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The map label for this gene is nadE [H]

Identifier: 39995758

GI number: 39995758

Start: 682713

End: 683534

Strand: Direct

Name: nadE [H]

Synonym: GSU0652

Alternate gene names: 39995758

Gene position: 682713-683534 (Clockwise)

Preceding gene: 39995757

Following gene: 39995759

Centisome position: 17.9

GC content: 58.03

Gene sequence:

>822_bases
ATGGGTGGGCTCATGGTTAATGCTGCGCTGCTGCGTCAAGTGTTGGTTGGCTTCCTGCGGGAAGAAGTCCACAAGGTGGG
CGTGCGCAAGGCGGTACTGGGATTATCGGGCGGCATCGACTCCGCTCTGGTCGCATTCCTTGCCGCCGAGGCACTCGGCC
CCGACAATGTGCACGCCATCATCATGCCCTACCGGACGAGCAATCCCGAGAGCGAAGCCCATGCCCGCCTTGTCGCCGAG
CAGCTGGGGATCAATCATGAGGTGATAGAAATAACGCCCATGGTAGATGCCTACTTCACCCGCTTCCCCGATGCGGACAA
CATGCGCCGCGGTAACAAAATGGCCCGTGAGCGGATGACAATCCTCTATGACCATTCCGCCGCTCTTGGGGCTCTTGTTC
TCGGTACCAGCAACAAGACCGAACTTCTGCTCGGCTATGGAACGCTTTACGGCGATATGGCGAGCGCTCTCAACCCGATC
GGCGACATATACAAAACACAGGTCTGGCAGCTCTCCGAGACGATGGGAGTTCCGCGGCAGATTATCGAAAAGAAGCCGTC
GGCCGATCTCTGGGCCGGCCAGACCGATGAGCAGGAGCTTGGCTTCACGTATCGTGAGGTTGATGAACTTCTCTACTGCA
TGATCGATCTCCGCATGAATCGTCTCGAGCTGCTTGCGCGCGGCTTTGTGGCAGATTTTATCGACACGGTGAGTGGCAAG
GTGCAGAATTCCCACTTCAAGCGGCGGCTGCCCGTCATCGCCAAGGTCTCCAACCGCACCATCGATCGCGATTTCCGCTA
TTCACGCGATTGGGGCAAGTGA

Upstream 100 bases:

>100_bases
CTTTGCGCCGCGAACGGATTTTTTCGCCGATGATGCGGGACGAGAGCCTCTTGATTACCCTGAGCGAATTGCGCCGAATC
GAGCGGGAGAGGGGACGCTG

Downstream 100 bases:

>100_bases
CGCGCATTCCGCGTCCGCAGCAGCGTTCCCGTGCCAGGCATCCTGTACATCATCGCGACTCCCATCGGCAACCTTGAGGA
TATCACCTACCGGGCAGTCC

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MGGLMVNAALLRQVLVGFLREEVHKVGVRKAVLGLSGGIDSALVAFLAAEALGPDNVHAIIMPYRTSNPESEAHARLVAE
QLGINHEVIEITPMVDAYFTRFPDADNMRRGNKMARERMTILYDHSAALGALVLGTSNKTELLLGYGTLYGDMASALNPI
GDIYKTQVWQLSETMGVPRQIIEKKPSADLWAGQTDEQELGFTYREVDELLYCMIDLRMNRLELLARGFVADFIDTVSGK
VQNSHFKRRLPVIAKVSNRTIDRDFRYSRDWGK

Sequences:

>Translated_273_residues
MGGLMVNAALLRQVLVGFLREEVHKVGVRKAVLGLSGGIDSALVAFLAAEALGPDNVHAIIMPYRTSNPESEAHARLVAE
QLGINHEVIEITPMVDAYFTRFPDADNMRRGNKMARERMTILYDHSAALGALVLGTSNKTELLLGYGTLYGDMASALNPI
GDIYKTQVWQLSETMGVPRQIIEKKPSADLWAGQTDEQELGFTYREVDELLYCMIDLRMNRLELLARGFVADFIDTVSGK
VQNSHFKRRLPVIAKVSNRTIDRDFRYSRDWGK
>Mature_272_residues
GGLMVNAALLRQVLVGFLREEVHKVGVRKAVLGLSGGIDSALVAFLAAEALGPDNVHAIIMPYRTSNPESEAHARLVAEQ
LGINHEVIEITPMVDAYFTRFPDADNMRRGNKMARERMTILYDHSAALGALVLGTSNKTELLLGYGTLYGDMASALNPIG
DIYKTQVWQLSETMGVPRQIIEKKPSADLWAGQTDEQELGFTYREVDELLYCMIDLRMNRLELLARGFVADFIDTVSGKV
QNSHFKRRLPVIAKVSNRTIDRDFRYSRDWGK

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=246, Percent_Identity=28.8617886178862, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788036, Length=214, Percent_Identity=28.5046728971963, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 30501; Mature: 30369

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGLMVNAALLRQVLVGFLREEVHKVGVRKAVLGLSGGIDSALVAFLAAEALGPDNVHAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEE
IMPYRTSNPESEAHARLVAEQLGINHEVIEITPMVDAYFTRFPDADNMRRGNKMARERMT
EEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ILYDHSAALGALVLGTSNKTELLLGYGTLYGDMASALNPIGDIYKTQVWQLSETMGVPRQ
HEEECCHHHHEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
IIEKKPSADLWAGQTDEQELGFTYREVDELLYCMIDLRMNRLELLARGFVADFIDTVSGK
HHCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
VQNSHFKRRLPVIAKVSNRTIDRDFRYSRDWGK
HHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GGLMVNAALLRQVLVGFLREEVHKVGVRKAVLGLSGGIDSALVAFLAAEALGPDNVHAI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEE
IMPYRTSNPESEAHARLVAEQLGINHEVIEITPMVDAYFTRFPDADNMRRGNKMARERMT
EEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ILYDHSAALGALVLGTSNKTELLLGYGTLYGDMASALNPIGDIYKTQVWQLSETMGVPRQ
HEEECCHHHHEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
IIEKKPSADLWAGQTDEQELGFTYREVDELLYCMIDLRMNRLELLARGFVADFIDTVSGK
HHCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
VQNSHFKRRLPVIAKVSNRTIDRDFRYSRDWGK
HHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA