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The map label for this gene is gmd [H]

Identifier: 39995733

GI number: 39995733

Start: 663160

End: 664209

Strand: Direct

Name: gmd [H]

Synonym: GSU0626

Alternate gene names: 39995733

Gene position: 663160-664209 (Clockwise)

Preceding gene: 39995732

Following gene: 39995734

Centisome position: 17.39

GC content: 63.81

Gene sequence:

>1050_bases
GTGAAACGCGCACTCATAACCGGCATTTCTGGCCAGGACGGCTCGTACCTGGCGGAACTGCTCCTTGGCAAGGGATACGA
GGTTCACGGCGTCATCCGGCGTTCGTCATCATTCAATACCGGGCGGATCGACCACCTCTATCGGGACCCCCACGAGCCCG
ACGTGCGGCTTTTCCTCCACTACGGCGACTTGAACGACGCCAGCTCCATCAACCGGGTCCTGCGCGAGGTGCGGCCCGAC
GAGATCTACAACCTGGGCGCCCAGAGCCATGTCCGGGTTTCATTCGATGTCCCCGAGTACACCGGGGAAATCGATGCCCT
CGGCACGGTGCGGCTACTGGAAGGCATCCGCGAGACCGGCCTGAACACGAGATTCTACCAGGCCTCGTCCTCCGAGCTGT
ACGGCAAGGTGGTGGAGACGCCCCAGAAGGAAACCACCCCCTTCTACCCCCGCTCACCCTATGCCTGCGCCAAGGCCTAC
GCCTACTACATCACCGTCAACTATCGCGAGAGTTATGGCATGTATGCCTGTAACGGCATTCTGTTCAACCATGAGTCGCC
GCGTCGCGGCGAGACGTTCGTCACCCGGAAGATCACCCGGGCTGCCGGCCGGATCAAGACGGGTCTTCAGGATCGGCTCT
ATCTCGGGAATCTGGATGCCAAGCGCGACTGGGGCTTTGCCGGGGACTACGTGGAAGCCATGTGGCTCATGCTCCAGCAG
CAGGAGGCCGACGACTTCGTGGTGGCCACGGGCGAGACCTGGTCGGTGCGGGAGTTCGCCGAACGGGTCTTCGCGCGGCT
CGGCATGCCCCTTGAGTGGCAGGGGAAAGGGGGGCACGAGAAGGGGATCGACACGAACAGCGGGAAGATCGTGATCGAGA
TCGACCCCCGCTATTTCCGGCCGGCGGAGGTGGACCTGCTGCTGGGCGATCCTGCCAAGGCCCGGCGCCAGCTCGGCTGG
CAGCCCCGGGTGGACTTCCAGGGGCTCGTGGATATGATGACCGATGCGGACCTGGCATTGGCCGAGCGGGAGAAGCGGGC
CGATGGATAG

Upstream 100 bases:

>100_bases
CATTGCGGGAAGAACTCCTGCAGCGGGGCGTGGCTTCGGAACTTCTGCACCAAGCCGGCGAGCCGGCCGGGGAAACTCAG
TCAATCAGGGAGGAACATCC

Downstream 100 bases:

>100_bases
GAACGCCAGGATCTACGTTGCCGGCCACCGGGGATTGGTGGGGTCGGCCATTGTGCGGAAACTGACCGCAGAGGGATACG
GGAACCTGCTCCTGCGCACG

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MKRALITGISGQDGSYLAELLLGKGYEVHGVIRRSSSFNTGRIDHLYRDPHEPDVRLFLHYGDLNDASSINRVLREVRPD
EIYNLGAQSHVRVSFDVPEYTGEIDALGTVRLLEGIRETGLNTRFYQASSSELYGKVVETPQKETTPFYPRSPYACAKAY
AYYITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAAGRIKTGLQDRLYLGNLDAKRDWGFAGDYVEAMWLMLQQ
QEADDFVVATGETWSVREFAERVFARLGMPLEWQGKGGHEKGIDTNSGKIVIEIDPRYFRPAEVDLLLGDPAKARRQLGW
QPRVDFQGLVDMMTDADLALAEREKRADG

Sequences:

