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The map label for this gene is rpoH [H]

Identifier: 39995761

GI number: 39995761

Start: 690757

End: 691611

Strand: Reverse

Name: rpoH [H]

Synonym: GSU0655

Alternate gene names: 39995761

Gene position: 691611-690757 (Counterclockwise)

Preceding gene: 39995775

Following gene: 39995760

Centisome position: 18.13

GC content: 51.11

Gene sequence:

>855_bases
ATGTCGATGAGCTTACCTGTTGTAGCGGATAGCCTTACCGTCTACCTGAGAGAAATCAGAAAATTTCCCGTCCTCACTCC
TGAGGAGGAATACCGGTTTGCTGTTAAATTTTACGAGGACAAGGACCTGGAAGCGGCCCACTCTCTCATCACTTCGAATC
TTAGATTCGTTGTCAAGATCGCAGCCGAGTATCGTTCCTATGGACTGAAAATGCTCGATCTCATTCAGGAAGGCAACGTC
GGTCTGATGATGGCAGTACGTAAATTTAATCCCTACAAAGGGATACGTCTTATCTCCTATGCGGTCTGGTGGATCCGCGC
ATATATACAAAACCATATCATATCGGCATGGAGCCTCCTGAAGATAGGCACCACCCAGGCGCAGAAGAAGCTGTTCTTCA
AGCTGAATCAAGCAAAAAACGCGATCAAAGGGCTCACGGGCACCGACGACATCGAAGCAACAGCGACCTCGCTCCACGTG
AAAGAGTCCGAGGTCCTCGAAATGGACCAGCGGATGCGCGGTGACTTCTACCTCGACGCAGAAATCAAGAATGGCGACGG
ACTCACCATCCTGGAAACACTGGCTGACGAGCGGCAAAACCAAGAGGACACTCTGGCAGAACTCCAAGAGTCCGAGTTGC
TCAAGCAGCATATCGCCGGTGCTCTCGGCAAACTTAACGAGAAGGAGCGTTTTATCGTCGAGCAGCGCGTGACAGCCGAT
ACTCCGCTCACCCTTCAGGAGATCGCCGATCGATTCTCGATCTCCCGCGAACGTGTGCGCCAAATTGAAGAGGCTGCTCT
CCGTAAAATTAAATCATATCTTATCCCCATTATTGCAGAGACGCGACCGGTCTGA

Upstream 100 bases:

>100_bases
AATGGGCTACCTACCCGCTTGATTTTTAACACCCTACTTGTTACATTATTAGCACTCAGACGTACCGAGTGCTAATAACA
TCACGGTGGGAGGCGCGCAT

Downstream 100 bases:

>100_bases
CCATTTCGTCACACTCACGGCCCCACTCCTGAACGGGTGGGGCCTTTTTACATTACACCCGAGGGAACCGCATGCTTACA
AGTGATCAACAAGAGCGATA

Product: RNA polymerase sigma-32 factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MSMSLPVVADSLTVYLREIRKFPVLTPEEEYRFAVKFYEDKDLEAAHSLITSNLRFVVKIAAEYRSYGLKMLDLIQEGNV
GLMMAVRKFNPYKGIRLISYAVWWIRAYIQNHIISAWSLLKIGTTQAQKKLFFKLNQAKNAIKGLTGTDDIEATATSLHV
KESEVLEMDQRMRGDFYLDAEIKNGDGLTILETLADERQNQEDTLAELQESELLKQHIAGALGKLNEKERFIVEQRVTAD
TPLTLQEIADRFSISRERVRQIEEAALRKIKSYLIPIIAETRPV

Sequences:

>Translated_284_residues
MSMSLPVVADSLTVYLREIRKFPVLTPEEEYRFAVKFYEDKDLEAAHSLITSNLRFVVKIAAEYRSYGLKMLDLIQEGNV
GLMMAVRKFNPYKGIRLISYAVWWIRAYIQNHIISAWSLLKIGTTQAQKKLFFKLNQAKNAIKGLTGTDDIEATATSLHV
KESEVLEMDQRMRGDFYLDAEIKNGDGLTILETLADERQNQEDTLAELQESELLKQHIAGALGKLNEKERFIVEQRVTAD
TPLTLQEIADRFSISRERVRQIEEAALRKIKSYLIPIIAETRPV
>Mature_283_residues
SMSLPVVADSLTVYLREIRKFPVLTPEEEYRFAVKFYEDKDLEAAHSLITSNLRFVVKIAAEYRSYGLKMLDLIQEGNVG
LMMAVRKFNPYKGIRLISYAVWWIRAYIQNHIISAWSLLKIGTTQAQKKLFFKLNQAKNAIKGLTGTDDIEATATSLHVK
ESEVLEMDQRMRGDFYLDAEIKNGDGLTILETLADERQNQEDTLAELQESELLKQHIAGALGKLNEKERFIVEQRVTADT
PLTLQEIADRFSISRERVRQIEEAALRKIKSYLIPIIAETRPV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=276, Percent_Identity=39.4927536231884, Blast_Score=187, Evalue=5e-49,
Organism=Escherichia coli, GI1789098, Length=273, Percent_Identity=34.4322344322344, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1789448, Length=240, Percent_Identity=30, Blast_Score=98, Evalue=7e-22,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32570; Mature: 32439

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMSLPVVADSLTVYLREIRKFPVLTPEEEYRFAVKFYEDKDLEAAHSLITSNLRFVVKI
CCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHH
AAEYRSYGLKMLDLIQEGNVGLMMAVRKFNPYKGIRLISYAVWWIRAYIQNHIISAWSLL
HHHHHHHCHHHHHHHHCCCCEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIGTTQAQKKLFFKLNQAKNAIKGLTGTDDIEATATSLHVKESEVLEMDQRMRGDFYLDA
HHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHEECCHHHHHHHHHHHCCCEEEEE
EIKNGDGLTILETLADERQNQEDTLAELQESELLKQHIAGALGKLNEKERFIVEQRVTAD
EEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
TPLTLQEIADRFSISRERVRQIEEAALRKIKSYLIPIIAETRPV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SMSLPVVADSLTVYLREIRKFPVLTPEEEYRFAVKFYEDKDLEAAHSLITSNLRFVVKI
CCCCCHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHH
AAEYRSYGLKMLDLIQEGNVGLMMAVRKFNPYKGIRLISYAVWWIRAYIQNHIISAWSLL
HHHHHHHCHHHHHHHHCCCCEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIGTTQAQKKLFFKLNQAKNAIKGLTGTDDIEATATSLHVKESEVLEMDQRMRGDFYLDA
HHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHEECCHHHHHHHHHHHCCCEEEEE
EIKNGDGLTILETLADERQNQEDTLAELQESELLKQHIAGALGKLNEKERFIVEQRVTAD
EEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
TPLTLQEIADRFSISRERVRQIEEAALRKIKSYLIPIIAETRPV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1475207 [H]