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The map label for this gene is glyS

Identifier: 39995686

GI number: 39995686

Start: 608194

End: 610257

Strand: Direct

Name: glyS

Synonym: GSU0579

Alternate gene names: 39995686

Gene position: 608194-610257 (Clockwise)

Preceding gene: 39995685

Following gene: 39995687

Centisome position: 15.95

GC content: 66.28

Gene sequence:

>2064_bases
ATGAGCAAGGAACTGTTTCTCGAAATAGGCACCGAGGAGATCCCCGCCGGCTTCCTGCCCAAGGCCATGGCCGACATGGA
GGCCATCGTCACGAAGGAGCTGGAGAACGCCCGCCTTGCCTTCGGCGAGGTGAAGACCTTCGCCACCCCCCGTCGTCTGG
CGCTGGTGGTGAAGGGGCTGCCGACAGTTCAGCCCGATGCCGAGATTACCGCCCTGGGGCCGGCCAGGAACATCGCCTTC
GGTCCCGACGGCACGCCGTCCAAGGCGGCCGAGGGCTTCGCCCGTGGCCAGGGGGTCGACGTGGCGTCCCTGACCCTGGT
CTCCACCGAGAAGGGCGAATACGTGGCCGCTGTCCGCAAGGAGAGCGGCCGGCCGGTACCGGACCTCCTCGCCGAGATCC
TGCCGCGGCTGGTGGGGGGCATCCCGTTCCGCAAGTCCATGCGCTGGGCTGATCTGGACGTGCGTTTCGCCCGCCCGATC
CACTGGATCGTGGCCCTGTTCGACGGGGTCGTCGTCCCCTTCGCCTTCGGCAACGTCCAGAGCGGCAATGTCTCGCGGGG
GCACCGGTTCATGGCCAACCAGCCGTTCCCGGTCCGTGACTTCGCCCACTACCTGGACGAGTGCGAGCGGCACTTCGTGA
TCCCCGATCCCGAGCGGCGAAAGGAAACCATCCGCCGGGAGATCCACCGGGTTGCCAAGACCGCCGGTGGCCACCTCCTC
CCCGACGAGGGACTCCTGGACGAGGTTGCTTACCTGGTGGAGTACCCCAGCGTGGTCCACGGCACCTTCTCGCCCGATTT
TCTCAAGGTTCCCCGCGAGGTGCTCATCACCTCCATGCGGAGCCACCAGCGCTACTTCTCCATCGTCGACGACGCCGGCA
AGCTCATGCCCGGTTTCATCACCATCAACAATACCCTGACCGAGGACCCCTCCGTGGTGGTCAAGGGGAACGAGCGCGTC
CTGCGCGCCCGCCTCTCCGACGCCCGCTTCTTCTTCGAGGAAGATCAGAAGGTGAAGCTGGAGACCCGGGTGGAGTCCTT
GAAAAACGTGGTCTACCAGCAGAAGCTCGGCACCTCCTACGAGAAGATGGAGCGCTTTCGCGCCCTGGCCGAGGGGCTGG
CCGACCTCCTCAATCCGGCGGTTAAGGCCAAGACATCCCGGGCCGCCTTCCTCTGCAAGGCCGACCTGGTTTCGGGTATG
GTCGGTGAGTTCCCCGAGGTACAGGGGATCATGGGCCGTGAGTATGCCCTGCTCCAAGGCGAAGACGCCGAAGTTGCCGC
CGCCATCGCCGAGCACTACCTCCCCACCCAGGCCGGTGGGGACCTCCCCGCATCCGACATCGGCGCCTTCGTCTCCATCG
CCGACAAGCTCGATACCATCTGCGGCTGCTTCGGGGTGGGGCTGATCCCCACCGGTTCGGCCGATCCCTATGCCCTGCGC
CGTTCGGCCCTGGGCATCATCAACATCATTCTCGACCGGGGCTGCCGCCTCTCGCTGGAAGGAGAGATCGGGAAGGCCCT
GGAACTCCTCTCCGCCAAACTGACCCGCCCCGCCGCCGAGGTGATGGCTGATGTGCTGGAATTCTTCCGGGGCCGGTTCG
TGAACCTCATGGCCGACCGCTATCCGGCCGATGCCGTGGACGCGGCCATTGCCGCCGGCTTCGACGACCTGGTGGACGCC
GAGGCCCGCATCGGCGCACTGGCGGCCTTCAAGGGGCGCCCGGATTTCGACTCCCTGGCAGTGGCCTTCAAACGGGTCTG
CAACATCGTCAAGGACGGGGTCGATCAGCCCGTGGATGCCGCCCTGTTCCAGGAGCCGGCCGAGGGTGCCCTGTTCGCGG
CGTTCCAGCAGGTCCGCGCCGACGTGGAGGCCCGCACCGCATCCGGCGATTACCTGGCTGCGCTCACCGGTATTGCCGCT
CTCAAGGGCGCCGTGGACGACTTCTTCGACAAGGTGATGGTTATGGCCGAGGACGAACGGGTCCGAACAAACCGGTTAGC
ATTGCTGACCGGGATCGCCCGGCTCTTCGGAGGCGTCGCGGACTTCGCGAAGATAGCCGCCTAG

Upstream 100 bases:

>100_bases
ATCGGCCGGGTCCGCAATGTGGCCCGTCTCTGCGCCGAAGGCTACCTGAAGCTCCGCGAAAGCCTGGGCTTCCCGCTGCT
GAAAGGAGGACGGAAGTAAC

Downstream 100 bases:

>100_bases
CTCATGCTAGGCGGTTTTTTTATCGTTTTAATTTCCCTAAATACCGCTTGAGCAGCATTAAAAAAAATTTATCGCAGCTG
AACGGGTTTGACCGTCAGTA

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 687; Mature: 686

Protein sequence:

>687_residues
MSKELFLEIGTEEIPAGFLPKAMADMEAIVTKELENARLAFGEVKTFATPRRLALVVKGLPTVQPDAEITALGPARNIAF
GPDGTPSKAAEGFARGQGVDVASLTLVSTEKGEYVAAVRKESGRPVPDLLAEILPRLVGGIPFRKSMRWADLDVRFARPI
HWIVALFDGVVVPFAFGNVQSGNVSRGHRFMANQPFPVRDFAHYLDECERHFVIPDPERRKETIRREIHRVAKTAGGHLL
PDEGLLDEVAYLVEYPSVVHGTFSPDFLKVPREVLITSMRSHQRYFSIVDDAGKLMPGFITINNTLTEDPSVVVKGNERV
LRARLSDARFFFEEDQKVKLETRVESLKNVVYQQKLGTSYEKMERFRALAEGLADLLNPAVKAKTSRAAFLCKADLVSGM
VGEFPEVQGIMGREYALLQGEDAEVAAAIAEHYLPTQAGGDLPASDIGAFVSIADKLDTICGCFGVGLIPTGSADPYALR
RSALGIINIILDRGCRLSLEGEIGKALELLSAKLTRPAAEVMADVLEFFRGRFVNLMADRYPADAVDAAIAAGFDDLVDA
EARIGALAAFKGRPDFDSLAVAFKRVCNIVKDGVDQPVDAALFQEPAEGALFAAFQQVRADVEARTASGDYLAALTGIAA
LKGAVDDFFDKVMVMAEDERVRTNRLALLTGIARLFGGVADFAKIAA

Sequences:

>Translated_687_residues
MSKELFLEIGTEEIPAGFLPKAMADMEAIVTKELENARLAFGEVKTFATPRRLALVVKGLPTVQPDAEITALGPARNIAF
GPDGTPSKAAEGFARGQGVDVASLTLVSTEKGEYVAAVRKESGRPVPDLLAEILPRLVGGIPFRKSMRWADLDVRFARPI
HWIVALFDGVVVPFAFGNVQSGNVSRGHRFMANQPFPVRDFAHYLDECERHFVIPDPERRKETIRREIHRVAKTAGGHLL
PDEGLLDEVAYLVEYPSVVHGTFSPDFLKVPREVLITSMRSHQRYFSIVDDAGKLMPGFITINNTLTEDPSVVVKGNERV
LRARLSDARFFFEEDQKVKLETRVESLKNVVYQQKLGTSYEKMERFRALAEGLADLLNPAVKAKTSRAAFLCKADLVSGM
VGEFPEVQGIMGREYALLQGEDAEVAAAIAEHYLPTQAGGDLPASDIGAFVSIADKLDTICGCFGVGLIPTGSADPYALR
RSALGIINIILDRGCRLSLEGEIGKALELLSAKLTRPAAEVMADVLEFFRGRFVNLMADRYPADAVDAAIAAGFDDLVDA
EARIGALAAFKGRPDFDSLAVAFKRVCNIVKDGVDQPVDAALFQEPAEGALFAAFQQVRADVEARTASGDYLAALTGIAA
LKGAVDDFFDKVMVMAEDERVRTNRLALLTGIARLFGGVADFAKIAA
>Mature_686_residues
SKELFLEIGTEEIPAGFLPKAMADMEAIVTKELENARLAFGEVKTFATPRRLALVVKGLPTVQPDAEITALGPARNIAFG
PDGTPSKAAEGFARGQGVDVASLTLVSTEKGEYVAAVRKESGRPVPDLLAEILPRLVGGIPFRKSMRWADLDVRFARPIH
WIVALFDGVVVPFAFGNVQSGNVSRGHRFMANQPFPVRDFAHYLDECERHFVIPDPERRKETIRREIHRVAKTAGGHLLP
DEGLLDEVAYLVEYPSVVHGTFSPDFLKVPREVLITSMRSHQRYFSIVDDAGKLMPGFITINNTLTEDPSVVVKGNERVL
RARLSDARFFFEEDQKVKLETRVESLKNVVYQQKLGTSYEKMERFRALAEGLADLLNPAVKAKTSRAAFLCKADLVSGMV
GEFPEVQGIMGREYALLQGEDAEVAAAIAEHYLPTQAGGDLPASDIGAFVSIADKLDTICGCFGVGLIPTGSADPYALRR
SALGIINIILDRGCRLSLEGEIGKALELLSAKLTRPAAEVMADVLEFFRGRFVNLMADRYPADAVDAAIAAGFDDLVDAE
ARIGALAAFKGRPDFDSLAVAFKRVCNIVKDGVDQPVDAALFQEPAEGALFAAFQQVRADVEARTASGDYLAALTGIAAL
KGAVDDFFDKVMVMAEDERVRTNRLALLTGIARLFGGVADFAKIAA

