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The map label for this gene is rnhB

Identifier: 39995755

GI number: 39995755

Start: 680763

End: 681416

Strand: Direct

Name: rnhB

Synonym: GSU0649

Alternate gene names: 39995755

Gene position: 680763-681416 (Clockwise)

Preceding gene: 39995754

Following gene: 39995756

Centisome position: 17.85

GC content: 62.08

Gene sequence:

>654_bases
GTGTCGTTAACCCTGTTCGATCATGAGCCGCAGCCGGCGGTCTGGGATTTCGAGAGCCGGGCTGCTCGCCGCGGCTATCG
CACCATTGCGGGCATTGATGAGGCAGGGCGCGGTCCCCTTGCCGGACCCGTAGTGGCGGCGGCCGTCATCCTTCCCTTCG
GCACCGACCTGCCGGGCGTCGACGATTCCAAAAAGCTCACCCCAGCACGACGAGATGACCTGTTTACCCTCATCCAAGGC
ACCGCCCTTGCTATCGGTGTCGGTGTGGCGGACCACAAGGTCATTGACCGGATCAATATCCTTCAGGCCACTCTCGCAGC
CATGCGCGAAGCCGTGGAATGTCTGACCGATCCGCCCGACTACCTTCTGGTGGACGGGATTTCCCCCGTCCCTCTTCCCG
TGGCTCAGCAAACCATCAAAAAAGGCGACAGTGCGAGCATTTCCATTGCCGCAGCTTCTATCATTGCCAAGGTCACCCGC
GACCGGCTGATGGCGGAGTACGATCAACTCTACCCCGGGTATGGCTTTGCCGAGCACAAGGGGTACGGGAGCGCCTCCCA
TATGGCAGCTATTGCCGCACTGGGGCCTTCGCCCATTCACCGCACAACCTTCCGCGGGGTCAGGGAGCACCTGGGAGAAA
GTCGGTGCCGGTAG

Upstream 100 bases:

>100_bases
CAAGGCCGCCCGCATCCGCGAAAAGAAGTACGTGGCGGCTCAGTAACCACGAAGCCCCGCCCAGTGCGGGGCTTTCGTTT
TTTCGGCTGGAGGGGAGGCA

Downstream 100 bases:

>100_bases
GAGAAGGCAGCCATCATAACAAGATCCTCGGTGTTCGCGGCGAGGACCTGGCCGCAGCCTACCTTGAACGGCTCCGTTAC
CGTATCATCGACCGGAACTT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MSLTLFDHEPQPAVWDFESRAARRGYRTIAGIDEAGRGPLAGPVVAAAVILPFGTDLPGVDDSKKLTPARRDDLFTLIQG
TALAIGVGVADHKVIDRINILQATLAAMREAVECLTDPPDYLLVDGISPVPLPVAQQTIKKGDSASISIAAASIIAKVTR
DRLMAEYDQLYPGYGFAEHKGYGSASHMAAIAALGPSPIHRTTFRGVREHLGESRCR

Sequences:

>Translated_217_residues
MSLTLFDHEPQPAVWDFESRAARRGYRTIAGIDEAGRGPLAGPVVAAAVILPFGTDLPGVDDSKKLTPARRDDLFTLIQG
TALAIGVGVADHKVIDRINILQATLAAMREAVECLTDPPDYLLVDGISPVPLPVAQQTIKKGDSASISIAAASIIAKVTR
DRLMAEYDQLYPGYGFAEHKGYGSASHMAAIAALGPSPIHRTTFRGVREHLGESRCR
>Mature_216_residues
SLTLFDHEPQPAVWDFESRAARRGYRTIAGIDEAGRGPLAGPVVAAAVILPFGTDLPGVDDSKKLTPARRDDLFTLIQGT
ALAIGVGVADHKVIDRINILQATLAAMREAVECLTDPPDYLLVDGISPVPLPVAQQTIKKGDSASISIAAASIIAKVTRD
RLMAEYDQLYPGYGFAEHKGYGSASHMAAIAALGPSPIHRTTFRGVREHLGESRCR

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=186, Percent_Identity=46.7741935483871, Blast_Score=155, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_GEOSL (Q74FG0)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_951706.1
- ProteinModelPortal:   Q74FG0
- GeneID:   2687304
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU0649
- NMPDR:   fig|243231.1.peg.644
- TIGR:   GSU0649
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- BioCyc:   GSUL243231:GSU_0649-MONOMER
- BRENDA:   3.1.26.4
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 23183; Mature: 23052

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTLFDHEPQPAVWDFESRAARRGYRTIAGIDEAGRGPLAGPVVAAAVILPFGTDLPGV
CEEEEECCCCCCCCCCHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
DDSKKLTPARRDDLFTLIQGTALAIGVGVADHKVIDRINILQATLAAMREAVECLTDPPD
CCCCCCCCCHHHHHHHHHHCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
YLLVDGISPVPLPVAQQTIKKGDSASISIAAASIIAKVTRDRLMAEYDQLYPGYGFAEHK
EEEECCCCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCC
GYGSASHMAAIAALGPSPIHRTTFRGVREHLGESRCR
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SLTLFDHEPQPAVWDFESRAARRGYRTIAGIDEAGRGPLAGPVVAAAVILPFGTDLPGV
EEEEECCCCCCCCCCHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
DDSKKLTPARRDDLFTLIQGTALAIGVGVADHKVIDRINILQATLAAMREAVECLTDPPD
CCCCCCCCCHHHHHHHHHHCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
YLLVDGISPVPLPVAQQTIKKGDSASISIAAASIIAKVTRDRLMAEYDQLYPGYGFAEHK
EEEECCCCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCC
GYGSASHMAAIAALGPSPIHRTTFRGVREHLGESRCR
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA