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The map label for this gene is ppdK [H]

Identifier: 39995687

GI number: 39995687

Start: 610378

End: 613044

Strand: Direct

Name: ppdK [H]

Synonym: GSU0580

Alternate gene names: 39995687

Gene position: 610378-613044 (Clockwise)

Preceding gene: 39995686

Following gene: 39995688

Centisome position: 16.0

GC content: 63.33

Gene sequence:

>2667_bases
ATGGCTGGCAAGTATGTTTACTTCTTCGGCAATGGCCAGGCCGAAGGAAAGGCCGAGATGAAGAATCTGCTGGGGGGCAA
GGGTGCCAACCTGGCGGAGATGACAGCGATCGGGCTCCCGGTTCCGCCGGGGTTCACCATCACCACCGAGGTCTGCACCT
ACTACTACGCCAACAACCGGAGCTATCCGCCGACCCTGGCGGCCGAGGTTGCCGATAACCTGAAGAAGGTGGAGGCCCTC
ATGGGGCGCACCTTCGGCGACCGGAACAACCCCCTGCTGGTGTCGGTCCGTTCCGGCGCCCGGGCTTCCATGCCGGGTAT
GATGGATACCATCCTTAACCTGGGACTCAACGATGAGACAGTCCAGGGAATCATCGCCCAGAGCGGAGACGAGCGTTTCG
CCTACGATGCCTACCGCCGCTTCGTGCAGATGTATTCCGATGTGGTCATGGGCATGGACAAGGACCTGCTCGAACACCTC
TTGGAGCAGAAGAAGGAGGAGAAAGGGGTCCATCTGGACACGGATCTCACCGCCGCCGACTGGAAGGAACTGGTCGGCAA
GTTCAAGGCCAAGATCCGCGAGACCCTCGGCAAGGAGTTCCCCGAAGATCCGCAGGAGCAGCTCTGGGGCGCCGTGGGTG
CCGTGTTCGGCTCCTGGATGAACCAGCGGGCCATCACCTATCGCCGGCTCAATAACATTCCCGCCGACTGGGGCACCGCA
GTCAACGTTCAGTCCATGGTTTACGGCAACATGGGGAACGACTGCGCCACCGGCGTTGCCTTTACCCGCGACCCCTCCAC
TGGTGAAAACTATTTCTACGGCGAGTACCTGGTGAACGCCCAGGGTGAGGACGTGGTGGCCGGCATCCGAACCCCGCAGC
CCATCAACCGGGCCAACAGCAAGGACACCACCCTCCCGGCCATGGAAGACGTGCTGCCCGAGTGCTATCAGCAGCTCGTC
CAGATCCGCGGCATCCTTGAGAAGCACTACAGGGACATGCAGGACATCGAGTTCACCATCGAGAAGGGCAAGCTGTTCAT
GCTCCAGACCCGCAACGGCAAGCGGACCGCCAAGGCGGCCATCAAGATCGCCGTGGACATGGTGCGGGAGGGGCTCATCG
ACGAAAAGACCGCTGTGCTGCGGGTGTCTCCGTCCCAGCTTGACCAGCTCCTCCATCCGTCCCTTGATCCCAAGGCTCAG
AAAAGGGTCATTGCCAAGGGCCTTCCTGCCTCCCCCGGAGCGGCCAGCGGCGAGGTTGTCTTCACCGCGGACGAGGCTGA
GGCTGCCGCCCGCCTGGGGCTCAAGGTGATCCTAGTTCGGGTCGAGACGAGCCCCGAGGATATTCACGGCATGCACGCGG
CCCAGGGGATCCTCACCGCCCGGGGCGGCATGACCTCCCACGCGGCGGTGGTCGCAAGGGGCATGGGCAAGTGCTGCGTC
GCCGGCTGCGGCGACATCAAGGTCGATTACGCCGGGAGCCAGTTCGCCACCGCCAAGGGGCAGGTGGTCAAGAAGGGGGA
TGTCATCACCCTGGACGGCTCCACCGGCGAGGTGATGCTCGGCGAGGTGCCGACAGTTCCGCCGCAGCTTACCGGGGACT
TCGGCACCCTGATGGAGTGGGTGGACCGCTTCCGCAAGTTGAAAGTGCGAACTAATGCCGACACCCCCAATGATTCGCGG
GTGGCCCGCGAGTTCGGCGCCGAGGGGATCGGCCTCTGCCGCACCGAGCACATGTTCTTCGAGGCCGACCGGATTGCCGC
CGTGCGCGAGATGATTCTCGCCGAGGACGTTGAGGGGCGCAAGAAGGCCCTTGCCAAGATTCTGCCCATGCAGAAGGGTG
ACTTCGTCGGCATCTTCAGGGAGATGAAAGGACTGCCCGTCACCATTCGCCTGCTGGATCCGCCGCTCCACGAGTTCCTT
CCCCACGAGGACAAGGACATCGATGCCCTGGCCAAGACCATGGGGGTAACGCCTCAATCGCTCAGGGCCAAGGTGGATTA
CCTCCACGAGTTCAACCCGATGCTCGGCCACCGCGGCTGCCGGCTTGGGCTCACCTTCCCGGAGATTTACGACATGCAGG
TGCAGGCCATCATGGAAGCCGCCTGCGAACTGACCAAGAACGAAGGGTTCAGCATCGTGCCCGAGATCATGATCCCGCTG
GTGGGGGTGGTGACCGAGCTTGCCCGCCTGCGGGAGAACACGGTGCGCGTGTGCGAGGAGGTCGTCGCCGCCTACGGCGT
CAAGGTGGAGTACCTGATCGGCACCATGATCGAGCTCCCCCGTGCCGCCCTCACCGCCGATGAGATTGCCCGGGAGGCCG
AATTCTTCTCCTTCGGCACCAACGACCTGACCCAGACCACGTTCGGCCTTTCCCGCGACGATGCCGGCAAGTTCCTGCCG
TTCTATGTGGAAACCGGTTTGCTGGAAGACGACCCCTTTGTCTCCCTTGACCAGAACGGGGTCGGTCTTCTTGTGAAGAT
GGCAGTGGAAAAAGGGCGCGCCACCCGCCCCGGCATCAAGCTCGGCATTTGCGGCGAGCATGGCGGCGATCCGTCCTCGG
TCATCTTCTGCCACCAGATCGGTCTCGACTATGTATCCTGCTCGCCGTTCCGGGTGCCCATCGCCCGGCTCGCGGCGGCC
CATGCCGCCCTGGAGGAAGGGAAGTAG

Upstream 100 bases:

>100_bases
TATCGTTTTAATTTCCCTAAATACCGCTTGAGCAGCATTAAAAAAAATTTATCGCAGCTGAACGGGTTTGACCGTCAGTA
AAAACTCAAGGAGGCGTGCA

Downstream 100 bases:

>100_bases
CAGAGTGCATCAGCGGGGGGGACAGGGTCTCCCCCGCTTTTTTTCGGCCAAATATCAGCTTGACAGGCCGTGTGTATTGG
TGCTAGAAAGCGAAAAATTC

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 888; Mature: 887

Protein sequence:

>888_residues
MAGKYVYFFGNGQAEGKAEMKNLLGGKGANLAEMTAIGLPVPPGFTITTEVCTYYYANNRSYPPTLAAEVADNLKKVEAL
MGRTFGDRNNPLLVSVRSGARASMPGMMDTILNLGLNDETVQGIIAQSGDERFAYDAYRRFVQMYSDVVMGMDKDLLEHL
LEQKKEEKGVHLDTDLTAADWKELVGKFKAKIRETLGKEFPEDPQEQLWGAVGAVFGSWMNQRAITYRRLNNIPADWGTA
VNVQSMVYGNMGNDCATGVAFTRDPSTGENYFYGEYLVNAQGEDVVAGIRTPQPINRANSKDTTLPAMEDVLPECYQQLV
QIRGILEKHYRDMQDIEFTIEKGKLFMLQTRNGKRTAKAAIKIAVDMVREGLIDEKTAVLRVSPSQLDQLLHPSLDPKAQ
KRVIAKGLPASPGAASGEVVFTADEAEAAARLGLKVILVRVETSPEDIHGMHAAQGILTARGGMTSHAAVVARGMGKCCV
AGCGDIKVDYAGSQFATAKGQVVKKGDVITLDGSTGEVMLGEVPTVPPQLTGDFGTLMEWVDRFRKLKVRTNADTPNDSR
VAREFGAEGIGLCRTEHMFFEADRIAAVREMILAEDVEGRKKALAKILPMQKGDFVGIFREMKGLPVTIRLLDPPLHEFL
PHEDKDIDALAKTMGVTPQSLRAKVDYLHEFNPMLGHRGCRLGLTFPEIYDMQVQAIMEAACELTKNEGFSIVPEIMIPL
VGVVTELARLRENTVRVCEEVVAAYGVKVEYLIGTMIELPRAALTADEIAREAEFFSFGTNDLTQTTFGLSRDDAGKFLP
FYVETGLLEDDPFVSLDQNGVGLLVKMAVEKGRATRPGIKLGICGEHGGDPSSVIFCHQIGLDYVSCSPFRVPIARLAAA
HAALEEGK

Sequences:

>Translated_888_residues
MAGKYVYFFGNGQAEGKAEMKNLLGGKGANLAEMTAIGLPVPPGFTITTEVCTYYYANNRSYPPTLAAEVADNLKKVEAL
MGRTFGDRNNPLLVSVRSGARASMPGMMDTILNLGLNDETVQGIIAQSGDERFAYDAYRRFVQMYSDVVMGMDKDLLEHL
LEQKKEEKGVHLDTDLTAADWKELVGKFKAKIRETLGKEFPEDPQEQLWGAVGAVFGSWMNQRAITYRRLNNIPADWGTA
VNVQSMVYGNMGNDCATGVAFTRDPSTGENYFYGEYLVNAQGEDVVAGIRTPQPINRANSKDTTLPAMEDVLPECYQQLV
QIRGILEKHYRDMQDIEFTIEKGKLFMLQTRNGKRTAKAAIKIAVDMVREGLIDEKTAVLRVSPSQLDQLLHPSLDPKAQ
KRVIAKGLPASPGAASGEVVFTADEAEAAARLGLKVILVRVETSPEDIHGMHAAQGILTARGGMTSHAAVVARGMGKCCV
AGCGDIKVDYAGSQFATAKGQVVKKGDVITLDGSTGEVMLGEVPTVPPQLTGDFGTLMEWVDRFRKLKVRTNADTPNDSR
VAREFGAEGIGLCRTEHMFFEADRIAAVREMILAEDVEGRKKALAKILPMQKGDFVGIFREMKGLPVTIRLLDPPLHEFL
PHEDKDIDALAKTMGVTPQSLRAKVDYLHEFNPMLGHRGCRLGLTFPEIYDMQVQAIMEAACELTKNEGFSIVPEIMIPL
VGVVTELARLRENTVRVCEEVVAAYGVKVEYLIGTMIELPRAALTADEIAREAEFFSFGTNDLTQTTFGLSRDDAGKFLP
FYVETGLLEDDPFVSLDQNGVGLLVKMAVEKGRATRPGIKLGICGEHGGDPSSVIFCHQIGLDYVSCSPFRVPIARLAAA
HAALEEGK
>Mature_887_residues
AGKYVYFFGNGQAEGKAEMKNLLGGKGANLAEMTAIGLPVPPGFTITTEVCTYYYANNRSYPPTLAAEVADNLKKVEALM
GRTFGDRNNPLLVSVRSGARASMPGMMDTILNLGLNDETVQGIIAQSGDERFAYDAYRRFVQMYSDVVMGMDKDLLEHLL
EQKKEEKGVHLDTDLTAADWKELVGKFKAKIRETLGKEFPEDPQEQLWGAVGAVFGSWMNQRAITYRRLNNIPADWGTAV
NVQSMVYGNMGNDCATGVAFTRDPSTGENYFYGEYLVNAQGEDVVAGIRTPQPINRANSKDTTLPAMEDVLPECYQQLVQ
IRGILEKHYRDMQDIEFTIEKGKLFMLQTRNGKRTAKAAIKIAVDMVREGLIDEKTAVLRVSPSQLDQLLHPSLDPKAQK
RVIAKGLPASPGAASGEVVFTADEAEAAARLGLKVILVRVETSPEDIHGMHAAQGILTARGGMTSHAAVVARGMGKCCVA
GCGDIKVDYAGSQFATAKGQVVKKGDVITLDGSTGEVMLGEVPTVPPQLTGDFGTLMEWVDRFRKLKVRTNADTPNDSRV
AREFGAEGIGLCRTEHMFFEADRIAAVREMILAEDVEGRKKALAKILPMQKGDFVGIFREMKGLPVTIRLLDPPLHEFLP
HEDKDIDALAKTMGVTPQSLRAKVDYLHEFNPMLGHRGCRLGLTFPEIYDMQVQAIMEAACELTKNEGFSIVPEIMIPLV
GVVTELARLRENTVRVCEEVVAAYGVKVEYLIGTMIELPRAALTADEIAREAEFFSFGTNDLTQTTFGLSRDDAGKFLPF
YVETGLLEDDPFVSLDQNGVGLLVKMAVEKGRATRPGIKLGICGEHGGDPSSVIFCHQIGLDYVSCSPFRVPIARLAAAH
AALEEGK

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=473, Percent_Identity=25.7928118393235, Blast_Score=108, Evalue=2e-24,
Organism=Escherichia coli, GI1787994, Length=698, Percent_Identity=25.214899713467, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789193, Length=427, Percent_Identity=25.5269320843091, Blast_Score=87, Evalue=5e-18,
Organism=Escherichia coli, GI1788726, Length=334, Percent_Identity=24.5508982035928, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI48994992, Length=439, Percent_Identity=23.6902050113895, Blast_Score=74, Evalue=5e-14,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 97113; Mature: 96982

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGKYVYFFGNGQAEGKAEMKNLLGGKGANLAEMTAIGLPVPPGFTITTEVCTYYYANNR
CCCCEEEEEECCCCCCHHHHHHHHCCCCCCHHHHHEECCCCCCCCEEHHHHHEEEEECCC
SYPPTLAAEVADNLKKVEALMGRTFGDRNNPLLVSVRSGARASMPGMMDTILNLGLNDET
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCHHH
VQGIIAQSGDERFAYDAYRRFVQMYSDVVMGMDKDLLEHLLEQKKEEKGVHLDTDLTAAD
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEECCCCCHHH
WKELVGKFKAKIRETLGKEFPEDPQEQLWGAVGAVFGSWMNQRAITYRRLNNIPADWGTA
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
VNVQSMVYGNMGNDCATGVAFTRDPSTGENYFYGEYLVNAQGEDVVAGIRTPQPINRANS
EEEHHHHCCCCCCCHHCCCEEECCCCCCCCEEEEEEEECCCCCCEEEECCCCCCCCCCCC
KDTTLPAMEDVLPECYQQLVQIRGILEKHYRDMQDIEFTIEKGKLFMLQTRNGKRTAKAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEECCEEEEEEECCCCHHHHHH
IKIAVDMVREGLIDEKTAVLRVSPSQLDQLLHPSLDPKAQKRVIAKGLPASPGAASGEVV
HHHHHHHHHHCCCCCCCEEEEECHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCEE
FTADEAEAAARLGLKVILVRVETSPEDIHGMHAAQGILTARGGMTSHAAVVARGMGKCCV
EEECHHHHHHHCCEEEEEEEEECCCHHHHHHHHHHHHHEECCCCCHHHHHHHHCCCCCEE
AGCGDIKVDYAGSQFATAKGQVVKKGDVITLDGSTGEVMLGEVPTVPPQLTGDFGTLMEW
CCCCCEEEECCCCCEECCCCCEEECCCEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHH
VDRFRKLKVRTNADTPNDSRVAREFGAEGIGLCRTEHMFFEADRIAAVREMILAEDVEGR
HHHHHHEEEEECCCCCCHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCHH
KKALAKILPMQKGDFVGIFREMKGLPVTIRLLDPPLHEFLPHEDKDIDALAKTMGVTPQS
HHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCCHHHHHHHHCCCHHH
LRAKVDYLHEFNPMLGHRGCRLGLTFPEIYDMQVQAIMEAACELTKNEGFSIVPEIMIPL
HHHHHHHHHHCCHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHH
VGVVTELARLRENTVRVCEEVVAAYGVKVEYLIGTMIELPRAALTADEIAREAEFFSFGT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
NDLTQTTFGLSRDDAGKFLPFYVETGLLEDDPFVSLDQNGVGLLVKMAVEKGRATRPGIK
CCHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCEEECCCCCEEEEEEHHHCCCCCCCCEE
LGICGEHGGDPSSVIFCHQIGLDYVSCSPFRVPIARLAAAHAALEEGK
EEECCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AGKYVYFFGNGQAEGKAEMKNLLGGKGANLAEMTAIGLPVPPGFTITTEVCTYYYANNR
CCCEEEEEECCCCCCHHHHHHHHCCCCCCHHHHHEECCCCCCCCEEHHHHHEEEEECCC
SYPPTLAAEVADNLKKVEALMGRTFGDRNNPLLVSVRSGARASMPGMMDTILNLGLNDET
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCHHH
VQGIIAQSGDERFAYDAYRRFVQMYSDVVMGMDKDLLEHLLEQKKEEKGVHLDTDLTAAD
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEECCCCCHHH
WKELVGKFKAKIRETLGKEFPEDPQEQLWGAVGAVFGSWMNQRAITYRRLNNIPADWGTA
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
VNVQSMVYGNMGNDCATGVAFTRDPSTGENYFYGEYLVNAQGEDVVAGIRTPQPINRANS
EEEHHHHCCCCCCCHHCCCEEECCCCCCCCEEEEEEEECCCCCCEEEECCCCCCCCCCCC
KDTTLPAMEDVLPECYQQLVQIRGILEKHYRDMQDIEFTIEKGKLFMLQTRNGKRTAKAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEECCEEEEEEECCCCHHHHHH
IKIAVDMVREGLIDEKTAVLRVSPSQLDQLLHPSLDPKAQKRVIAKGLPASPGAASGEVV
HHHHHHHHHHCCCCCCCEEEEECHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCEE
FTADEAEAAARLGLKVILVRVETSPEDIHGMHAAQGILTARGGMTSHAAVVARGMGKCCV
EEECHHHHHHHCCEEEEEEEEECCCHHHHHHHHHHHHHEECCCCCHHHHHHHHCCCCCEE
AGCGDIKVDYAGSQFATAKGQVVKKGDVITLDGSTGEVMLGEVPTVPPQLTGDFGTLMEW
CCCCCEEEECCCCCEECCCCCEEECCCEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHH
VDRFRKLKVRTNADTPNDSRVAREFGAEGIGLCRTEHMFFEADRIAAVREMILAEDVEGR
HHHHHHEEEEECCCCCCHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCHH
KKALAKILPMQKGDFVGIFREMKGLPVTIRLLDPPLHEFLPHEDKDIDALAKTMGVTPQS
HHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCCHHHHHHHHCCCHHH
LRAKVDYLHEFNPMLGHRGCRLGLTFPEIYDMQVQAIMEAACELTKNEGFSIVPEIMIPL
HHHHHHHHHHCCHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHH
VGVVTELARLRENTVRVCEEVVAAYGVKVEYLIGTMIELPRAALTADEIAREAEFFSFGT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
NDLTQTTFGLSRDDAGKFLPFYVETGLLEDDPFVSLDQNGVGLLVKMAVEKGRATRPGIK
CCHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCEEECCCCCEEEEEEHHHCCCCCCCCEE
LGICGEHGGDPSSVIFCHQIGLDYVSCSPFRVPIARLAAAHAALEEGK
EEECCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 9168612 [H]