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=689, Percent_Identity=40.7837445573295, Blast_Score=468, Evalue=1e-133,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_GEOSL (Q74FM7)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_951637.1
- ProteinModelPortal:   Q74FM7
- GeneID:   2685658
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU0579
- NMPDR:   fig|243231.1.peg.575
- TIGR:   GSU0579
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- ProtClustDB:   PRK01233
- BioCyc:   GSUL243231:GSU_0579-MONOMER
- BRENDA:   6.1.1.14
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 74632; Mature: 74500

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKELFLEIGTEEIPAGFLPKAMADMEAIVTKELENARLAFGEVKTFATPRRLALVVKGL
CCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
PTVQPDAEITALGPARNIAFGPDGTPSKAAEGFARGQGVDVASLTLVSTEKGEYVAAVRK
CCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCEEEHEEC
ESGRPVPDLLAEILPRLVGGIPFRKSMRWADLDVRFARPIHWIVALFDGVVVPFAFGNVQ
CCCCCHHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCC
SGNVSRGHRFMANQPFPVRDFAHYLDECERHFVIPDPERRKETIRREIHRVAKTAGGHLL
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
PDEGLLDEVAYLVEYPSVVHGTFSPDFLKVPREVLITSMRSHQRYFSIVDDAGKLMPGFI
CCCCHHHHHHHHHHCCHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
TINNTLTEDPSVVVKGNERVLRARLSDARFFFEEDQKVKLETRVESLKNVVYQQKLGTSY
EECCCCCCCCCEEEECCHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHCCCH
EKMERFRALAEGLADLLNPAVKAKTSRAAFLCKADLVSGMVGEFPEVQGIMGREYALLQG
HHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCEEEECC
EDAEVAAAIAEHYLPTQAGGDLPASDIGAFVSIADKLDTICGCFGVGLIPTGSADPYALR
CCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
RSALGIINIILDRGCRLSLEGEIGKALELLSAKLTRPAAEVMADVLEFFRGRFVNLMADR
HHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
YPADAVDAAIAAGFDDLVDAEARIGALAAFKGRPDFDSLAVAFKRVCNIVKDGVDQPVDA
CCHHHHHHHHHCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHH
ALFQEPAEGALFAAFQQVRADVEARTASGDYLAALTGIAALKGAVDDFFDKVMVMAEDER
HHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
VRTNRLALLTGIARLFGGVADFAKIAA
HHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKELFLEIGTEEIPAGFLPKAMADMEAIVTKELENARLAFGEVKTFATPRRLALVVKGL
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
PTVQPDAEITALGPARNIAFGPDGTPSKAAEGFARGQGVDVASLTLVSTEKGEYVAAVRK
CCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCEEEHEEC
ESGRPVPDLLAEILPRLVGGIPFRKSMRWADLDVRFARPIHWIVALFDGVVVPFAFGNVQ
CCCCCHHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCC
SGNVSRGHRFMANQPFPVRDFAHYLDECERHFVIPDPERRKETIRREIHRVAKTAGGHLL
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
PDEGLLDEVAYLVEYPSVVHGTFSPDFLKVPREVLITSMRSHQRYFSIVDDAGKLMPGFI
CCCCHHHHHHHHHHCCHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
TINNTLTEDPSVVVKGNERVLRARLSDARFFFEEDQKVKLETRVESLKNVVYQQKLGTSY
EECCCCCCCCCEEEECCHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHCCCH
EKMERFRALAEGLADLLNPAVKAKTSRAAFLCKADLVSGMVGEFPEVQGIMGREYALLQG
HHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCEEEECC
EDAEVAAAIAEHYLPTQAGGDLPASDIGAFVSIADKLDTICGCFGVGLIPTGSADPYALR
CCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
RSALGIINIILDRGCRLSLEGEIGKALELLSAKLTRPAAEVMADVLEFFRGRFVNLMADR
HHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
YPADAVDAAIAAGFDDLVDAEARIGALAAFKGRPDFDSLAVAFKRVCNIVKDGVDQPVDA
CCHHHHHHHHHCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHH
ALFQEPAEGALFAAFQQVRADVEARTASGDYLAALTGIAALKGAVDDFFDKVMVMAEDER
HHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
VRTNRLALLTGIARLFGGVADFAKIAA
HHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA