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The map label for this gene is thiDE

Identifier: 39995712

GI number: 39995712

Start: 639288

End: 640760

Strand: Reverse

Name: thiDE

Synonym: GSU0605

Alternate gene names: 39995712

Gene position: 640760-639288 (Counterclockwise)

Preceding gene: 39995744

Following gene: 39995711

Centisome position: 16.8

GC content: 66.67

Gene sequence:

>1473_bases
ATGGCCTCTAACGGTCACACGCTTCGGCTCGTCATAAACCGGGACAAGCACGACTCGGTCATCCGGGGGCTCTACCTGGT
GACCGATCACGACGACAACCTCATCCCGCGGGTTGAGGCGGCCATCGACGGCGGCGCCCGAGTGGTCCAGTACCGCAACA
AGAATCAGGACCGGGAGAGCCGGCTCGCCCTCGGCCTGGAGCTGCGGGAGCTGTGCCGCAGGCGGAGCATCCCCTTCATC
GTGAACGACGACCTGGAGATGGCCGTGAGCCTCAAGGCCGACGGGCTTCACCTGGGCCAGGGTGACGGCGATCCCCGCGA
AGCGCGACGCGTTCTCGGCCCCGGCAAGATCATCGGCGTGTCCACCCACACCCTGAGCGAAGCCTTGGAGGCCCAGGCGG
CCGGGGTGGACTACATCGGCCTCGGCGCCATGTTCCCCTCCCGGAGTAAGGAGGTCGAGCATGTGGCCGGATCGGAGCTG
CTCGCGGCCATCCGGAGTTCCATCAGCATCCCCATTGTCGCCATCGGCGGCATTACCCGCGATAACGGCGCCAGCGTCAT
TGATGCCGGTGCCGATGCCGTGGCAGTCATATCGGCGGTTCTGTCCCATCCCGATCCGGCCCTGGCGGCCACCGAGATAG
CACTGCTCTTCAACCGGCGCGCACCGTTTCCGCGCGGCTCCGTCCTCACCGTGGCCGGCAGCGATTCGGGGGGAGGCGCG
GGCATCCAGGCAGACCTGAAAACCGTCACCCTGCTCGGGAGCTATGGCTCGTCGGTCCTCACGGCCCTGACAGCCCAGAA
CACCCGGGGGGTCAGCGGCATCCACGGCGTACCGCCGGCGTTCGTTGCCGACCAGCTCGACGCGGTCTTCTCCGACATTC
CCGTGGATGTGGTCAAGACCGGCATGCTCTTCTCGGCGGAAACCATCGTCGCAATCGCCGCGAAGCTTACCGAGTACCGG
CGGCGAATGGTAGTGGTCGATCCCGTTATGGTGGCCAAGGGGGGGGCGAACCTGATCGACCGCGGAGCGGTAAGCGTGCT
CAAGGAGCGGCTCTTTCCCCTCGCCTACCTCGTTACCCCCAATATACCCGAGGCCGAGCGGCTCACCGGTGCAAACATCT
CCGACGAAGAATCGATGCGGGAGGCGGCCCGCCGCCTGCACCGGCTGGGGGCACGCAACGTTCTTCTCAAGGGCGGCCAC
CTGCTGGCCGGCGACTCGGTGGACATCCTCTTCGACGGGGCAGCCTTCCACCGCTTCGTCTCACCGCGAATCCTCTCGAA
AAACACCCACGGCACCGGCTGTACCTTCGCCTCGGCCATTGCCACCTATCTGGCCCAGGGCGACCCCCTGCGCGAAGCCA
TCGCCCGGGCCAAACGTTACATCACCGCTGCCATCCGCCTTGCCCAGCCCTTGGGACGCGGACACGGACCGGTCAACCAT
ATCCTCGCCGCGGAGGACGTCAGGGACCGGTGA

Upstream 100 bases:

>100_bases
TTATACCGGCGTAGGGAAGCGGCCAGAAACAATCATCCGTACGTCTTTTCTGGAACCGCGACTTTGCCGTCGCGGTTTTT
TTTTGAGGAGATTTCCTTTC

Downstream 100 bases:

>100_bases
TCGGGGACCGGCAGAAATCACCCGGATAACGACCAAGGAGTATCGACACATGGCTATGACGCAACTGGAATACGCCCGCA
ACGGAATCATCACCGACAAG

Product: thiamine-phosphate pyrophosphorylase/phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MASNGHTLRLVINRDKHDSVIRGLYLVTDHDDNLIPRVEAAIDGGARVVQYRNKNQDRESRLALGLELRELCRRRSIPFI
VNDDLEMAVSLKADGLHLGQGDGDPREARRVLGPGKIIGVSTHTLSEALEAQAAGVDYIGLGAMFPSRSKEVEHVAGSEL
LAAIRSSISIPIVAIGGITRDNGASVIDAGADAVAVISAVLSHPDPALAATEIALLFNRRAPFPRGSVLTVAGSDSGGGA
GIQADLKTVTLLGSYGSSVLTALTAQNTRGVSGIHGVPPAFVADQLDAVFSDIPVDVVKTGMLFSAETIVAIAAKLTEYR
RRMVVVDPVMVAKGGANLIDRGAVSVLKERLFPLAYLVTPNIPEAERLTGANISDEESMREAARRLHRLGARNVLLKGGH
LLAGDSVDILFDGAAFHRFVSPRILSKNTHGTGCTFASAIATYLAQGDPLREAIARAKRYITAAIRLAQPLGRGHGPVNH
ILAAEDVRDR

Sequences:

>Translated_490_residues
MASNGHTLRLVINRDKHDSVIRGLYLVTDHDDNLIPRVEAAIDGGARVVQYRNKNQDRESRLALGLELRELCRRRSIPFI
VNDDLEMAVSLKADGLHLGQGDGDPREARRVLGPGKIIGVSTHTLSEALEAQAAGVDYIGLGAMFPSRSKEVEHVAGSEL
LAAIRSSISIPIVAIGGITRDNGASVIDAGADAVAVISAVLSHPDPALAATEIALLFNRRAPFPRGSVLTVAGSDSGGGA
GIQADLKTVTLLGSYGSSVLTALTAQNTRGVSGIHGVPPAFVADQLDAVFSDIPVDVVKTGMLFSAETIVAIAAKLTEYR
RRMVVVDPVMVAKGGANLIDRGAVSVLKERLFPLAYLVTPNIPEAERLTGANISDEESMREAARRLHRLGARNVLLKGGH
LLAGDSVDILFDGAAFHRFVSPRILSKNTHGTGCTFASAIATYLAQGDPLREAIARAKRYITAAIRLAQPLGRGHGPVNH
ILAAEDVRDR
>Mature_489_residues
ASNGHTLRLVINRDKHDSVIRGLYLVTDHDDNLIPRVEAAIDGGARVVQYRNKNQDRESRLALGLELRELCRRRSIPFIV
NDDLEMAVSLKADGLHLGQGDGDPREARRVLGPGKIIGVSTHTLSEALEAQAAGVDYIGLGAMFPSRSKEVEHVAGSELL
AAIRSSISIPIVAIGGITRDNGASVIDAGADAVAVISAVLSHPDPALAATEIALLFNRRAPFPRGSVLTVAGSDSGGGAG
IQADLKTVTLLGSYGSSVLTALTAQNTRGVSGIHGVPPAFVADQLDAVFSDIPVDVVKTGMLFSAETIVAIAAKLTEYRR
RMVVVDPVMVAKGGANLIDRGAVSVLKERLFPLAYLVTPNIPEAERLTGANISDEESMREAARRLHRLGARNVLLKGGHL
LAGDSVDILFDGAAFHRFVSPRILSKNTHGTGCTFASAIATYLAQGDPLREAIARAKRYITAAIRLAQPLGRGHGPVNHI
LAAEDVRDR

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiD family

Homologues:

Organism=Escherichia coli, GI1788420, Length=262, Percent_Identity=46.5648854961832, Blast_Score=228, Evalue=1e-60,
Organism=Escherichia coli, GI1790426, Length=193, Percent_Identity=33.6787564766839, Blast_Score=83, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6325378, Length=288, Percent_Identity=35.4166666666667, Blast_Score=163, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6324997, Length=290, Percent_Identity=36.8965517241379, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6324517, Length=301, Percent_Identity=35.2159468438538, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6325042, Length=220, Percent_Identity=31.8181818181818, Blast_Score=103, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIED_GEOSL (P61422)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_951663.1
- ProteinModelPortal:   P61422
- GeneID:   2687129
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU0605
- NMPDR:   fig|243231.1.peg.601
- TIGR:   GSU0605
- HOGENOM:   HBG351127
- OMA:   TLLGSYG
- ProtClustDB:   CLSK827942
- BioCyc:   GSUL243231:GSU_0605-MONOMER
- BRENDA:   2.5.1.3
- BRENDA:   2.7.4.7
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00097
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF08543 Phos_pyr_kin; PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7

Molecular weight: Translated: 51911; Mature: 51780

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: NA

Important sites: BINDING 266-266

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASNGHTLRLVINRDKHDSVIRGLYLVTDHDDNLIPRVEAAIDGGARVVQYRNKNQDRES
CCCCCCEEEEEEECCCCCHHHEEEEEEECCCCCCCCHHHHHHCCCCEEEEECCCCCCHHH
RLALGLELRELCRRRSIPFIVNDDLEMAVSLKADGLHLGQGDGDPREARRVLGPGKIIGV
HHHHHHHHHHHHHHCCCCEEECCCCEEEEEEECCCEEECCCCCCHHHHHHHCCCCCEEEE
STHTLSEALEAQAAGVDYIGLGAMFPSRSKEVEHVAGSELLAAIRSSISIPIVAIGGITR
EHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCEEC
DNGASVIDAGADAVAVISAVLSHPDPALAATEIALLFNRRAPFPRGSVLTVAGSDSGGGA
CCCCCEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCC
GIQADLKTVTLLGSYGSSVLTALTAQNTRGVSGIHGVPPAFVADQLDAVFSDIPVDVVKT
CEEECCEEEEEEHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHC
GMLFSAETIVAIAAKLTEYRRRMVVVDPVMVAKGGANLIDRGAVSVLKERLFPLAYLVTP
CCCCCHHHHHHHHHHHHHHHCCEEEECCEEEECCCCCHHHHHHHHHHHHHHCCEEEEECC
NIPEAERLTGANISDEESMREAARRLHRLGARNVLLKGGHLLAGDSVDILFDGAAFHRFV
CCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCEEEECCCEEEEECCHHHHHHC
SPRILSKNTHGTGCTFASAIATYLAQGDPLREAIARAKRYITAAIRLAQPLGRGHGPVNH
CCHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
ILAAEDVRDR
HEECHHCCCC
>Mature Secondary Structure 
ASNGHTLRLVINRDKHDSVIRGLYLVTDHDDNLIPRVEAAIDGGARVVQYRNKNQDRES
CCCCCEEEEEEECCCCCHHHEEEEEEECCCCCCCCHHHHHHCCCCEEEEECCCCCCHHH
RLALGLELRELCRRRSIPFIVNDDLEMAVSLKADGLHLGQGDGDPREARRVLGPGKIIGV
HHHHHHHHHHHHHHCCCCEEECCCCEEEEEEECCCEEECCCCCCHHHHHHHCCCCCEEEE
STHTLSEALEAQAAGVDYIGLGAMFPSRSKEVEHVAGSELLAAIRSSISIPIVAIGGITR
EHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCEEC
DNGASVIDAGADAVAVISAVLSHPDPALAATEIALLFNRRAPFPRGSVLTVAGSDSGGGA
CCCCCEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCC
GIQADLKTVTLLGSYGSSVLTALTAQNTRGVSGIHGVPPAFVADQLDAVFSDIPVDVVKT
CEEECCEEEEEEHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHC
GMLFSAETIVAIAAKLTEYRRRMVVVDPVMVAKGGANLIDRGAVSVLKERLFPLAYLVTP
CCCCCHHHHHHHHHHHHHHHCCEEEECCEEEECCCCCHHHHHHHHHHHHHHCCEEEEECC
NIPEAERLTGANISDEESMREAARRLHRLGARNVLLKGGHLLAGDSVDILFDGAAFHRFV
CCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCEEEECCCEEEEECCHHHHHHC
SPRILSKNTHGTGCTFASAIATYLAQGDPLREAIARAKRYITAAIRLAQPLGRGHGPVNH
CCHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
ILAAEDVRDR
HEECHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA