDGSGNSRIGAAEMPEADTEKQQPASDAPSQELRDIRAQLELSLERYANLYHFAPVGYATIDAVGVIADANRTLA
GMIGADCREMVGLLFSDFVHKDDVTSFVSHLARCTGQREGVTGEFRLSGRNGISVDVQLSSMPLTPPGGRVSCRTAITDI
TMIKRKERELNESREELCWAKEAAEDASRAKSEFLANMSHEIRTPINGVLGMLQLVLDTELGDEQRHCLEMAKRSADALL
RLVNDILDFSRIEARKMVFESRPFDLTACVNSAVEILAFEARRKGLAVMVRMGPDVPAAVVGDEARLRQVLVNLIGNAVK
FTDRGKVEVTAARAERQSDPLRTAIRISVRDTGIGIAPQERERLFRVFSQVDGACNRRFGGTGLGLALTRDIVEAMGGAI
DVESQSGQGSTFTITIPLLLAVTASSPFLPADTLRIAEASAGGGTGAPVTPPISIQYEEARKVKILLAEDDPITRKFMEI
MLERRNFEFESASDGHEAVEKWEAGEFDVVLMDVQMPRLDGYEATGLIRERERDRGRRTPIIALTAHAMRKDEDRCLDAG
MDGYLSKPIDFTKLMECIGEILARRGRG

Specific function: Member of the two-component regulatory system UvrY/BarA involved in the regulation of carbon metabolism via the CsrA/CsrB regulatory system. Phosphorylates UvrY, probably via a four-step phosphorelay [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=243, Percent_Identity=46.9135802469136, Blast_Score=232, Evalue=5e-62,
Organism=Escherichia coli, GI48994928, Length=399, Percent_Identity=34.3358395989975, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1788713, Length=402, Percent_Identity=31.3432835820896, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI145693157, Length=250, Percent_Identity=41.2, Blast_Score=176, Evalue=5e-45,
Organism=Escherichia coli, GI87081816, Length=428, Percent_Identity=30.1401869158879, Blast_Score=175, Evalue=9e-45,
Organism=Escherichia coli, GI1790436, Length=231, Percent_Identity=29.004329004329, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1788393, Length=255, Percent_Identity=27.4509803921569, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1786600, Length=230, Percent_Identity=31.304347826087, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI1786783, Length=241, Percent_Identity=30.7053941908714, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI1790346, Length=239, Percent_Identity=30.5439330543933, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788549, Length=387, Percent_Identity=25.0645994832041, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1786912, Length=246, Percent_Identity=28.4552845528455, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082128, Length=260, Percent_Identity=28.4615384615385, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1788279, Length=233, Percent_Identity=26.6094420600858, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787894, Length=231, Percent_Identity=25.5411255411255, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790300, Length=256, Percent_Identity=27.734375, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI87082012, Length=113, Percent_Identity=29.2035398230088, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=150, Percent_Identity=38, Blast_Score=94, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6322000, Length=109, Percent_Identity=36.697247706422, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323034, Length=143, Percent_Identity=28.6713286713287, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR019247
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF09984 DUF2222; PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 64560; Mature: 64429

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSGPEDGSGNSRIGAAEMPEADTEKQQPASDAPSQELRDIRAQLELSLERYANLYHFA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHCC
PVGYATIDAVGVIADANRTLAGMIGADCREMVGLLFSDFVHKDDVTSFVSHLARCTGQRE
CCCHHHHHHHHEEECCCCHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
GVTGEFRLSGRNGISVDVQLSSMPLTPPGGRVSCRTAITDITMIKRKERELNESREELCW
CCCEEEEECCCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKEAAEDASRAKSEFLANMSHEIRTPINGVLGMLQLVLDTELGDEQRHCLEMAKRSADAL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LRLVNDILDFSRIEARKMVFESRPFDLTACVNSAVEILAFEARRKGLAVMVRMGPDVPAA
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHH
VVGDEARLRQVLVNLIGNAVKFTDRGKVEVTAARAERQSDPLRTAIRISVRDTGIGIAPQ
HCCCHHHHHHHHHHHHCCCEEECCCCCEEEEEHHHHCCCCCCEEEEEEEEEECCCCCCCH
ERERLFRVFSQVDGACNRRFGGTGLGLALTRDIVEAMGGAIDVESQSGQGSTFTITIPLL
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEHHHH
LAVTASSPFLPADTLRIAEASAGGGTGAPVTPPISIQYEEARKVKILLAEDDPITRKFME
HHHHCCCCCCCCHHHHEEECCCCCCCCCCCCCCCEEEECCCCEEEEEEECCCHHHHHHHH
IMLERRNFEFESASDGHEAVEKWEAGEFDVVLMDVQMPRLDGYEATGLIRERERDRGRRT
HHHHHHCCCCCCCCCHHHHHHHCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCC
PIIALTAHAMRKDEDRCLDAGMDGYLSKPIDFTKLMECIGEILARRGRG
CEEEEEHHHHHCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TQSGPEDGSGNSRIGAAEMPEADTEKQQPASDAPSQELRDIRAQLELSLERYANLYHFA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHCC
PVGYATIDAVGVIADANRTLAGMIGADCREMVGLLFSDFVHKDDVTSFVSHLARCTGQRE
CCCHHHHHHHHEEECCCCHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
GVTGEFRLSGRNGISVDVQLSSMPLTPPGGRVSCRTAITDITMIKRKERELNESREELCW
CCCEEEEECCCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKEAAEDASRAKSEFLANMSHEIRTPINGVLGMLQLVLDTELGDEQRHCLEMAKRSADAL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LRLVNDILDFSRIEARKMVFESRPFDLTACVNSAVEILAFEARRKGLAVMVRMGPDVPAA
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHH
VVGDEARLRQVLVNLIGNAVKFTDRGKVEVTAARAERQSDPLRTAIRISVRDTGIGIAPQ
HCCCHHHHHHHHHHHHCCCEEECCCCCEEEEEHHHHCCCCCCEEEEEEEEEECCCCCCCH
ERERLFRVFSQVDGACNRRFGGTGLGLALTRDIVEAMGGAIDVESQSGQGSTFTITIPLL
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEHHHH
LAVTASSPFLPADTLRIAEASAGGGTGAPVTPPISIQYEEARKVKILLAEDDPITRKFME
HHHHCCCCCCCCHHHHEEECCCCCCCCCCCCCCCEEEECCCCEEEEEEECCCHHHHHHHH
IMLERRNFEFESASDGHEAVEKWEAGEFDVVLMDVQMPRLDGYEATGLIRERERDRGRRT
HHHHHHCCCCCCCCCHHHHHHHCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCC
PIIALTAHAMRKDEDRCLDAGMDGYLSKPIDFTKLMECIGEILARRGRG
CEEEEEHHHHHCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]