Click here to switch to the map view.

The map label for this gene is purD [H]

Identifier: 39995717

GI number: 39995717

Start: 646167

End: 647438

Strand: Direct

Name: purD [H]

Synonym: GSU0610

Alternate gene names: 39995717

Gene position: 646167-647438 (Clockwise)

Preceding gene: 39995716

Following gene: 39995718

Centisome position: 16.94

GC content: 63.44

Gene sequence:

>1272_bases
ATGAAGGTACTGGTAATCGGAGGGGGGGGCAGGGAGCACGCCCTGGTCTGGAAAATCGCCCAGTCACCCCTGGTAAGCAA
GGTGTACTGCGCGCCGGGCAACCCCGGCATCGGCCTTATCGCCGAGAACGTGTCCCTGGCGGTGGATGACCTGGACGGGC
TCGCCGCCTTTGCGACGGAACAGTCTATCGACCTCACCGTGGTGGGGCCGGAACTACCTCTTTCCCTCGGCATCGTTGAC
CGGTTCGAGGAAAAGGGGCTACTGATCTTCGGCGCCCGCCGGAATGCCGCCATCATCGAAGCGAGCAAGGCGTTTTCGAA
AGACCTGATGAAAAAGTATCAGGTACCCACCGCCGCCTATGACGTCTTTACCGAGGTGGAGCCGGCCGTGGCTTTCATCG
ACCGGGTAGGGGTGCCCATCGTCGTCAAGGCGGACGGGCTGGCCGCCGGCAAAGGGGTTATCATCGCCCATACCCGTGAG
GAAGCCGTTGGCGCCGTGATGGACATGCTGTCGGGCAACGCCTTCGGCGATGCCGGTTCGCGGGTGGTCATCGAAGAGTT
TCTCACCGGGGAGGAGGCATCGTTTCTCGCCTTCACCGACGGCAAGAACATCATTCCCCTGGCCAGCGCCCAGGACCACA
AGGCGGTCTTTGACGGCGACACGGGACCCAATACCGGCGGCATGGGCGCCTATTCACCCGCGCCGGTAGTCACCCCTGCC
ATTCATGATAAGGTCATGGCCGAGGTGATGCGCCGGACCGTGGACGGCATGGCTGCCGAGGGGCGTCCTTATCGCGGGGT
GCTGTATGCCGGTCTCATGATCAACGGTGACCAGGTGAAAACCCTCGAGTTCAACGCGCGCTTCGGCGACCCAGAGTGCC
AGCCGCTGCTCATGCGGATGAAGTCGGACATCGTGCCCGTGCTCCTTGCGGTTGCCCGTGGCGACCTGTCGGGCATTGAG
CTCGAATGGCATGACATGGCTGCCGTCTGTGTCGTCATGGCTTCCGGGGGGTATCCCGCCGATTACCGCAAGGGAGACGA
GATCCGGGGGCTGGAGGATGCGGCTCGCATGGAGGATCTGTTCGTGTTCCACGCCGGCACATCCCGGAAAAACGGCCGTA
TCGTCACCAGCGGGGGGCGGGTGCTTGGGGTTACTGCCCTTGGCGCTACGGTGGGTGAGGCCATTGATCGCGCCTACCGG
GGCGTTGCGGCAATCTCATGGGACGGCGTCCACTACCGCAAAGACATCGGCGCCAAGGCGCTCACACGTTGA

Upstream 100 bases:

>100_bases
GAGGTGATCGCCGCCGCCGACGAGCACGGCATGGCGATGGTATTCACCGGCATGAGGCATTTCAGACACTGAGACAGGGA
ACGATCACGGGGAGGAACCC

Downstream 100 bases:

>100_bases
CAGGGGAGGTCGCGATGGAAAACCCGCAGGTACTCATCATTATGGGAAGTGATTCGGACGTTCCCGTCATGGAAGAAGCG
GCAAAGGCCCTTGATGCTTT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MKVLVIGGGGREHALVWKIAQSPLVSKVYCAPGNPGIGLIAENVSLAVDDLDGLAAFATEQSIDLTVVGPELPLSLGIVD
RFEEKGLLIFGARRNAAIIEASKAFSKDLMKKYQVPTAAYDVFTEVEPAVAFIDRVGVPIVVKADGLAAGKGVIIAHTRE
EAVGAVMDMLSGNAFGDAGSRVVIEEFLTGEEASFLAFTDGKNIIPLASAQDHKAVFDGDTGPNTGGMGAYSPAPVVTPA
IHDKVMAEVMRRTVDGMAAEGRPYRGVLYAGLMINGDQVKTLEFNARFGDPECQPLLMRMKSDIVPVLLAVARGDLSGIE
LEWHDMAAVCVVMASGGYPADYRKGDEIRGLEDAARMEDLFVFHAGTSRKNGRIVTSGGRVLGVTALGATVGEAIDRAYR
GVAAISWDGVHYRKDIGAKALTR

Sequences:

>Translated_423_residues
MKVLVIGGGGREHALVWKIAQSPLVSKVYCAPGNPGIGLIAENVSLAVDDLDGLAAFATEQSIDLTVVGPELPLSLGIVD
RFEEKGLLIFGARRNAAIIEASKAFSKDLMKKYQVPTAAYDVFTEVEPAVAFIDRVGVPIVVKADGLAAGKGVIIAHTRE
EAVGAVMDMLSGNAFGDAGSRVVIEEFLTGEEASFLAFTDGKNIIPLASAQDHKAVFDGDTGPNTGGMGAYSPAPVVTPA
IHDKVMAEVMRRTVDGMAAEGRPYRGVLYAGLMINGDQVKTLEFNARFGDPECQPLLMRMKSDIVPVLLAVARGDLSGIE
LEWHDMAAVCVVMASGGYPADYRKGDEIRGLEDAARMEDLFVFHAGTSRKNGRIVTSGGRVLGVTALGATVGEAIDRAYR
GVAAISWDGVHYRKDIGAKALTR
>Mature_423_residues
MKVLVIGGGGREHALVWKIAQSPLVSKVYCAPGNPGIGLIAENVSLAVDDLDGLAAFATEQSIDLTVVGPELPLSLGIVD
RFEEKGLLIFGARRNAAIIEASKAFSKDLMKKYQVPTAAYDVFTEVEPAVAFIDRVGVPIVVKADGLAAGKGVIIAHTRE
EAVGAVMDMLSGNAFGDAGSRVVIEEFLTGEEASFLAFTDGKNIIPLASAQDHKAVFDGDTGPNTGGMGAYSPAPVVTPA
IHDKVMAEVMRRTVDGMAAEGRPYRGVLYAGLMINGDQVKTLEFNARFGDPECQPLLMRMKSDIVPVLLAVARGDLSGIE
LEWHDMAAVCVVMASGGYPADYRKGDEIRGLEDAARMEDLFVFHAGTSRKNGRIVTSGGRVLGVTALGATVGEAIDRAYR
GVAAISWDGVHYRKDIGAKALTR

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=424, Percent_Identity=49.5283018867925, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI4503915, Length=424, Percent_Identity=49.5283018867925, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI209869995, Length=424, Percent_Identity=49.5283018867925, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI209869993, Length=424, Percent_Identity=49.5283018867925, Blast_Score=402, Evalue=1e-112,
Organism=Escherichia coli, GI1790438, Length=429, Percent_Identity=57.8088578088578, Blast_Score=471, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17567511, Length=424, Percent_Identity=42.688679245283, Blast_Score=300, Evalue=1e-81,
Organism=Saccharomyces cerevisiae, GI6321203, Length=440, Percent_Identity=42.9545454545455, Blast_Score=335, Evalue=9e-93,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=45.3488372093023, Blast_Score=325, Evalue=3e-89,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=45.3488372093023, Blast_Score=325, Evalue=4e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 44732; Mature: 44732

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVIGGGGREHALVWKIAQSPLVSKVYCAPGNPGIGLIAENVSLAVDDLDGLAAFATE
CEEEEEECCCCCCEEEEEECCCCCCEEEEECCCCCCCEEEECCCEEEEECCCCHHHHHCC
QSIDLTVVGPELPLSLGIVDRFEEKGLLIFGARRNAAIIEASKAFSKDLMKKYQVPTAAY
CCEEEEEECCCCCEEECHHHHHCCCCEEEEECCCCCEEEEHHHHHHHHHHHHHCCCHHHH
DVFTEVEPAVAFIDRVGVPIVVKADGLAAGKGVIIAHTREEAVGAVMDMLSGNAFGDAGS
HHHHHCCHHHHHHHHCCCCEEEEECCCCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCC
RVVIEEFLTGEEASFLAFTDGKNIIPLASAQDHKAVFDGDTGPNTGGMGAYSPAPVVTPA
EEEEHHHHCCCCCEEEEEECCCCEEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCCHH
IHDKVMAEVMRRTVDGMAAEGRPYRGVLYAGLMINGDQVKTLEFNARFGDPECQPLLMRM
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCEEEEEEEECCCCCCCHHHHHHHH
KSDIVPVLLAVARGDLSGIELEWHDMAAVCVVMASGGYPADYRKGDEIRGLEDAARMEDL
HHHHHHHHHHHHCCCCCCEEEEHHHHEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHEEE
FVFHAGTSRKNGRIVTSGGRVLGVTALGATVGEAIDRAYRGVAAISWDGVHYRKDIGAKA
EEEECCCCCCCCEEEECCCCEEEEEHHHHHHHHHHHHHHCCEEEEEECCEEEHHHCCHHH
LTR
HCC
>Mature Secondary Structure
MKVLVIGGGGREHALVWKIAQSPLVSKVYCAPGNPGIGLIAENVSLAVDDLDGLAAFATE
CEEEEEECCCCCCEEEEEECCCCCCEEEEECCCCCCCEEEECCCEEEEECCCCHHHHHCC
QSIDLTVVGPELPLSLGIVDRFEEKGLLIFGARRNAAIIEASKAFSKDLMKKYQVPTAAY
CCEEEEEECCCCCEEECHHHHHCCCCEEEEECCCCCEEEEHHHHHHHHHHHHHCCCHHHH
DVFTEVEPAVAFIDRVGVPIVVKADGLAAGKGVIIAHTREEAVGAVMDMLSGNAFGDAGS
HHHHHCCHHHHHHHHCCCCEEEEECCCCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCC
RVVIEEFLTGEEASFLAFTDGKNIIPLASAQDHKAVFDGDTGPNTGGMGAYSPAPVVTPA
EEEEHHHHCCCCCEEEEEECCCCEEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCCHH
IHDKVMAEVMRRTVDGMAAEGRPYRGVLYAGLMINGDQVKTLEFNARFGDPECQPLLMRM
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCEEEEEEEECCCCCCCHHHHHHHH
KSDIVPVLLAVARGDLSGIELEWHDMAAVCVVMASGGYPADYRKGDEIRGLEDAARMEDL
HHHHHHHHHHHHCCCCCCEEEEHHHHEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHEEE
FVFHAGTSRKNGRIVTSGGRVLGVTALGATVGEAIDRAYRGVAAISWDGVHYRKDIGAKA
EEEECCCCCCCCEEEECCCCEEEEEHHHHHHHHHHHHHHCCEEEEEECCEEEHHHCCHHH
LTR
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]