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The map label for this gene is fcl [H]

Identifier: 39995734

GI number: 39995734

Start: 664202

End: 665146

Strand: Direct

Name: fcl [H]

Synonym: GSU0627

Alternate gene names: 39995734

Gene position: 664202-665146 (Clockwise)

Preceding gene: 39995733

Following gene: 39995735

Centisome position: 17.41

GC content: 62.75

Gene sequence:

>945_bases
ATGGATAGGAACGCCAGGATCTACGTTGCCGGCCACCGGGGATTGGTGGGGTCGGCCATTGTGCGGAAACTGACCGCAGA
GGGATACGGGAACCTGCTCCTGCGCACGAGCGGTGAGCTGGACCTGCGGGACCAGGCCGCTGTGGCGGCGTTCTTCGCTG
CGGAACAGCCCGATTACGTCTTTCTCGCCGCGGCCAAGGTGGGGGGCATCGTCGCCAACAACACCTATCCGGCCGAGTTC
ATCTACGACAACCTGATGATTGAGGCCAACGTCATCCACAGCTCCTATCGCACCGGCGTGTCGAAGCTCCTCTTCCTGGG
GAGCACCTGCATCTACCCCAAGATGGCGTCCCAGCCAATCCGCGAGGAGTATCTCCTGACCGGTCCACTGGAGCCCACCA
ACGAAGCCTACGCCATCGCCAAGATCGCCGGCATATCCCTGTGCCGCTCCTACAACCGGCAGTACGGCACCCGCTTCATT
GCGGCCATGCCGACCAACCTTTACGGTCCCAACGACAACTTCGATCTTGAGAAGTCCCATGTCCTCCCCGCTCTGATCCG
CAAGTTCCACGAGGCAAAGATCGCCGGAGCTCCGACGGTCACGGTCTGGGGAACAGGGGCGCCGCTGCGGGAGTTCATCC
ACGTGGATGACGTGGCCGACGCCGCGCTCTATCTCATGCGCCACCACGAAGGCAATGACATCGTCAATATCGGCAGCGGC
GAAGAGATCTCCATTCGGGACCTGGCGCTGCTGGTTAAAATAGTCGTGGGCTTCGAAGGTGAGCTCGTCTTTGACGCGTC
AAAGCCCGACGGCACCCCCCGCAAGCTCTCGGACGTGTCCCGGCTGCATTCGCTGGGCTGGCGGCACCGGATCGGGCTGG
AGGACGGGGTGCGGGAAACGTACGAGTGGTTTGTCGGCCAGGGGTTCCCCGCGGAGACGATGTGA

Upstream 100 bases:

>100_bases
CGGCGCCAGCTCGGCTGGCAGCCCCGGGTGGACTTCCAGGGGCTCGTGGATATGATGACCGATGCGGACCTGGCATTGGC
CGAGCGGGAGAAGCGGGCCG

Downstream 100 bases:

>100_bases
TCCCTGTTCCGGTGAAAAACATCGCCCGGTTCCTGATGCACCGGAGAGCGTGGCGGTTCGTTTCGTTCGACTACTGCCCC
GTCTGCGACACCCGGAGCAT

Product: GDP-fucose synthetase

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MDRNARIYVAGHRGLVGSAIVRKLTAEGYGNLLLRTSGELDLRDQAAVAAFFAAEQPDYVFLAAAKVGGIVANNTYPAEF
IYDNLMIEANVIHSSYRTGVSKLLFLGSTCIYPKMASQPIREEYLLTGPLEPTNEAYAIAKIAGISLCRSYNRQYGTRFI
AAMPTNLYGPNDNFDLEKSHVLPALIRKFHEAKIAGAPTVTVWGTGAPLREFIHVDDVADAALYLMRHHEGNDIVNIGSG
EEISIRDLALLVKIVVGFEGELVFDASKPDGTPRKLSDVSRLHSLGWRHRIGLEDGVRETYEWFVGQGFPAETM

Sequences:

>Translated_314_residues
MDRNARIYVAGHRGLVGSAIVRKLTAEGYGNLLLRTSGELDLRDQAAVAAFFAAEQPDYVFLAAAKVGGIVANNTYPAEF
IYDNLMIEANVIHSSYRTGVSKLLFLGSTCIYPKMASQPIREEYLLTGPLEPTNEAYAIAKIAGISLCRSYNRQYGTRFI
AAMPTNLYGPNDNFDLEKSHVLPALIRKFHEAKIAGAPTVTVWGTGAPLREFIHVDDVADAALYLMRHHEGNDIVNIGSG
EEISIRDLALLVKIVVGFEGELVFDASKPDGTPRKLSDVSRLHSLGWRHRIGLEDGVRETYEWFVGQGFPAETM
>Mature_314_residues
MDRNARIYVAGHRGLVGSAIVRKLTAEGYGNLLLRTSGELDLRDQAAVAAFFAAEQPDYVFLAAAKVGGIVANNTYPAEF
IYDNLMIEANVIHSSYRTGVSKLLFLGSTCIYPKMASQPIREEYLLTGPLEPTNEAYAIAKIAGISLCRSYNRQYGTRFI
AAMPTNLYGPNDNFDLEKSHVLPALIRKFHEAKIAGAPTVTVWGTGAPLREFIHVDDVADAALYLMRHHEGNDIVNIGSG
EEISIRDLALLVKIVVGFEGELVFDASKPDGTPRKLSDVSRLHSLGWRHRIGLEDGVRETYEWFVGQGFPAETM

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=307, Percent_Identity=38.1107491856677, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI7657641, Length=329, Percent_Identity=22.7963525835866, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI42516563, Length=311, Percent_Identity=24.4372990353698, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788365, Length=309, Percent_Identity=59.8705501618123, Blast_Score=388, Evalue=1e-109,
Organism=Escherichia coli, GI48994969, Length=345, Percent_Identity=24.9275362318841, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17554428, Length=305, Percent_Identity=33.1147540983607, Blast_Score=164, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI115532424, Length=269, Percent_Identity=25.6505576208178, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17539532, Length=317, Percent_Identity=24.9211356466877, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19922778, Length=312, Percent_Identity=31.4102564102564, Blast_Score=169, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 34562; Mature: 34562

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRNARIYVAGHRGLVGSAIVRKLTAEGYGNLLLRTSGELDLRDQAAVAAFFAAEQPDYV
CCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEE
FLAAAKVGGIVANNTYPAEFIYDNLMIEANVIHSSYRTGVSKLLFLGSTCIYPKMASQPI
EEEEEHHCCEEECCCCCHHHHHCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
REEYLLTGPLEPTNEAYAIAKIAGISLCRSYNRQYGTRFIAAMPTNLYGPNDNFDLEKSH
CCCEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHH
VLPALIRKFHEAKIAGAPTVTVWGTGAPLREFIHVDDVADAALYLMRHHEGNDIVNIGSG
HHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCC
EEISIRDLALLVKIVVGFEGELVFDASKPDGTPRKLSDVSRLHSLGWRHRIGLEDGVRET
CEEHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEECCCHHHHHHH
YEWFVGQGFPAETM
HHHHHCCCCCCCCC
>Mature Secondary Structure
MDRNARIYVAGHRGLVGSAIVRKLTAEGYGNLLLRTSGELDLRDQAAVAAFFAAEQPDYV
CCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEE
FLAAAKVGGIVANNTYPAEFIYDNLMIEANVIHSSYRTGVSKLLFLGSTCIYPKMASQPI
EEEEEHHCCEEECCCCCHHHHHCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
REEYLLTGPLEPTNEAYAIAKIAGISLCRSYNRQYGTRFIAAMPTNLYGPNDNFDLEKSH
CCCEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHH
VLPALIRKFHEAKIAGAPTVTVWGTGAPLREFIHVDDVADAALYLMRHHEGNDIVNIGSG
HHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCC
EEISIRDLALLVKIVVGFEGELVFDASKPDGTPRKLSDVSRLHSLGWRHRIGLEDGVRET
CEEHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEECCCHHHHHHH
YEWFVGQGFPAETM
HHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]