>Translated_349_residues
MKRALITGISGQDGSYLAELLLGKGYEVHGVIRRSSSFNTGRIDHLYRDPHEPDVRLFLHYGDLNDASSINRVLREVRPD
EIYNLGAQSHVRVSFDVPEYTGEIDALGTVRLLEGIRETGLNTRFYQASSSELYGKVVETPQKETTPFYPRSPYACAKAY
AYYITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAAGRIKTGLQDRLYLGNLDAKRDWGFAGDYVEAMWLMLQQ
QEADDFVVATGETWSVREFAERVFARLGMPLEWQGKGGHEKGIDTNSGKIVIEIDPRYFRPAEVDLLLGDPAKARRQLGW
QPRVDFQGLVDMMTDADLALAEREKRADG
>Mature_349_residues
MKRALITGISGQDGSYLAELLLGKGYEVHGVIRRSSSFNTGRIDHLYRDPHEPDVRLFLHYGDLNDASSINRVLREVRPD
EIYNLGAQSHVRVSFDVPEYTGEIDALGTVRLLEGIRETGLNTRFYQASSSELYGKVVETPQKETTPFYPRSPYACAKAY
AYYITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAAGRIKTGLQDRLYLGNLDAKRDWGFAGDYVEAMWLMLQQ
QEADDFVVATGETWSVREFAERVFARLGMPLEWQGKGGHEKGIDTNSGKIVIEIDPRYFRPAEVDLLLGDPAKARRQLGW
QPRVDFQGLVDMMTDADLALAEREKRADG

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=340, Percent_Identity=60.2941176470588, Blast_Score=438, Evalue=1e-123,
Organism=Escherichia coli, GI1788366, Length=354, Percent_Identity=63.2768361581921, Blast_Score=473, Evalue=1e-134,
Organism=Escherichia coli, GI48994969, Length=341, Percent_Identity=26.099706744868, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788353, Length=306, Percent_Identity=24.8366013071895, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17507723, Length=350, Percent_Identity=59.7142857142857, Blast_Score=439, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17539424, Length=350, Percent_Identity=58.8571428571429, Blast_Score=435, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI133901790, Length=350, Percent_Identity=58.8571428571429, Blast_Score=434, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI133901786, Length=350, Percent_Identity=58.8571428571429, Blast_Score=434, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17539422, Length=350, Percent_Identity=58.8571428571429, Blast_Score=434, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI133901788, Length=350, Percent_Identity=58.8571428571429, Blast_Score=434, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24158427, Length=345, Percent_Identity=61.4492753623188, Blast_Score=456, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 39584; Mature: 39584

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRALITGISGQDGSYLAELLLGKGYEVHGVIRRSSSFNTGRIDHLYRDPHEPDVRLFLH
CCCEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHCCCCCCCEEEEEE
YGDLNDASSINRVLREVRPDEIYNLGAQSHVRVSFDVPEYTGEIDALGTVRLLEGIRETG
ECCCCCHHHHHHHHHHCCCHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCC
LNTRFYQASSSELYGKVVETPQKETTPFYPRSPYACAKAYAYYITVNYRESYGMYACNGI
CCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCCHHHHEEEEEEEEEEEECCCCEEEEEEE
LFNHESPRRGETFVTRKITRAAGRIKTGLQDRLYLGNLDAKRDWGFAGDYVEAMWLMLQQ
EECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHH
QEADDFVVATGETWSVREFAERVFARLGMPLEWQGKGGHEKGIDTNSGKIVIEIDPRYFR
CCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCEEEEEECCCCCC
PAEVDLLLGDPAKARRQLGWQPRVDFQGLVDMMTDADLALAEREKRADG
CCEEEEEECCCHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKRALITGISGQDGSYLAELLLGKGYEVHGVIRRSSSFNTGRIDHLYRDPHEPDVRLFLH
CCCEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHCCCCCCCEEEEEE
YGDLNDASSINRVLREVRPDEIYNLGAQSHVRVSFDVPEYTGEIDALGTVRLLEGIRETG
ECCCCCHHHHHHHHHHCCCHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCC
LNTRFYQASSSELYGKVVETPQKETTPFYPRSPYACAKAYAYYITVNYRESYGMYACNGI
CCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCCHHHHEEEEEEEEEEEECCCCEEEEEEE
LFNHESPRRGETFVTRKITRAAGRIKTGLQDRLYLGNLDAKRDWGFAGDYVEAMWLMLQQ
EECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHH
QEADDFVVATGETWSVREFAERVFARLGMPLEWQGKGGHEKGIDTNSGKIVIEIDPRYFR
CCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCEEEEEECCCCCC
PAEVDLLLGDPAKARRQLGWQPRVDFQGLVDMMTDADLALAEREKRADG
CCEEEEEECCCHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9098074; 10521656 [H]