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The map label for this gene is ycdT [H]

Identifier: 39995649

GI number: 39995649

Start: 575439

End: 576464

Strand: Direct

Name: ycdT [H]

Synonym: GSU0542

Alternate gene names: 39995649

Gene position: 575439-576464 (Clockwise)

Preceding gene: 39995648

Following gene: 39995652

Centisome position: 15.09

GC content: 64.42

Gene sequence:

>1026_bases
ATGTCCGGCGACATTCTGAACGACATCGTTGCAGCATGCCTGGAGCTTGAACGCAAGGCCTCATCGGTATTCAAGATGTT
CGCGGCACACGCCGGCAGCGACGAGGCGCGGCGTTTCTGGGAGACCGTTGCCGACGAAACCCGTCATCACAGTGCCGTGT
ATGAGCGCCTGCAGGAGAGGGGCGGGCGCGAGAACCTGCCGATCATCATCTACAAGCCGGCCGAGACCCTGGAAGAACTG
GAAATGATCGGCAAGAGCATCGACGAGCAGGTCGAGCGCTATACCGAGGCCCCCTCCAGCGAGGCCGCCTGTCTGCTCGG
CTTCCGCCTGCAGCTCTACCTCCTCCATCCCGCCTTTGCGAGTCTCTGTCGCCTGACCAGGGATGCCAGCGGCGAGGACC
TGCCCGATATCGGCTACGGCCGCTACCTGCGTCGTTTTATCGACGGCATCGGCTCCTGCGGCCTGGCCACCGCCGAAACG
GAACTGCTGGGCGAGGCCCTGTTCCGGCTCTGGAACGAGGCGCGGCAGCTGGCAGCCCAGTCCCACTTCGACGCCCTCAC
GGGGGTCATGACACGGGCCGGCTTCTTCAAGACCGTGGGCTCACTCGCCTACGCGGCCCAGCGCAGCGGAAGCAACGTGG
GCATCATGCTCATTGATCTGGATTATTTCAAACTGGTGGGGGACAACTACGGCCACCAGACCGGCGACCGCATCCTGCAG
CTGGTGGCGGAAACCATCACCTCCCACCTGCGCCGCTCCGACGTGGTTGGGCGCTACGACGGCGACGAATTCGTGGTCTA
CCTGTCGCCGGTGGAACCGGCGTCGCTCCGGACCGTGGCCGAGAACCTGCGGCGCAGCATTGAGGAGGAAAGCGCCCGCA
TGGTGCCGGTCACCGCCAGCATCGGCGTTGCCCAGGGCATTCTCGGCACCGACGTGGACGGGGGGATCGAAGAGCTGGTC
CGCCTGGCCGACGAGTGTCTCATGCAGGCCAAGTACACCGGCAAGAACAAGGTTGTCGTGAAATAG

Upstream 100 bases:

>100_bases
AATCGCCTCGGCTCAGAATTCTAATGAAATAATCGGGCGAGGAGCGTATACTTTTTCCGACGCTGGTTCCGCCGATTTCC
CTGGGGGCAGGAGGTGCCGC

Downstream 100 bases:

>100_bases
CGCTGCCGGCGGTCCAGGCCGCGGTCAGAACCCTACCCCCACCCCTATGCCGAAGTGCACCGCCGGCTGGGCATAGGGAT
CCTCAGGTCTCCAGACGTAC

Product: GGDEF domain-containing protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MSGDILNDIVAACLELERKASSVFKMFAAHAGSDEARRFWETVADETRHHSAVYERLQERGGRENLPIIIYKPAETLEEL
EMIGKSIDEQVERYTEAPSSEAACLLGFRLQLYLLHPAFASLCRLTRDASGEDLPDIGYGRYLRRFIDGIGSCGLATAET
ELLGEALFRLWNEARQLAAQSHFDALTGVMTRAGFFKTVGSLAYAAQRSGSNVGIMLIDLDYFKLVGDNYGHQTGDRILQ
LVAETITSHLRRSDVVGRYDGDEFVVYLSPVEPASLRTVAENLRRSIEEESARMVPVTASIGVAQGILGTDVDGGIEELV
RLADECLMQAKYTGKNKVVVK

Sequences:

>Translated_341_residues
MSGDILNDIVAACLELERKASSVFKMFAAHAGSDEARRFWETVADETRHHSAVYERLQERGGRENLPIIIYKPAETLEEL
EMIGKSIDEQVERYTEAPSSEAACLLGFRLQLYLLHPAFASLCRLTRDASGEDLPDIGYGRYLRRFIDGIGSCGLATAET
ELLGEALFRLWNEARQLAAQSHFDALTGVMTRAGFFKTVGSLAYAAQRSGSNVGIMLIDLDYFKLVGDNYGHQTGDRILQ
LVAETITSHLRRSDVVGRYDGDEFVVYLSPVEPASLRTVAENLRRSIEEESARMVPVTASIGVAQGILGTDVDGGIEELV
RLADECLMQAKYTGKNKVVVK
>Mature_340_residues
SGDILNDIVAACLELERKASSVFKMFAAHAGSDEARRFWETVADETRHHSAVYERLQERGGRENLPIIIYKPAETLEELE
MIGKSIDEQVERYTEAPSSEAACLLGFRLQLYLLHPAFASLCRLTRDASGEDLPDIGYGRYLRRFIDGIGSCGLATAETE
LLGEALFRLWNEARQLAAQSHFDALTGVMTRAGFFKTVGSLAYAAQRSGSNVGIMLIDLDYFKLVGDNYGHQTGDRILQL
VAETITSHLRRSDVVGRYDGDEFVVYLSPVEPASLRTVAENLRRSIEEESARMVPVTASIGVAQGILGTDVDGGIEELVR
LADECLMQAKYTGKNKVVVK

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI87082007, Length=167, Percent_Identity=35.3293413173653, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787262, Length=167, Percent_Identity=34.7305389221557, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI87081881, Length=167, Percent_Identity=34.7305389221557, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787802, Length=171, Percent_Identity=32.1637426900585, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI145693134, Length=160, Percent_Identity=35.625, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081977, Length=127, Percent_Identity=36.2204724409449, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788085, Length=182, Percent_Identity=28.021978021978, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788381, Length=167, Percent_Identity=31.7365269461078, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786584, Length=170, Percent_Identity=31.1764705882353, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87081974, Length=164, Percent_Identity=29.2682926829268, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787816, Length=163, Percent_Identity=25.7668711656442, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 37662; Mature: 37531

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGDILNDIVAACLELERKASSVFKMFAAHAGSDEARRFWETVADETRHHSAVYERLQER
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGRENLPIIIYKPAETLEELEMIGKSIDEQVERYTEAPSSEAACLLGFRLQLYLLHPAFA
CCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
SLCRLTRDASGEDLPDIGYGRYLRRFIDGIGSCGLATAETELLGEALFRLWNEARQLAAQ
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SHFDALTGVMTRAGFFKTVGSLAYAAQRSGSNVGIMLIDLDYFKLVGDNYGHQTGDRILQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHCCCCCCHHHHHHHH
LVAETITSHLRRSDVVGRYDGDEFVVYLSPVEPASLRTVAENLRRSIEEESARMVPVTAS
HHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEHH
IGVAQGILGTDVDGGIEELVRLADECLMQAKYTGKNKVVVK
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEC
>Mature Secondary Structure 
SGDILNDIVAACLELERKASSVFKMFAAHAGSDEARRFWETVADETRHHSAVYERLQER
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGRENLPIIIYKPAETLEELEMIGKSIDEQVERYTEAPSSEAACLLGFRLQLYLLHPAFA
CCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
SLCRLTRDASGEDLPDIGYGRYLRRFIDGIGSCGLATAETELLGEALFRLWNEARQLAAQ
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SHFDALTGVMTRAGFFKTVGSLAYAAQRSGSNVGIMLIDLDYFKLVGDNYGHQTGDRILQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHCCCCCCHHHHHHHH
LVAETITSHLRRSDVVGRYDGDEFVVYLSPVEPASLRTVAENLRRSIEEESARMVPVTAS
HHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEHH
IGVAQGILGTDVDGGIEELVRLADECLMQAKYTGKNKVVVK
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]