Click here to switch to the map view.

The map label for this gene is purH

Identifier: 39995716

GI number: 39995716

Start: 644573

End: 646138

Strand: Direct

Name: purH

Synonym: GSU0609

Alternate gene names: 39995716

Gene position: 644573-646138 (Clockwise)

Preceding gene: 39995715

Following gene: 39995717

Centisome position: 16.9

GC content: 64.62

Gene sequence:

>1566_bases
ATGGCAAAGATAACACGTGCGCTCATCAGCGTCTCGGACAAGACCGGCATCCTTGATTTCGCCCGGGAACTGGCCGGCTA
CGGCGTGGAGATCCTCTCCACCGGCGGTACCGCAAAGCTTCTCCGCGACGCGGGACTCGCGGTCAAGGACGTCTCCGATT
TTACCGGCTTTCCGGAGATGCTCGACGGCCGGGTCAAGACGCTTCACCCCAAGGTCCACGGGGGCCTTCTGGGAATGCGC
TCCAACCCAGACCATGTGGCAACCATGAAGGCGCACGGCATCGAGCCCATCGACTTGGTGGTGGTGAACCTCTACCCCTT
CGAGGCCACCGTGGCCAAGCCCGAATGCACCCTGGAAGATGCCATCGAGAACATCGATATCGGCGGTCCCACCATGCTCC
GCTCCGCGGCCAAGAACAACGCCGACGTGACCGTGCTCGTGGATCCGGCGGACTATCGGCCGGTTCTCGATGAAATGAAG
GCATCCGGCGGCGCCGTGTCCCGGGAGACCAACTTTTGCCTGGCGGTGAAAGTCTACCAGCACACTGCAGCCTATGACGG
CGCCATTTCCAACTGGCTCGGCGCCCGGACCGGCGAAGGGATCGCCGCCTATCCCGACACCGTCACGCTTCAGTTCAGAA
AGGCCCAGGAGATGCGCTACGGCGAGAACCCCCACCAGGGTGCCGCCTTCTATGTGGAGCGCCAGGTGAAGGAAGCGTCC
GTCGCCACGGCCCGCCAGCTCCAGGGCAAGGAGCTTTCCTACAACAACATCGCCGACACCGACGCGGCCCTGGAGTGCGT
GAAGCAGTTTGCCGAAGGCCCCGCCTGCGTCATCGTAAAGCATGCCAACCCCTGCGGCGTGGCCGTGGGCGGGACGTTGC
TGGAGGCCTACGACCGGGCCTATGCCACCGATCCCGAGTCGGCCTTCGGGGGCATCATCGCCTTCAACCGGGAACTGGAC
GCCGACACGGCGCGGGCAATCTGCGACCGCCAGTTCGTGGAGGTCATCATCGCTCCCGCCGTATCGCCGGAGGCCACGGA
AGTTGTTGCCGCCAAGAAGAACGTGCGTCTCCTGGAGTGCGGCACCTGGCCGGAGAAGCAACAGCCGCGCCTCGACCTGA
AGCGGGTGAACGGCGGCATCCTGGTGCAGGACACCGATCTCGACCTGTACGCCGAACTGAAGGTCGTGACCAAGCGGCAG
CCCACGGAGCAGGAGATGAAGGACCTGCTCTTTGCCTGGCGCGTGGCCAAGTTCGTCAAGTCCAACGCCATTGTCTACGG
CAAGGGCAATATGACCATCGGCGTGGGGGCCGGCCAGATGAGCCGGGTCAACTCGGCCCGCATCGCCGCCATCAAGGCCG
AGCACGCGGGGCTTGAGGTGAAGGGGGCCGTTATGGCGTCCGATGCCTTCTTCCCCTTCCGCGACGGCATCGACAACGCG
GCTGCCGTGGGCATCACCGCGGTTATCCAGCCGGGCGGCAGCATGCGCGACGCTGAGGTGATCGCCGCCGCCGACGAGCA
CGGCATGGCGATGGTATTCACCGGCATGAGGCATTTCAGACACTGA

Upstream 100 bases:

>100_bases
GCCTTCGAGAAACACTTTCTGAAGCACATCGACTTCCCGTCTCGCTAACATCCGCCACGGAGCTCCGTGGTGATCTCAGC
TCATGTCAAAGGGGTAAACC

Downstream 100 bases:

>100_bases
GACAGGGAACGATCACGGGGAGGAACCCATGAAGGTACTGGTAATCGGAGGGGGGGGCAGGGAGCACGCCCTGGTCTGGA
AAATCGCCCAGTCACCCCTG

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MAKITRALISVSDKTGILDFARELAGYGVEILSTGGTAKLLRDAGLAVKDVSDFTGFPEMLDGRVKTLHPKVHGGLLGMR
SNPDHVATMKAHGIEPIDLVVVNLYPFEATVAKPECTLEDAIENIDIGGPTMLRSAAKNNADVTVLVDPADYRPVLDEMK
ASGGAVSRETNFCLAVKVYQHTAAYDGAISNWLGARTGEGIAAYPDTVTLQFRKAQEMRYGENPHQGAAFYVERQVKEAS
VATARQLQGKELSYNNIADTDAALECVKQFAEGPACVIVKHANPCGVAVGGTLLEAYDRAYATDPESAFGGIIAFNRELD
ADTARAICDRQFVEVIIAPAVSPEATEVVAAKKNVRLLECGTWPEKQQPRLDLKRVNGGILVQDTDLDLYAELKVVTKRQ
PTEQEMKDLLFAWRVAKFVKSNAIVYGKGNMTIGVGAGQMSRVNSARIAAIKAEHAGLEVKGAVMASDAFFPFRDGIDNA
AAVGITAVIQPGGSMRDAEVIAAADEHGMAMVFTGMRHFRH

Sequences:

>Translated_521_residues
MAKITRALISVSDKTGILDFARELAGYGVEILSTGGTAKLLRDAGLAVKDVSDFTGFPEMLDGRVKTLHPKVHGGLLGMR
SNPDHVATMKAHGIEPIDLVVVNLYPFEATVAKPECTLEDAIENIDIGGPTMLRSAAKNNADVTVLVDPADYRPVLDEMK
ASGGAVSRETNFCLAVKVYQHTAAYDGAISNWLGARTGEGIAAYPDTVTLQFRKAQEMRYGENPHQGAAFYVERQVKEAS
VATARQLQGKELSYNNIADTDAALECVKQFAEGPACVIVKHANPCGVAVGGTLLEAYDRAYATDPESAFGGIIAFNRELD
ADTARAICDRQFVEVIIAPAVSPEATEVVAAKKNVRLLECGTWPEKQQPRLDLKRVNGGILVQDTDLDLYAELKVVTKRQ
PTEQEMKDLLFAWRVAKFVKSNAIVYGKGNMTIGVGAGQMSRVNSARIAAIKAEHAGLEVKGAVMASDAFFPFRDGIDNA
AAVGITAVIQPGGSMRDAEVIAAADEHGMAMVFTGMRHFRH
>Mature_520_residues
AKITRALISVSDKTGILDFARELAGYGVEILSTGGTAKLLRDAGLAVKDVSDFTGFPEMLDGRVKTLHPKVHGGLLGMRS
NPDHVATMKAHGIEPIDLVVVNLYPFEATVAKPECTLEDAIENIDIGGPTMLRSAAKNNADVTVLVDPADYRPVLDEMKA
SGGAVSRETNFCLAVKVYQHTAAYDGAISNWLGARTGEGIAAYPDTVTLQFRKAQEMRYGENPHQGAAFYVERQVKEASV
ATARQLQGKELSYNNIADTDAALECVKQFAEGPACVIVKHANPCGVAVGGTLLEAYDRAYATDPESAFGGIIAFNRELDA
DTARAICDRQFVEVIIAPAVSPEATEVVAAKKNVRLLECGTWPEKQQPRLDLKRVNGGILVQDTDLDLYAELKVVTKRQP
TEQEMKDLLFAWRVAKFVKSNAIVYGKGNMTIGVGAGQMSRVNSARIAAIKAEHAGLEVKGAVMASDAFFPFRDGIDNAA
AVGITAVIQPGGSMRDAEVIAAADEHGMAMVFTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=514, Percent_Identity=38.715953307393, Blast_Score=291, Evalue=9e-79,
Organism=Escherichia coli, GI1790439, Length=527, Percent_Identity=67.3624288425047, Blast_Score=720, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=509, Percent_Identity=35.1669941060904, Blast_Score=256, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI71985574, Length=353, Percent_Identity=26.628895184136, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71985556, Length=60, Percent_Identity=51.6666666666667, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323768, Length=492, Percent_Identity=39.0243902439024, Blast_Score=278, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6323056, Length=496, Percent_Identity=36.6935483870968, Blast_Score=272, Evalue=9e-74,
Organism=Drosophila melanogaster, GI24649832, Length=611, Percent_Identity=36.6612111292962, Blast_Score=337, Evalue=1e-92,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_GEOSL (Q74FJ9)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_951667.1
- HSSP:   P31939
- ProteinModelPortal:   Q74FJ9
- SMR:   Q74FJ9
- GeneID:   2685298
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU0609
- NMPDR:   fig|243231.1.peg.605
- TIGR:   GSU0609
- HOGENOM:   HBG498048
- OMA:   ASDGFFP
- ProtClustDB:   PRK00881
- BioCyc:   GSUL243231:GSU_0609-MONOMER
- BRENDA:   2.1.2.3
- BRENDA:   3.5.4.10
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 55966; Mature: 55835

