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The map label for this gene is thiC-1 [H]

Identifier: 39995711

GI number: 39995711

Start: 637928

End: 639238

Strand: Reverse

Name: thiC-1 [H]

Synonym: GSU0604

Alternate gene names: 39995711

Gene position: 639238-637928 (Counterclockwise)

Preceding gene: 39995712

Following gene: 39995710

Centisome position: 16.76

GC content: 63.08

Gene sequence:

>1311_bases
ATGGCTATGACGCAACTGGAATACGCCCGCAACGGAATCATCACCGACAAGATGAAAGAGGCCGCCCTGGCCGAAGGGGT
ATCGCCCGAATTCATCAAGGCGGGCATTGCCGACGGGACCATCATCATCTGCCACAACAACAAGCATCACAACGGCCGCC
CCCTGGCCGTGGGCACAGGGCTACGGACCAAGGTCAACGCAAACATCGGCACCTCCGCCGACGACACGGATATCACCAAG
GAGCTTGAGAAGGCCCGGGTGGCGGTTCGCCACGGTGCCGACGCCATCATGGATCTCTCCACCGGCGGACCGGTGGACGA
AATCCGCCGCGCGATCATCGCCGAGACGAATGCCTGCATCGGCAGCGTCCCCCTCTACCAGGCGGCCCTTGATGCGGTCA
GGACCAAGAAAAAGGCCATCGTCGACATGACCGTGGACGATATCTTCGAGGGGATCATCAAGCATGCCGAGGACGGAGTC
GACTTCATAACCGTCCACTGCGGCGTGACCCGCGCCACGGTCGAGCGCATGAAGAACGAGGGGCGCATCATGGACGTGGT
TTCCCGCGGCGGCGCCTTCACCGTGGAGTGGATGACCTACAACAACGCCGAAAACCCTCTCTTCGAGCACTTTGACCGGC
TGCTCGACATCGTCAAGGCATATGACATGACCCTGTCGCTGGGGGACGGCTTCCGCCCCGGCTGCCTCGCGGATGCCACT
GACCGGGCCCAGATCCACGAGCTGATCCTTCTGGGCGAGCTGACCCAGCGGGCTCAGGACGTCGGGGTTCAGGTCATGAT
CGAAGGCCCCGGGCACGTGCCGCTCAACCAGATCGAAGCGAACATTCTCCTCCAGAAGCGACTCTGCCACGGCGCTCCCT
TTTATGTCCTCGGCCCGCTGGTAACCGATATCGCTCCCGGCTACGACCACATCACCTGCGCGATCGGCGGCGCCATCGCC
GCGGCGGCCGGGGCCGACTTCCTCTGCTACGTGACACCGAGCGAGCACCTGCGACTGCCGAGCGTGGAAGACGTCCGCGA
AGGGGTCATCGCCTCCCGCATCGCCGCCCATGCCGCCGATATAGCCAAAGGGGTAAAGGGGGCCATGGCTAAGGACATCG
CCATGGCTAAATGCCGTAAGAAGCTGGACTGGGAAGGTCAATTCGAACAGGCCCTCGACCCGGAAAAAGCCCGGCGCATG
CGCGACGAATCGGGCGTGGCCGAGCACGGCGCCTGCACCATGTGTGGCGAGTTCTGTGCTTACAAGGTGATGGACGACGC
CATGGAAAAGCAGCGGACGGCAACCCTCTAG

Upstream 100 bases:

>100_bases
CACGGACCGGTCAACCATATCCTCGCCGCGGAGGACGTCAGGGACCGGTGATCGGGGACCGGCAGAAATCACCCGGATAA
CGACCAAGGAGTATCGACAC

Downstream 100 bases:

>100_bases
CGACCGGGATTACTTATTGACACCATCCGCCAGTTAGGTTAAATACTGGTGTCTCTTCATTGCAGACGATGCATTCCATG
GCAGGCCGCCGGCTTCCGAT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase 2; HMP-P synthase 2; HMP-phosphate synthase 2; HMPP synthase 2; Thiamine biosynthesis protein thiC 2 [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MAMTQLEYARNGIITDKMKEAALAEGVSPEFIKAGIADGTIIICHNNKHHNGRPLAVGTGLRTKVNANIGTSADDTDITK
ELEKARVAVRHGADAIMDLSTGGPVDEIRRAIIAETNACIGSVPLYQAALDAVRTKKKAIVDMTVDDIFEGIIKHAEDGV
DFITVHCGVTRATVERMKNEGRIMDVVSRGGAFTVEWMTYNNAENPLFEHFDRLLDIVKAYDMTLSLGDGFRPGCLADAT
DRAQIHELILLGELTQRAQDVGVQVMIEGPGHVPLNQIEANILLQKRLCHGAPFYVLGPLVTDIAPGYDHITCAIGGAIA
AAAGADFLCYVTPSEHLRLPSVEDVREGVIASRIAAHAADIAKGVKGAMAKDIAMAKCRKKLDWEGQFEQALDPEKARRM
RDESGVAEHGACTMCGEFCAYKVMDDAMEKQRTATL

Sequences:

>Translated_436_residues
MAMTQLEYARNGIITDKMKEAALAEGVSPEFIKAGIADGTIIICHNNKHHNGRPLAVGTGLRTKVNANIGTSADDTDITK
ELEKARVAVRHGADAIMDLSTGGPVDEIRRAIIAETNACIGSVPLYQAALDAVRTKKKAIVDMTVDDIFEGIIKHAEDGV
DFITVHCGVTRATVERMKNEGRIMDVVSRGGAFTVEWMTYNNAENPLFEHFDRLLDIVKAYDMTLSLGDGFRPGCLADAT
DRAQIHELILLGELTQRAQDVGVQVMIEGPGHVPLNQIEANILLQKRLCHGAPFYVLGPLVTDIAPGYDHITCAIGGAIA
AAAGADFLCYVTPSEHLRLPSVEDVREGVIASRIAAHAADIAKGVKGAMAKDIAMAKCRKKLDWEGQFEQALDPEKARRM
RDESGVAEHGACTMCGEFCAYKVMDDAMEKQRTATL
>Mature_435_residues
AMTQLEYARNGIITDKMKEAALAEGVSPEFIKAGIADGTIIICHNNKHHNGRPLAVGTGLRTKVNANIGTSADDTDITKE
LEKARVAVRHGADAIMDLSTGGPVDEIRRAIIAETNACIGSVPLYQAALDAVRTKKKAIVDMTVDDIFEGIIKHAEDGVD
FITVHCGVTRATVERMKNEGRIMDVVSRGGAFTVEWMTYNNAENPLFEHFDRLLDIVKAYDMTLSLGDGFRPGCLADATD
RAQIHELILLGELTQRAQDVGVQVMIEGPGHVPLNQIEANILLQKRLCHGAPFYVLGPLVTDIAPGYDHITCAIGGAIAA
AAGADFLCYVTPSEHLRLPSVEDVREGVIASRIAAHAADIAKGVKGAMAKDIAMAKCRKKLDWEGQFEQALDPEKARRMR
DESGVAEHGACTMCGEFCAYKVMDDAMEKQRTATL

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=466, Percent_Identity=43.5622317596566, Blast_Score=367, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 47151; Mature: 47019

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMTQLEYARNGIITDKMKEAALAEGVSPEFIKAGIADGTIIICHNNKHHNGRPLAVGTG
CCCCHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEECCCCCCCCCEEEECC
LRTKVNANIGTSADDTDITKELEKARVAVRHGADAIMDLSTGGPVDEIRRAIIAETNACI
CCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCCCC
GSVPLYQAALDAVRTKKKAIVDMTVDDIFEGIIKHAEDGVDFITVHCGVTRATVERMKNE
CCCHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCC
GRIMDVVSRGGAFTVEWMTYNNAENPLFEHFDRLLDIVKAYDMTLSLGDGFRPGCLADAT
CCEEEHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCC
DRAQIHELILLGELTQRAQDVGVQVMIEGPGHVPLNQIEANILLQKRLCHGAPFYVLGPL
CHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
VTDIAPGYDHITCAIGGAIAAAAGADFLCYVTPSEHLRLPSVEDVREGVIASRIAAHAAD
HHHCCCCCCEEEEHHHHHHHHHCCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHH
IAKGVKGAMAKDIAMAKCRKKLDWEGQFEQALDPEKARRMRDESGVAEHGACTMCGEFCA
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHH
YKVMDDAMEKQRTATL
HHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AMTQLEYARNGIITDKMKEAALAEGVSPEFIKAGIADGTIIICHNNKHHNGRPLAVGTG
CCCHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEECCCCCCCCCEEEECC
LRTKVNANIGTSADDTDITKELEKARVAVRHGADAIMDLSTGGPVDEIRRAIIAETNACI
CCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCCCC
GSVPLYQAALDAVRTKKKAIVDMTVDDIFEGIIKHAEDGVDFITVHCGVTRATVERMKNE
CCCHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCC
GRIMDVVSRGGAFTVEWMTYNNAENPLFEHFDRLLDIVKAYDMTLSLGDGFRPGCLADAT
CCEEEHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCC
DRAQIHELILLGELTQRAQDVGVQVMIEGPGHVPLNQIEANILLQKRLCHGAPFYVLGPL
CHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
VTDIAPGYDHITCAIGGAIAAAAGADFLCYVTPSEHLRLPSVEDVREGVIASRIAAHAAD
HHHCCCCCCEEEEHHHHHHHHHCCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHH
IAKGVKGAMAKDIAMAKCRKKLDWEGQFEQALDPEKARRMRDESGVAEHGACTMCGEFCA
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHH
YKVMDDAMEKQRTATL
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA