Click here to switch to the map view.

The map label for this gene is nadE [H]

Identifier: 39995757

GI number: 39995757

Start: 681862

End: 682713

Strand: Direct

Name: nadE [H]

Synonym: GSU0651

Alternate gene names: 39995757

Gene position: 681862-682713 (Clockwise)

Preceding gene: 39995756

Following gene: 39995758

Centisome position: 17.88

GC content: 58.1

Gene sequence:

>852_bases
GTGGATTTCACTGTTGCACTGGCCCAGATCAAACCGAAACTTGGCTGTGTCGCCGATAACTGCCTCATGGTTCGTCAGGC
CGTGGAGCGGGGAATCGACGAGAAGGCCGATCTTGTCGTTTTTCCTGAACTGGCGCTTACGGGCTACTTTCTCAAAGACT
TGGTTCCCGATGTAGCACTGCGGCTGGACGCGCCTGAAATCAATGCCCTCCGGGAGCTTTCCCGCCATATTTCCATTGCG
GTGGGCCTTGTAGAGGTGTCGGCCGACTATCGTTTTTTCAATACCTCTCTCTATCTTGAAGGGGGAGAGGTGCGCCACGT
TCACAGAAAAGTGTACCTGCCCACCTACGGGCTCTTCGATGAGCAGCGTTATCTCGCGCGAGGCGAGCACTTCCGGGCCT
TCGACTCCCGCTTCGGCCGGATGGGGCTCCTCATATGCGAGGATATGTGGCATCTTTCCGCTCCCTACATTCTTGCCATG
GACGGTGCGACCACGGTGATCTGTCTCTCGAGCAGCCCTGGCCGGGGATTGACCGAGGATGACAGCCTCGGTTCGACCAT
TGCGTGGCAGAAGCTGACGTCGACGACGGCCATGTTTTTCAATTGCCGTGTTCTCTACTGCAACCGCGTAGGTTATGAAG
ACGGTGTCAATTTCTGGGGCGGCTCGGAGGTCGTGGCGCCATCGGGCGCAGTAACCAGCCGTGCGCGCATACTGGAGGAG
GATTTTCTGGTCGCCGGCGTAGACGAAGGCGCTTTGCGCCGCGAACGGATTTTTTCGCCGATGATGCGGGACGAGAGCCT
CTTGATTACCCTGAGCGAATTGCGCCGAATCGAGCGGGAGAGGGGACGCTGA

Upstream 100 bases:

>100_bases
ATATCACCAACGCTTTCGAGCTTGCCTACTGACCCGGAATTACCGTTGAAACCTCCCCACCCCTGTGGTACCGTAGGCCG
AAACTTACGGGGGAGATGTC

Downstream 100 bases:

>100_bases
TGGGTGGGCTCATGGTTAATGCTGCGCTGCTGCGTCAAGTGTTGGTTGGCTTCCTGCGGGAAGAAGTCCACAAGGTGGGC
GTGCGCAAGGCGGTACTGGG

Product: carbon-nitrogen family hydrolase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MDFTVALAQIKPKLGCVADNCLMVRQAVERGIDEKADLVVFPELALTGYFLKDLVPDVALRLDAPEINALRELSRHISIA
VGLVEVSADYRFFNTSLYLEGGEVRHVHRKVYLPTYGLFDEQRYLARGEHFRAFDSRFGRMGLLICEDMWHLSAPYILAM
DGATTVICLSSSPGRGLTEDDSLGSTIAWQKLTSTTAMFFNCRVLYCNRVGYEDGVNFWGGSEVVAPSGAVTSRARILEE
DFLVAGVDEGALRRERIFSPMMRDESLLITLSELRRIERERGR

Sequences:

>Translated_283_residues
MDFTVALAQIKPKLGCVADNCLMVRQAVERGIDEKADLVVFPELALTGYFLKDLVPDVALRLDAPEINALRELSRHISIA
VGLVEVSADYRFFNTSLYLEGGEVRHVHRKVYLPTYGLFDEQRYLARGEHFRAFDSRFGRMGLLICEDMWHLSAPYILAM
DGATTVICLSSSPGRGLTEDDSLGSTIAWQKLTSTTAMFFNCRVLYCNRVGYEDGVNFWGGSEVVAPSGAVTSRARILEE
DFLVAGVDEGALRRERIFSPMMRDESLLITLSELRRIERERGR
>Mature_283_residues
MDFTVALAQIKPKLGCVADNCLMVRQAVERGIDEKADLVVFPELALTGYFLKDLVPDVALRLDAPEINALRELSRHISIA
VGLVEVSADYRFFNTSLYLEGGEVRHVHRKVYLPTYGLFDEQRYLARGEHFRAFDSRFGRMGLLICEDMWHLSAPYILAM
DGATTVICLSSSPGRGLTEDDSLGSTIAWQKLTSTTAMFFNCRVLYCNRVGYEDGVNFWGGSEVVAPSGAVTSRARILEE
DFLVAGVDEGALRRERIFSPMMRDESLLITLSELRRIERERGR

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 31800; Mature: 31800

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFTVALAQIKPKLGCVADNCLMVRQAVERGIDEKADLVVFPELALTGYFLKDLVPDVAL
CCEEEEEEHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHE
RLDAPEINALRELSRHISIAVGLVEVSADYRFFNTSLYLEGGEVRHVHRKVYLPTYGLFD
EECCCHHHHHHHHHHHHEEEEEEEEEECCCEEEEEEEEEECCCEEEEEEEEECCCCCCCC
EQRYLARGEHFRAFDSRFGRMGLLICEDMWHLSAPYILAMDGATTVICLSSSPGRGLTED
CHHHHHCCCHHHHHHHHCCCCEEEEEHHHHHCCCCEEEEECCCEEEEEEECCCCCCCCCC
DSLGSTIAWQKLTSTTAMFFNCRVLYCNRVGYEDGVNFWGGSEVVAPSGAVTSRARILEE
CCCCCCHHHHHHHHHHEEEEEEEEEEEECCCCCCCCCCCCCCEEECCCCCHHHHHHHHHC
DFLVAGVDEGALRRERIFSPMMRDESLLITLSELRRIERERGR
CCEEEECCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHCC
>Mature Secondary Structure
MDFTVALAQIKPKLGCVADNCLMVRQAVERGIDEKADLVVFPELALTGYFLKDLVPDVAL
CCEEEEEEHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHE
RLDAPEINALRELSRHISIAVGLVEVSADYRFFNTSLYLEGGEVRHVHRKVYLPTYGLFD
EECCCHHHHHHHHHHHHEEEEEEEEEECCCEEEEEEEEEECCCEEEEEEEEECCCCCCCC
EQRYLARGEHFRAFDSRFGRMGLLICEDMWHLSAPYILAMDGATTVICLSSSPGRGLTED
CHHHHHCCCHHHHHHHHCCCCEEEEEHHHHHCCCCEEEEECCCEEEEEEECCCCCCCCCC
DSLGSTIAWQKLTSTTAMFFNCRVLYCNRVGYEDGVNFWGGSEVVAPSGAVTSRARILEE
CCCCCCHHHHHHHHHHEEEEEEEEEEEECCCCCCCCCCCCCCEEECCCCCHHHHHHHHHC
DFLVAGVDEGALRRERIFSPMMRDESLLITLSELRRIERERGR
CCEEEECCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]