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The map label for this gene is polA [H]

Identifier: 39995648

GI number: 39995648

Start: 572574

End: 575249

Strand: Direct

Name: polA [H]

Synonym: GSU0541

Alternate gene names: 39995648

Gene position: 572574-575249 (Clockwise)

Preceding gene: 39995647

Following gene: 39995649

Centisome position: 15.01

GC content: 66.52

Gene sequence:

>2676_bases
ATGGCGACCGACACGCTGTTTCTCATCGACGGCTCCTCGTACATCTACCGGGCCTACTTCGCCATCAGGCATCTGTCGTC
GCCCGCGGGTTTCCCCACCAATGCGCTCTACGGCTTCACCCAGATGCTCCTCAAGGTCATCAAGGATCACCATCCCGGCC
GCTTGGCCGTGGTCTTCGACAAGGGGCGCACCACCTTCCGCACCGAGATCTACCCCGATTACAAGGCGAACCGGGCCGCC
ATGCCCGATGATCTGGTTCCCCAGATCGGACCCATCAAGGAGATGGTCCGGGCCTTCAGCATCCCGGTTCTGGAGTTGGA
GGGCTACGAGGCCGACGACATCATCGGCACCATTGCCCGGCGGTGCGAGGAGCAGGGTTTGGAGGCCGTGGTCGTCACCG
GCGACAAGGATCTGATGCAGATCGTGAGCGACCGCATCCGGCTGCTCGACACCATGAAGGACCGGGTGTCCGGCATCCCC
GAGGTGGTGGAGCGCTTCGGCGTCGGCCCCGGGCAGGTCATCGACATACTGGGCCTGGCGGGCGACACCTCCGACAACAT
CCCCGGCGTCCCCGGCATCGGCGAAAAGACCGCCACCAAGCTGATCCAGGAGTTTGGCTCTCTGGACGCCCTGCTGGAGC
GGGCCGGCGAGGTTAAGGGAAAGACCGGCGAGCGCCTGCGCGAGTTCGCGGAGCAGGCCCGGCTTTCCCGGCGTCTCGCC
ACCATTGTCCGCGACGTGCCCCTGGACTTCGACCTCGACGCCTTTGCCGTGGCCCCCGCCGACAATCGCCGATTGGCCGA
ACTCTTCAAGGAATATGGGTTCACGACCCTCATGAAGGAGCTGACCAGCGAGGCCACCCTGACCACCGAGCACTACCGCA
CCATTTTCGCGGAGGCCGATTTCCGGGCCCTGCTCGCCGACCTGGCCGGGGCCGGGGTCTTTTCGGTGGACCTGGAAACC
ACGAGCCTCAATCCTCTTGAGGCGGACATCGTGGGCATCTCCCTGTCGTTCCGGGATCACGAAGCCTGCTACATCCCGGT
GGGGCACGCCTATGACGGCGCCCCGGCCCAGCTCGACCGCACCCTGGTGCTGGAGGGGCTGCGGCCGCTCCTCACCGACC
CCGCGGTGCGCAAGGTGGGGCAGAACCTCAAGTACGACTACCAGGTGCTGCGCCGTCACGGCATCGTCATGGCCGGGGTC
TGGTGCGATACCATGGTCGCCTCCTATCTGATCAACCCGGTCCGGTCGGGCCACGGCCTGGACTCCCTGGCGGTGGAGCA
CCTGGACCACCGGATGATCTCCTACGAGGAGGTGGCCGGGAAGGGAAAGGACCAGCTGAACTTTGCCCGGGTGCCGGTGG
AAAAGGCGTCCACCTATTCCTGCGAGGATGCCGACGCCGCCTGGCTCCTGCACCGGCTGTTCCTGCCCCGGGTGGCCGAG
GCCGGCATGGAGCGCCTGTTCTTCGATCTGGAGATGCCGCTCGTACCGATCCTGGCCGAGATGGAAACGGCCGGGGTCAA
GCTGGACCTGGCGCTGCTGGGGGAACTCTCCGCCGGGCTCGGCAGCCAGTTGACGGCTCTGGAGGAGCAGGTCATGGCGC
TCGCGCCCGAGCCGTTCAACCTCAACTCGCCCAAGCAGCTGGGAGAGGTGCTGTTCGAGAAAATGAAGCTCCCAGCCGGG
AAAAAGACCAAGACCAAAACCGGCTGGTCCACCAACGTGGAGGAGCTGGAACGGCTGGCCGAGGCCGGGCACGAGATCGC
CGCGGCCATCCTCCGGTACCGGGGGCTCGCCAAGCTCAAGTCCACCTACACCGATGCCCTGCCCAGGCTGGTGCACCCGG
CCAGCGGGAGGGTGCACACCTCCTACAACCAGACCGTCACCAACACCGGGCGGCTCTCGTCCTCGGAGCCCAACCTGCAG
AACATCCCGGTCCGCACCGACGAGGGGCGGAAGATCCGGCGCGCCTTCATCGCGGAGGAGGGCCACCTGATCCTCTCGGC
CGACTACTCCCAGATCGAGCTGCGGGTCCTGGCCCATCTCTCGGAGGACCGGGTCTTCTGCGACGCCTTTGCCCGGGACG
AGGACATCCACACCCGGACCGCGGCCGAGGTGTTCGGCCTCTTCCCGGAGATGGTGACGCCCGAGATGAGGCGCCAGGCC
AAGGCCATCAACTTCGGGGTCATCTACGGACAGGGGGCCTTCAGTCTGGCCAAGCAACTGGGGATCACCACCAAGGTGGC
CAAGGAGTTCATCGACAACTACTTCGCCCGCCATCCTGGCGCCCGGGCCTTCCTGGACGGCTGCGTGGCCGAGGCCGAGG
CCAGGGGCTACGTGACCACCCTCATGGGGCGGCGGCTCCCCATTCCGGACATCGCCAGCAGCAACGGCAACATCCGGGCC
TTTGCCCAGCGCAACGCCGTCAACTACCCCATTCAGGGCTCGGCCGCGGACATCATCAAGGCGGCCATGGTGCGGGTCAC
GGAGCGGATGCGGCGAGAGGGGCTCACCAGTCGCCTGATCATGCAGGTCCACGACGAACTGGTCTTCGAGGTGCCGGAGG
GCGAACGGACCGCCATGGAGCGGCTCGTGCAACACGAGATGGAGCATGCTGCCCCCCTCCGGGTGCCGCTGCGGGCGGAC
GTGAACGTGGGGCGTAACTGGAGCGAGGCCCACTGA

Upstream 100 bases:

>100_bases
GGGGTGGCGCTTCGACGGGATGCAGTTCGCCATGCGCGAGGCGAGCCGTCGTCCCTCCATGGCGTTTCTCATCTTCACCC
GCGAAGCTGAGGAGGCGTAG

Downstream 100 bases:

>100_bases
ACCGGAATGCCTGAACCTGAAACTGCAGGGGGGGACCGTTGCCACGGCGGCAGGTAGTGTTCAACTGCCGAATATGCGTC
GAATTTTTTAATCGCCTCGG

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 891; Mature: 890

Protein sequence:

>891_residues
MATDTLFLIDGSSYIYRAYFAIRHLSSPAGFPTNALYGFTQMLLKVIKDHHPGRLAVVFDKGRTTFRTEIYPDYKANRAA
MPDDLVPQIGPIKEMVRAFSIPVLELEGYEADDIIGTIARRCEEQGLEAVVVTGDKDLMQIVSDRIRLLDTMKDRVSGIP
EVVERFGVGPGQVIDILGLAGDTSDNIPGVPGIGEKTATKLIQEFGSLDALLERAGEVKGKTGERLREFAEQARLSRRLA
TIVRDVPLDFDLDAFAVAPADNRRLAELFKEYGFTTLMKELTSEATLTTEHYRTIFAEADFRALLADLAGAGVFSVDLET
TSLNPLEADIVGISLSFRDHEACYIPVGHAYDGAPAQLDRTLVLEGLRPLLTDPAVRKVGQNLKYDYQVLRRHGIVMAGV
WCDTMVASYLINPVRSGHGLDSLAVEHLDHRMISYEEVAGKGKDQLNFARVPVEKASTYSCEDADAAWLLHRLFLPRVAE
AGMERLFFDLEMPLVPILAEMETAGVKLDLALLGELSAGLGSQLTALEEQVMALAPEPFNLNSPKQLGEVLFEKMKLPAG
KKTKTKTGWSTNVEELERLAEAGHEIAAAILRYRGLAKLKSTYTDALPRLVHPASGRVHTSYNQTVTNTGRLSSSEPNLQ
NIPVRTDEGRKIRRAFIAEEGHLILSADYSQIELRVLAHLSEDRVFCDAFARDEDIHTRTAAEVFGLFPEMVTPEMRRQA
KAINFGVIYGQGAFSLAKQLGITTKVAKEFIDNYFARHPGARAFLDGCVAEAEARGYVTTLMGRRLPIPDIASSNGNIRA
FAQRNAVNYPIQGSAADIIKAAMVRVTERMRREGLTSRLIMQVHDELVFEVPEGERTAMERLVQHEMEHAAPLRVPLRAD
VNVGRNWSEAH

Sequences:

>Translated_891_residues
MATDTLFLIDGSSYIYRAYFAIRHLSSPAGFPTNALYGFTQMLLKVIKDHHPGRLAVVFDKGRTTFRTEIYPDYKANRAA
MPDDLVPQIGPIKEMVRAFSIPVLELEGYEADDIIGTIARRCEEQGLEAVVVTGDKDLMQIVSDRIRLLDTMKDRVSGIP
EVVERFGVGPGQVIDILGLAGDTSDNIPGVPGIGEKTATKLIQEFGSLDALLERAGEVKGKTGERLREFAEQARLSRRLA
TIVRDVPLDFDLDAFAVAPADNRRLAELFKEYGFTTLMKELTSEATLTTEHYRTIFAEADFRALLADLAGAGVFSVDLET
TSLNPLEADIVGISLSFRDHEACYIPVGHAYDGAPAQLDRTLVLEGLRPLLTDPAVRKVGQNLKYDYQVLRRHGIVMAGV
WCDTMVASYLINPVRSGHGLDSLAVEHLDHRMISYEEVAGKGKDQLNFARVPVEKASTYSCEDADAAWLLHRLFLPRVAE
AGMERLFFDLEMPLVPILAEMETAGVKLDLALLGELSAGLGSQLTALEEQVMALAPEPFNLNSPKQLGEVLFEKMKLPAG
KKTKTKTGWSTNVEELERLAEAGHEIAAAILRYRGLAKLKSTYTDALPRLVHPASGRVHTSYNQTVTNTGRLSSSEPNLQ
NIPVRTDEGRKIRRAFIAEEGHLILSADYSQIELRVLAHLSEDRVFCDAFARDEDIHTRTAAEVFGLFPEMVTPEMRRQA
KAINFGVIYGQGAFSLAKQLGITTKVAKEFIDNYFARHPGARAFLDGCVAEAEARGYVTTLMGRRLPIPDIASSNGNIRA
FAQRNAVNYPIQGSAADIIKAAMVRVTERMRREGLTSRLIMQVHDELVFEVPEGERTAMERLVQHEMEHAAPLRVPLRAD
VNVGRNWSEAH
>Mature_890_residues
ATDTLFLIDGSSYIYRAYFAIRHLSSPAGFPTNALYGFTQMLLKVIKDHHPGRLAVVFDKGRTTFRTEIYPDYKANRAAM
PDDLVPQIGPIKEMVRAFSIPVLELEGYEADDIIGTIARRCEEQGLEAVVVTGDKDLMQIVSDRIRLLDTMKDRVSGIPE
VVERFGVGPGQVIDILGLAGDTSDNIPGVPGIGEKTATKLIQEFGSLDALLERAGEVKGKTGERLREFAEQARLSRRLAT
IVRDVPLDFDLDAFAVAPADNRRLAELFKEYGFTTLMKELTSEATLTTEHYRTIFAEADFRALLADLAGAGVFSVDLETT
SLNPLEADIVGISLSFRDHEACYIPVGHAYDGAPAQLDRTLVLEGLRPLLTDPAVRKVGQNLKYDYQVLRRHGIVMAGVW
CDTMVASYLINPVRSGHGLDSLAVEHLDHRMISYEEVAGKGKDQLNFARVPVEKASTYSCEDADAAWLLHRLFLPRVAEA
GMERLFFDLEMPLVPILAEMETAGVKLDLALLGELSAGLGSQLTALEEQVMALAPEPFNLNSPKQLGEVLFEKMKLPAGK
KTKTKTGWSTNVEELERLAEAGHEIAAAILRYRGLAKLKSTYTDALPRLVHPASGRVHTSYNQTVTNTGRLSSSEPNLQN
IPVRTDEGRKIRRAFIAEEGHLILSADYSQIELRVLAHLSEDRVFCDAFARDEDIHTRTAAEVFGLFPEMVTPEMRRQAK
AINFGVIYGQGAFSLAKQLGITTKVAKEFIDNYFARHPGARAFLDGCVAEAEARGYVTTLMGRRLPIPDIASSNGNIRAF
AQRNAVNYPIQGSAADIIKAAMVRVTERMRREGLTSRLIMQVHDELVFEVPEGERTAMERLVQHEMEHAAPLRVPLRADV
NVGRNWSEAH

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=515, Percent_Identity=32.0388349514563, Blast_Score=247, Evalue=3e-65,
Organism=Homo sapiens, GI139394648, Length=696, Percent_Identity=27.0114942528736, Blast_Score=203, Evalue=7e-52,
Organism=Escherichia coli, GI1790294, Length=924, Percent_Identity=47.1861471861472, Blast_Score=774, Evalue=0.0,
Organism=Escherichia coli, GI226510970, Length=241, Percent_Identity=32.3651452282158, Blast_Score=126, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI86563272, Length=409, Percent_Identity=32.7628361858191, Blast_Score=198, Evalue=9e-51,
Organism=Drosophila melanogaster, GI17933644, Length=593, Percent_Identity=28.3305227655987, Blast_Score=202, Evalue=1e-51,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01612 3_5_exonuc; PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 98597; Mature: 98466

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATDTLFLIDGSSYIYRAYFAIRHLSSPAGFPTNALYGFTQMLLKVIKDHHPGRLAVVFD
CCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEE
KGRTTFRTEIYPDYKANRAAMPDDLVPQIGPIKEMVRAFSIPVLELEGYEADDIIGTIAR
CCCCEEEEEECCCCCCCCCCCCHHCCCCCCCHHHHHHHHCCCEEEECCCCHHHHHHHHHH
RCEEQGLEAVVVTGDKDLMQIVSDRIRLLDTMKDRVSGIPEVVERFGVGPGQVIDILGLA
HHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHCCC
GDTSDNIPGVPGIGEKTATKLIQEFGSLDALLERAGEVKGKTGERLREFAEQARLSRRLA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
TIVRDVPLDFDLDAFAVAPADNRRLAELFKEYGFTTLMKELTSEATLTTEHYRTIFAEAD
HHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHCHHHHHHHHHHHHHEEHHHHHHHHHHHH
FRALLADLAGAGVFSVDLETTSLNPLEADIVGISLSFRDHEACYIPVGHAYDGAPAQLDR
HHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEEEEEECCCCEEEEECCCCCCCCCHHHHH
TLVLEGLRPLLTDPAVRKVGQNLKYDYQVLRRHGIVMAGVWCDTMVASYLINPVRSGHGL
HHHHHCCHHHHCCHHHHHHCCCCCHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCCH
DSLAVEHLDHRMISYEEVAGKGKDQLNFARVPVEKASTYSCEDADAAWLLHRLFLPRVAE
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHCCCCCCCCCHHHHHHHHHHHHHHHH
AGMERLFFDLEMPLVPILAEMETAGVKLDLALLGELSAGLGSQLTALEEQVMALAPEPFN
HHHHHHHHHHCCCHHHHHHHHHHCCCEEHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
LNSPKQLGEVLFEKMKLPAGKKTKTKTGWSTNVEELERLAEAGHEIAAAILRYRGLAKLK
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STYTDALPRLVHPASGRVHTSYNQTVTNTGRLSSSEPNLQNIPVRTDEGRKIRRAFIAEE
HHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCC
GHLILSADYSQIELRVLAHLSEDRVFCDAFARDEDIHTRTAAEVFGLFPEMVTPEMRRQA
CCEEEECCCHHHHHHHHHHHCCCCEEHHHHCCCCCHHHHHHHHHHHHCHHHCCHHHHHHH
KAINFGVIYGQGAFSLAKQLGITTKVAKEFIDNYFARHPGARAFLDGCVAEAEARGYVTT
HHEEEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHH
LMGRRLPIPDIASSNGNIRAFAQRNAVNYPIQGSAADIIKAAMVRVTERMRREGLTSRLI
HHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHH
MQVHDELVFEVPEGERTAMERLVQHEMEHAAPLRVPLRADVNVGRNWSEAH
HHHHHHHHEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
ATDTLFLIDGSSYIYRAYFAIRHLSSPAGFPTNALYGFTQMLLKVIKDHHPGRLAVVFD
CCCEEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEE
KGRTTFRTEIYPDYKANRAAMPDDLVPQIGPIKEMVRAFSIPVLELEGYEADDIIGTIAR
CCCCEEEEEECCCCCCCCCCCCHHCCCCCCCHHHHHHHHCCCEEEECCCCHHHHHHHHHH
RCEEQGLEAVVVTGDKDLMQIVSDRIRLLDTMKDRVSGIPEVVERFGVGPGQVIDILGLA
HHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHCCC
GDTSDNIPGVPGIGEKTATKLIQEFGSLDALLERAGEVKGKTGERLREFAEQARLSRRLA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
TIVRDVPLDFDLDAFAVAPADNRRLAELFKEYGFTTLMKELTSEATLTTEHYRTIFAEAD
HHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHCHHHHHHHHHHHHHEEHHHHHHHHHHHH
FRALLADLAGAGVFSVDLETTSLNPLEADIVGISLSFRDHEACYIPVGHAYDGAPAQLDR
HHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEEEEEECCCCEEEEECCCCCCCCCHHHHH
TLVLEGLRPLLTDPAVRKVGQNLKYDYQVLRRHGIVMAGVWCDTMVASYLINPVRSGHGL
HHHHHCCHHHHCCHHHHHHCCCCCHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCCH
DSLAVEHLDHRMISYEEVAGKGKDQLNFARVPVEKASTYSCEDADAAWLLHRLFLPRVAE
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHCCCCCCCCCHHHHHHHHHHHHHHHH
AGMERLFFDLEMPLVPILAEMETAGVKLDLALLGELSAGLGSQLTALEEQVMALAPEPFN
HHHHHHHHHHCCCHHHHHHHHHHCCCEEHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
LNSPKQLGEVLFEKMKLPAGKKTKTKTGWSTNVEELERLAEAGHEIAAAILRYRGLAKLK
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STYTDALPRLVHPASGRVHTSYNQTVTNTGRLSSSEPNLQNIPVRTDEGRKIRRAFIAEE
HHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCC
GHLILSADYSQIELRVLAHLSEDRVFCDAFARDEDIHTRTAAEVFGLFPEMVTPEMRRQA
CCEEEECCCHHHHHHHHHHHCCCCEEHHHHCCCCCHHHHHHHHHHHHCHHHCCHHHHHHH
KAINFGVIYGQGAFSLAKQLGITTKVAKEFIDNYFARHPGARAFLDGCVAEAEARGYVTT
HHEEEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHH
LMGRRLPIPDIASSNGNIRAFAQRNAVNYPIQGSAADIIKAAMVRVTERMRREGLTSRLI
HHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHH
MQVHDELVFEVPEGERTAMERLVQHEMEHAAPLRVPLRADVNVGRNWSEAH
HHHHHHHHEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]