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKITRALISVSDKTGILDFARELAGYGVEILSTGGTAKLLRDAGLAVKDVSDFTGFPEM
CCHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHCCCEEEECHHHCCCHHH
LDGRVKTLHPKVHGGLLGMRSNPDHVATMKAHGIEPIDLVVVNLYPFEATVAKPECTLED
HCCCCEEECCHHCCCEEECCCCCCCEEEEHHCCCCCEEEEEEEEECCEEECCCCCCCHHH
AIENIDIGGPTMLRSAAKNNADVTVLVDPADYRPVLDEMKASGGAVSRETNFCLAVKVYQ
HHHHCCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCCCCCCCCCEEEEEEEEH
HTAAYDGAISNWLGARTGEGIAAYPDTVTLQFRKAQEMRYGENPHQGAAFYVERQVKEAS
HHHHHCCHHHHHHCCCCCCCEEECCCEEEEEEEHHHHHHCCCCCCCCCEEEEHHHHHHHH
VATARQLQGKELSYNNIADTDAALECVKQFAEGPACVIVKHANPCGVAVGGTLLEAYDRA
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCEEHHHHHHHHHHHH
YATDPESAFGGIIAFNRELDADTARAICDRQFVEVIIAPAVSPEATEVVAAKKNVRLLEC
HCCCCCHHCCCEEEECCCCCCHHHHHHHHHHHHHHHEECCCCCCHHHHHHHCCCEEEEEC
GTWPEKQQPRLDLKRVNGGILVQDTDLDLYAELKVVTKRQPTEQEMKDLLFAWRVAKFVK
CCCCCCCCCCCCCEECCCCEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
SNAIVYGKGNMTIGVGAGQMSRVNSARIAAIKAEHAGLEVKGAVMASDAFFPFRDGIDNA
CCEEEEECCCEEEEECCCCHHHCCCCEEEEEEECCCCCEEEEEEEECCCCCCHHCCCCCH
AAVGITAVIQPGGSMRDAEVIAAADEHGMAMVFTGMRHFRH
HHEEEEEEECCCCCCCCCEEEEECCCCCEEEEEEHHHHHCC
>Mature Secondary Structure 
AKITRALISVSDKTGILDFARELAGYGVEILSTGGTAKLLRDAGLAVKDVSDFTGFPEM
CHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHCCCEEEECHHHCCCHHH
LDGRVKTLHPKVHGGLLGMRSNPDHVATMKAHGIEPIDLVVVNLYPFEATVAKPECTLED
HCCCCEEECCHHCCCEEECCCCCCCEEEEHHCCCCCEEEEEEEEECCEEECCCCCCCHHH
AIENIDIGGPTMLRSAAKNNADVTVLVDPADYRPVLDEMKASGGAVSRETNFCLAVKVYQ
HHHHCCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCCCCCCCCCEEEEEEEEH
HTAAYDGAISNWLGARTGEGIAAYPDTVTLQFRKAQEMRYGENPHQGAAFYVERQVKEAS
HHHHHCCHHHHHHCCCCCCCEEECCCEEEEEEEHHHHHHCCCCCCCCCEEEEHHHHHHHH
VATARQLQGKELSYNNIADTDAALECVKQFAEGPACVIVKHANPCGVAVGGTLLEAYDRA
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCEEHHHHHHHHHHHH
YATDPESAFGGIIAFNRELDADTARAICDRQFVEVIIAPAVSPEATEVVAAKKNVRLLEC
HCCCCCHHCCCEEEECCCCCCHHHHHHHHHHHHHHHEECCCCCCHHHHHHHCCCEEEEEC
GTWPEKQQPRLDLKRVNGGILVQDTDLDLYAELKVVTKRQPTEQEMKDLLFAWRVAKFVK
CCCCCCCCCCCCCEECCCCEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
SNAIVYGKGNMTIGVGAGQMSRVNSARIAAIKAEHAGLEVKGAVMASDAFFPFRDGIDNA
CCEEEEECCCEEEEECCCCHHHCCCCEEEEEEECCCCCEEEEEEEECCCCCCHHCCCCCH
AAVGITAVIQPGGSMRDAEVIAAADEHGMAMVFTGMRHFRH
HHEEEEEEECCCCCCCCCEEEEECCCCCEEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA